##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25787977_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12627739
Sequences flagged as poor quality	0
Sequence length	101
%GC	62
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.324142350423934	35.0	33.0	35.0	31.0	35.0
2	33.396859326915134	35.0	33.0	35.0	31.0	35.0
3	33.503575184757935	35.0	33.0	35.0	31.0	35.0
4	33.61958581817378	35.0	34.0	35.0	31.0	35.0
5	33.44387811626452	35.0	33.0	35.0	31.0	35.0
6	37.89060306045287	40.0	39.0	40.0	34.0	40.0
7	37.969683963217804	40.0	39.0	40.0	34.0	40.0
8	38.019967945172134	40.0	39.0	40.0	34.0	40.0
9	38.11283088761971	40.0	39.0	40.0	34.0	40.0
10-11	38.13767654684659	40.0	39.0	40.0	34.5	40.0
12-13	38.1331254550003	40.0	39.0	40.0	34.0	40.0
14-15	38.137356537064946	40.0	39.0	40.0	34.0	40.0
16-17	38.111766286902196	40.0	39.0	40.0	34.0	40.0
18-19	38.10728797134625	40.0	39.0	40.0	34.0	40.0
20-21	38.13137335987068	40.0	39.0	40.0	34.0	40.0
22-23	38.09331880394424	40.0	39.0	40.0	34.0	40.0
24-25	38.08021835896355	40.0	39.0	40.0	34.0	40.0
26-27	38.089118408291455	40.0	39.0	40.0	34.0	40.0
28-29	38.069957773121544	40.0	39.0	40.0	34.0	40.0
30-31	38.079167339457996	40.0	39.0	40.0	34.0	40.0
32-33	38.0607046518779	40.0	39.0	40.0	34.0	40.0
34-35	38.04552727135079	40.0	39.0	40.0	34.0	40.0
36-37	38.020350436447885	40.0	39.0	40.0	34.0	40.0
38-39	38.039496579712335	40.0	39.0	40.0	34.0	40.0
40-41	37.994481157711604	40.0	39.0	40.0	34.0	40.0
42-43	37.93417226155846	40.0	39.0	40.0	34.0	40.0
44-45	37.87940263890472	40.0	39.0	40.0	34.0	40.0
46-47	37.845952866146504	40.0	39.0	40.0	34.0	40.0
48-49	37.83400595308471	40.0	39.0	40.0	34.0	40.0
50-51	37.82823591776801	40.0	39.0	40.0	34.0	40.0
52-53	37.806798548813845	40.0	39.0	40.0	34.0	40.0
54-55	37.80428634136325	40.0	39.0	40.0	34.0	40.0
56-57	37.81420122002838	40.0	39.0	40.0	34.0	40.0
58-59	37.79307320970128	40.0	39.0	40.0	34.0	40.0
60-61	37.75343357191656	40.0	39.0	40.0	34.0	40.0
62-63	37.72809788038856	40.0	39.0	40.0	34.0	40.0
64-65	37.703289559595746	40.0	39.0	40.0	34.0	40.0
66-67	37.672197295176915	40.0	38.5	40.0	34.0	40.0
68-69	37.65035672656839	40.0	38.0	40.0	34.0	40.0
70-71	37.64685039815916	40.0	38.0	40.0	34.0	40.0
72-73	37.61576625079121	40.0	38.0	40.0	34.0	40.0
74-75	37.59397161281208	40.0	38.0	40.0	34.0	40.0
76-77	37.596521079506	40.0	38.0	40.0	34.0	40.0
78-79	37.53866523532044	39.0	38.0	40.0	34.0	40.0
80-81	37.47559709620226	39.0	38.0	40.0	34.0	40.0
82-83	37.40979434243929	39.0	38.0	40.0	34.0	40.0
84-85	37.412432621548476	39.0	38.0	40.0	34.0	40.0
86-87	37.414147180267186	39.0	38.0	40.0	34.0	40.0
88-89	37.37407512144494	39.0	38.0	40.0	34.0	40.0
90-91	37.32179497849932	39.0	38.0	40.0	34.0	40.0
92-93	37.278967953012014	39.0	38.0	40.0	34.0	40.0
94-95	37.22589665497521	39.0	38.0	40.0	31.0	40.0
96-97	37.169907296943656	39.0	38.0	40.0	31.0	40.0
98-99	37.09393593738356	39.0	37.5	40.0	31.0	40.0
100-101	35.6382134204706	38.0	35.5	39.5	23.5	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.010759431286906818
1101	2	0.1253040568368391
1101	3	0.12593416312483896
1101	4	0.1848220483110623
1101	5	0.15443027048301872
1101	6	0.36704360012987536
1101	7	0.44253624523985735
1101	8	0.4024707599905355
1101	9	0.4137985520201326
1101	10-11	0.47856114244866177
1101	12-13	0.45659222436025004
1101	14-15	0.4488143108077125
1101	16-17	0.4640110499193213
1101	18-19	0.45453437398573016
1101	20-21	0.42732850208529527
1101	22-23	0.46390527216007627
1101	24-25	0.44663117051223367
1101	26-27	0.45482730351098866
1101	28-29	0.5054174566773355
1101	30-31	0.5245098002582509
1101	32-33	0.4880153795416504
1101	34-35	0.4030733899419374
1101	36-37	0.47210918111027667
1101	38-39	0.43130937183393314
1101	40-41	0.4745474855810201
1101	42-43	0.4573240711240203
1101	44-45	0.39497758189445165
1101	46-47	0.4651563975793849
1101	48-49	0.45051578244346047
1101	50-51	0.42550194626982574
1101	52-53	0.4694601791150035
1101	54-55	0.42588057325524176
1101	56-57	0.396516155467026
1101	58-59	0.42400052041733005
1101	60-61	0.44432565231267773
1101	62-63	0.40712563879736763
1101	64-65	0.36829351333853566
1101	66-67	0.430775475557013
1101	68-69	0.4522367105248435
1101	70-71	0.4215742279541388
1101	72-73	0.4097465498846731
1101	74-75	0.3732943248226732
1101	76-77	0.39049355248389617
