##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25787976_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14982631
Sequences flagged as poor quality	0
Sequence length	101
%GC	61
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.88415212254777	35.0	35.0	35.0	31.0	35.0
2	33.934133664507925	35.0	35.0	35.0	31.0	35.0
3	33.947261866090145	35.0	35.0	35.0	31.0	35.0
4	34.04432639367545	35.0	35.0	35.0	31.0	35.0
5	33.98658646802421	35.0	35.0	35.0	31.0	35.0
6	38.57883218241175	40.0	39.0	40.0	36.0	40.0
7	38.62245135717485	40.0	39.0	40.0	36.0	40.0
8	38.55106376176521	40.0	39.0	40.0	36.0	40.0
9	38.6403610954578	40.0	39.0	40.0	36.0	40.0
10-11	38.600581433260956	40.0	39.0	40.0	36.0	40.0
12-13	38.60683040248405	40.0	39.0	40.0	36.0	40.0
14-15	38.599006476232375	40.0	39.0	40.0	36.0	40.0
16-17	38.59578157534548	40.0	39.0	40.0	36.0	40.0
18-19	38.59935724907061	40.0	39.0	40.0	36.0	40.0
20-21	38.58741502076638	40.0	39.0	40.0	36.0	40.0
22-23	38.58071272662325	40.0	39.0	40.0	36.0	40.0
24-25	38.61613547714016	40.0	39.0	40.0	36.0	40.0
26-27	38.560937962097576	40.0	39.0	40.0	36.0	40.0
28-29	38.54386792947113	40.0	39.0	40.0	36.0	40.0
30-31	38.5297009917684	40.0	39.0	40.0	36.0	40.0
32-33	38.530934153020254	40.0	39.0	40.0	36.0	40.0
34-35	38.48826975048641	40.0	39.0	40.0	35.0	40.0
36-37	38.47861196741747	40.0	39.0	40.0	35.5	40.0
38-39	38.419993257526	40.0	39.0	40.0	35.0	40.0
40-41	38.40218290098715	40.0	39.0	40.0	35.0	40.0
42-43	38.371750362136	40.0	39.0	40.0	35.0	40.0
44-45	38.354446592190655	40.0	39.0	40.0	35.0	40.0
46-47	38.33680406331838	40.0	39.0	40.0	35.0	40.0
48-49	38.33208880336171	40.0	39.0	40.0	35.0	40.0
50-51	38.3247077899736	40.0	39.0	40.0	35.0	40.0
52-53	38.320835539499036	40.0	39.0	40.0	35.0	40.0
54-55	38.23823722949594	40.0	39.0	40.0	35.0	40.0
56-57	38.228500555076074	40.0	39.0	40.0	35.0	40.0
58-59	38.22955330742644	40.0	39.0	40.0	35.0	40.0
60-61	38.2274838778316	40.0	39.0	40.0	35.0	40.0
62-63	38.214766218296376	40.0	39.0	40.0	35.0	40.0
64-65	38.219897459932106	40.0	39.0	40.0	35.0	40.0
66-67	38.20620497161013	40.0	39.0	40.0	35.0	40.0
68-69	38.192394880445235	40.0	39.0	40.0	35.0	40.0
70-71	38.12130733247051	40.0	39.0	40.0	34.5	40.0
72-73	38.11887575019367	40.0	39.0	40.0	34.0	40.0
74-75	38.07849192174592	40.0	39.0	40.0	34.0	40.0
76-77	38.03929563505902	40.0	39.0	40.0	34.0	40.0
78-79	38.025859276651744	40.0	39.0	40.0	34.0	40.0
80-81	37.99609184127941	40.0	39.0	40.0	34.0	40.0
82-83	37.97827791393915	40.0	39.0	40.0	34.0	40.0
84-85	37.9779564750677	40.0	39.0	40.0	34.0	40.0
86-87	37.96615624452074	40.0	39.0	40.0	34.0	40.0
88-89	37.9015106559055	40.0	39.0	40.0	34.0	40.0
90-91	37.914970608299704	40.0	39.0	40.0	34.0	40.0
92-93	37.89317043181535	40.0	39.0	40.0	34.0	40.0
94-95	37.8472587357988	40.0	39.0	40.0	34.0	40.0
96-97	37.812849692420514	40.0	39.0	40.0	34.0	40.0
98-99	37.70056781082041	39.5	38.0	40.0	34.0	40.0
100-101	36.674507868477846	39.0	36.5	40.0	30.5	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.29387755526508386
1101	2	0.31531655225516886
1101	3	0.2797559621440442
1101	4	0.31807006253425385
1101	5	0.3182885305337706
1101	6	0.3737683906785705
1101	7	0.3657511827432671
1101	8	0.35208697910375975
1101	9	0.25586871642720155
1101	10-11	0.3431804405252592
1101	12-13	0.3325378677744766
1101	14-15	0.35456809189341243
1101	16-17	0.3719913215142796
1101	18-19	0.34074394627650406
1101	20-21	0.36400161661482144
1101	22-23	0.385151944596295
1101	24-25	0.33904365921308965
1101	26-27	0.39824612094510314
1101	28-29	0.39245225195289635
1101	30-31	0.40796136642840963
1101	32-33	0.4131952879059142
1101	34-35	0.4039843931407603
1101	36-37	0.395931987633638
1101	38-39	0.44274238647759034
1101	40-41	0.4215805830844701
1101	42-43	0.45426425915629665
1101	44-45	0.4310157744167782
1101	46-47	0.44388837259035085
1101	48-49	0.4134766700798167
1101	50-51	0.4109806772185749
1101	52-53	0.40344252739306796
1101	54-55	0.4330361498712634
1101	56-57	0.42235918733464217
1101	58-59	0.4303179618554722
1101	60-61	0.4073667034340147
1101	62-63	0.41149001334228075
1101	64-65	0.40232289743839544
1101	66-67	0.3500169710633969
1101	68-69	0.38987711183374074
1101	70-71	0.4261774733285435
