##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25787973_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14912526
Sequences flagged as poor quality	0
Sequence length	101
%GC	62
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.483273591610164	35.0	33.0	35.0	31.0	35.0
2	33.77123365954232	35.0	35.0	35.0	31.0	35.0
3	33.68815450849843	35.0	34.0	35.0	31.0	35.0
4	33.63786222401222	35.0	34.0	35.0	31.0	35.0
5	33.724783782439005	35.0	34.0	35.0	31.0	35.0
6	37.96065247430248	40.0	39.0	40.0	34.0	40.0
7	38.03632805066023	40.0	39.0	40.0	34.0	40.0
8	37.846934047256646	39.0	39.0	40.0	34.0	40.0
9	38.05375159111206	40.0	39.0	40.0	34.0	40.0
10-11	38.15538450695744	40.0	39.0	40.0	35.0	40.0
12-13	38.15355386471749	40.0	39.0	40.0	34.5	40.0
14-15	38.169295027549325	40.0	39.0	40.0	35.0	40.0
16-17	38.12741312236438	40.0	39.0	40.0	34.0	40.0
18-19	38.127421705752596	40.0	39.0	40.0	34.0	40.0
20-21	38.1591308206269	40.0	39.0	40.0	34.5	40.0
22-23	38.10984011025362	40.0	39.0	40.0	34.0	40.0
24-25	38.09702645279546	40.0	39.0	40.0	34.0	40.0
26-27	38.096339178218365	40.0	39.0	40.0	34.0	40.0
28-29	38.084687765171374	40.0	39.0	40.0	34.0	40.0
30-31	38.09641679752981	40.0	39.0	40.0	34.0	40.0
32-33	38.073668404668666	40.0	39.0	40.0	34.0	40.0
34-35	38.04758533195516	40.0	39.0	40.0	34.0	40.0
36-37	38.02479861560677	40.0	39.0	40.0	34.0	40.0
38-39	38.05243521453039	40.0	39.0	40.0	34.0	40.0
40-41	38.02765698447064	40.0	39.0	40.0	34.0	40.0
42-43	37.978441747561746	40.0	39.0	40.0	34.0	40.0
44-45	37.93819853189191	40.0	39.0	40.0	34.0	40.0
46-47	37.921990110863845	40.0	39.0	40.0	34.0	40.0
48-49	37.89027697252632	40.0	39.0	40.0	34.0	40.0
50-51	37.88219316432374	40.0	39.0	40.0	34.0	40.0
52-53	37.857329200968366	40.0	39.0	40.0	34.0	40.0
54-55	37.85486368975987	40.0	39.0	40.0	34.0	40.0
56-57	37.85927923947962	40.0	39.0	40.0	34.0	40.0
58-59	37.842519134585245	40.0	39.0	40.0	34.0	40.0
60-61	37.80150425219711	40.0	39.0	40.0	34.0	40.0
62-63	37.78834186106364	40.0	39.0	40.0	34.0	40.0
64-65	37.774851155330765	40.0	39.0	40.0	34.0	40.0
66-67	37.75001052806212	40.0	39.0	40.0	34.0	40.0
68-69	37.710286842081615	40.0	39.0	40.0	34.0	40.0
70-71	37.70422096833226	40.0	39.0	40.0	34.0	40.0
72-73	37.67027544495144	40.0	38.0	40.0	34.0	40.0
74-75	37.66249104947076	40.0	38.0	40.0	34.0	40.0
76-77	37.66208967548489	40.0	38.0	40.0	34.0	40.0
78-79	37.5874634518659	39.0	38.0	40.0	34.0	40.0
80-81	37.53186224117899	39.0	38.0	40.0	34.0	40.0
82-83	37.4907524721164	39.0	38.0	40.0	34.0	40.0
84-85	37.484009851852065	39.0	38.0	40.0	34.0	40.0
86-87	37.47875859529097	39.0	38.0	40.0	34.0	40.0
88-89	37.42789524725724	39.0	38.0	40.0	34.0	40.0
90-91	37.37804175496492	39.0	38.0	40.0	34.0	40.0
92-93	37.34481193863468	39.0	38.0	40.0	34.0	40.0
94-95	37.302976269748	39.0	38.0	40.0	34.0	40.0
96-97	37.252759525783894	39.0	38.0	40.0	32.5	40.0
98-99	37.183148381434506	39.0	38.0	40.0	31.0	40.0
100-101	35.79770828228564	38.5	36.0	39.5	29.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0895966381221669
1101	2	0.2538106576798711
1101	3	0.1888644123497727
1101	4	0.15352531747434028
1101	5	0.1698736963020835
1101	6	0.31073199721232214
1101	7	0.37382186402225415
1101	8	0.3218893340820088
1101	9	0.310945631515672
1101	10-11	0.3646401417899128
1101	12-13	0.3741607409396437
1101	14-15	0.3747324455779619
1101	16-17	0.3941188954290382
1101	18-19	0.38136401754094607
1101	20-21	0.396316432190126
1101	22-23	0.3884089097991321
