FastQCFastQC Report
Thu 25 Jan 2024
SRR25792493_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR25792493_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13904011
Sequences flagged as poor quality0
Sequence length101
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCA4007122.881988513961906No Hit
ATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGA3856392.773580947253278No Hit
CGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACA3102582.2314280390025583No Hit
GTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGC2480541.784046344612357No Hit
CGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATT1975361.420712339770157No Hit
CCGCTATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGG1956401.4070759869220473No Hit
GCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT1802521.2964028869079576No Hit
CCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTA1127510.8109242721398883No Hit
CTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGGGCTCTC938760.6751720780428037No Hit
CGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGGGCTCTCAC898380.6461300987175571No Hit
GGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCT833760.5996543011940944No Hit
CGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACC769670.5535596886394868No Hit
GAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTC746440.5368522795328629No Hit
GGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGC743990.5350901980730597No Hit
ATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGC719390.5173974617827906No Hit
CCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACT596010.42866047790094525No Hit
CATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGG526010.3783152933351391No Hit
GAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCAC521210.3748630521077695No Hit
ATCCGCTATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGC505330.3634418873805551No Hit
ATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCA505260.36339154219598935No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470270.3382261420823099No Hit
GGCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTGA464080.3337741893328479No Hit
CCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTAC432250.31088151469385344No Hit
GGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCG389920.28043706236998805No Hit
CCACGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTT388370.2793222761403166No Hit
CCGGGCTCTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACC359380.25847217756084917No Hit
CACGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTT356930.25671009610104595No Hit
CGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTC354110.2546819043799663No Hit
CGGCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTG343190.24682805558770057No Hit
CTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCG342170.24609445432688454No Hit
ACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTAC338850.2437066541446206No Hit
GGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAG328190.23603980175217065No Hit
CGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCG299460.21537669957251904No Hit
GCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCG266800.1918870748879586No Hit
CGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGAT236140.16983588404813546No Hit
CTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAA228620.16442737279192313No Hit
GGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGATA226500.1629026329165016No Hit
GTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAG222380.1599394591963427No Hit
TCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGAT218180.1569187481223943No Hit
CTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGC214270.15410660995593287No Hit
CGCTATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGA209720.15083417295915547No Hit
TCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCG209540.1507047139131291No Hit
CCCGTGTTCTGCGGGGGGCCTTATCCCACCAAGTGGGTGAGAAACCTGAT203450.14632468285590397No Hit
CTATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATT202120.14536812434915364No Hit
CTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAG200940.14451944838075861No Hit
GTCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT200010.14385057664295578No Hit
GCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTG199400.1434118543203109No Hit
CCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTAT191060.13741358518775626No Hit
CCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGA185260.13324212703801802No Hit
GTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAAT183170.13173896367026752No Hit
TATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTC179160.1288549038115692No Hit
CCGCTAACCACGACACTTTCTGACTGCCTCTCAGCCGGGTAGCGCTGAGA171650.12345358472458055No Hit
GCCGGGTTTTCGCGTACCGGGCTCTCACCGTCTACGGCAGACCATCCCAG163380.11750566077659173No Hit
GTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACACTTTCTG163240.11740497040746013No Hit
GCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTGAC162670.11699501676170998No Hit
CTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACAC157940.11359312071890623No Hit
CACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACACTT150040.1079113070321938No Hit
CCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT148970.10714174492525932No Hit
CTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGG144980.1042720694050084No Hit
AGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCC144310.10379019406702138No Hit
GGCAGGTGCCGGGTTTTCGCGTACCGGGCTCTCACCGTCTACGGCAGACC141850.10202092043799449No Hit
GTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGG139490.10032356850120444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)