1101	72-73	0.40489478047029337
1101	74-75	0.4118715224801974
1101	76-77	0.3305730898335355
1101	78-79	0.35817112780125626
1101	80-81	0.34824499126744257
1101	82-83	0.3739642874900113
1101	84-85	0.38704048910712885
1101	86-87	0.379962203147457
1101	88-89	0.40189739986082884
1101	90-91	0.33691380520550496
1101	92-93	0.37439806944163223
1101	94-95	0.3712470998156334
1101	96-97	0.3830778076987258
1101	98-99	0.40298651991009393
1101	100-101	0.32050713128484887
1102	1	0.29646589112589794
1102	2	0.3249327417928427
1102	3	0.29163705199133005
1102	4	0.2673759999720531
1102	5	0.26871885831607045
1102	6	0.22065237852471142
1102	7	0.15027667238928188
1102	8	0.18332007691982
1102	9	0.18177179326312398
1102	10-11	0.2515725820363599
1102	12-13	0.2594078621761895
1102	14-15	0.3214044098399569
1102	16-17	0.2806436181439622
1102	18-19	0.2888911867961639
1102	20-21	0.32837811099386727
1102	22-23	0.3348289676474039
1102	24-25	0.35010100123052723
1102	26-27	0.3086484518083523
1102	28-29	0.3069672459026336
1102	30-31	0.3291782698432755
1102	32-33	0.2900138915439996
1102	34-35	0.27039790315706114
1102	36-37	0.2994419180008663
1102	38-39	0.33283296561697284
1102	40-41	0.3325698836751414
1102	42-43	0.3259713816982597
1102	44-45	0.34252544431127063
1102	46-47	0.34284077222223175
1102	48-49	0.33195203102685866
1102	50-51	0.31607207454237596
1102	52-53	0.28694038230999297
1102	54-55	0.3764533103954122
1102	56-57	0.2908471255755387
1102	58-59	0.33024885500024936
1102	60-61	0.32044704557787185
1102	62-63	0.3539184217039377
1102	64-65	0.33297837588771273
1102	66-67	0.3081007774657323
1102	68-69	0.33733310325789034
1102	70-71	0.30906667771748886
1102	72-73	0.2643574028715534
1102	74-75	0.3016595946051126
1102	76-77	0.3026851242280628
1102	78-79	0.2848203990821787
1102	80-81	0.31461921862424447
1102	82-83	0.27882049816730614
1102	84-85	0.2917058811008246
1102	86-87	0.29763330937058186
1102	88-89	0.26571279793003555
1102	90-91	0.2637134244909092
1102	92-93	0.23384457110783075
1102	94-95	0.263936134157305
1102	96-97	0.21735027201548007
1102	98-99	0.23054174418228257
1102	100-101	0.24154416340503104
1103	1	0.20973945039520459
1103	2	0.2430974945899962
1103	3	0.24880679230462022
1103	4	0.20120958344180195
1103	5	0.2620755128467138
1103	6	0.27378012058833434
1103	7	0.27088947766076643
1103	8	0.25154897754941175
1103	9	0.2593368067613113
1103	10-11	0.281423634934832
1103	12-13	0.2963504276923885
1103	14-15	0.2879194087657737
1103	16-17	0.2876344397828703
1103	18-19	0.26621454494304686
1103	20-21	0.2608925884645217
1103	22-23	0.3151120328543371
1103	24-25	0.3116165684301677
1103	26-27	0.2973249833472238
1103	28-29	0.2892261097880464
1103	30-31	0.3000078735717864
1103	32-33	0.2944306091878559
1103	34-35	0.2727963932369235
1103	36-37	0.2336867119761834
1103	38-39	0.31602058183173654
1103	40-41	0.3303620623343093
1103	42-43	0.3219281663001823
1103	44-45	0.2767763026150263
1103	46-47	0.3042678391993263
1103	48-49	0.3141386315436989
1103	50-51	0.30442407028974117
1103	52-53	0.2812749130141441
1103	54-55	0.31922926329285417
1103	56-57	0.30282125236663404
1103	58-59	0.28610532665884136
1103	60-61	0.3326712466869637
1103	62-63	0.35340257262438257
1103	64-65	0.34651690348281505
1103	66-67	0.3104934484027453
1103	68-69	0.28186302932259366
1103	70-71	0.2610012237589032
1103	72-73	0.2652048028344183
1103	74-75	0.2519465534021279
1103	76-77	0.20888431031639243
1103	78-79	0.2967143298405972
1103	80-81	0.33053634628693374
1103	82-83	0.3138387047288518
1103	84-85	0.3163741387800343
1103	86-87	0.27295974968669867
1103	88-89	0.30249467480268777
1103	90-91	0.3077536665264873
1103	92-93	0.36869762943175033
1103	94-95	0.295913053237598
1103	96-97	0.31688235918242214
1103	98-99	0.30102758143143404
1103	100-101	0.3760607285892732
1104	1	0.22336516998509381
1104	2	0.2169164148057945
1104	3	0.20225072946936962
1104	4	0.1748366862101136
1104	5	0.18034862461879442
1104	6	0.17425962053888355
1104	7	0.18924477533862216
1104	8	0.2333090160243927
1104	9	0.2462717862092063
1104	10-11	0.2638397036906639
1104	12-13	0.2412689096236207
1104	14-15	0.26792431412734175
1104	16-17	0.261773598535477
1104	18-19	0.23336244522661076