1101	24-25	0.39186487718299645
1101	26-27	0.38443437421456395
1101	28-29	0.39357090277240303
1101	30-31	0.4308894840233535
1101	32-33	0.39496726529369397
1101	34-35	0.32783718845841037
1101	36-37	0.37336055808965085
1101	38-39	0.39809717865366423
1101	40-41	0.4274793278862603
1101	42-43	0.42032590153679195
1101	44-45	0.37859939292209077
1101	46-47	0.3628036925148166
1101	48-49	0.34788797020945594
1101	50-51	0.3754989321021043
1101	52-53	0.3696199185224458
1101	54-55	0.3384390231711123
1101	56-57	0.31626567820431006
1101	58-59	0.34643937385733636
1101	60-61	0.3558210823513477
1101	62-63	0.3255307867082493
1101	64-65	0.32402438157222946
1101	66-67	0.3572261314280851
1101	68-69	0.3481471441280988
1101	70-71	0.3861177772716431
1101	72-73	0.31531799283335005
1101	74-75	0.2958084488363468
1101	76-77	0.30718432507629956
1101	78-79	0.3233688952540348
1101	80-81	0.3065240424536384
1101	82-83	0.37397545598422965
1101	84-85	0.41504559038603617
1101	86-87	0.35311082412526673
1101	88-89	0.3683005749401502
1101	90-91	0.3864773799112484
1101	92-93	0.3658882647097883
1101	94-95	0.3746531876932053
1101	96-97	0.3405823536499284
1101	98-99	0.2890758919417067
1101	100-101	0.3232923025708274
1102	1	0.03970474827343651
1102	2	0.22232383924177412
1102	3	0.23768869040640794
1102	4	0.20229613459152773
1102	5	0.25575050210827754
1102	6	0.2185634816315769
1102	7	0.33931898398954985
1102	8	0.23378957624059638
1102	9	0.3739954083581196
1102	10-11	0.3998465243289644
1102	12-13	0.4075734320053499
1102	14-15	0.4145797899250354
1102	16-17	0.41266058421077645
1102	18-19	0.4081578278415279
1102	20-21	0.39190834913147654
1102	22-23	0.3699435646874889
1102	24-25	0.37180697549996466
1102	26-27	0.41106593441165273
1102	28-29	0.44566443497198094
1102	30-31	0.44922355333109465
1102	32-33	0.4370251470590105
1102	34-35	0.41325007010036785
1102	36-37	0.3777059116844228
1102	38-39	0.40627316020624704
1102	40-41	0.401682545999428
1102	42-43	0.4233471688960364
1102	44-45	0.3897225049553512
1102	46-47	0.40435607190007516
1102	48-49	0.4058241923498471
1102	50-51	0.389960048322159
1102	52-53	0.3742179667228527
1102	54-55	0.44538972616435757
1102	56-57	0.43312081241231937
1102	58-59	0.346455013785004
1102	60-61	0.3624030361381898
1102	62-63	0.3765541381840549
1102	64-65	0.3967580802295174
1102	66-67	0.3697378811783807
1102	68-69	0.2943897476984887
1102	70-71	0.2681211659980818
1102	72-73	0.3149130182951936
1102	74-75	0.37202790486110615
1102	76-77	0.36808076001023693
1102	78-79	0.33927484881544956
1102	80-81	0.2866917088157237
1102	82-83	0.32606865999429857
1102	84-85	0.37492485286046673
1102	86-87	0.31410796797118223
1102	88-89	0.37629071069518005
1102	90-91	0.348975300434617
1102	92-93	0.34739637019848857
1102	94-95	0.35434640519299876
1102	96-97	0.36437509722143346
1102	98-99	0.38860191838834623
1102	100-101	0.37328297746199723
1103	1	0.12139239273358982
1103	2	0.23018853935264616
1103	3	0.2225101267920806
1103	4	0.2240902405835783
1103	5	0.23504089986758459
1103	6	0.2685341234995704
1103	7	0.30105152705268523
1103	8	0.2186567898992351
1103	9	0.32547230254073867
1103	10-11	0.2723031064456123
1103	12-13	0.34748685868865437
1103	14-15	0.3481187503498546
1103	16-17	0.36065787032364227
1103	18-19	0.3780085692611337
1103	20-21	0.3649281215733211
1103	22-23	0.3793469655746833
1103	24-25	0.40000729040956173
1103	26-27	0.37132066357704474
1103	28-29	0.32818049735685406
1103	30-31	0.37528414937555254
1103	32-33	0.3506810837376051