1101	78-79	0.41661683756874623
1101	80-81	0.4261120872280273
1101	82-83	0.4674013936439323
1101	84-85	0.5046621864270193
1101	86-87	0.43661688260201004
1101	88-89	0.4757854785848963
1101	90-91	0.42478562181038626
1101	92-93	0.4913264310760965
1101	94-95	0.47653846289883717
1101	96-97	0.4190306332053808
1101	98-99	0.4111703774768216
1101	100-101	0.43872970266581035
1102	1	0.044048812380310665
1102	2	0.09263288020259353
1102	3	0.19189759928300987
1102	4	0.2048912531613638
1102	5	0.1467292756457681
1102	6	0.2504566467170761
1102	7	0.3073000450446699
1102	8	0.28131611252212707
1102	9	0.38695601267463076
1102	10-11	0.3845408751979491
1102	12-13	0.4186067884314184
1102	14-15	0.4279380506240642
1102	16-17	0.42806609217799974
1102	18-19	0.3800724793501118
1102	20-21	0.4059780973545912
1102	22-23	0.3585976055784741
1102	24-25	0.4253508690957801
1102	26-27	0.4547733513902159
1102	28-29	0.4130743610935923
1102	30-31	0.4317752288980117
1102	32-33	0.4053305961727034
1102	34-35	0.36790578373012295
1102	36-37	0.37142999697754675
1102	38-39	0.4278631339905985
1102	40-41	0.42388331664579226
1102	42-43	0.42871338817602833
1102	44-45	0.38228415843655483
1102	46-47	0.3945386530540631
1102	48-49	0.3779289700245556
1102	50-51	0.41549268387404226
1102	52-53	0.38636594515504186
1102	54-55	0.4410528082047449
1102	56-57	0.3923123460782705
1102	58-59	0.34669889416066724
1102	60-61	0.34778790849884444
1102	62-63	0.3772960450824314
1102	64-65	0.4365276375456659
1102	66-67	0.3900827158110687
1102	68-69	0.3166372899160024
1102	70-71	0.2741509774291515
1102	72-73	0.3252226031755896
1102	74-75	0.3334394405283163
1102	76-77	0.32257651999618275
1102	78-79	0.35745618753943376
1102	80-81	0.32580205258377504
1102	82-83	0.3342823252250895
1102	84-85	0.4217420606105762
1102	86-87	0.36814663483482946
1102	88-89	0.4052215754714865
1102	90-91	0.3895003474500811
1102	92-93	0.3804402216096605
1102	94-95	0.33742026246211054
1102	96-97	0.33789245577198557
1102	98-99	0.4022074768543362
1102	100-101	0.3747948527177769
1103	1	0.06658964886622698
1103	2	0.0766474122453289
1103	3	0.2185514701616853
1103	4	0.21662152661971135
1103	5	0.13452653723355468
1103	6	0.28077949956085035
1103	7	0.24496641258475194
1103	8	0.3049930512942325
1103	9	0.4235851141752036
1103	10-11	0.3615098908817629
1103	12-13	0.3943444316955862
1103	14-15	0.35665312105034985
1103	16-17	0.38438880556734745
1103	18-19	0.3901859415346749
1103	20-21	0.42686541726919813
1103	22-23	0.461027299847224
1103	24-25	0.43452746817883536
1103	26-27	0.4113997914059553
1103	28-29	0.370933090681433
1103	30-31	0.4264785169666325
1103	32-33	0.38413129673656954
1103	34-35	0.3964278040523226
1103	36-37	0.412725032537395
1103	38-39	0.39511335251361857
1103	40-41	0.4486707557110563
1103	42-43	0.3925850844031302
1103	44-45	0.3575852002136273
1103	46-47	0.36388304371514124
1103	48-49	0.3707863477503679
1103	50-51	0.36440696176128995
1103	52-53	0.3961423161545454
1103	54-55	0.3814483112784046
1103	56-57	0.3160501291276887
1103	58-59	0.3268560321785756
1103	60-61	0.3360496790014693
1103	62-63	0.34080246356337085
1103	64-65	0.36461829847561233
1103	66-67	0.4069417795968633
1103	68-69	0.3400801362335528
1103	70-71	0.28398111335244636
1103	72-73	0.3323704772921303
1103	74-75	0.34452575334042734
1103	76-77	0.34355536611277415
1103	78-79	0.3529526677387338
1103	80-81	0.3687959247471113
1103	82-83	0.3654115928498811
1103	84-85	0.42956804859451125
1103	86-87	0.35230709159175433
1103	88-89	0.38307568314267826
1103	90-91	0.4213074128865202
1103	92-93	0.38114062922292646
1103	94-95	0.3315418764984699
1103	96-97	0.3678071875920921
1103	98-99	0.300078205395522
1103	100-101	0.4147448435997205
1104	1	-0.0067829981129534644
1104	2	0.03279998946359797
1104	3	0.04268863559672553
1104	4	0.15242542832228168
1104	5	0.0637090784284382
1104	6	0.1460272703101566
1104	7	0.20684126816343706
1104	8	0.25572847904549434
1104	9	0.31236236936910444
1104	10-11	0.2479411146206374
1104	12-13	0.32657705465400966
1104	14-15	0.3151625001029643
1104	16-17	0.30431842029530287
1104	18-19	0.2892904783697716
1104	20-21	0.2756036903792918
1104	22-23	0.28977390937706815
1104	24-25	0.2709219309716815
1104	26-27	0.30618868053199577
1104	28-29	0.31063429433259415
1104	30-31	0.3067156342874213