1104	20-21	0.24491487990388094
1104	22-23	0.2617265775444153
1104	24-25	0.2405799063986933
1104	26-27	0.3006508414279949
1104	28-29	0.27436684047975746
1104	30-31	0.25882800882322954
1104	32-33	0.259347132337318
1104	34-35	0.2356671186842192
1104	36-37	0.22396806945390324
1104	38-39	0.2766617707370074
1104	40-41	0.2546187573798804
1104	42-43	0.2884583255157267
1104	44-45	0.31269358604737363
1104	46-47	0.28561934581251336
1104	48-49	0.28450615999271633
1104	50-51	0.26472121947624316
1104	52-53	0.289107728141218
1104	54-55	0.2671872994093363
1104	56-57	0.241818931131057
1104	58-59	0.26650553692220313
1104	60-61	0.23606973183805025
1104	62-63	0.2619122901833606
1104	64-65	0.2392985698208676
1104	66-67	0.2070887766584235
1104	68-69	0.31829761095772113
1104	70-71	0.23264054420610591
1104	72-73	0.18460518543597004
1104	74-75	0.23055862436908825
1104	76-77	0.21962168791129955
1104	78-79	0.23380906084659614
1104	80-81	0.22433730885374814
1104	82-83	0.17234692410300312
1104	84-85	0.16426483330472763
1104	86-87	0.204881814952806
1104	88-89	0.19763851254445086
1104	90-91	0.21145560858538914
1104	92-93	0.2785298470969195
1104	94-95	0.2734944497701548
1104	96-97	0.2460170599475333
1104	98-99	0.25443442923717186
1104	100-101	0.2571672703523049
1105	1	0.18702048923384496
1105	2	0.17135402282054457
1105	3	0.15462375628632685
1105	4	0.11475036658090687
1105	5	0.14319482819634288
1105	6	0.22500540834953853
1105	7	0.21002784402476493
1105	8	0.20130018131687422
1105	9	0.23040698860810238
1105	10-11	0.20735817283654256
1105	12-13	0.19582169007932748
1105	14-15	0.2178948915293759
1105	16-17	0.19477448665919184
1105	18-19	0.2161338236160546
1105	20-21	0.19046905607604714
1105	22-23	0.21106989844822266
1105	24-25	0.20000271180626328
1105	26-27	0.2469100582877246
1105	28-29	0.24118677951051382
1105	30-31	0.23171510981195098
1105	32-33	0.22152505134527445
1105	34-35	0.21170064131832333
1105	36-37	0.19952487095311255
1105	38-39	0.24589723674814934
1105	40-41	0.21549633899396525
1105	42-43	0.20915345161522936
1105	44-45	0.22125169131981437
1105	46-47	0.22327415096439296
1105	48-49	0.2158117580470531
1105	50-51	0.22367283996959486
1105	52-53	0.20476094378005882
1105	54-55	0.22234734573809334
1105	56-57	0.19047743577533538
1105	58-59	0.21165259689748694
1105	60-61	0.2279169570089934
1105	62-63	0.23913974008561212
1105	64-65	0.2366680375425929
1105	66-67	0.19913481411472134
1105	68-69	0.16777830883864908
1105	70-71	0.18988044781467295
1105	72-73	0.16644447362538983
1105	74-75	0.18458874575857465
1105	76-77	0.14050941079638335
1105	78-79	0.21034187573155805
1105	80-81	0.19297412602216468
1105	82-83	0.24850460278818787
1105	84-85	0.23809834900750104
1105	86-87	0.1671274636651887
1105	88-89	0.1842966632708567
1105	90-91	0.17528588837967618
1105	92-93	0.23272026872697893
1105	94-95	0.20457193898303672
1105	96-97	0.23305444275337095
1105	98-99	0.20774174188425576
1105	100-101	0.27480672014086593
1106	1	0.19483471216272363
1106	2	0.14519119301075278
1106	3	0.14456775189695747
1106	4	0.1381937034159577
1106	5	0.12293111002622936
1106	6	0.20907764192914158
1106	7	0.13825408544936835
1106	8	0.19019990345920235
1106	9	0.16991445724719512
1106	10-11	0.16763627992538943
1106	12-13	0.14128220792679258
1106	14-15	0.14974758057613258
1106	16-17	0.14197675447802993
1106	18-19	0.1263487381879429
1106	20-21	0.13932123739683533
1106	22-23	0.16893650674263228
1106	24-25	0.13880247766263665
1106	26-27	0.19256672909943973
1106	28-29	0.1689329602396228
1106	30-31	0.16078370248179397
1106	32-33	0.1569663203135221
1106	34-35	0.1802510956339205
1106	36-37	0.17478022902031398
1106	38-39	0.22022822502658812
1106	40-41	0.17678163552262305
1106	42-43	0.13877689153289907
1106	44-45	0.1306423148950202
1106	46-47	0.15147586822139658
1106	48-49	0.11789930015807215
1106	50-51	0.15319028868314177
1106	52-53	0.13168154513338237
1106	54-55	0.1844549548123453
1106	56-57	0.18428680175360768
1106	58-59	0.17639228095664805
1106	60-61	0.18861037181438434
1106	62-63	0.17278080851637156
1106	64-65	0.15620756553169457
1106	66-67	0.11828409905010062
1106	68-69	0.136739602838162
1106	70-71	0.14259472991997768
1106	72-73	0.1271983789304656
