FastQCFastQC Report
Thu 25 Jan 2024
SRR25787976_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR25787976_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14982631
Sequences flagged as poor quality0
Sequence length101
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCC2452741.6370556012492063No Hit
GGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCG2275361.5186651797004145No Hit
GGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTC1889451.2610935956441829No Hit
GTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGA1886401.2590579051169317No Hit
GTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAG1857921.2400492276690256No Hit
CAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGAC1486710.9922890045146275No Hit
TATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTA1437620.9595243986186405No Hit
GTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAG1366720.9122029368540144No Hit
CGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTA1219400.8138757471901964No Hit
GGCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTT1213670.8100513187570327No Hit
CATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGT1160640.7746570011635473No Hit
GGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTC1135330.7577641069849481No Hit
GGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCT1113040.7428868801480861No Hit
CGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGA1070670.714607467807223No Hit
GGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGAT1035440.6910935736186788No Hit
CTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGA980230.6542442378778467No Hit
CTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAG954630.6371577862392793No Hit
CGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATAT888740.5931801964554824No Hit
GTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAG877750.5858450361622067No Hit
CCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCT844920.5639329968147784No Hit
TGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCT822750.5491358627199722No Hit
GTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAG815260.544136740736657No Hit
GGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTG807040.5386503879058357No Hit
GTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCG770130.5140151953285107No Hit
CGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCG755830.5044708102335298No Hit
CTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGG722120.4819714241110256No Hit
GGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACC698600.4662732466680919No Hit
CCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGG662060.44188500671210557No Hit
GTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCG597800.39899534334123293No Hit
GTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAA595690.39758704596008543No Hit
GCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTG593770.39630556208719286No Hit
CTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGC581930.38840307820435543No Hit
AGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACG571530.38146170722618744No Hit
GGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGG561480.37475394007901547No Hit
GATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGT543880.36300700457750046No Hit
AGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTG527790.352267902746854No Hit
GAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGG518740.34622757511681357No Hit
CCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGG506360.3379646738947252No Hit
GTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCT499650.33348615473477256No Hit
CCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGG497950.33235150755564896No Hit
ATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTT497580.33210455493431024No Hit
GGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACC496780.331570603320605No Hit
GAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGT475520.3173808391863886No Hit
GTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGT472770.31554538051427683No Hit
GCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTAGC460770.3075361063086984No Hit
GGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCAC456760.3048596738450009No Hit
CTTCTCCAAGATCAGGTTTCTCACCCACTTGGTGGGATAAGGCCCCCCGC437560.29204483511607543No Hit
GCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCG431860.28824042986842563No Hit
CGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCC418120.2790698109030383No Hit
GTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCC390600.26070187539157846No Hit
CAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGT371120.2477001535978561No Hit
ATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTC363510.2426209388724851No Hit
GTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTG361770.2414595941126762No Hit
GAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTG346530.23128781587159158No Hit
GAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTA344380.22985282090975875No Hit
GCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATG332970.2222373360192879No Hit
GGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTAC329540.21994801847552678No Hit
CGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTAGCAGCGGGCCCG329350.21982120496727175No Hit
GTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAG327700.21871992976400476No Hit
TGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGT316340.21113781684939048No Hit
CAGGCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGG314070.2096227291455019No Hit
AAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGT305460.20387607490299936No Hit
GTTTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGA288430.19250957992624926No Hit
GGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCC287810.19209576742562773No Hit
CGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCC285450.19052061016519728No Hit
GGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAA284490.189879868228751No Hit
CAGTTGTCCCGCCAGGGGCACCGCTGGATAGCCACGTTCGGTCAGGATAA273880.18279833495198541No Hit
AACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCG272270.18172375732940363No Hit
CGTGTGTGGGCGCCCGTGACGAATCAGCGGTACTAACCACCCAAAACCGG267360.1784466293002878No Hit
AGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGC267270.17838655974374595No Hit
GTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAG266400.1778058873638415No Hit
GCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCG264140.17629747405512425No Hit
GGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGG250430.16714687827525085No Hit
GCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATG247710.16533144278865308No Hit
GTTGTCCCGCCAGGGGCACCGCTGGATAGCCACGTTCGGTCAGGATAACC246390.16445042262603946No Hit
GGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCC239580.15990515951437367No Hit
CCTTCTCCAAGATCAGGTTTCTCACCCACTTGGTGGGATAAGGCCCCCCG237040.15820986314085955No Hit
ACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGC231630.15459901535317797No Hit
GTTGGCTGGGCGGCCGCGGCTCGCGTAGGGCTTGTGTGGATTCACGAGGT227670.15195595486533708No Hit
GAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTC224360.14974673006363168No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA221370.14775108590740837No Hit
GTCAGTGGTAACACTGGGGCAAGCCGGTAGGGAGAGCGATAGGCAAATCC221180.14762427239915338No Hit
ATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAG217880.14542172199261932No Hit
GCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGC216590.14456072501551961No Hit
TGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGC212380.14175080464839587No Hit
TGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGA198100.1322197683437575No Hit
CTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGT192880.1287357340643309No Hit
GGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGA187110.12488460805048192No Hit
AGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACG183710.12261531369223469No Hit
GTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGC182090.12153406167448161No Hit
CGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGG176920.11808339937091154No Hit
TAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGC174400.11640145178774008No Hit
GGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCA172580.11518671186656068No Hit
GTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAAC171710.11460603948665626No Hit
GGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGC168880.112717185653174No Hit
GAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAA155360.10369340338155562No Hit
AGGCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGT153790.1026455233396591No Hit
GCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTC152610.10185794470944388No Hit
CGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTAGCAGC152360.101691084830161No Hit
GGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGA152060.10149085297502154No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)