1104	32-33	0.33810735792712165
1104	34-35	0.33713916219811324
1104	36-37	0.29619782532536476
1104	38-39	0.31886565367739195
1104	40-41	0.34161341856805194
1104	42-43	0.31164738707324346
1104	44-45	0.3235493540865306
1104	46-47	0.2781459422548025
1104	48-49	0.28038164580074465
1104	50-51	0.3039835654461811
1104	52-53	0.3015131619845235
1104	54-55	0.2922058181386262
1104	56-57	0.31073344886191734
1104	58-59	0.2578803770976563
1104	60-61	0.25332716792553356
1104	62-63	0.2772238070531756
1104	64-65	0.30128984951304716
1104	66-67	0.3083084994159435
1104	68-69	0.2743216471383363
1104	70-71	0.2488908591758232
1104	72-73	0.23575244975589982
1104	74-75	0.2672748605639512
1104	76-77	0.27696776579752935
1104	78-79	0.24398303878623295
1104	80-81	0.262457310868065
1104	82-83	0.3315527454838474
1104	84-85	0.35655143123146615
1104	86-87	0.3283017247387221
1104	88-89	0.2956780109510646
1104	90-91	0.2883945772346834
1104	92-93	0.2828916860368835
1104	94-95	0.2886109421685745
1104	96-97	0.31243541959052834
1104	98-99	0.2728310681985988
1104	100-101	0.27009392887887884
1105	1	-0.003049658958119039
1105	2	0.0117663888955164
1105	3	0.08872640138952903
1105	4	0.0989423528862261
1105	5	0.001614844049171893
1105	6	0.11818115839970744
1105	7	0.15492803766929342
1105	8	0.15125769743618633
1105	9	0.2096053569549099
1105	10-11	0.20798072808863566
1105	12-13	0.20620877755983713
1105	14-15	0.1872314770193455
1105	16-17	0.1550320042953075
1105	18-19	0.2047460533957448
1105	20-21	0.23355502970370168
1105	22-23	0.2346069824903978
1105	24-25	0.20319482641802722
1105	26-27	0.21484232917215706
1105	28-29	0.2397881326371305
1105	30-31	0.2199855245622686
1105	32-33	0.14563849300876086
1105	34-35	0.19882064397213384
1105	36-37	0.26993138590212595
1105	38-39	0.23771468358720682
1105	40-41	0.21932643344602099
1105	42-43	0.19883984426274992
1105	44-45	0.12624121140631672
1105	46-47	0.16490862764705128
1105	48-49	0.17530935073548903
1105	50-51	0.11840392527518162
1105	52-53	0.24638977012976682
1105	54-55	0.2360330893958107
1105	56-57	0.16160132183152598
1105	58-59	0.16075456962742152
1105	60-61	0.17192592981920285
1105	62-63	0.12599197219229552
1105	64-65	0.19298423832685074
1105	66-67	0.17344963435449046
1105	68-69	0.1674189845187044
1105	70-71	0.14544275733866385
1105	72-73	0.15291837756878124
1105	74-75	0.23997155736562803
1105	76-77	0.20320358055909793
1105	78-79	0.1303224011202957
1105	80-81	0.1446085730504052
1105	82-83	0.15672634145717979
1105	84-85	0.21065353082703098
1105	86-87	0.18010768646128383
1105	88-89	0.22740836358497774
1105	90-91	0.18517712323919966
1105	92-93	0.2711847643766774
1105	94-95	0.23674954599985654
1105	96-97	0.15357784199927949
1105	98-99	0.2008153265498791
1105	100-101	0.2416736052337214
1106	1	0.012988359439830788
1106	2	0.06807786317575193
1106	3	0.1016091285686116
1106	4	0.06551127416525304
1106	5	-0.02589219147086652
1106	6	0.06265926478340589
1106	7	0.10768661726668682
1106	8	0.10301997927671636
1106	9	0.14162168686032572
1106	10-11	0.17683707798043002
1106	12-13	0.10633815524951729
1106	14-15	0.20899480620578714
1106	16-17	0.2037580553751681
1106	18-19	0.16095140255540485
1106	20-21	0.1478842421521378
1106	22-23	0.16671571690213938
1106	24-25	0.19341729156674603
1106	26-27	0.16189592826573573
1106	28-29	0.17862730349011002
1106	30-31	0.19623667925001342
1106	32-33	0.15725910007913058
1106	34-35	0.16111931569790272
1106	36-37	0.19925501098825293
1106	38-39	0.18510862354422386
1106	40-41	0.21331502692512316
1106	42-43	0.17447067178624565
1106	44-45	0.13370886380244684
1106	46-47	0.17776922171596965
1106	48-49	0.06865757232081648
1106	50-51	0.1181210265188426
1106	52-53	0.17128791902475626
1106	54-55	0.18120922808522266
1106	56-57	0.18612177645683659
1106	58-59	0.1565851670803795
1106	60-61	0.1918027767143471
1106	62-63	0.1574280628405731
1106	64-65	0.15135011994587444
1106	66-67	0.16079758865606664
1106	68-69	0.1345343972996318
1106	70-71	0.1793545076182923
1106	72-73	0.18528052820293084
1106	74-75	0.22023821486279616
1106	76-77	0.15588510682568568
1106	78-79	0.1419919405475767
1106	80-81	0.16218926689891333
1106	82-83	0.18950624322952336
1106	84-85	0.1740261123353335
1106	86-87	0.2104324477051165
1106	88-89	0.1995931380206244
1106	90-91	0.20191338086688404