1106	74-75	0.19233099656437247
1106	76-77	0.13497545001814615
1106	78-79	0.10368433184112291
1106	80-81	0.1286953311371022
1106	82-83	0.11639840129562629
1106	84-85	0.1263817019649025
1106	86-87	0.16282572186749888
1106	88-89	0.13227884366655474
1106	90-91	0.166905487073997
1106	92-93	0.2263696169204934
1106	94-95	0.19392551101453392
1106	96-97	0.16262655764884926
1106	98-99	0.2104028265047262
1106	100-101	0.2641181619924353
1107	1	0.1180112606342334
1107	2	0.09904531968107477
1107	3	0.09875703737334618
1107	4	0.07559266693449729
1107	5	0.09824063641396208
1107	6	0.17524647295499562
1107	7	0.003869308153042539
1107	8	0.08054649707617045
1107	9	0.0204266571863414
1107	10-11	0.08777613398006423
1107	12-13	0.050399522405854214
1107	14-15	0.07746298029319121
1107	16-17	0.06624352141820822
1107	18-19	0.09696435856890417
1107	20-21	0.13079651399736036
1107	22-23	0.16170288299597502
1107	24-25	0.14376991636864744
1107	26-27	0.15718525507557501
1107	28-29	0.14421612070341894
1107	30-31	0.10439228011694723
1107	32-33	0.08257172200813301
1107	34-35	0.09381916012985414
1107	36-37	0.11110240451756681
1107	38-39	0.14042046901437288
1107	40-41	0.1168425692073285
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2216	56-57	-0.6826811596779478
2216	58-59	-0.7645314838771853
2216	60-61	-0.6812890126087083
2216	62-63	-0.6617083018347145
2216	64-65	-0.6586472533820569
2216	66-67	-0.6788690426204269
2216	68-69	-0.6757088740295814
2216	70-71	-0.7036914979873856
2216	72-73	-0.6704939995294765
2216	74-75	-0.7396375358958167
2216	76-77	-0.6502043558601258
2216	78-79	-0.6696615231199559
2216	80-81	-0.6464643637391347
2216	82-83	-0.6579629272670786
2216	84-85	-0.6305217564740744
2216	86-87	-0.692568775223819
2216	88-89	-0.5962709828891448
2216	90-91	-0.6018745762356517
2216	92-93	-0.6264750088250537
2216	94-95	-0.6917267373008684
2216	96-97	-0.655513302756404
2216	98-99	-0.6487824955206136
2216	100-101	-0.8360446558555736
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	25.0
17	172.0
18	146.0
19	148.0
20	208.0
21	578.0
22	1759.0
23	4773.0
24	10959.0
25	21711.0
26	37760.0
27	60763.0
28	89526.0
29	125332.0
30	167702.0
31	216226.0
32	272944.0
33	342614.0
34	430964.0
35	550758.0
36	737948.0
37	1094931.0
38	2191330.0
39	8623354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.090834352178874	10.35621133484516	4.806331896375177	47.746622416600786
2	30.984150914482246	7.55956680772556	30.2482120797075	31.2080701980847
3	28.422431280594175	11.639397646514821	13.969215420175535	45.968955652715465
4	37.90884257911711	12.148246859980734	18.361227744312732	31.581682816589424
5	34.424297658856176	17.44668972839218	23.089281130158934	25.039731482592703
6	30.081432293166664	26.6605578152462	18.366306959038102	24.891702932549027
7	31.516504608378902	12.369689130825686	38.78014622557195	17.333660035223467
8	21.44858938862283	20.39068341665545	27.335204524016067	30.825522670705656
9	32.44821432409209	13.630007786356163	30.680052044308948	23.2417258452428
10-11	29.005269501731707	23.374749735210056	23.41612431087704	24.203856452181196
12-13	29.020086659012023	17.553439045518772	24.84379078681174	28.582683508657457
14-15	30.537750679436744	17.55286504753404	25.980950875717358	25.928433397311863
16-17	32.43751714902409	19.40063464153926	22.91698300518781	25.24486520424884
18-19	29.404531820879793	19.209580079760357	24.637715498699794	26.74817260066006
20-21	31.270278898278946	18.52794412409943	22.64353303501902	27.558243942602605
22-23	30.14000011079496	18.222957636746177	22.1972062183204	29.439836034138462
24-25	29.635769578787595	18.083906624944575	26.512466335185053	25.767857461082773
26-27	29.98880169978157	16.611191318801083	21.16454379741449	32.23546318400286
28-29	37.27904998794938	16.317921732171072	17.022447526072025	29.38058075380753
30-31	32.096598349731856	14.353597812875613	21.438478399820646	32.11132543757188
32-33	34.92049226868098	15.218245046547565	20.32619638032866	29.535066304442793
34-35	33.43694775637203	14.947354706926975	20.88771658328901	30.727980953411986
36-37	34.2274005407006	13.253732321532876	16.977125162901483	35.54174197486504
