##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename SRR25792494_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13689795 Sequences flagged as poor quality 0 Sequence length 101 %GC 62 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.834935219994165 35.0 35.0 35.0 31.0 35.0 2 33.89280964397202 35.0 35.0 35.0 31.0 35.0 3 33.96727606220546 35.0 35.0 35.0 31.0 35.0 4 34.040916463687005 35.0 35.0 35.0 31.0 35.0 5 34.007583020783 35.0 35.0 35.0 31.0 35.0 6 38.57950378365783 40.0 39.0 40.0 35.0 40.0 7 38.60680872138699 40.0 39.0 40.0 36.0 40.0 8 38.60538298783875 40.0 39.0 40.0 36.0 40.0 9 38.66088878613595 40.0 39.0 40.0 36.0 40.0 10-11 38.67059426382937 40.0 39.0 40.0 36.0 40.0 12-13 38.67902185533092 40.0 39.0 40.0 36.0 40.0 14-15 38.65453891018821 40.0 39.0 40.0 36.0 40.0 16-17 38.62903582559125 40.0 39.0 40.0 36.0 40.0 18-19 38.60856670972794 40.0 39.0 40.0 36.0 40.0 20-21 38.59546333601051 40.0 39.0 40.0 36.0 40.0 22-23 38.579149833872606 40.0 39.0 40.0 36.0 40.0 24-25 38.58738151301755 40.0 39.0 40.0 36.0 40.0 26-27 38.50953378045471 40.0 39.0 40.0 36.0 40.0 28-29 38.49859219951796 40.0 39.0 40.0 35.5 40.0 30-31 38.48261986392053 40.0 39.0 40.0 35.0 40.0 32-33 38.46941279982644 40.0 39.0 40.0 35.0 40.0 34-35 38.44879813759081 40.0 39.0 40.0 35.0 40.0 36-37 38.43921800874301 40.0 39.0 40.0 35.0 40.0 38-39 38.391428870921736 40.0 39.0 40.0 35.0 40.0 40-41 38.376531825348735 40.0 39.0 40.0 35.0 40.0 42-43 38.364554290257814 40.0 39.0 40.0 35.0 40.0 44-45 38.33743956721047 40.0 39.0 40.0 35.0 40.0 46-47 38.30555808176821 40.0 39.0 40.0 35.0 40.0 48-49 38.30053463181881 40.0 39.0 40.0 35.0 40.0 50-51 38.29256004198748 40.0 39.0 40.0 35.0 40.0 52-53 38.285520930006626 40.0 39.0 40.0 35.0 40.0 54-55 38.23684452542935 40.0 39.0 40.0 35.0 40.0 56-57 38.21306670406679 40.0 39.0 40.0 35.0 40.0 58-59 38.205838911393485 40.0 39.0 40.0 35.0 40.0 60-61 38.20784628257765 40.0 39.0 40.0 35.0 40.0 62-63 38.21299394914241 40.0 39.0 40.0 35.0 40.0 64-65 38.196993015600306 40.0 39.0 40.0 35.0 40.0 66-67 38.187943756645 40.0 39.0 40.0 35.0 40.0 68-69 38.16579857477778 40.0 39.0 40.0 35.0 40.0 70-71 38.13378827075205 40.0 39.0 40.0 34.5 40.0 72-73 38.117326556022206 40.0 39.0 40.0 34.0 40.0 74-75 38.081564625328575 40.0 39.0 40.0 34.0 40.0 76-77 38.070131656463815 40.0 39.0 40.0 34.0 40.0 78-79 38.03117146750554 40.0 39.0 40.0 34.0 40.0 80-81 38.02197406900542 40.0 39.0 40.0 34.0 40.0 82-83 37.978662901818474 40.0 39.0 40.0 34.0 40.0 84-85 37.95168086885158 40.0 39.0 40.0 34.0 40.0 86-87 37.92957038436295 40.0 39.0 40.0 34.0 40.0 88-89 37.89069500310268 40.0 39.0 40.0 34.0 40.0 90-91 37.887372016892876 40.0 39.0 40.0 34.0 40.0 92-93 37.87192368475934 40.0 39.0 40.0 34.0 40.0 94-95 37.82799505032764 40.0 39.0 40.0 34.0 40.0 96-97 37.78401955617305 40.0 39.0 40.0 34.0 40.0 98-99 37.6896066741686 40.0 38.0 40.0 34.0 40.0 100-101 36.608412580319865 39.5 36.5 39.5 30.5 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2583935845596699 1101 2 0.29510105536573406 1101 3 0.3235967699226805 1101 4 0.2973588168705845 1101 5 0.2776097801049673 1101 6 0.3418773485050295 1101 7 0.2952344885959022 1101 8 0.2974932650195896 1101 9 0.2341176832824985 1101 10-11 0.27835724053488065 1101 12-13 0.29785746403914715 1101 14-15 0.3189711973877891 1101 16-17 0.34509604720037856 1101 18-19 0.3419048999605181 1101 20-21 0.31693253546953315 1101 22-23 0.3364536011408319 1101 24-25 0.3517483243530961 1101 26-27 0.40164499293251055 1101 28-29 0.37148448183938854 1101 30-31 0.3636561239750833 1101 32-33 0.3867606847907794 1101 34-35 0.38769552340725966 1101 36-37 0.3704270373334211 1101 38-39 0.3911274724273852 1101 40-41 0.3613833909978368 1101 42-43 0.39762139614754943 1101 44-45 0.3859969817032223 1101 46-47 0.3727503815731339 1101 48-49 0.3648044980478531 1101 50-51 0.4040656172638677 1101 52-53 0.3674084569196907 1101 54-55 0.39551193352760095 1101 56-57 0.4038444528790279 1101 58-59 0.3725298223358635 1101 60-61 0.36538267393110857 1101 62-63 0.4096019771513042 1101 64-65 0.4192342890339589 1101 66-67 0.32219679621636743 1101 68-69 0.3791916259853565 1101 70-71 0.3854462818170816 1101 72-73 0.34454632249039463 1101 74-75 0.3850924662662152 1101 76-77 0.3538346786296671 1101 78-79 0.34734995087014653 1101 80-81 0.3672067360382769 1101 82-83 0.35855711392730427 1101 84-85 0.38614073964547657 1101 86-87 0.3497412811658833 1101 88-89 0.3478184062891927 1101 90-91 0.3074362620855027 1101 92-93 