1106	92-93	0.19457156353072236
1106	94-95	0.20832477918563796
1106	96-97	0.14814108933899206
1106	98-99	0.2176927063571341
1106	100-101	0.2664043696702194
1107	1	-0.07630664494937633
1107	2	-0.12167261015164144
1107	3	-0.08247916794195476
1107	4	-0.04963157178058708
1107	5	-0.09930267182229358
1107	6	-0.0349054914511413
1107	7	-0.044287692835958126
1107	8	0.02075414774132156
1107	9	0.018478910317369923
1107	10-11	0.0698540750918113
1107	12-13	0.019058909626728848
1107	14-15	0.09343493369747335
1107	16-17	0.0416196232569348
1107	18-19	0.0487314759604871
1107	20-21	0.07347376232724656
1107	22-23	0.14836847879703186
1107	24-25	0.08156669209812151
1107	26-27	0.024398847110674637
1107	28-29	0.09246375053803035
1107	30-31	0.12322245725091108
1107	32-33	0.050930674417031696
1107	34-35	0.07761512039658669
1107	36-37	0.1318135205874711
1107	38-39	0.05146779257611911
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2216	74-75	-0.8974970828552031
2216	76-77	-0.8454215214227432
2216	78-79	-0.749735473937875
2216	80-81	-0.8229789808273367
2216	82-83	-0.9669531351250029
2216	84-85	-0.8549379388343183
2216	86-87	-0.8444513962040645
2216	88-89	-0.8984986111316431
2216	90-91	-0.9235237067885436
2216	92-93	-0.9989529593450825
2216	94-95	-0.9758058368135707
2216	96-97	-0.9285240237171735
2216	98-99	-0.9579317960534723
2216	100-101	-1.7058888419620217
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	23.0
10	133.0
11	106.0
12	30.0
13	17.0
14	45.0
15	71.0
16	733.0
17	5651.0
18	14178.0
19	14476.0
20	11798.0
21	13675.0
22	18715.0
23	26995.0
24	38390.0
25	52669.0
26	69410.0
27	89958.0
28	112941.0
29	140153.0
30	170954.0
31	207492.0
32	252288.0
33	308179.0
34	383987.0
35	491544.0
36	671504.0
37	1030590.0
38	2183537.0
39	6317488.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.55912614566394	12.442407849390268	6.2150406931718365	36.78342531177396
2	36.743429462252294	16.50139278325074	25.543609971269987	21.211567783226982
3	29.927104418196766	17.24636032919196	22.615386589246757	30.211148663364522
4	33.66317029978363	23.718364004422465	18.518862007026527	24.09960368876738
5	37.65711103784461	23.004794855076074	18.352075556962454	20.98601855011686
6	32.47590490044917	27.938837755768464	15.564925376976667	24.020331966805696
7	32.48240628027469	15.981709484789489	25.91536720532069	25.620517029615137
8	30.244535697532942	18.504291113525884	17.919603285332936	33.33156990360824
9	33.2709271257847	15.951287995223549	22.64851944836397	28.129265430627783
10-11	33.9343222428778	20.125040602676574	16.635695184545327	29.304941969900305
12-13	35.032881515588514	16.231249355037292	18.23478171135623	30.501087418017963
14-15	35.261066518286896	17.975846099520602	18.846471090437277	27.916616291755226
16-17	34.69568318578275	18.039705319340133	18.824574872224392	28.440036622652727
18-19	34.6295308754759	17.738150407849666	19.950407406199012	27.68191131047542
20-21	34.99587970545477	18.924307742210043	18.351525031640666	27.728287520694522
22-23	34.4428455283233	18.392877725521643	18.671848839033924	28.492427907121133
24-25	35.08831677215676	18.553700800920133	17.49857416925264	28.85940825767046
26-27	36.829610912254395	18.07829855891477	17.7018177204911	27.390272808339734
28-29	35.239575678916346	18.674183903037754	18.715589522655808	27.370650895390092
30-31	36.022501947503216	19.043671190660348	17.327231301771572	27.60659556006486
32-33	34.512202114100745	18.623094441754528	19.722144745213622	27.1425586989311
34-35	34.4213803184679	17.89731660683196	18.188987280979223	29.492315793720913
36-37	33.95798833938606	19.610482151173652	17.8084664613023	28.623063048137986
38-39	35.37647555116557	18.758476873809318	18.68914934019463	27.17589823483048
40-41	33.64907845254865	19.329544655876095	18.79524016460058	28.226136726974676
42-43	32.31421564580476	19.741717295485607	17.962809967259798	29.98125709144983
44-45	33.52355835795568	19.376783619453548	17.911389019368187	29.18826900322259
46-47	33.83395552269628	17.091939936424115	18.342764866429544	30.731339674450066
48-49	32.65629044313631	17.938072331318725	19.31277023216491	30.092866993380063
50-51	34.34563707299976	19.252012865327718	17.719526463634157	28.682823598038375
52-53	34.542961688040705	18.836031266894132	17.593121977022967	29.027885068042185