38-39	33.71165251283303	16.050231765035125	18.725746499396536	31.512369222735316
40-41	34.70506615293402	16.04413470504613	21.250473298047588	28.000325843972263
42-43	31.50833455085425	16.442959851310494	21.183886194620957	30.864819403214295
44-45	26.85699193953318	18.716615926802174	22.829735311508372	31.596656822156273
46-47	30.587721876084377	14.57086208690583	23.1791232127388	31.66229282427098
48-49	29.936767371619354	18.411050862756717	19.262848209454518	32.38933355616941
50-51	26.504887559468028	21.752077455555035	19.885526113537736	31.8575088714392
52-53	31.15296972874791	15.729797390057861	20.260416878717763	32.85681600247647
54-55	31.218070050978458	18.53025472276079	18.61229320432504	31.63938202193571
56-57	28.131844800823032	15.169495264216279	20.7399187766154	35.95874115834528
58-59	29.302500155930822	15.751936470595851	17.565900003173677	37.379663370299646
60-61	30.1182115477582	16.007642449446962	17.88113516244243	35.993010840352405
62-63	29.034456631815868	16.426657641104555	20.305071919611446	34.233813807468124
64-65	27.43661643939572	15.384817926838085	19.94313949265653	37.23542614110966
66-67	25.727782772450496	16.113135677781536	20.42626027606635	37.73282127370161
68-69	29.17208266024839	17.409078552358395	21.361118084000065	32.05772070339315
70-71	33.733237666964236	16.547420762383094	19.79681798082779	29.922523589824877
72-73	30.863321164777417	17.526434822248866	19.108265885672086	32.501978127301626
74-75	31.92890560821095	19.93050306850671	17.43033153541738	30.710259787864963
76-77	27.31360886319653	21.70766642605109	20.779234041530714	30.199490669221664
78-79	29.171475438164002	19.569255770534138	20.827141290897806	30.43212750040405
80-81	26.198589896202822	23.884349730036572	22.718366022952647	27.198694350807955
82-83	28.151859519025557	19.305434896944345	23.468531863395526	29.074173720634573
84-85	29.558709593537262	19.733000111862886	20.021721155994797	30.68656913860506
86-87	31.24477871356713	20.278209196659162	18.94927551829752	29.527736571476186
88-89	27.438919085201835	23.28646375959108	18.21256990314535	31.06204725206173
90-91	28.078179326448073	18.808268721294677	23.058994111247884	30.054557841009366
92-93	34.23270919506727	16.013966438871783	20.350551248308793	29.40277311775215
94-95	31.979266525351925	17.67755276092697	18.83516653383508	31.50801417988603
96-97	31.41604435162289	14.497950994054381	23.371385840043715	30.714618814279014
98-99	29.530314135080815	18.34033355022893	21.803720588193087	30.32563172649717
100-101	28.359218083926645	16.259774401438573	20.19462402831652	35.186383486318256
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19329.0
1	18292.0
2	17233.5
3	17987.0
4	19032.5
5	18945.5
6	17844.5
7	16451.5
8	14942.0
9	13207.0
10	11645.0
11	10176.0
12	8996.0
13	8104.0
14	7263.5
15	6586.0
16	5978.0
17	5421.0
18	4889.5
19	4350.5
20	3948.0
21	3619.5
22	3246.0
23	2961.0
24	2656.0
25	2386.0
26	2171.5
27	1967.5
28	1901.0
29	1817.5
30	1690.5
31	1580.5
32	1457.5
33	1384.5
34	1317.0
35	1268.5
36	1343.5
37	1373.0
38	1283.5
39	1248.5
40	1285.5
41	1337.0
42	1456.0
43	1803.5
44	2226.5
45	2832.0
46	5648.5
47	6632.0
48	6083.5
49	10374.5
50	35569.5
51	40322.0
52	30667.5
53	50457.0
54	111497.5
55	178841.5
56	209448.5
57	325231.5
58	594507.5
59	1023519.0
60	2215809.5
61	2164585.5
62	1189749.0
63	1034589.5
64	951307.5
65	907464.5
66	657455.0
67	524843.0
68	495380.0
69	429532.5
70	361529.0
71	302690.0
72	235019.5
73	175067.0
74	133518.5
75	97186.0
76	66658.0
77	45715.0
78	31228.0
79	20210.0
80	11905.0
81	6332.0
82	3505.5
83	2000.0
84	1119.0
85	531.5
86	213.5
87	80.0
88	19.0
89	7.5
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.5
96	1.5
97	1.0
98	0.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.27286929778888636
2	0.0
3	0.0
4	0.0
5	0.005399585693594135
6	0.0
7	0.003824428433163708
8	9.34415323984152E-5
9	8.676713722709983E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	2.369410285816957E-4
32-33	0.0
34-35	0.0
36-37	2.1691784306774957E-4
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	1.201391130836767E-4
50-51	0.0
52-53	0.0
54-55	1.868830647968304E-4
56-57	0.0
58-59	1.7019707686854196E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	6.674395171315371E-6
