FastQCFastQC Report
Thu 25 Jan 2024
SRR25787977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR25787977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12627739
Sequences flagged as poor quality0
Sequence length101
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCA2461801.949517645241163No Hit
GCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT1723461.3648207331494577No Hit
ATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGA1427661.1305745232776827No Hit
CTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGAT1178900.9335796376532648No Hit
CGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATT1017530.8057895399960358No Hit
CCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTA984120.7793319136545347No Hit
GTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGC770040.6098003767737042No Hit
CGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGGGCTCTCAC743970.5891553507718207No Hit
GGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCT736710.5834061030244607No Hit
CGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACC666780.5280280183174517No Hit
GGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGC660790.5232844929721782No Hit
CTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGGGCTCTC642040.5084362291618476No Hit
CGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACA611680.4843939204001603No Hit
CCGGGCTCTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACC555080.43957196137804244No Hit
CTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTG504900.39983404788458177No Hit
ATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGC468580.3710719709997174No Hit
CCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACT459440.3638339373343082No Hit
GAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTC450650.35687307126002527No Hit
CCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTGTA444990.35239087535781344No Hit
CTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCG442240.350213129998965No Hit
CCACGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTT404430.32027111108330636No Hit
CACGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTT404300.3201681631208881No Hit
GAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCAC363250.2876603642188043No Hit
CCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTAC352920.27947996074356624No Hit
GGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCG326190.25831227585555894No Hit
CGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTC303200.24010632465558562No Hit
CCGCTATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGG299810.23742175855867784No Hit
CGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCG288060.2281168465708707No Hit
GTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCT287260.22748332064829657No Hit
ACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTAC282040.22334956400350053No Hit
GTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAG276060.21861395773225914No Hit
GGCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTGA270130.21391794683117857No Hit
GCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCG268290.21246083720925812No Hit
CTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAG253230.2005347117168006No Hit
GGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGATA252970.20032881579196402No Hit
GTCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT240040.19008945306836006No Hit
GTTCTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGC229250.1815447721876418No Hit
CCGCAACACTTCTGCCGTTATCGTGGCTATGACTCCGCGTGGGTGCCGGG222350.17608061110544018No Hit
GTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACACTTTCTG220960.17497985981496766No Hit
CACGGTTCTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCT220840.17488483092658155No Hit
CCGCTAACCACGACACTTTCTGACTGCCTCTCAGCCGGGTAGCGCTGAGA215600.17073523613372119No Hit
GCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAG200030.15840523786562266No Hit
GCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTG195090.15449321529372756No Hit
CGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGAT190350.150739574202476No Hit
GCCACTTCCGCTAACCACGACACTTTCTGACTGCCTCTCAGCCGGGTAGC190280.15068414068425076No Hit
CTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGC187300.14832425662266224No Hit
CGGGCTCTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCA182280.14434888145850971No Hit
CCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTAT177100.1402468011098424No Hit
CGGCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTG173160.13712668594116492No Hit
CCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCC172790.1368336802019744No Hit
CATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGG171240.13560622372698708No Hit
CTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAA168340.13330969225765593No Hit
CTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACAC167630.13274743800137143No Hit
GCCATCTGCTGTTCGCAATTACGCAGACCCGCAACACTTCTGCCGTTATC164770.13048258282816902No Hit
CACTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGC156940.12428194786097495No Hit
GCCTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCG155920.12347420230969298No Hit
GCCGGGTTTTCGCGTACCGGGCTCTCACCGTCTACGGCAGACCATCCCAG155380.12304657231195545No Hit
CACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACACTT154560.12239720824131699No Hit
CCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT153180.12130437602487665No Hit
GTCGATGCCATCTGCTGTTCGCAATTACGCAGACCCGCAACACTTCTGCC150360.11907119714780294No Hit
CTCTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGAC149920.11872275789038718No Hit
CTCGGCGATCTTGCGACGCTTCTAGCTGTAGCCCCACCCAAAGGGCCGTC149180.11813674641200614No Hit
CTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGG144230.11421680476607887No Hit
GCCTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCC142990.11323483958608901No Hit
CCCAGTCGCTGTGGTTGTCCCGAGGAGTCCCGCGACCGGACTCATCGGTG131360.10402495648666797No Hit
CGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACACTTTCTGACTGC130920.10367651722925221No Hit
GGCAGGTGCCGGGTTTTCGCGTACCGGGCTCTCACCGTCTACGGCAGACC127740.10115825168702014No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)