1103	34-35	0.3961289161247876
1103	36-37	0.3757593275661151
1103	38-39	0.3545573952661485
1103	40-41	0.3741237969970257
1103	42-43	0.3473949960804603
1103	44-45	0.31146111640094176
1103	46-47	0.3125789321003154
1103	48-49	0.3557354490700959
1103	50-51	0.3354083542839916
1103	52-53	0.40605228782877134
1103	54-55	0.37299566621668845
1103	56-57	0.337412787369054
1103	58-59	0.32164874303481383
1103	60-61	0.3208895092787003
1103	62-63	0.3064865731095381
1103	64-65	0.3156277538166563
1103	66-67	0.36828068323264773
1103	68-69	0.33077815931398646
1103	70-71	0.2555018427333735
1103	72-73	0.33617518147341485
1103	74-75	0.3418539247290724
1103	76-77	0.34199267132441946
1103	78-79	0.26733281744988346
1103	80-81	0.2796273031092511
1103	82-83	0.3203480927296454
1103	84-85	0.35843905155719114
1103	86-87	0.3020357318731257
1103	88-89	0.34464061819268466
1103	90-91	0.35703342195676413
1103	92-93	0.32073737947931846
1103	94-95	0.32952543420272207
1103	96-97	0.322425332213534
1103	98-99	0.2680665940198139
1103	100-101	0.3060068259705204
1104	1	-0.005444995788984386
1104	2	0.16504258870370592
1104	3	0.14092070607787832
1104	4	0.11053963056008342
1104	5	0.1400283877379067
1104	6	0.14797185700972904
1104	7	0.25595972594367566
1104	8	0.16709000024525977
1104	9	0.23425533034343715
1104	10-11	0.25030812150539816
1104	12-13	0.2501714317180159
1104	14-15	0.30445462902922316
1104	16-17	0.32657361152585196
1104	18-19	0.2850666147278247
1104	20-21	0.25077834595300175
1104	22-23	0.27709787560396393
1104	24-25	0.28920618859321223
1104	26-27	0.30939528457823684
1104	28-29	0.28682833542423225
1104	30-31	0.3015743640854751
1104	32-33	0.32746705214014327
1104	34-35	0.2993365788898714
1104	36-37	0.2872079633654252
1104	38-39	0.269892681842812
1104	40-41	0.3046759739919196
1104	42-43	0.28929321949316744
1104	44-45	0.2726101788038804
1104	46-47	0.28070891356101413
1104	48-49	0.2722403823807724
1104	50-51	0.2950299348575882
1104	52-53	0.28388689655517396
1104	54-55	0.2867285884375832
1104	56-57	0.26058215394735385
1104	58-59	0.2601410508066664
1104	60-61	0.23499127833609634
1104	62-63	0.2368077938657578
1104	64-65	0.2824758685893016
1104	66-67	0.29184647249400797
1104	68-69	0.30286394193920785
1104	70-71	0.26719478276224606
1104	72-73	0.276533463816115
1104	74-75	0.27433757435461104
1104	76-77	0.23668322547534615
1104	78-79	0.26519097592856866
1104	80-81	0.24195741866606113
1104	82-83	0.2906477116277273
1104	84-85	0.2515416212936117
1104	86-87	0.2494881667872093
1104	88-89	0.27850477420825825
1104	90-91	0.2715948635351495
1104	92-93	0.2654782345986533
1104	94-95	0.27623084160347133
1104	96-97	0.2936506254767721
1104	98-99	0.23869835980516285
1104	100-101	0.2863403448930484
1105	1	0.010804312005262773
1105	2	0.09304160597481115
1105	3	0.07671634988185616
1105	4	0.03120041277985308
1105	5	0.08678391720882672
1105	6	0.0899604190523462
1105	7	0.1417835263024685
1105	8	0.03560385295605073
1105	9	0.1258230514105989
1105	10-11	0.12935776612948047
1105	12-13	0.17704144313521653
1105	14-15	0.19846776818999246
1105	16-17	0.1412269073979573
1105	18-19	0.18642448331770822
1105	20-21	0.1939428169263948
1105	22-23	0.2180554093314555
1105	24-25	0.18667425632698098
1105	26-27	0.1859706026455612
1105	28-29	0.2108489545235841
1105	30-31	0.22177537318724916
1105	32-33	0.15207641546358985
1105	34-35	0.2058717119086637
1105	36-37	0.22750953061656531
1105	38-39	0.17267628159324744