68-69	0.0
70-71	4.004637102789223E-5
72-73	1.7353427445419966E-4
74-75	6.607651219602218E-4
76-77	0.001508413308717274
78-79	1.8354586721117274E-4
80-81	3.0702217788050715E-4
82-83	3.136965730518225E-4
84-85	2.0023185513946115E-5
86-87	6.173815533466719E-4
88-89	1.7019707686854196E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.4982631E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.472658343309991
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.73018628281501	9.512905289133023
2	12.81886073880436	3.710459836463716
3	5.894107878071588	2.559102286738257
4	3.416250387115599	1.9776889867169825
5	2.210361071396283	1.5994900300835513
6	1.5489149947366165	1.3450150512991705
7	1.1675239184319337	1.1828022345375528
8	0.947053139745355	1.0965101219594826
9	0.7336980872478892	0.955670556949087
>10	4.834577782337997	13.37224981936386
>50	0.4217990951087532	4.196132930438302
>100	0.22944389913682914	6.191368545248456
>500	0.019343689927850512	1.9580374906880809
>1k	0.019204526690959504	6.048771927097556
>5k	0.004174897106730327	4.293510196704148
>10k+	0.0044996113261426855	40.00028469657877
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACA	558861	3.7300591598364803	No Hit
ATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGA	503410	3.3599572731918714	No Hit
GGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCA	473398	3.159645325310354	No Hit
GTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGC	360156	2.403823467320259	No Hit
CCGCTATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGG	322623	2.1533133933552793	No Hit
CGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATT	296017	1.9757344354272626	No Hit
GCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT	267483	1.7852872436089497	No Hit
CCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTA	219854	1.4673924759943697	No Hit
CGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACC	117517	0.7843548973474685	No Hit
GAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTC	98765	0.6591966390949627	No Hit
ATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCA	96075	0.6412425160841243	No Hit
GGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGC	93821	0.6261984293679795	No Hit
ATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGC	92313	0.6161334414496359	No Hit
CTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGGGCTCTC	85679	0.5718555038831298	No Hit
CCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACT	85428	0.5701802306951296	No Hit
GGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCT	84283	0.5625380482239735	No Hit
CGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGGGCTCTCAC	77612	0.5180131580361286	No Hit
CATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGG	75558	0.5043039503542469	No Hit
GAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCAC	67616	0.45129590390366014	No Hit
CCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTAC	66462	0.4435936518759623	No Hit
CCACGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTT	65038	0.43408931315200916	No Hit
GGCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTGA	58546	0.3907591396998297	No Hit
CACGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTT	56426	0.37660942193664115	No Hit
CGGCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTG	55156	0.36813294006907066	No Hit
ATCCGCTATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGC	54259	0.3621460076004008	No Hit
GGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCG	54238	0.36200584530180313	No Hit
GGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAG	46038	0.3072758048970171	No Hit
CGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTC	43542	0.29061651454941395	No Hit
CGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGAT	42657	0.2847096748227998	No Hit
CTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCG	42335	0.2825605195776363	No Hit
TCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGAT	41994	0.2802845508242177	No Hit
CGCTATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGA	40784	0.27220853266692613	No Hit
CGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCG	40319	0.2691049389122645	No Hit
ACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTAC	39780	0.2655074399149255	No Hit
TATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTC	38864	0.2593936939380006	No Hit
GGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGATA	38524	0.25712439957975336	No Hit
CCGGGCTCTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACC	37830	0.2524923693308605	No Hit
CTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAA	36782	0.24549760319132202	No Hit
CCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGA	35588	0.23752837535677146	No Hit
TCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCG	33256	0.22196368581726403	No Hit
CTATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATT	33179	0.22144975738907272	No Hit
GCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCG	32388	0.21617031080856225	No Hit
GTCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT	30746	0.2052109539372624	No Hit
GTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAG	28244	0.18851161721863136	No Hit
CTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGC	27999	0.1868763904016591	No Hit
CCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTAT	27998	0.18686971600648777	No Hit
CACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACACTT	26565	0.17730530772599287	No Hit
GTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAAT	25986	0.17344083292180124	No Hit
GTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACACTTTCTG	25843	0.17248639441230315	No Hit
CTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACAC	25458	0.16991675227134673	No Hit
CTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAG	24985	0.16675976335531456	No Hit
GTCGGTTTGGGGTACGGGCCGTGTGTGTGCTCGCTAGAGGCTTTTCTTGG	24667	0.1646373056908363	No Hit
CTTCGCTTGACTACTACCAGCGAAGTTCCCACGCAGCCCCAGAACTCCAC	23943	0.15980504358680395	No Hit
CCCGTGTTCTGCGGGGGGCCTTATCCCACCAAGTGGGTGAGAAACCTGAT	23562	0.1572620990265328	No Hit
GGGGTGTGGACGCACGGCTAGCGCCGTGAACGGATGTGGTTGCGAGTTTG	22486	0.15008044982219745	No Hit
GCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTG	22480	0.15004040345116954	No Hit
CCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT	22212	0.14825166554525704	No Hit
GCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTGAC	21791	0.14544174517813327	No Hit
CCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATG	21216	0.14160396795462693	No Hit
GTTGCGAGTTTGTTTTTGCCTCCCTTTTCCAAAAGGGAGGGTGTTGTAAG	20149	0.13448238830683343	No Hit
CCGCTAACCACGACACTTTCTGACTGCCTCTCAGCCGGGTAGCGCTGAGA	19054	0.1271739255942431	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18805	0.12551200119658557	No Hit
GTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGG	17955	0.1198387653009675	No Hit
CTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGG	17787	0.11871746691218651	No Hit
AGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCC	17115	0.1142322733570626	No Hit
GCCGTGTGTGTGCTCGCTAGAGGCTTTTCTTGGCAGCAGAGGATCACCGA	16839	0.11239014028977955	No Hit
AGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACG	16214	0.10821864330770743	No Hit
CTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGAT	15982	0.10667018362796228	No Hit
CCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCC	15624	0.10428075015663137	No Hit
CTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTG	15461	0.10319282374370697	No Hit
CTCTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGAC	15402	0.10279903442859935	No Hit
GCCACTTCCGCTAACCACGACACTTTCTGACTGCCTCTCAGCCGGGTAGC	15298	0.10210489733078255	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.674395171315372E-6	1.3348790342630744E-5	0.0	0.0	0.0
2	6.674395171315372E-6	1.3348790342630744E-5	6.674395171315372E-6	0.0	0.0
3	2.6697580685261488E-5	6.674395171315371E-5	6.674395171315372E-6	0.0	2.0023185513946115E-5
4	5.3395161370522975E-5	8.009274205578446E-5	6.674395171315372E-6	0.0	3.337197585657686E-5
5	8.676713722709983E-5	8.009274205578446E-5	6.674395171315372E-6	0.0	3.337197585657686E-5
6	1.201391130836767E-4	1.5351108894025355E-4	6.674395171315372E-6	0.0	3.337197585657686E-5
