FastQCFastQC Report
Thu 25 Jan 2024
SRR25792494_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR25792494_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13689795
Sequences flagged as poor quality0
Sequence length101
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCA2437141.7802604056525315No Hit
CCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTA2324621.6980677942949476No Hit
GCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT1837031.3418973768416547No Hit
ATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGA1643371.200434338132894No Hit
CGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATT1444041.0548295281266082No Hit
CGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACC1104380.8067177046844018No Hit
CTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGGGCTCTC798840.5835295561401759No Hit
CCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACT746910.5455961904469717No Hit
GTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGC702110.5128710838986267No Hit
GGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGC622110.45443339363372504No Hit
CGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGGGCTCTCAC604020.44121917092257407No Hit
CGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACA600910.43894740571352603No Hit
CCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTAC577910.42214656976236675No Hit
CCACGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTT531350.388135834028194No Hit
GGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCT516690.3774271272871508No Hit
CGGCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTG512820.37460020402058614No Hit
ATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGC501410.3662655284465545No Hit
CTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCG488400.3567620990672249No Hit
CACGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTT471770.3446143642034084No Hit
CCGGGCTCTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACC444940.32501582383081706No Hit
GGCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTGA413440.302005983289012No Hit
CCGCTATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGG412510.3013266451396825No Hit
CGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTC369390.2698287300869005No Hit
CGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCG364020.26590610012786897No Hit
GAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTC358150.2616182346046818No Hit
GGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCG326950.23882753540137017No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA303010.22134005658959832No Hit
GAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCAC285750.20873212491494575No Hit
CCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTAT276510.20198257168934963No Hit
CTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGC274410.20044858231989593No Hit
CGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGAT272460.19902416361968897No Hit
CTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAA268760.19632142044493728No Hit
GTCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT261480.1910035906308312No Hit
GCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCG246200.179841991790235No Hit
CTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAG245010.17897273114754458No Hit
CCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGA225000.16435600387003604No Hit
CCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT224180.1637570175448208No Hit
GGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGATA222450.1624933024928423No Hit
ACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTAC216710.1583003982163356No Hit
GTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAG211860.15475761324402595No Hit
GTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACACTTTCTG211850.15475030853274283No Hit
CCGCTAACCACGACACTTTCTGACTGCCTCTCAGCCGGGTAGCGCTGAGA206280.15068158434804904No Hit
CACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACACTT204430.14933021276067318No Hit
CTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACAC204330.14925716564784205No Hit
CCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATG194490.14206932974525915No Hit
CTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGG191550.139921744628024No Hit
CATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGG180410.13178429625863644No Hit
TCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGAT172870.12627654395116947No Hit
CCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCC167070.1220398114069641No Hit
CGGGCTCTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCA165280.12073226808728692No Hit
CCCAGTCGCTGTGGTTGTCCCGAGGAGTCCCGCGACCGGACTCATCGGTG161920.11827788509616104No Hit
GCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTGAC153600.11220036530861126No Hit
CTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGAT153460.11209809935064768No Hit
CTCTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGAC146370.10691905905092078No Hit
GCCACTTCCGCTAACCACGACACTTTCTGACTGCCTCTCAGCCGGGTAGC145380.10619589263389262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)