54-55	34.17364779385082	18.74254652575637	17.785523996761775	29.298281683631032
56-57	33.332730144706666	20.635291460480428	16.922925481205937	29.10905291360697
58-59	33.828370553914986	17.912429999562843	17.31640862832405	30.94279081819812
60-61	33.854240830763544	19.27226538512163	17.187539845671626	29.6859539384432
62-63	34.3240181739954	20.723241381618067	16.732339863297057	28.220400581089482
64-65	33.730819628728824	18.84426694203844	18.343111008613143	29.08180242061959
66-67	32.90056304792333	18.928706138166817	17.568908872642904	30.601821941266955
68-69	33.941625826286376	19.110490478563527	17.74744439501295	29.20043930013715
70-71	34.82006993433722	19.578616770357495	16.583022206724024	29.01829108858126
72-73	33.701006475428954	20.16426378460468	17.134549925236907	29.000179814729453
74-75	32.35700943083811	19.07646505309868	17.93209937529404	30.634426140769165
76-77	32.96352307421272	20.560573171781062	17.03898548091061	29.436918273095607
78-79	32.2527667309885	20.18465808739553	17.215235719809424	30.34733946180655
80-81	33.6134564590514	19.899263224028253	16.820146151402625	29.667134165517716
82-83	34.05498990614003	20.204446351157035	16.78282165647645	28.957742086226485
84-85	32.574901356666835	19.542897480034625	18.67363311550575	29.208568047792788
86-87	33.681185218061	20.91689724070266	16.797218040256716	28.604699500979613
88-89	33.63221392659044	19.248985145830257	17.032582523858185	30.08621840372112
90-91	33.13505701043195	18.619811001360173	18.735455360802085	29.509676627405796
92-93	33.810602757908384	20.499978952614793	16.926022064391695	28.76339622508513
94-95	33.51373464735596	21.442587223403724	16.499059800162687	28.544618329077625
96-97	32.30504194885545	21.295000881879123	17.934067279575956	28.46588988968948
98-99	32.51261356564905	19.141479628717768	17.98556362240341	30.36034318322977
100-101	34.53089301486988	20.275784207757464	16.447946290131576	28.745376487241074
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19875.0
1	19789.0
2	18034.0
3	14377.0
4	10723.0
5	7982.5
6	6106.0
7	4749.0
8	3718.5
9	2916.5
10	2400.0
11	1996.0
12	1621.5
13	1394.0
14	1238.0
15	1025.5
16	859.0
17	741.0
18	620.5
19	532.0
20	463.0
21	426.5
22	392.0
23	361.5
24	326.5
25	304.5
26	324.5
27	323.0
28	311.5
29	315.5
30	312.0
31	320.0
32	334.5
33	319.0
34	320.5
35	349.0
36	394.5
37	469.0
38	523.0
39	565.5
40	741.5
41	978.5
42	1221.0
43	1600.0
44	2459.5
45	3009.0
46	3396.5
47	4166.0
48	5736.0
49	11074.0
50	17675.0
51	23230.0
52	31426.0
53	53061.5
54	97751.0
55	124227.0
56	157125.0
57	262197.5
58	457379.0
59	840995.5
60	1184866.0
61	1185471.0
62	995772.0
63	970381.5
64	982311.5
65	879321.0
66	734358.0
67	610865.0
68	569553.5
69	497127.5
70	423583.5
71	357505.5
72	283080.5
73	214481.0
74	159246.5
75	115639.0
76	80411.0
77	55870.5
78	38415.0
79	24673.5
80	16334.5
81	11282.0
82	7440.0
83	4752.5
84	3001.5
85	1680.0
86	992.0
87	602.0
88	343.5
89	213.5
90	140.0
91	106.0
92	78.0
93	54.0
94	39.0
95	25.5
96	21.5
97	18.0
98	9.0
99	4.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03556456147850379
2	0.009685027541351622
3	0.009384102728128924
4	0.010286877167797023
5	0.008552599954750412
6	0.026006239121666993
7	0.0051315599728502465
8	0.006572831446706335
9	0.006256068485419282
10-11	0.006339218762757133
12-13	0.008235836993463358
14-15	0.012508177433822475
16-17	2.811271281422589E-4
18-19	3.721964795122864E-4
20-21	7.919074032176306E-6
22-23	6.929189778154268E-4
24-25	1.0294796241829198E-4
26-27	0.0010888726794242422
28-29	0.0017897107312718453
30-31	2.534103690296418E-4
32-33	0.0026687279488434154
34-35	0.0024747106350550955
36-37	8.077455512819832E-4
38-39	0.0
40-41	0.0015956934174835256
42-43	0.002348005450540275
44-45	0.001191820641842534
46-47	0.0012076587899068868
48-49	4.39508608785785E-4
50-51	0.0012314160120034156
52-53	0.003084479335532671
54-55	0.008528842732653882
56-57	0.003175548686902699
58-59	0.005345374971719006
60-61	0.007103409406862148
62-63	0.01153413132786479
64-65	0.007368698386940053
66-67	0.010861010035129804
68-69	0.00662430542791548
70-71	0.00934846689498413
72-73	0.006758929686462478
74-75	0.007273669498553938
76-77	0.007511241719519226
78-79	0.013042714930994376
80-81	0.007681501811211018
82-83	0.007946790791288924
