FastQCFastQC Report
Thu 25 Jan 2024
SRR25792493_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR25792493_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13904011
Sequences flagged as poor quality0
Sequence length101
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCC2275391.636498992988426No Hit
GGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCG1867991.343490018815434No Hit
GTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAG1487761.070022168423198No Hit
GTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGA1303060.9371828028617066No Hit
TATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTA998210.7179295240776205No Hit
GGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTC982990.7069830425191694No Hit
GTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAG967710.6959934079453763No Hit
CAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGAC918320.6604712841495882No Hit
GGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGAT895360.6439580636120038No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA894480.6433251527203193No Hit
GGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTC885080.636564513650054No Hit
GGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCT804210.5784014411381003No Hit
CGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGA753160.5416854172511802No Hit
CTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAG717040.5157073020152243No Hit
GTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCG614860.44221771688759454No Hit
GGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTG605230.43529165792518437No Hit
CGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCG577360.4152470822987697No Hit
CATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGT574620.4132764279314797No Hit
GTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAG543670.3910166641841696No Hit
GTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAA536530.38588145535845736No Hit
GGCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTT536170.3856225372664046No Hit
CGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTA529110.3805448657944819No Hit
GTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCG519420.3735756538167296No Hit
GCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTAGC515170.37051898189666277No Hit
CTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGG505200.3633483891806472No Hit
GTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCT500690.36010472086076456No Hit
CTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGA495890.35665247963339497No Hit
CCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGG495510.3563791772028949No Hit
GGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGG480730.3457491510902861No Hit
CCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGG471650.33921866143517865No Hit
GATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGT462350.33252994405715014No Hit
GGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACC453260.325992262232819No Hit
GTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAG452780.32564703811008205No Hit
CGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCC436480.3139238022754729No Hit
AGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTG425650.3061346830062203No Hit
AGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACG414780.2983167950600729No Hit
CTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGC414100.29782772755286224No Hit
GAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGT395970.28478832475031846No Hit
TGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCT386360.2778766501263556No Hit
GTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGT380580.27371957631506477No Hit
GGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCAC374910.2696416163652345No Hit
ATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTT368260.26485882383148285No Hit
GGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCC366650.2637008845864693No Hit
CCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGG366070.2632837387714955No Hit
GGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACC365520.26288816946419274No Hit
GAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGG364250.2619747639727845No Hit
GCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCG363370.26134185308110014No Hit
CCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCT362270.2605507144664946No Hit
GAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTA340330.2447710951897262No Hit
CGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTAGCAGCGGGCCCG316370.22753865772977308No Hit
CGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATAT313660.22558957987015402No Hit
GAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTG308910.22217329948890288No Hit
CAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGT297380.21388072837399222No Hit
GGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAA296650.21335570002066312No Hit
GCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATG287840.2070193989345952No Hit
GCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTG271250.19508759019249913No Hit
GTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTG268430.1930593984714195No Hit
TGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGT252210.18139369999059984No Hit
GTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAG240670.17309393670646547No Hit
AGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGC230980.16612472472871317No Hit
CGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCC218490.1571417053683286No Hit
GGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGG218050.1568252499224864No Hit
CAGTTGTCCCGCCAGGGGCACCGCTGGATAGCCACGTTCGGTCAGGATAA215900.1552789335393938No Hit
CTTCTCCAAGATCAGGTTTCTCACCCACTTGGTGGGATAAGGCCCCCCGC215450.1549552859243279No Hit
GAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTC208550.14999268915998412No Hit
GGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCC208340.1498416536062867No Hit
AAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGT208170.14971938672948404No Hit
GCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCG193710.1393195100320332No Hit
GTTGTCCCGCCAGGGGCACCGCTGGATAGCCACGTTCGGTCAGGATAACC192860.13870817564801985No Hit
CCTGCCTAGTATCAATTCCCGAGTAGCAGCGGGCCCGTGGAATCCGCTGT184530.1327170986846889No Hit
GCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGC182780.13145846907054373No Hit
GGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGA173090.12448925709279142No Hit
GTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCC170910.12292136420202775No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA169930.12221653161810644No Hit
GTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAG166980.12009484169711893No Hit
ATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTC163630.1176854650071839No Hit
GGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTAC162950.11719639749997321No Hit
CGTGTGTGGGCGCCCGTGACGAATCAGCGGTACTAACCACCCAAAACCGG161790.11636210587002557No Hit
TGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGC150540.10827091549337814No Hit
CGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATC146370.10527178092710082No Hit
CGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTAGCAGC146250.10518547489641657No Hit
GGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGA143430.10315728317533697No Hit
CCTTCTCCAAGATCAGGTTTCTCACCCACTTGGTGGGATAAGGCCCCCCG140390.10097086373133622No Hit
GTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAAC139960.1006616004547177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)