1105	40-41	0.21872094248747942
1105	42-43	0.17326835594383994
1105	44-45	0.11580587181960311
1105	46-47	0.14462403882312458
1105	48-49	0.1529841270751291
1105	50-51	0.12153792163220345
1105	52-53	0.23683545703137554
1105	54-55	0.1432006066553697
1105	56-57	0.10541451253305922
1105	58-59	0.14188605146249245
1105	60-61	0.1786442259495331
1105	62-63	0.16254748251082418
1105	64-65	0.15157825136563474
1105	66-67	0.0942385851113201
1105	68-69	0.16842926869018982
1105	70-71	0.1538223533204217
1105	72-73	0.14412240554066358
1105	74-75	0.16436226832644962
1105	76-77	0.15282844417125574
1105	78-79	0.176030132063687
1105	80-81	0.17788107908008044
1105	82-83	0.1988085257653509
1105	84-85	0.19132520822983423
1105	86-87	0.1889472740956677
1105	88-89	0.22749969124125613
1105	90-91	0.18750711518168117
1105	92-93	0.24161658057000324
1105	94-95	0.20971673146311787
1105	96-97	0.14482617904555184
1105	98-99	0.11050945606667995
1105	100-101	0.24776850745921308
1106	1	0.05848311460383826
1106	2	0.1610414218001992
1106	3	0.11487823587697932
1106	4	0.02176191028747354
1106	5	0.08094283764165056
1106	6	0.060689115706075825
1106	7	0.11121501730220729
1106	8	-0.042961066454637376
1106	9	0.07796856392121754
1106	10-11	0.1312937608912108
1106	12-13	0.09116228356180045
1106	14-15	0.19068510624851598
1106	16-17	0.18828639173538164
1106	18-19	0.15836287699276141
1106	20-21	0.14298425547503513
1106	22-23	0.1729882825353144
1106	24-25	0.1792491565808234
1106	26-27	0.19948930754007677
1106	28-29	0.158284764144895
1106	30-31	0.1746354184148302
1106	32-33	0.13754018841245141
1106	34-35	0.1518696691322745
1106	36-37	0.21374497264479686
1106	38-39	0.1500694303599701
1106	40-41	0.1857648051016696
1106	42-43	0.19335174232682562
1106	44-45	0.1334829400547548
1106	46-47	0.1586419756614248
1106	48-49	0.08368946947747702
1106	50-51	0.09944328353221721
1106	52-53	0.17844795980047934
1106	54-55	0.16870655207954854
1106	56-57	0.17078999580247256
1106	58-59	0.1445364842358856
1106	60-61	0.18140243335160733
1106	62-63	0.14487806665126612
1106	64-65	0.14931691952702408
1106	66-67	0.13207618516459974
1106	68-69	0.10761066677840603
1106	70-71	0.14979503679442985
1106	72-73	0.14535513751586393
1106	74-75	0.15008710158540595
1106	76-77	0.14113083494591905
1106	78-79	0.13158095545911408
1106	80-81	0.1673055991698007
1106	82-83	0.19332074759277873
1106	84-85	0.1403632270733155
1106	86-87	0.18523800796469203
1106	88-89	0.14552681294813397
1106	90-91	0.1781594622623217
1106	92-93	0.1831177089714302
1106	94-95	0.17535771606469552
1106	96-97	0.13853362057113117
1106	98-99	0.18492615966873416
1106	100-101	0.20571860049038548
1107	1	-0.037346351355338925
1107	2	0.02942556613276537
1107	3	0.049382225939673674
1107	4	-0.04700183597105223
1107	5	-0.05443232983066082
1107	6	-0.02553324049227257
1107	7	-0.01613974769175286
1107	8	-0.11169062928232165
1107	9	-0.012447381676395253
1107	10-11	0.017000675213850514
1107	12-13	0.020797232324710535
1107	14-15	0.07713372810007968
1107	16-17	0.0364062771261473
1107	18-19	0.03410987828911516
1107	20-21	0.04982642975836171
1107	22-23	0.10906261554949026
1107	24-25	0.06471089961993925
1107	26-27	-0.001655505523899592
1107	28-29	0.08193914365512
1107	30-31	0.07653445456590902
1107	32-33	0.01042424463603453
1107	34-35	0.03329058350195879
1107	36-37	0.09666751668745377
1107	38-39	0.08488640211878362
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2216	66-67	-0.8142553339201726