84-85	0.010417541889327931
86-87	0.008714940972410025
88-89	0.0053730917308316235
90-91	0.005258265157365068
92-93	0.011621241142218729
94-95	0.009249478469581926
96-97	0.010231443649571789
98-99	0.007574594311776636
100-101	0.010940200775451568
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.2627739E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.579473932568856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.478352783410095	13.063511063885977
2	13.244551021942597	5.4513178500929165
3	6.41806541333114	3.962412296135099
4	3.6966753270997863	3.04302534124882
5	2.537590144838366	2.611113511862239
6	1.8629643248876016	2.3003295456798076
7	1.302565993791978	1.8764286040284857
8	0.9984207566859314	1.6437579148763037
9	0.8188783690435439	1.5166877444708502
>10	5.151052389717018	19.552293889054344
>50	0.32425134346617507	4.534775607399433
>100	0.1353646393597947	4.882437837947332
>500	0.011233136167846882	1.5989160623831304
>1k	0.012738608025393372	5.7755512291937405
>5k	0.0032425547701001307	4.694945108035358
>10k+	0.004053193462625163	23.492496393706098
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCC	133504	1.0572280595916657	No Hit
GGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCG	125074	0.9904702655004193	No Hit
GGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTC	101416	0.8031208120471922	No Hit
GTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGA	92578	0.7331320357508181	No Hit
GTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAG	89009	0.7048688605299809	No Hit
TATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTA	70225	0.5561169739095811	No Hit
GGCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTT	63319	0.5014278486433715	No Hit
GGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTC	59067	0.4677559458585579	No Hit
CAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGAC	58617	0.46419236254407853	No Hit
GGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCT	57788	0.45762745017140444	No Hit
GTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAG	56300	0.44584386801152603	No Hit
GGTGAGGCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGG	53969	0.42738450644252307	No Hit
GGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGAT	51723	0.4095982661662551	No Hit
CGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTA	49373	0.3909884421906408	No Hit
CATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGT	47864	0.3790385594760867	No Hit
GTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAG	44231	0.3502685635171902	No Hit
CGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGA	41366	0.32758041641500507	No Hit
GTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAG	40878	0.323715908287303	No Hit
GGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTG	40170	0.31810920387252223	No Hit
CTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGA	38770	0.3070225002274754	No Hit
GTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAA	37606	0.29780469805402215	No Hit
TGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCT	36512	0.2891412310628213	No Hit
GGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACC	36412	0.28834932365960364	No Hit
GTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCG	35898	0.2842789196070651	No Hit
GTGAGGCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGG	35720	0.28286932442933765	No Hit
GGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGG	34389	0.272329036892511	No Hit
GCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTG	34030	0.2694860893149597	No Hit
GATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGT	33792	0.26760134969530175	No Hit
CGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCG	29968	0.23731881059625956	No Hit
CGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATAT	29442	0.23315337765533484	No Hit
AGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTG	29229	0.2314666148864813	No Hit
CCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCT	29154	0.23087268433406805	No Hit
GTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGG	28766	0.22780008360958362	No Hit
GTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCG	28665	0.22700025713233382	No Hit
CTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGG	28180	0.2231595062267283	No Hit
AGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACG	27670	0.2191207784703184	No Hit
CTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAG	27398	0.21696679033356647	No Hit
CCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGG	26947	0.21339528794505494	No Hit
GTTGCGGGTCTGCGTAATTGCGAACAGCAGATGGCATCGACGGCCCTTTG	26859	0.21269840943022342	No Hit
GAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGT	26084	0.20656112705528676	No Hit
ATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTT	25198	0.19954482746277857	No Hit
GTTTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGA	25151	0.19917263098326626	No Hit
GCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTAGC	21905	0.173467316674822	No Hit
GTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTG	21802	0.17265165204950783	No Hit
CTTCTCCAAGATCAGGTTTCTCACCCACTTGGTGGGATAAGGCCCCCCGC	21750	0.17223986019983464	No Hit
GGCAGAAGTGTTGCGGGTCTGCGTAATTGCGAACAGCAGATGGCATCGAC	21429	0.16969783743550607	No Hit
GAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGG	21144	0.16744090133633582	No Hit
GGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACC	20691	0.16385356079975996	No Hit
GGCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCC	20096	0.15914171175061506	No Hit
GTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGT	19822	0.15697188546579874	No Hit
CAGCATGGTTCCCGGCACCCACGCGGAGTCATAGCCACGATAACGGCAGA	19762	0.15649674102386818	No Hit
CCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGG	19440	0.1539467991855074	No Hit
GTTCCCGGCACCCACGCGGAGTCATAGCCACGATAACGGCAGAAGTGTTG	19315	0.15295691493148536	No Hit
GTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCT	19058	0.15092171290521605	No Hit
GCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATG	18699	0.14807876532766476	No Hit
GGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAA	18523	0.1466850082980017	No Hit
CCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGG	18505	0.14654246496542256	No Hit
CGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCC	18269	0.14467356349382895	No Hit
GCGTAATTGCGAACAGCAGATGGCATCGACGGCCCTTTGGGTGGGGCTAC	18045	0.14289969091062144	No Hit
GAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTG	17993	0.14248789906094828	No Hit
CTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGC	17856	0.14140298591854011	No Hit
CAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGT	17773	0.1407457027738695	No Hit
GTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCC	17697	0.1401438531474241	No Hit
GCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCC	17577	0.13919356426356294	No Hit
TGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGT	17465	0.1383066279719592	No Hit
GCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATG	17416	0.13791859334438256	No Hit
GGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTAC	17167	0.13594674391037065	No Hit
GGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCAC	16822	0.13321466336926985	No Hit
CCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCATAGC	16739	0.13255738022459918	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16417	0.1300074383862384	No Hit
CGGTGAGGCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCG	16381	0.12972235172108007	No Hit
GCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCG	16058	0.12716449080868714	No Hit
AGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGC	15083	0.11944339362731522	No Hit
GGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCC	14998	0.11877027233458025	No Hit
ATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTC	14983	0.11865148622409759	No Hit
GAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTA	14933	0.11825553252248878	No Hit
CAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAG	14627	0.11583229586864283	No Hit
CAGGCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGG	14118	0.11180148718626509	No Hit
GTCATAGCCACGATAACGGCAGAAGTGTTGCGGGTCTGCGTAATTGCGAA	13711	0.10857842405516933	No Hit
GCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCG	13640	0.10801616979888481	No Hit
GTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAG	13564	0.10741432017243942	No Hit
CGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTAGCAGCGGGCCCG	13450	0.10651154573277131	No Hit