7	1.3348790342630743E-4	1.6018548411156892E-4	6.674395171315372E-6	0.0	4.004637102789223E-5
8	1.868830647968304E-4	1.9355745996814578E-4	6.674395171315372E-6	0.0	4.004637102789223E-5
9	2.0690625031077653E-4	2.2025504065340727E-4	6.674395171315372E-6	0.0	4.004637102789223E-5
10-11	2.7698739960958796E-4	2.3694102858169572E-4	6.674395171315372E-6	0.0	4.004637102789223E-5
12-13	3.4373135132274163E-4	2.402782261673534E-4	1.0011592756973057E-5	0.0	4.3383568613549916E-5
14-15	4.57196069235103E-4	2.402782261673534E-4	1.3348790342630744E-5	0.0	4.67207661992076E-5
16-17	6.173815533466718E-4	2.4361542375301107E-4	1.3348790342630744E-5	0.0	1.401622985976228E-4
18-19	8.276250012431061E-4	2.7031300443827256E-4	1.3348790342630744E-5	0.0	5.806723799044373E-4
20-21	0.0011079495984383518	3.5708014166537243E-4	1.3348790342630744E-5	0.0	8.443109891713946E-4
22-23	0.0015284364942312202	3.604173392510301E-4	3.337197585657686E-5	0.0	8.543225819283676E-4
24-25	0.0020123301441515844	3.604173392510301E-4	6.006955654183835E-5	0.0	8.643341746853407E-4
26-27	0.002666420870940491	3.604173392510301E-4	6.006955654183835E-5	0.0	8.710085698566559E-4
28-29	0.0035541154287254354	3.604173392510301E-4	7.00811492988114E-5	0.0	9.410897191554674E-4
30-31	0.004648716236821156	3.604173392510301E-4	7.341834688446909E-5	0.0	0.0010211824612112517
32-33	0.006621000009944849	3.604173392510301E-4	7.341834688446909E-5	0.0	0.0011146239936096671
34-35	0.009427583179482963	3.704289320080031E-4	7.341834688446909E-5	0.0	0.001264797884964263
36-37	0.012401026228303962	3.8044052476497617E-4	7.341834688446909E-5	0.0	0.0013315418366774167
38-39	0.016545825629690807	3.937893151076069E-4	7.341834688446909E-5	0.0	0.001411634578733201
40-41	0.022749675941428446	4.3717288372115685E-4	7.341834688446909E-5	0.0	0.0016552500024862122
42-43	0.031389680490696195	4.538588716494453E-4	7.341834688446909E-5	0.0	0.001742017139713312
44-45	0.039108618506322426	4.538588716494453E-4	7.675554447012677E-5	0.0	0.001742017139713312
46-47	0.04702778837708811	4.6720766199207604E-4	8.009274205578446E-5	0.0	0.001742017139713312
48-49	0.05527066641366259	4.738820571633914E-4	9.677872998407289E-5	0.0	0.0017987494986694927
50-51	0.06492184183138462	4.738820571633914E-4	1.1346471791236132E-4	0.0	0.0018354586721117273
52-53	0.07742965838242963	4.8723084750602213E-4	1.1346471791236132E-4	0.0	0.0018654934503826463
54-55	0.0927941160667976	4.905680450916798E-4	1.1346471791236132E-4	0.0	0.0018888538334822502
56-57	0.11243686105597875	4.939052426773375E-4	1.1346471791236132E-4	0.0	0.0019088770189961963
58-59	0.13829680514724016	4.972424402629953E-4	1.1346471791236132E-4	0.0	0.0019188886117531695
60-61	0.165107850550414	5.606491943904912E-4	1.1346471791236132E-4	0.0	0.0019222258093388271
62-63	0.20008168124810655	7.44195061601664E-4	1.1346471791236132E-4	0.0	0.0019355745996814577
64-65	0.23910686981478751	8.910317553706021E-4	1.1346471791236132E-4	0.0	0.0019455861924384309
66-67	0.2783623250148789	9.210665336415213E-4	1.1346471791236132E-4	0.0	0.001968946575538035
68-69	0.3189760196323329	9.711244974263866E-4	1.1346471791236132E-4	0.0	0.001995644156223296
70-71	0.36941108674437756	0.001074577622581775	1.1346471791236132E-4	0.0	0.0020223417369085576
72-73	0.4340692899664952	0.0015150877038885894	1.1346471791236132E-4	0.0	0.002082411293450396
74-75	0.5138149634733713	0.0017720519179842313	1.201391130836767E-4	0.0	0.0020990972813786845
76-77	0.604560040222575	0.0017820635107412043	1.201391130836767E-4	0.0	0.002102434478964342
78-79	0.7113036421974218	0.0017887379059125196	1.201391130836767E-4	0.0	0.002115783269306973
80-81	0.8341525597206525	0.0017887379059125196	1.201391130836767E-4	0.0	0.002172515628263153
82-83	0.9802884419966027	0.001795412301083835	1.201391130836767E-4	0.0	0.002359398693059984
84-85	1.1593124064792093	0.0018020866962551504	1.201391130836767E-4	0.0	0.002419468249601822
86-87	1.353610724311371	0.0018321214745260696	1.201391130836767E-4	0.0	0.002452840225458399
88-89	1.5952338411057445	0.0018721678455539616	1.201391130836767E-4	0.0	0.0024595146206297146
90	1.7997106115741621	0.0018821794383109347	1.201391130836767E-4	0.0	0.0024695262133866878
>>END_MODULE