0.381068158506082 1101 94-95 0.38122042521074917 1101 96-97 0.35216190268299385 1101 98-99 0.35333963409473057 1101 100-101 0.2939573500160151 1102 1 0.2310038349739827 1102 2 0.28106033314734447 1102 3 0.25369455958079357 1102 4 0.25840331025865737 1102 5 0.24049119358594595 1102 6 0.2547102018142695 1102 7 0.17460547924432746 1102 8 0.16665281498915618 1102 9 0.15838574240250125 1102 10-11 0.19108640236470364 1102 12-13 0.22904782270116186 1102 14-15 0.31863640062587706 1102 16-17 0.275707710147465 1102 18-19 0.2500754538811307 1102 20-21 0.32143972467572723 1102 22-23 0.2919675162619768 1102 24-25 0.310189305791468 1102 26-27 0.30758977935490606 1102 28-29 0.3111917473195547 1102 30-31 0.31854754161835075 1102 32-33 0.2933703586184251 1102 34-35 0.3047895230712072 1102 36-37 0.32359345335569856 1102 38-39 0.34131698956567647 1102 40-41 0.33376185635696487 1102 42-43 0.3190452166373561 1102 44-45 0.3177445273752326 1102 46-47 0.3399148684354003 1102 48-49 0.3124573475503709 1102 50-51 0.2735438063215412 1102 52-53 0.26982365440585454 1102 54-55 0.3425699819316037 1102 56-57 0.2958056354185672 1102 58-59 0.31813929258029816 1102 60-61 0.3435135326129881 1102 62-63 0.32726553917411394 1102 64-65 0.340835129302711 1102 66-67 0.3094789290711404 1102 68-69 0.33737852931052714 1102 70-71 0.29621025567755765 1102 72-73 0.29863898979988335 1102 74-75 0.3477878311925977 1102 76-77 0.31813348787709117 1102 78-79 0.3135897377213226 1102 80-81 0.32919522452362315 1102 82-83 0.3174935625019799 1102 84-85 0.3163784753261538 1102 86-87 0.28960485950323545 1102 88-89 0.31105415563922634 1102 90-91 0.28406803026837224 1102 92-93 0.26281364742996516 1102 94-95 0.23465054074421943 1102 96-97 0.217781231374353 1102 98-99 0.22182450096037343 1102 100-101 0.21509141304174761 1103 1 0.2972566085175501 1103 2 0.27622930681918234 1103 3 0.2920936699913952 1103 4 0.28279338631599416 1103 5 0.2790999089663728 1103 6 0.3414025146968598 1103 7 0.30524653316079764 1103 8 0.32996919519199963 1103 9 0.30734825177052016 1103 10-11 0.3181844087495804 1103 12-13 0.3314847696854244 1103 14-15 0.354174526629059 1103 16-17 0.3385622567912492 1103 18-19 0.29446977461211077 1103 20-21 0.3063481810504953 1103 22-23 0.34059681564990285 1103 24-25 0.35331855729189243 1103 26-27 0.3737871110773412 1103 28-29 0.3343625151781282 1103 30-31 0.3382901545034187 1103 32-33 0.3630489127727756 1103 34-35 0.3045503864003294 1103 36-37 0.31436383425124603 1103 38-39 0.37655914654469314 1103 40-41 0.3870437188261846 1103 42-43 0.3653751620042911 1103 44-45 0.3464390790016907 1103 46-47 0.3991046230357824 1103 48-49 0.36131562768906633 1103 50-51 0.36472486416972316 1103 52-53 0.34820567586054096 1103 54-55 0.3710811550317814 1103 56-57 0.3700810668121193 1103 58-59 0.3865856751451062 1103 60-61 0.40142661343033126 1103 62-63 0.42186272653943035 1103 64-65 0.39197574009519087 1103 66-67 0.32019419428151963 1103 68-69 0.3468372995930906 1103 70-71 0.3166783790383363 1103 72-73 0.3003240462220518 1103 74-75 0.2836131466297118 1103 76-77 0.2837397504724066 1103 78-79 0.3401396187953125 1103 80-81 0.39287574985091567 1103 82-83 0.37832351885630544 1103 84-85 0.3781660409526211 1103 86-87 0.3730853465153743 1103 88-89 0.34913578057404493 1103 90-91 0.32817163681816197 1103 92-93 0.3958980290270091 1103 94-95 0.3416712084935156 1103 96-97 0.37689958595726125 1103 98-99 0.3557832910099279 1103 100-101 0.4586165892010001 1104 1 0.17897808354700828 1104 2 0.19657323036268792 1104 3 0.20932187181615802 1104 4 0.21887892627425032 1104 5 0.19540089710930886 1104 6 0.18580528000180152 1104 7 0.2502520123781835 1104 8 0.2184826480467379 1104 9 0.24056523083940817 1104 10-11 0.2578740389737888 1104 12-13 0.2344105363711364 1104 14-15 0.2644594577068915 1104 16-17 0.25884257255194143 1104 18-19 0.25705233448245934 1104 20-21 0.26748959489756885 1104 22-23 0.2833401419101449 1104 24-25 0.27080480332302415 1104 26-27 0.3028784587411124 1104 28-29 0.3137158954122654 1104 30-31 0.27655149418752245 1104 32-33 0.2814433799258751 1104 34-35 0.27719873808749895 1104 36-37 0.26138044968730867 1104 38-39 0.31096723359287637 1104 40-41 0.2902563975262282 1104 42-43 0.2875250932896378 1104 44-45 0.29103519901524066 1104 46-47 0.2901597381052312 1104 48-49 0.2716224506171798 1104 50-51 0.277197361383017 1104 52-53 0.3073618704962371 1104 54-55 0.32992223211964955 1104 56-57 0.25499181281868744 1104 58-59 0.283096467422574 1104 60-61 0.2710164491950877 