FastQCFastQC Report
Thu 25 Jan 2024
SRR25787974_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR25787974_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11072537
Sequences flagged as poor quality0
Sequence length101
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCC1667991.506420795884448No Hit
GGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCG1510741.3644027561163263No Hit
GGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTC1495171.3503409381246592No Hit
GTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGA1367711.2352273015660278No Hit
GTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAG1210891.09359761001476No Hit
GGCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTT1130441.0209403680475395No Hit
TATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTA887110.801180434077574No Hit
GTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAG813760.734935453365385No Hit
GGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCT769660.6951071827531486No Hit
CAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGAC764680.6906095685207464No Hit
CATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGT743650.6716166313104215No Hit
CGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTA715420.6461211193062619No Hit
GCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTG685600.6191896220351307No Hit
CTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGA635810.574222511064989No Hit
GGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTC616230.5565391201673112No Hit
GTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAG611020.5518337847956616No Hit
GGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGAT609080.5500817021428784No Hit
GGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACC605500.5468484774537218No Hit
GGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTG533450.48177757274597505No Hit
GTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCG530160.4788062573193479No Hit
TGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCT499050.4507097153976546No Hit
GTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAG490880.4433310992774285No Hit
CGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATAT473760.4278694214343109No Hit
CGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCG456670.41243483765283423No Hit
GTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCG424440.3833267840965444No Hit
CGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGA423250.3822520529847857No Hit
CCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCT422810.3818546734140513No Hit
CTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAG392690.3546522355265103No Hit
CTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGG390820.3529633723508894No Hit
GATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGT388860.35119322699034555No Hit
AGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACG386830.3493598621526395No Hit
GGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGG384530.3472826507601645No Hit
GCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTAGC370900.3349729154212806No Hit
GTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAA354540.3201976204730677No Hit
GTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGT347100.31347829318610543No Hit
GGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACC342490.30931483904727525No Hit
CTTCTCCAAGATCAGGTTTCTCACCCACTTGGTGGGATAAGGCCCCCCGC342110.3089716475998229No Hit
AGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTG341230.3081768884583542No Hit
GTTTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGA313590.283214226333134No Hit
GTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCC313130.28279878405463893No Hit
CCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGG308150.2783011698222368No Hit
GTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCT304880.27534791710337025No Hit
GAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGT300660.2715366857658728No Hit
GAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGG299980.2709225537020107No Hit
CTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGC291330.2631104325955289No Hit
CCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGG285590.25792643546822197No Hit
ATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTT275490.248804768049093No Hit
GTCAGTGGTAACACTGGGGCAAGCCGGTAGGGAGAGCGATAGGCAAATCC269220.2431421091661288No Hit
GGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCAC268740.24270860417987314No Hit
CAGGCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGG262320.23691047498870405No Hit
GCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCG260460.23523064316696346No Hit
GTTGTCCCGCCAGGGGCACCGCTGGATAGCCACGTTCGGTCAGGATAACC260070.23487842036563075No Hit
GGTGAGGCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGG254320.22968539188444348No Hit
CGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTAGCAGCGGGCCCG249720.22553096909949363No Hit
GGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTAC238120.21505459859831583No Hit
CGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCC234000.21133368079962164No Hit
GGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCC225420.20358477917030215No Hit
GCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATG221470.2000173943875735No Hit
ATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTC220560.1991955411844639No Hit
CAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGT219480.1982201549653887No Hit
CAGTTGTCCCGCCAGGGGCACCGCTGGATAGCCACGTTCGGTCAGGATAA217500.1964319468970842No Hit
GTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTG215600.19471598965982231No Hit
GAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCGAGTA214620.19383091697955038No Hit
CCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGG211880.19135632601634114No Hit
CGTGTGTGGGCGCCCGTGACGAATCAGCGGTACTAACCACCCAAAACCGG210330.18995646616489067No Hit
GTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAG204530.18471828091430176No Hit
GCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATG199580.1802477607435405No Hit
CCTTCTCCAAGATCAGGTTTCTCACCCACTTGGTGGGATAAGGCCCCCCG196430.17740288427123793No Hit
GTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAG195600.1766532818991709No Hit
TGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGT189190.1708641840618821No Hit
GGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCA184390.16652913419932577No Hit
GAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTG182340.164677706653859No Hit
GTGAGGCCAAGGCTCGATCCAGAAGAGAAGGTTCGGTCTCCCGACCCGGG181680.16408163729775752No Hit
AACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCG180250.16279015369287095No Hit
AAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGT176840.15971046201967987No Hit
CTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGT175790.15876216986224564No Hit
GGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAA170310.15381298793582718No Hit
GGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGG167720.15147386728082282No Hit
GTTGGCTGGGCGGCCGCGGCTCGCGTAGGGCTTGTGTGGATTCACGAGGT166500.15037204210742308No Hit
CGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCC162320.14659693618544692No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA159440.14399590626791312No Hit
GCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCG155250.14021176899205665No Hit
GCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGC148290.13392594669134997No Hit
ACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGC147540.13324859515032553No Hit
TGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGA142290.12850713436315453No Hit
CCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAG140030.12646604838620093No Hit
GAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTC134290.12128205125889396No Hit
CGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGG132180.11937643559014524No Hit
TGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGC131580.11883455435732569No Hit
AGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGC129030.11653155911784265No Hit
GGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGA125920.11372280806106133No Hit
GGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCC125670.1134970242140532No Hit
GGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGC124420.11236810497901248No Hit
ATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAG124240.11220554060916663No Hit
GTTCGGTCTCCCGACCCGGGCGCCCAGCATGGTTCCCGGCACCCACGCGG120350.10869234394971994No Hit
GGCAGAAGTGTTGCGGGTCTGCGTAATTGCGAACAGCAGATGGCATCGAC119400.10783436533108898No Hit
GTTGCGGGTCTGCGTAATTGCGAACAGCAGATGGCATCGACGGCCCTTTG113150.10218976915588542No Hit
AGGCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGT111580.10077184659667428No Hit
CCTGCCTAGTATCAATTCCCGAGTAGCAGCGGGCCCGTGGAATCCGCTGT111530.10072668982727265No Hit
ACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGA110740.10001321287072691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)