2216	68-69	-0.6344748737529429
2216	70-71	-0.7244276834459527
2216	72-73	-0.8192105049998091
2216	74-75	-0.845919919126473
2216	76-77	-0.8174674757908988
2216	78-79	-0.7149425176423847
2216	80-81	-0.7642842677010648
2216	82-83	-0.8471427097722071
2216	84-85	-0.8043631622614242
2216	86-87	-0.7812040999382575
2216	88-89	-0.7952802518541446
2216	90-91	-0.8397076354389341
2216	92-93	-0.8828970918958774
2216	94-95	-0.8677392368779238
2216	96-97	-0.8525173679794733
2216	98-99	-0.864691145824267
2216	100-101	-1.4869272336985802
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	1.0
8	6.0
9	19.0
10	130.0
11	108.0
12	29.0
13	26.0
14	33.0
15	75.0
16	602.0
17	4314.0
18	9054.0
19	9239.0
20	10185.0
21	14031.0
22	20786.0
23	31441.0
24	45016.0
25	61458.0
26	81640.0
27	104316.0
28	132049.0
29	162784.0
30	198502.0
31	241676.0
32	295285.0
33	361163.0
34	453962.0
35	582193.0
36	790436.0
37	1202614.0
38	2515835.0
39	7583516.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	36.40970740743374	12.655906059978747	7.148612745480142	43.78577378710737
2	36.693595764954324	16.85327376928508	25.96224212340306	20.49088834235754
3	29.129441531769146	18.451181779552805	22.08230233211938	30.33707435655867
4	32.84347589960309	23.617489631939296	18.7044315189582	24.83460294949942
5	37.32240705413097	22.626761376645188	19.317478411873594	20.73335315735025
6	30.990166769639043	29.22601552440217	16.01822017450548	23.765597531453302
7	31.62772641136044	16.549658800824957	26.084130050407	25.738484737407607
8	29.599811850684176	19.151388644019228	18.104455998939347	33.14434350635725
9	32.224804473020214	16.81952838808648	23.539822118649038	27.415845020244273
10-11	33.83490576634776	20.334072019745694	16.510811545772572	29.320210668133978
12-13	34.27462958163589	16.314325499119576	18.63476992235711	30.776274996887427
14-15	35.79706274997561	18.55093894908645	18.59491443642415	27.057083864513782
16-17	34.88252012633302	17.838505915561186	19.44723451793649	27.8317394401693
18-19	35.05962708074497	18.042290424243728	19.70992076936914	27.188161725642168
20-21	35.420605469522734	18.88143229389843	18.66393728332812	27.034024953250714
22-23	34.276312734357724	18.451912523325756	19.056129843337093	28.215644898979424
24-25	34.961816801586345	18.169571603769437	18.261997331167727	28.606614263476494
26-27	36.43654399152812	17.97755276584028	18.324535953113898	27.2613672895177
28-29	35.599203288186466	18.412698167880688	19.02666142624746	26.96143711768539
30-31	36.224238055322715	19.076455993294218	17.782293378038556	26.91701257334451
32-33	34.82878432677446	18.986300908714952	19.15388900478985	27.031025759720745
34-35	35.37963041788444	18.57272697919133	18.27451518705749	27.77312741586674
36-37	34.022362552581974	19.92674232167393	18.534987255598182	27.515907870145913
38-39	35.1427283345558	19.194410792645055	19.13859194612636	26.524268926672782
40-41	34.311473799879586	19.239449324020637	18.872464333449244	27.576612542650537
42-43	32.24742333302788	20.0431196132546	18.546241830410516	29.163215223307006
44-45	33.41473549444369	19.790482143343915	17.977438276873368	28.817344085339023
46-47	34.28627661819854	17.969686590887335	18.187348060361266	29.55668873055286
48-49	32.148506058922905	18.353372603825697	19.968598090631403	29.529523246619995
50-51	33.37001651313087	19.725181267236106	18.47331830107545	28.431483918557575
52-53	33.911397604298514	18.918920006370655	17.573156072658993	29.596526316671834