1104 62-63 0.330965983369758 1104 64-65 0.2922800037004407 1104 66-67 0.23259598265804726 1104 68-69 0.321278398815636 1104 70-71 0.27864257324311126 1104 72-73 0.17538810287977213 1104 74-75 0.21704721926739268 1104 76-77 0.20484305263174463 1104 78-79 0.26835933968047954 1104 80-81 0.28218298280051357 1104 82-83 0.22045260762845942 1104 84-85 0.23374438610829174 1104 86-87 0.23415211467192165 1104 88-89 0.25704104177067677 1104 90-91 0.28266005481519585 1104 92-93 0.2901048273185154 1104 94-95 0.27999585425762064 1104 96-97 0.3025792120106061 1104 98-99 0.2914271454119799 1104 100-101 0.3894331544808054 1105 1 0.18986968621712208 1105 2 0.12449434964729278 1105 3 0.1646133581653757 1105 4 0.13226233019567246 1105 5 0.1411165770115801 1105 6 0.19351656653313398 1105 7 0.18109512064941669 1105 8 0.1800566473787697 1105 9 0.21183456585450955 1105 10-11 0.20941836164751493 1105 12-13 0.21202285314263492 1105 14-15 0.2229271471804566 1105 16-17 0.23199345312408326 1105 18-19 0.23584201562871243 1105 20-21 0.24905319417878502 1105 22-23 0.22346855749583483 1105 24-25 0.2234589310781061 1105 26-27 0.2371980108646241 1105 28-29 0.25848673615438145 1105 30-31 0.26290233683786113 1105 32-33 0.2476079370638118 1105 34-35 0.2524883838394416 1105 36-37 0.25365736505331427 1105 38-39 0.2953043989313002 1105 40-41 0.21111191292471432 1105 42-43 0.23673113694000847 1105 44-45 0.26564425512692225 1105 46-47 0.26051178229673155 1105 48-49 0.2492483118903337 1105 50-51 0.26353060713992704 1105 52-53 0.20978352200117456 1105 54-55 0.2739071373566375 1105 56-57 0.23527030726513232 1105 58-59 0.22803926667678098 1105 60-61 0.24690329811586764 1105 62-63 0.23836515179471718 1105 64-65 0.2598529744990614 1105 66-67 0.19788264670767575 1105 68-69 0.19887642962369512 1105 70-71 0.19938701859845054 1105 72-73 0.1837662247020404 1105 74-75 0.23314339404270612 1105 76-77 0.19029282568381944 1105 78-79 0.24685140785802417 1105 80-81 0.21424397936432626 1105 82-83 0.26731452683188195 1105 84-85 0.2334796446121814 1105 86-87 0.17686282715327906 1105 88-89 0.2644085698294134 1105 90-91 0.25121554875325813 1105 92-93 0.32802579691177414 1105 94-95 0.2866640252989541 1105 96-97 0.25803251656589765 1105 98-99 0.27313695955875517 1105 100-101 0.3225570435122336 1106 1 0.13377805812164212 1106 2 0.08436597799775569 1106 3 0.1214684174477938 1106 4 0.08853510332443904 1106 5 0.13986040877695416 1106 6 0.19324429357111939 1106 7 0.15525262504864656 1106 8 0.18850124677476998 1106 9 0.18325661412072236 1106 10-11 0.1653961029182156 1106 12-13 0.14268567978837865 1106 14-15 0.19130117544014524 1106 16-17 0.13699202483189055 1106 18-19 0.11854067730161688 1106 20-21 0.17431828488685852 1106 22-23 0.1454908439643603 1106 24-25 0.12131336114451585 1106 26-27 0.1758684678953486 1106 28-29 0.19859228570116727 1106 30-31 0.21138090082674665 1106 32-33 0.13892118830349887 1106 34-35 0.15601449935214617 1106 36-37 0.19027505438441494 1106 38-39 0.23908821552635118 1106 40-41 0.18884312658791913 1106 42-43 0.10703448662095383 1106 44-45 0.12565894310967707 1106 46-47 0.13110717832761054 1106 48-49 0.12785702018540945 1106 50-51 0.16147283400730572 1106 52-53 0.13089854796317013 1106 54-55 0.1723477513234215 1106 56-57 0.1348519613696837 1106 58-59 0.16458911638738272 1106 60-61 0.17414103642329337 1106 62-63 0.184690413563267 1106 64-65 0.1746671102112245 1106 66-67 0.14467706807862868 1106 68-69 0.16116813282847886 1106 70-71 0.1586840061167507 1106 72-73 0.1326815268405781 1106 74-75 0.18426472444752307 1106 76-77 0.149940830061702 1106 78-79 0.13922723102672308 1106 80-81 0.10091714131921492 1106 82-83 0.1364611265954352 1106 84-85 0.1706485457090423 1106 86-87 0.18222919284187356 1106 88-89 0.140517567306361 1106 90-91 0.18914994354799575 1106 92-93 0.19184704810290043 1106 94-95 0.2034266600634922 1106 96-97 0.21315929612374163 1106 98-99 0.23481122292407264 1106 100-101 0.30806587791768436 1107 1 0.08414267896117877 1107 2 0.11756359000754202 1107 3 0.07579870248234499 1107 4 0.0481721252291436 1107 5 0.07924741901450005 1107 6 0.11391095543566365 1107 7 -0.05824597105496565 1107 8 0.053233898664380774 1107 9 -0.018274284750674497 1107 10-11 0.0704725584301471 1107 12-13 0.09005235212057272 1107 14-15 0.08660987376744345 1107 16-17 0.05226352177080429 1107 18-19 0.11099769661370118 1107 20-21 0.1373163842354188 1107 22-23 0.15879298307091005 1107 24-25 0.15219738737180677 1107 26-27 0.18549394362624838 1107 