FastQCFastQC Report
Thu 25 Jan 2024
SRR25787976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR25787976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14982631
Sequences flagged as poor quality0
Sequence length101
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACA5588613.7300591598364803No Hit
ATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGA5034103.3599572731918714No Hit
GGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCA4733983.159645325310354No Hit
GTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGC3601562.403823467320259No Hit
CCGCTATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGG3226232.1533133933552793No Hit
CGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATT2960171.9757344354272626No Hit
GCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT2674831.7852872436089497No Hit
CCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTA2198541.4673924759943697No Hit
CGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACC1175170.7843548973474685No Hit
GAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTC987650.6591966390949627No Hit
ATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCA960750.6412425160841243No Hit
GGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGC938210.6261984293679795No Hit
ATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGC923130.6161334414496359No Hit
CTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGGGCTCTC856790.5718555038831298No Hit
CCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACT854280.5701802306951296No Hit
GGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCT842830.5625380482239735No Hit
CGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGGGCTCTCAC776120.5180131580361286No Hit
CATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGG755580.5043039503542469No Hit
GAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCAC676160.45129590390366014No Hit
CCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTAC664620.4435936518759623No Hit
CCACGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTT650380.43408931315200916No Hit
GGCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTGA585460.3907591396998297No Hit
CACGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTT564260.37660942193664115No Hit
CGGCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTG551560.36813294006907066No Hit
ATCCGCTATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGC542590.3621460076004008No Hit
GGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCG542380.36200584530180313No Hit
GGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAG460380.3072758048970171No Hit
CGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTC435420.29061651454941395No Hit
CGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGAT426570.2847096748227998No Hit
CTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCG423350.2825605195776363No Hit
TCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGAT419940.2802845508242177No Hit
CGCTATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGA407840.27220853266692613No Hit
CGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCG403190.2691049389122645No Hit
ACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTAC397800.2655074399149255No Hit
TATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTC388640.2593936939380006No Hit
GGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGATA385240.25712439957975336No Hit
CCGGGCTCTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACC378300.2524923693308605No Hit
CTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAA367820.24549760319132202No Hit
CCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGA355880.23752837535677146No Hit
TCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCG332560.22196368581726403No Hit
CTATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATT331790.22144975738907272No Hit
GCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCG323880.21617031080856225No Hit
GTCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT307460.2052109539372624No Hit
GTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAG282440.18851161721863136No Hit
CTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGC279990.1868763904016591No Hit
CCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTAT279980.18686971600648777No Hit
CACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACACTT265650.17730530772599287No Hit
GTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAAT259860.17344083292180124No Hit
GTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACACTTTCTG258430.17248639441230315No Hit
CTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACAC254580.16991675227134673No Hit
CTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAG249850.16675976335531456No Hit
GTCGGTTTGGGGTACGGGCCGTGTGTGTGCTCGCTAGAGGCTTTTCTTGG246670.1646373056908363No Hit
CTTCGCTTGACTACTACCAGCGAAGTTCCCACGCAGCCCCAGAACTCCAC239430.15980504358680395No Hit
CCCGTGTTCTGCGGGGGGCCTTATCCCACCAAGTGGGTGAGAAACCTGAT235620.1572620990265328No Hit
GGGGTGTGGACGCACGGCTAGCGCCGTGAACGGATGTGGTTGCGAGTTTG224860.15008044982219745No Hit
GCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTG224800.15004040345116954No Hit
CCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT222120.14825166554525704No Hit
GCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTGAC217910.14544174517813327No Hit
CCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATG212160.14160396795462693No Hit
GTTGCGAGTTTGTTTTTGCCTCCCTTTTCCAAAAGGGAGGGTGTTGTAAG201490.13448238830683343No Hit
CCGCTAACCACGACACTTTCTGACTGCCTCTCAGCCGGGTAGCGCTGAGA190540.1271739255942431No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA188050.12551200119658557No Hit
GTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGG179550.1198387653009675No Hit
CTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGG177870.11871746691218651No Hit
AGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCC171150.1142322733570626No Hit
GCCGTGTGTGTGCTCGCTAGAGGCTTTTCTTGGCAGCAGAGGATCACCGA168390.11239014028977955No Hit
AGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACG162140.10821864330770743No Hit
CTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGAT159820.10667018362796228No Hit
CCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCC156240.10428075015663137No Hit
CTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTG154610.10319282374370697No Hit
CTCTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGAC154020.10279903442859935No Hit
GCCACTTCCGCTAACCACGACACTTTCTGACTGCCTCTCAGCCGGGTAGC152980.10210489733078255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)