CGGCAGAAGTGTTGCGGGTCTGCGTAATTGCGAACAGCAGATGGCATCGA	13355	0.10575923369971457	No Hit
AAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGT	12967	0.10268663297523016	No Hit
GCCACGATAACGGCAGAAGTGTTGCGGGTCTGCGTAATTGCGAACAGCAG	12816	0.10149085279637154	No Hit
GTTGGCTGGGCGGCCGCGGCTCGCGTAGGGCTTGTGTGGATTCACGAGGT	12746	0.1009365176141192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.583814806435261E-5	0.0	1.583814806435261E-5	0.0	0.0
2	1.583814806435261E-5	0.0	8.710981435393937E-5	0.0	0.0
3	1.583814806435261E-5	0.0	8.710981435393937E-5	0.0	7.919074032176306E-6
4	7.919074032176306E-5	0.0	1.0294796241829198E-4	0.0	1.583814806435261E-5
5	1.1086703645046829E-4	0.0	1.6630055467570243E-4	0.0	1.583814806435261E-5
6	1.346242585469972E-4	0.0	1.9005777677223135E-4	0.0	1.583814806435261E-5
7	1.425433325791735E-4	0.0	2.0589592483658396E-4	0.0	1.583814806435261E-5
8	2.0589592483658396E-4	0.0	2.2173407290093658E-4	0.0	1.583814806435261E-5
9	2.2173407290093658E-4	0.0	2.2173407290093658E-4	0.0	1.583814806435261E-5
10-11	2.5341036902964183E-4	0.0	2.5736990604572995E-4	0.0	1.583814806435261E-5
12-13	3.3260110935140485E-4	0.0	2.7716759112617073E-4	0.0	1.583814806435261E-5
14-15	4.949421270110192E-4	0.0	2.85086665158347E-4	0.0	1.583814806435261E-5
16-17	6.493640706384571E-4	0.0	2.85086665158347E-4	0.0	2.375722209652892E-5
18-19	8.156646253141596E-4	0.0	3.0884388725487596E-4	0.0	3.959537016088153E-5
20-21	0.0010492773092633605	0.0	3.36560646367493E-4	0.0	4.3554907176969683E-5
22-23	0.0012749709191803853	0.0	3.721964795122864E-4	0.0	4.751444419305784E-5
24-25	0.0016036124915157022	0.0	4.117918496731679E-4	0.0	7.919074032176306E-5
26-27	0.0020233234152210464	0.0	4.5930629386622577E-4	0.0	7.919074032176306E-5
28-29	0.00242323665384595	0.0	4.5930629386622577E-4	0.0	8.315027733785121E-5
30-31	0.0029894504471465557	0.0	4.672253678984021E-4	0.0	1.1086703645046829E-4
32-33	0.003904103497862919	0.0	4.830635159627547E-4	0.0	1.346242585469972E-4
34-35	0.0050365310844641305	0.0	4.989016640271073E-4	0.0	1.425433325791735E-4
36-37	0.006283785244531899	0.0	5.226588861236362E-4	0.0	1.5838148064352612E-4
38-39	0.008010143383546334	0.0	5.384970341879888E-4	0.0	1.6630055467570243E-4
40-41	0.010429420500376197	0.0	5.780924043488704E-4	0.0	1.860982397561432E-4
42-43	0.014020720573968151	0.0	6.295663855580163E-4	0.0	2.1381499886876027E-4
44-45	0.0178812691646541	0.0	6.493640706384571E-4	0.0	2.1381499886876027E-4
46-47	0.022696066176217295	0.0	6.73121292734986E-4	0.0	2.1777453588484844E-4
48-49	0.028853146236234375	0.0	7.087571258797794E-4	0.0	2.2173407290093658E-4
50-51	0.03783733572573839	0.0	7.206357369280439E-4	0.0	2.2173407290093658E-4
52-53	0.04994164038391988	0.0	7.48352496040661E-4	0.0	2.2173407290093658E-4
54-55	0.06482554002739524	0.0	7.919074032176306E-4	0.0	2.2965314693311288E-4
56-57	0.08378380326042532	0.0	8.117050882980714E-4	0.0	2.375722209652892E-4
58-59	0.10707775952607193	0.0	8.235836993463359E-4	0.0	2.4945083201355367E-4
60-61	0.13722171482955103	0.0	8.51300458458953E-4	0.0	2.534103690296418E-4
62-63	0.18006786488064094	0.0	8.988149026520108E-4	0.0	2.5736990604572995E-4
64-65	0.23054008322471664	0.0	9.225721247485397E-4	0.0	2.613294430618181E-4
66-67	0.28891949698992037	0.0	9.582079578933331E-4	0.0	2.6528898007790623E-4
68-69	0.3593913368022573	0.0	9.81965179989862E-4	0.0	2.85086665158347E-4
70-71	0.45305814445483866	0.0	0.0010176010131346554	0.0	2.85086665158347E-4
72-73	0.5693022321731547	0.0	0.0010453177722472725	0.0	2.85086665158347E-4
74-75	0.7147360267740726	0.0	0.0010809536053920659	0.0	2.85086665158347E-4
76-77	0.8847387485598174	0.0	0.0011007512904725066	0.0	2.890462021744352E-4
78-79	1.0878827951702201	0.0	0.0011403466606333883	0.0	3.048843502387878E-4
80-81	1.3202996989405626	0.0	0.0011878611048264458	0.0	3.0884388725487596E-4
82-83	1.5983106714511601	0.0	0.0012274564749873275	0.0	3.0884388725487596E-4
84-85	1.9337547283801162	0.0	0.0012591327711160328	0.0	3.128034242709641E-4
86-87	2.3168636919087415	0.0	0.0012828899932125618	0.0	3.2468203531922857E-4
88-89	2.7891533076507207	0.0	0.0014214737887756471	0.0	3.36560646367493E-4
90	3.1881004192436984	0.0	0.0014254333257917352	0.0	3.4843925741575747E-4
>>END_MODULE