FastQCFastQC Report
Thu 25 Jan 2024
SRR25792492_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR25792492_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15396871
Sequences flagged as poor quality0
Sequence length101
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCG1836001.1924500763824026No Hit
GGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTC1778751.1552671968220036No Hit
GTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGA1685281.094560057040161No Hit
GGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCC1384530.8992281613582396No Hit
GGCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTT1358400.8822571806959998No Hit
TATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTA1250320.8120610999468657No Hit
GTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAG1105680.7181199348880691No Hit
CGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTA1083480.7037014208925957No Hit
GGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCT1073760.6973884498999829No Hit
CAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGAC1043430.6776896422656266No Hit
CATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGT1035140.6723054314087583No Hit
CGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATAT950600.6173981713557254No Hit
CTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGA851350.5529370220741604No Hit
GGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTG767940.4987636773731494No Hit
GGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTC754410.4899761776272595No Hit
GTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAG748990.4864559818680042No Hit
GGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACC743650.48298774471774164No Hit
GCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTG736440.4783049750822749No Hit
TGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCT736160.4781231199508004No Hit
GTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAG718430.46660779323279383No Hit
CCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCT679370.4412390023921094No Hit
GTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAG598760.3888842090058428No Hit
CGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGA590440.3834805136706023No Hit
GTTTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGA589790.3830583499725366No Hit
GTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCG570330.37041941833506303No Hit
GGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGAT569390.3698089046793988No Hit
CTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGG546100.35468245463639986No Hit
GGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGG495180.321610800012548No Hit
GATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGT474020.3078677479339796No Hit
CGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCG462500.30038570823903116No Hit
AGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTG425820.27656268601587947No Hit
GTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCC425510.27636134640603277No Hit
CTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAG415920.2701328081530332No Hit
CAGGCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGG409390.26589168669400426No Hit
GTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAA406460.2639887026396467No Hit
ATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTT397260.2580134626054865No Hit
AGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACG395850.25709769212199024No Hit
CCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGG369430.23993836150215195No Hit
GTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGT346210.22485737524202157No Hit
ATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTC344430.22370129619193407No Hit
GAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGT342860.22268160849045238No Hit
CTTCTCCAAGATCAGGTTTCTCACCCACTTGGTGGGATAAGGCCCCCCGC332790.21614131858349658No Hit
GGTGAGGCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGG324750.21091947837973052No Hit
CTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGC310750.2018267218060085No Hit
GAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGG309710.20115125988910346No Hit
CCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGG305630.1985013708304759No Hit
GGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTAC287200.18653140628378326No Hit
GTTGTCCCGCCAGGGGCACCGCTGGATAGCCACGTTCGGTCAGGATAACC279020.18121863851427994No Hit
GTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTG270590.17574350009167447No Hit
CCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGG270490.1756785518304336No Hit
CAGTTGTCCCGCCAGGGGCACCGCTGGATAGCCACGTTCGGTCAGGATAA266660.17319103342490821No Hit
CGTGTGTGGGCGCCCGTGACGAATCAGCGGTACTAACCACCCAAAACCGG265420.1723856749855214No Hit
TGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGT251600.16340982528203296No Hit
GTGAGGCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGG246880.16034426735146382No Hit
GTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAG241810.15705139050655162No Hit
GGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACC241260.15669417506972683No Hit
CTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGT234780.15248552774131835No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA229690.14917966124415796No Hit
GTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCG222890.14476317947977874No Hit
CGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGG218330.14180153876719498No Hit
GCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATG217510.14126896302501984No Hit
AACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCG216870.14085329415307823No Hit
CAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGT200280.13007837761321767No Hit
TTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGT198510.12892879338925423No Hit
AGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGG195680.12709075759613755No Hit
GGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCAC195090.1267075628548164No Hit
GTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCT190030.12342118083602831No Hit
GGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAA188980.12273922409299916No Hit
AGGCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGT186210.12094015725662702No Hit
AAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGT185000.12015428329561248No Hit
GCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCG183820.11938789381297017No Hit
GCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATG182370.11844614402497754No Hit
GTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAG182050.11823830958900676No Hit
CGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCC181340.11777717693419655No Hit
GGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGC176850.11486100000448143No Hit
CCTTCTCCAAGATCAGGTTTCTCACCCACTTGGTGGGATAAGGCCCCCCG176280.11449079491540846No Hit
ACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGC174470.1133152313869487No Hit
GAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTG174000.1130099745591166No Hit
CTCGGCCCGGACCCTGGCATCAGCTAGAGGGATCCACCGATGAGTCCGGT172430.1119902868576349No Hit
TGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGA170620.11081472332917512No Hit
CTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACC169840.11030812689149633No Hit
TTTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAG169260.10993142697629926No Hit
ATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTG169210.10989895284567884No Hit
GTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGG165750.10765174300674468No Hit
TGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGC165530.10750885683201475No Hit
ATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAG163580.10624236573781778No Hit
ACGGTTTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCA162560.10557989347316087No Hit
CCTCGGCCCGGACCCTGGCATCAGCTAGAGGGATCCACCGATGAGTCCGG160450.10420948516097847No Hit
GGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGG159310.10346907498283255No Hit
CCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAG158380.10286505615329244No Hit
AGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGC154940.10063083596660645No Hit
GCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTAGC154710.10048145496575245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)