28-29 0.12110170739029513 1107 30-31 0.1263086594412428 1107 32-33 0.0661555416098949 1107 34-35 0.09737701033493806 1107 36-37 0.09407220522152215 1107 38-39 0.14600038190344833 1107 40-41 0.1366761467794646 1107 42-43 0.1154028222555823 1107 44-45 0.12156859596469616 1107 46-47 0.1201176681263263 1107 48-49 0.07576900392820818 1107 50-51 0.10121139398930978 1107 52-53 0.0709400187835314 1107 54-55 0.09729204651433321 1107 56-57 0.11318614067951671 1107 58-59 0.13274816622293173 1107 60-61 0.08733252554835502 1107 62-63 0.1222380581207787 1107 64-65 0.1397751618745886 1107 66-67 0.06533607058079838 1107 68-69 0.08034536260158376 1107 70-71 0.1143660328446785 1107 72-73 0.0652668734037789 1107 74-75 0.12511985915156032 1107 76-77 0.041776269308407166 1107 78-79 0.10871010150793126 1107 80-81 0.06709892871270995 1107 82-83 0.07804010905830694 1107 84-85 0.08897915201104922 1107 86-87 0.08330561260722646 1107 88-89 0.04653314188769997 1107 90-91 0.026459018517805077 1107 92-93 0.09893546779085227 1107 94-95 0.036979083889356446 1107 96-97 0.04448863998209873 1107 98-99 0.15306660303528474 1107 100-101 0.17806033906477126 1108 1 0.009973365340094631 1108 2 0.051635427949051405 1108 3 0.062119230187555274 1108 4 0.08429724842962116 1108 5 0.10294134963385915 1108 6 0.10536183446605918 1108 7 0.07293329243279345 1108 8 0.08901467931426055 1108 9 0.09947859340772425 1108 10-11 0.0839900759493517 1108 12-13 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2214 84-85 -0.3205579695075329 2214 86-87 -0.3296611595205121 2214 88-89 -0.3765650611425002 2214 90-91 -0.367960690814364 2214 92-93 -0.33316781756666813 2214 94-95 -0.2731460059641151 2214 96-97 -0.2732691570499739 2214 98-99 -0.2647371687562341 2214 100-101 -0.2608960060346277 2215 1 -0.3109673146416583 2215 2 -0.3640577827487519 2215 3 -0.3920732296206566 2215 4 -0.33465799205883684 2215 5 -0.36171330531990975 2215 6 -0.47882156448847724 2215 7 -0.39018287259654727 2215 8 -0.37904475487170686 2215 9 -0.4624666129148167 2215 10-11 -0.4068454866806448 2215 12-13 -0.45583081923480506 2215 14-15 -0.42060171324569495 2215 16-17 -0.4284265159642118 2215 18-19 -0.4506131311417647 2215 20-21 -0.4377668194791724 2215 22-23 -0.4721722974494327 2215 24-25 -0.4642489619233672 2215 26-27 -0.4673388062018873 2215 28-29 -0.4712226104442294 2215 30-31 -0.5070263418007315 2215 32-33 -0.4734901865863961 2215 34-35 -0.45216422493163577 2215 36-37 -0.49984172595209486 2215 38-39 -0.42471025100199 2215 40-41 -0.502495850576544 2215 42-43 -0.46681673894165243 2215 44-45 -0.485192983641916 2215 46-47 -0.48824314627439236 2215 48-49 -0.4448609270781603 2215 50-51 -0.4833389694287007 2215 52-53 -0.49903180878017395 2215 54-55 -0.4625339652798246 2215 56-57 -0.4542266664073651 2215 58-59 -0.4395791880441138 2215 60-61 -0.47145575728887934 2215 62-63 -0.46266938842557437 2215 64-65 -0.47422318373235584 2215 66-67 -0.45819400607902594 2215 68-69 -0.4552297111097232 2215 70-71 -0.4358933083512895 2215 72-73 -0.46200760786519623 2215 74-75 -0.4685974707780858 2215 76-77 -0.4628553814751655 2215 78-79 -0.45398239697778564 2215 80-81 -0.4113041394558081 2215 82-83 -0.4045332098874752 2215 84-85 -0.3432062967634266 2215 86-87 -0.38194803503107266 2215 88-89 -0.4146951964050203 2215 90-91 -0.43595584728527825 2215 92-93 -0.4642090160979535 2215 94-95 -0.4243454784420919 2215 96-97 -0.8454124032013013 2215 98-99 -0.46159285703632946 2215 100-101 -0.4795243790753787 2216 1 -0.4694924410788417 2216 2 -0.49787774934657847 2216 3 -0.43070636659174255 2216 4 -0.4333990859259842 2216 5 -0.43970774899219833 2216 6 -0.5494518188057782 2216 7 -0.4992516851561746 2216 8 -0.49459998404383754 2216 9 -0.612631511004281 2216 10-11 -0.5767613762280277 2216 12-13 -0.5944531266215947 2216 14-15 -0.6098892874285085 2216 16-17 -0.6938005015759998 2216 18-19 -0.694990538836187 2216 20-21 -0.6469133794384945 2216 22-23 -0.6245756641979909 2216 24-25 -0.7137281626764249 2216 26-27 -0.9184136016122579 2216 28-29 -0.7229624519515099 2216 30-31 -0.6175259471647436 2216 32-33 -0.6516874466343907 2216 34-35 -0.6657308337657568 2216 36-37 -0.6721312307504945 2216 38-39 -0.7134639001614076 2216 40-41 -0.6433160012332522 2216 42-43 -0.671197588302725 2216 44-45 -0.7003108802986091 2216 46-47 -0.6409342720083941 2216 48-49 -0.6560913013127205 2216 50-51 -0.6553197852595858 2216 52-53 -0.7256260545763951 2216 54-55 -0.7075014126958834 2216 56-57 -0.7491548127127174 2216 58-59 -0.8313714948661612 2216 60-61 -0.6962021567896386 2216 62-63 -0.7264787889881958 2216 64-65 -0.700440797060125 2216 66-67 -0.7301992237780652 2216 68-69 -0.7372548262539738 2216 70-71 -0.7679513642837961 2216 72-73 -0.7297068165725022 2216 74-75 -0.8597363205940098 2216 76-77 -0.7688789234392459 2216 78-79 -0.7607843057861245 2216 80-81 -0.7269375743447384 2216 82-83 -0.7607699581722329 2216 84-85 -0.7063056552435967 2216 86-87 -0.7605519890289045 2216 88-89 -0.7228247188765025 2216 90-91 -0.7340876670210079 2216 92-93 -0.773328731338836 2216 94-95 -0.8053135412962789 2216 96-97 -0.7687327483538056 2216 98-99 -0.7502681031479668 2216 100-101 -1.0130253301646235 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 28.0 17 124.0 18 109.0 19 117.0 20 215.0 21 772.0 22 2266.0 23 5619.0 24 11944.0 25 22095.0 26 37795.0 27 58259.0 28 85100.0 29 116933.0 30 154324.0 31 198670.0 32 248760.0 33 311755.0 34 391849.0 35 503201.0 36 675622.0 37 1000647.0 38 1974275.0 39 7889316.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.39215103162558 7.995230086784293 5.072574170978536 52.54004471061159 2 28.41920569299979 7.9704115364766235 29.330746004596854 34.27963676592674 3 25.820554654032442 10.79920480912972 14.968492954058114 48.41174758277973 4 38.354993628465586 12.71866379299325 15.440793671490333 33.48554890705084 5 37.44044237391732 17.389252565535397 20.063922610278446 25.10638245026884 6 31.502604677425776 23.294709672423874 19.460481329340578 25.74220432080977 7 30.426298007560675 15.809682780283799 34.02829227313403 19.735726939021493 8 23.891368313342632 17.98637976750687 27.429744858591963 30.692507060558526 9 26.74392137784537 14.3294705977744 31.018599276248416 27.90800874813182 10-11 28.34433240234788 21.86070353865781 21.39973608078134 28.395227978212965 12-13 29.906043151120965 15.62906530010128 22.416548239034988 32.04834330974277 14-15 31.904130047235917 17.185509352039237 21.33470223622779 29.57565836449706 16-17 32.77594733887542 18.50179275876666 19.81514697626955 28.907112926088374 18-19 29.747406736185606 19.047111370184872 21.152497170337465 30.05298472329206 20-21 30.284222663670274 17.43992148896313 21.612518668102773 30.663337179263824 22-23 30.27020492271798 19.038805913455974 20.366999651930506 30.323989511895537 24-25 29.928877678591974 19.234290944458994 21.170306056445696 29.66652532050334 26-27 30.91212834085536 16.96311011231359 19.662712261213553 32.4620492856175 28-29 33.23125364550747 17.859971606587244 18.14103498262757 30.767739765277714 30-31 30.298548630827284 16.918645706284323 20.027746280194915 32.755059382693474 32-33 33.143472930018305 16.722748587542764 19.000489050420406 31.133289432018525 34-35 32.424478687390085 16.49548501257634 19.93499975474431 31.14503654528927 36-37 32.982607771127675 15.271301071052104 17.88376767794418 33.86232347987604 38-39 32.454813969091575 16.843605035721865 19.07217383459723 31.62940716058933 40-41 31.960602770165657 17.04076649796436 21.151763046853514 29.846867685016466 42-43 30.237202237140874 16.156498325942792 21.278342736322934 32.3279567005934 44-45 29.518845972492645 17.242683327252163 21.550001296586252 31.68846940366894 46-47 30.89523619601316 15.37449245952916 21.709755332347928 32.02051601210975 48-49 29.705301552286457 17.41132532251173 20.05751592603048 32.82585719917134 50-51 27.58147948891857 19.15852647903055 19.96996667955948 33.2900273524914 52-53 31.49806114700768 15.980969766165234 18.82069819160915 33.70027089521793 54-55 32.203793942761195 17.07661392971642 18.543070376132718 32.17652175138967 56-57 29.50677128474166 16.091453524322315 19.995836314568628 34.4059388763674 58-59 29.75611489633935 16.826883673541243 19.096754466073413 34.320246964045985 60-61 29.23357873510889 16.93133827058769 19.128420111477197 34.70666288282622 62-63 27.85245871103256 17.35349944977262 20.252911018755213 34.541130820439605 64-65 28.99329756216218 17.230013305531603 20.028718472409558 33.74797065989666 66-67 27.729660222437964 16.88284656135634 20.826715112487626 34.560778103718064 68-69 28.970769832565058 18.573218225692933 20.30485847304507 32.15115346869693 70-71 32.84837928426195 17.81230676741819 18.25324502904903 31.086068919270826 72-73 31.96185790866427 18.430811559999817 17.958754274545978 31.64857625678993 74-75 32.209338089281744 19.479069007065075 17.41814544966719 30.893447453985996 76-77 29.272578319977004 19.932546916380893 18.602324479720426 32.192550283921676 78-79 28.32671944410386 