FastQCFastQC Report
Thu 25 Jan 2024
SRR25787977_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR25787977_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12627739
Sequences flagged as poor quality0
Sequence length101
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCC1335041.0572280595916657No Hit
GGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCG1250740.9904702655004193No Hit
GGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTC1014160.8031208120471922No Hit
GTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGA925780.7331320357508181No Hit
GTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAG890090.7048688605299809No Hit
TATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTA702250.5561169739095811No Hit
GGCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTT633190.5014278486433715No Hit
GGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTC590670.4677559458585579No Hit
CAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGAC586170.46419236254407853No Hit
GGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCT577880.45762745017140444No Hit
GTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAG563000.44584386801152603No Hit
GGTGAGGCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGG539690.42738450644252307No Hit
GGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGAT517230.4095982661662551No Hit
CGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTA493730.3909884421906408No Hit
CATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGT478640.3790385594760867No Hit
GTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAG442310.3502685635171902No Hit
CGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGA413660.32758041641500507No Hit
GTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAG408780.323715908287303No Hit
GGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTG401700.31810920387252223No Hit
CTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGA387700.3070225002274754No Hit
GTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAA376060.29780469805402215No Hit
TGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCT365120.2891412310628213No Hit
GGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACC364120.28834932365960364No Hit
GTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCG358980.2842789196070651No Hit
GTGAGGCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGG357200.28286932442933765No Hit
GGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGG343890.272329036892511No Hit
GCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTG340300.2694860893149597No Hit
GATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGT337920.26760134969530175No Hit
CGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCG299680.23731881059625956No Hit
CGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATAT294420.23315337765533484No Hit
AGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTG292290.2314666148864813No Hit
CCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCT291540.23087268433406805No Hit
GTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGG287660.22780008360958362No Hit
GTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCG286650.22700025713233382No Hit
CTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGG281800.2231595062267283No Hit
AGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACG276700.2191207784703184No Hit
CTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAG273980.21696679033356647No Hit
CCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGG269470.21339528794505494No Hit
GTTGCGGGTCTGCGTAATTGCGAACAGCAGATGGCATCGACGGCCCTTTG268590.21269840943022342No Hit
GAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGT260840.20656112705528676No Hit
ATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTT251980.19954482746277857No Hit
GTTTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGA251510.19917263098326626No Hit
GCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTAGC219050.173467316674822No Hit
GTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTG218020.17265165204950783No Hit
CTTCTCCAAGATCAGGTTTCTCACCCACTTGGTGGGATAAGGCCCCCCGC217500.17223986019983464No Hit
GGCAGAAGTGTTGCGGGTCTGCGTAATTGCGAACAGCAGATGGCATCGAC214290.16969783743550607No Hit
GAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGG211440.16744090133633582No Hit
GGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACC206910.16385356079975996No Hit
GGCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCC200960.15914171175061506No Hit
GTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGT198220.15697188546579874No Hit
CAGCATGGTTCCCGGCACCCACGCGGAGTCATAGCCACGATAACGGCAGA197620.15649674102386818No Hit
CCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGG194400.1539467991855074No Hit
GTTCCCGGCACCCACGCGGAGTCATAGCCACGATAACGGCAGAAGTGTTG193150.15295691493148536No Hit
GTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCT190580.15092171290521605No Hit
GCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAGCATG186990.14807876532766476No Hit
GGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAA185230.1466850082980017No Hit
CCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGG185050.14654246496542256No Hit
CGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCC182690.14467356349382895No Hit
GCGTAATTGCGAACAGCAGATGGCATCGACGGCCCTTTGGGTGGGGCTAC180450.14289969091062144No Hit
GAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTG179930.14248789906094828No Hit
CTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGC178560.14140298591854011No Hit
CAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGT177730.1407457027738695No Hit
GTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCC176970.1401438531474241No Hit
GCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCC175770.13919356426356294No Hit
TGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGT174650.1383066279719592No Hit
GCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATG174160.13791859334438256No Hit
GGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTAC171670.13594674391037065No Hit
GGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCAC168220.13321466336926985No Hit
CCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGGAGTCATAGC167390.13255738022459918No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA164170.1300074383862384No Hit
CGGTGAGGCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCG163810.12972235172108007No Hit
GCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCG160580.12716449080868714No Hit
AGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGC150830.11944339362731522No Hit
GGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCC149980.11877027233458025No Hit
ATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTC149830.11865148622409759No Hit
GAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTA149330.11825553252248878No Hit
CAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGGCGCCCAG146270.11583229586864283No Hit
CAGGCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGG141180.11180148718626509No Hit
GTCATAGCCACGATAACGGCAGAAGTGTTGCGGGTCTGCGTAATTGCGAA137110.10857842405516933No Hit
GCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCG136400.10801616979888481No Hit
GTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAG135640.10741432017243942No Hit
CGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTAGCAGCGGGCCCG134500.10651154573277131No Hit
CGGCAGAAGTGTTGCGGGTCTGCGTAATTGCGAACAGCAGATGGCATCGA133550.10575923369971457No Hit
AAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGT129670.10268663297523016No Hit
GCCACGATAACGGCAGAAGTGTTGCGGGTCTGCGTAATTGCGAACAGCAG128160.10149085279637154No Hit
GTTGGCTGGGCGGCCGCGGCTCGCGTAGGGCTTGTGTGGATTCACGAGGT127460.1009365176141192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)