19.43545140319019 19.472782248823144 32.765046903882805 80-81 27.207721052972595 19.73761150596078 21.12588852132243 31.928778919744193 82-83 29.867476256206704 18.328403788371222 19.480285064674916 32.32383489074716 84-85 30.659250434082626 18.5636960099461 17.73519535361755 33.04185820235372 86-87 30.042515157009213 18.321909177596655 18.31263578633464 33.32293987905949 88-89 28.764114289504395 20.23955348818727 18.189945025336822 32.80638719697152 90-91 29.008858716208586 17.42138340430835 20.498412905263585 33.07134497421948 92-93 31.117398032622113 16.45363937151725 19.680418881363817 32.74854371449682 94-95 31.191876868864725 17.1519149848482 18.966018117875397 32.69019002841168 96-97 29.093058004155655 17.11611459484967 21.406357801559484 32.3844695994352 98-99 27.78216648905869 19.81485551152722 20.070666510004955 32.332311489409136 100-101 28.24975100065414 18.90588208223717 18.50252688225061 34.34184003485809 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 30326.0 1 31821.5 2 34337.5 3 36326.0 4 36482.5 5 33888.5 6 29721.0 7 24982.5 8 20622.0 9 16555.0 10 13119.0 11 10600.0 12 8632.5 13 7112.5 14 6007.0 15 5194.0 16 4372.0 17 3738.0 18 3320.5 19 2942.5 20 2593.5 21 2276.0 22 2054.0 23 1869.5 24 1720.5 25 1574.5 26 1431.5 27 1376.5 28 1329.0 29 1254.0 30 1196.0 31 1128.0 32 1057.0 33 1020.5 34 992.5 35 957.5 36 1022.0 37 1077.5 38 1041.5 39 1041.5 40 1090.5 41 1204.0 42 1289.0 43 1407.0 44 1745.0 45 2505.0 46 5627.0 47 6583.0 48 5841.5 49 9967.0 50 21790.0 51 25779.0 52 25375.0 53 40241.0 54 79834.0 55 124776.0 56 160151.0 57 225200.0 58 357461.5 59 577443.5 60 1066249.0 61 1243350.5 62 1113914.5 63 1033619.0 64 983949.5 65 984704.0 66 809522.0 67 719281.5 68 693754.0 69 616589.5 70 539704.5 71 466662.5 72 372936.5 73 287055.0 74 222106.5 75 163151.0 76 112667.0 77 77767.0 78 53737.5 79 34100.0 80 20586.5 81 11657.5 82 6617.0 83 3894.0 84 2240.5 85 1062.5 86 438.0 87 139.5 88 49.0 89 18.0 90 4.0 91 3.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2743284322372979 2 0.0 3 0.0 4 0.0 5 0.005420095772069633 6 0.0 7 0.003981067649296429 8 1.0226595796357797E-4 9 1.0957066924669068E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.337507610596068E-4 32-33 0.0 34-35 3.652355641556356E-6 36-37 2.3009840541805045E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 1.5705129258692332E-4 50-51 0.0 52-53 0.0 54-55 1.8992249336093053E-4 56-57 0.0 58-59 2.1183662721026866E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.652355641556356E-6 68-69 0.0 70-71 7.304711283112713E-5 72-73 2.702743174751704E-4 74-75 9.605695337293217E-4 76-77 0.0020453191592715594 78-79 3.17754940815403E-4 80-81 4.0906383185431187E-4 82-83 4.346303213452064E-4 84-85 3.652355641556356E-5 86-87 6.501193041970315E-4 88-89 2.4836018362583225E-4 90-91 7.304711283112712E-6 92-93 0.0 94-95 0.0 96-97 0.0 98-99 3.652355641556356E-6 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3689795E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.127363936373577 #Duplication Level Percentage of deduplicated Percentage of total 1 64.07809599448252 12.256450624363492 2 12.527088153490354 4.792203483496881 3 6.0674338362603635 3.4816204543805775 4 3.590198091236199 2.746841019789941 5 2.418228955114031 2.3127172652971235 6 1.7366102507613181 1.9930065769169225 7 1.3382077068412 1.7917470081848086 8 1.0122722274607152 1.5489679437859707 9 0.8148468642194718 1.402728527190476 >10 5.62485559406347 20.75252541037115 >50 0.5031364943330423 6.631274639195539 >100 0.2551550833964732 8.84085838634006 >500 0.015458563512343789 2.0556619182007956 >1k 0.013809138621448546 5.596907284983954 >5k 0.002033012074824369 2.722933781389742 >10k+ 0.002570034132325146 21.073555676112594 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCA 243714 1.7802604056525315 No Hit CCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTA 232462 1.6980677942949476 No Hit GCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT 183703 1.3418973768416547 No Hit ATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGA 164337 1.200434338132894 No Hit CGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATT 144404 1.0548295281266082 No Hit CGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACC 110438 0.8067177046844018 No Hit CTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGGGCTCTC 79884 0.5835295561401759 No Hit CCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACT 74691 0.5455961904469717 No Hit GTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGC 70211 0.5128710838986267 No Hit GGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGC 62211 0.45443339363372504 No Hit CGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGGGCTCTCAC 60402 0.44121917092257407 No Hit CGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACA 60091 0.43894740571352603 No Hit CCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTAC 57791 0.42214656976236675 No Hit CCACGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTT 53135 0.388135834028194 No Hit GGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCT 51669 0.3774271272871508 No Hit CGGCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTG 51282 0.37460020402058614 No Hit ATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGC 50141 0.3662655284465545 No Hit CTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCG 48840 0.3567620990672249 No Hit CACGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTT 47177 0.3446143642034084 No Hit CCGGGCTCTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACC 44494 0.32501582383081706 No Hit GGCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTGA 41344 0.302005983289012 No Hit CCGCTATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGG 41251 0.3013266451396825 No Hit CGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTC 36939 0.2698287300869005 No Hit CGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCG 36402 0.26590610012786897 No Hit GAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTC 35815 0.2616182346046818 No Hit GGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCG 32695 0.23882753540137017 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 30301 0.22134005658959832 No Hit GAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCAC 28575 0.20873212491494575 No Hit CCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTAT 27651 0.20198257168934963 No Hit CTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGC 27441 0.20044858231989593 No Hit CGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGAT 27246 0.19902416361968897 No Hit CTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAA 26876 0.19632142044493728 No Hit GTCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT 26148 0.1910035906308312 No Hit GCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCG 24620 0.179841991790235 No Hit CTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAG 24501 0.17897273114754458 No Hit CCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGA 22500 0.16435600387003604 No Hit CCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT 22418 0.1637570175448208 No Hit GGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGATA 22245 0.1624933024928423 No Hit ACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTAC 21671 0.1583003982163356 No Hit GTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAG 21186 0.15475761324402595 No Hit GTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACACTTTCTG 21185 0.15475030853274283 No Hit CCGCTAACCACGACACTTTCTGACTGCCTCTCAGCCGGGTAGCGCTGAGA 20628 0.15068158434804904 No Hit CACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACACTT 20443 0.14933021276067318 No Hit CTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACAC 20433 0.14925716564784205 No Hit CCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATG 19449 0.14206932974525915 No Hit CTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGG 19155 0.139921744628024 No Hit CATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGG 18041 0.13178429625863644 No Hit TCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGAT 17287 0.12627654395116947 No Hit CCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCC 16707 0.1220398114069641 No Hit CGGGCTCTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCA 16528 0.12073226808728692 No Hit CCCAGTCGCTGTGGTTGTCCCGAGGAGTCCCGCGACCGGACTCATCGGTG 16192 0.11827788509616104 No Hit GCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTGAC 15360 0.11220036530861126 No Hit CTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGAT 15346 0.11209809935064768 No Hit CTCTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGAC 14637 0.10691905905092078 No Hit GCCACTTCCGCTAACCACGACACTTTCTGACTGCCTCTCAGCCGGGTAGC 14538 0.10619589263389262 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.4609422566225426E-5 2.191413384933814E-5 0.0 0.0 7.304711283112713E-6 2 1.4609422566225426E-5 3.652355641556356E-5 0.0 0.0 2.9218845132450852E-5 3 2.9218845132450852E-5 1.6070364822847968E-4 0.0 0.0 7.304711283112713E-5 4 3.652355641556356E-5 1.8992249336093053E-4 0.0 0.0 1.0226595796357798E-4 5 8.765653539735255E-5 1.8992249336093053E-4 0.0 0.0 1.095706692466907E-4 6 1.3148480309602882E-4 2.702743174751704E-4 0.0 0.0 1.095706692466907E-4 7 1.3878951437914155E-4 2.775790287582831E-4 0.0 0.0 1.095706692466907E-4 8 1.5339893694536698E-4 3.0679787389073396E-4 0.0 0.0 1.095706692466907E-4 9 1.8992249336093053E-4 3.0679787389073396E-4 7.304711283112713E-6 0.0 1.3148480309602882E-4 10-11 2.3009840541805045E-4 3.2140729645695936E-4 7.304711283112713E-6 0.0 1.4609422566225425E-4 12-13 2.9584080696606484E-4 3.2505965209851575E-4 1.826177820778178E-5 0.0 1.4609422566225425E-4 14-15 4.0906383185431187E-4 3.287120077400721E-4 4.017591205711992E-5 0.0 1.6070364822847968E-4 16-17 5.62462768799679E-4 3.360167190231848E-4 5.113297898178899E-5 0.0 2.848837400413958E-4 18-19 7.925611742177293E-4 3.7984498672186107E-4 5.8437690264901705E-5 0.0 7.706470403683912E-4 20-21 9.824836675786598E-4 4.6750152211921364E-4 6.209004590645806E-5 0.0 9.53264822446209E-4 22-23 0.001271019763261612 4.748062334023263E-4 8.765653539735255E-5 0.0 9.678742450124345E-4 24-25 0.0016252982604925787 4.748062334023263E-4 2.191413384933814E-4 0.0 9.788313119371036E-4 26-27 0.0020854950713286797 4.748062334023263E-4 2.3375076105960682E-4 0.0 0.001004397801427998 28-29 0.0027319620198841543 4.8211094468543904E-4 2.593172505505013E-4 0.0 0.001073792558617569 30-31 0.003652355641556356 4.8211094468543904E-4 2.6296960619205765E-4 0.0 0.0011212731819578014 32-33 0.005274001546407378 4.8211094468543904E-4 2.6296960619205765E-4 0.0 0.001260062696336943 34-35 0.007805084006005934 4.930680116101081E-4 2.739266731167267E-4 0.0 0.00149381345739655 36-37 0.01036538531073694 5.1132978981789E-4 2.775790287582831E-4 0.0 0.001548598792019895 38-39 0.01338223107066249 5.222868567425589E-4 2.8123138439983944E-4 0.0 0.001650864749983473 40-41 0.018290997052914232 5.368962793087845E-4 2.848837400413958E-4 0.0 0.001906529644892418 42-43 0.024993069655170147 5.624627687996789E-4 2.9584080696606484E-4 0.0 0.0020562762261962285 44-45 0.03182297470488053 5.807245470074607E-4 3.104502295322903E-4 0.0 0.0020964521382533484 46-47 0.039419874439317756 6.099433921399115E-4 3.2505965209851575E-4 0.0 0.0021147139164611303 48-49 0.048002910196975196 6.35509881630806E-4 3.3966907466474115E-4 0.0 0.002286374631614279 50-51 0.057418683040907484 6.35509881630806E-4 3.433214303062975E-4 0.0 0.0023849882339363007 52-53 0.06923040118570073 6.391622372723623E-4 3.469737859478539E-4 0.0 0.0024434259242012025 54-55 0.08465429905999322 6.428145929139187E-4 3.5427849723096655E-4 0.0 0.0024726447693336536 56-57 0.10432953890105731 6.501193041970314E-4 3.5793085287252294E-4 0.0 0.0025274301039569986 58-59 0.12863596569561486 6.720334380463696E-4 3.5793085287252294E-4 0.0 0.0025529965934478933 60-61 0.156675099955843 7.304711283112712E-4 3.5793085287252294E-4 0.0 0.0025676060160141185 62-63 0.19471438396265245 9.276983329553145E-4 3.68887919797192E-4 0.0 0.002600477216788126 64-65 0.23563172421500833 0.0010628354916928998 3.7254027543874834E-4 0.0 0.0026370007732036895 66-67 0.2798288798334818 0.0010993590481084632 3.761926310803047E-4 0.0 0.0026990908191101472 68-69 0.32875583600777075 0.0011358826045240267 3.7984498672186107E-4 0.0 0.0027648332206581614 70-71 0.39009349665206816 0.0012856291858278374 3.7984498672186107E-4 0.0 0.0028963180237541906 72-73 0.46959066954618384 0.0018006113312872838 3.7984498672186107E-4 0.0 0.0029547557140190924 74-75 0.5695483387442982 0.002008795602855996 3.7984498672186107E-4 0.0 0.0029693651365853176 76-77 0.6813432925766967 0.002027057381063778 3.7984498672186107E-4 0.0 0.0030058886930008815 78-79 0.8122181522805856 0.0020307097367053342 3.7984498672186107E-4 0.0 0.003086240517115121 80-81 0.9616944592669211 0.0020307097367053342 3.871496980049738E-4 0.0 0.0031885064750786993 82-83 1.1373508514919326 0.002038014447988447 3.871496980049738E-4 0.0 0.003360167190231848 84-85 1.3429675170446307 0.002078190360045567 3.871496980049738E-4 0.0 0.003473390215120095 86-87 1.574581650053927 0.002147585117235138 3.871496980049738E-4 0.0 0.0035099137715356586 88-89 1.8569416123470073 0.002202370451858483 3.871496980049738E-4 0.0 0.0035610467505174477 90 2.0935959961416515 0.002220632230066265 3.871496980049738E-4 0.0 0.0036231367964239054 >>END_MODULE