54-55	34.27913995485486	19.312437486337906	17.803803781449304	28.604618777357928
56-57	33.07163231032532	21.1517967752201	16.57201154886209	29.204559365592488
58-59	33.28682416277709	18.70116625109406	17.41420166218536	30.597807923943492
60-61	33.24108333365467	19.72879941937324	17.291461322817046	29.738655924155044
62-63	33.406353436390184	20.901033443424048	16.989901369040584	28.70271175114518
64-65	33.322793318171364	19.354820970521466	18.11214982979161	29.210235881515565
66-67	33.15413808928557	19.56773904821267	17.954240779739237	29.323882082762527
68-69	33.452276890380546	19.764471906668078	18.012944257421317	28.77030694553006
70-71	35.27168368035545	19.608255779434913	16.38331196835052	28.73674857185912
72-73	33.251938843178	21.034905631939196	17.21749811982785	28.495657405054953
74-75	32.27964038895227	19.435126470648985	18.178755860264104	30.10647728013464
76-77	32.4041124511523	20.07964510908735	17.51342714389173	30.00281529586862
78-79	32.462624217493044	19.991843997352067	17.697459382707763	29.848072402447123
80-81	33.084579183034485	20.468885634699305	17.276638319629257	29.169896862636957
82-83	33.71889319318011	20.697077892999133	16.90425475660431	28.679774157216453
84-85	32.28496785239801	20.199380856030917	19.039696437038383	28.475954854532688
86-87	34.35793930808566	20.326824230827096	17.03064988987637	28.284586571210873
88-89	33.65184054011633	19.2667688478291	17.35912896343055	29.722261648624016
90-91	33.15342365767863	18.939812665750253	19.420286375662883	28.486477300908238
92-93	34.043056042294914	20.389133207734737	17.648308219929817	27.91950253004053
94-95	33.39227263711721	20.899799327380386	17.223203314443047	28.484724721059358
96-97	32.239219179828176	20.867954873256956	18.204916237152506	28.68790970976236
98-99	32.708955976836094	19.20925070123416	18.036341820213455	30.045451501716293
100-101	34.758831681998984	19.549333318042425	16.618602577906426	29.073232422052158
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	42972.0
1	45261.0
2	42969.0
3	34490.5
4	27449.5
5	21975.5
6	17981.5
7	14931.5
8	12497.5
9	10505.5
10	8961.0
11	7867.0
12	7061.5
13	6437.5
14	5811.5
15	5213.0
16	4665.5
17	4213.0
18	3749.0
19	3328.0
20	3085.5
21	2820.0
22	2513.0
23	2316.5
24	2169.5
25	2016.0
26	1962.5
27	1872.5
28	1760.5
29	1705.0
30	1596.5
31	1513.5
32	1432.5
33	1346.5
34	1323.5
35	1273.0
36	1226.5
37	1228.0
38	1210.0
39	1241.0
40	1476.5
41	1701.5
42	1845.5
43	2201.5
44	3941.5
45	5629.0
46	5537.5
47	6094.0
48	7591.5
49	13257.0
50	20551.0
51	27762.5
52	39994.5
53	66653.5
54	146557.5
55	177217.5
56	196677.0
57	302869.0
58	461549.5
59	949540.5
60	1399947.5
61	1378828.5
62	1086424.5
63	964029.5
64	974107.5
65	883567.0
66	821520.0
67	770581.0
68	732804.5
69	652186.5
70	569259.5
71	488069.0
72	394827.0
73	302365.0
74	223557.5
75	163207.5
76	112698.0
77	75016.5
78	49886.0
79	31717.0
80	20787.0
81	13755.5
82	8739.5
83	5682.0
84	3418.0
85	1813.0
86	1103.0
87	612.0
88	328.5
89	221.0
90	131.5
91	70.5
92	47.0
93	37.5
94	27.0
95	13.0
96	8.5
97	6.5
98	5.5
99	3.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03562106111332178
2	0.010179361967248205
3	0.009750192556244328
4	0.01065547178258063
5	0.00885161910195496
6	0.026802970871601498
7	0.004747686609230388
8	0.006471070025292831
9	0.006041900614288954
10-11	0.006176016055227665
12-13	0.008345333312411324
14-15	0.013052785289360097
16-17	2.3805490766621296E-4
18-19	3.252299442763754E-4
20-21	0.0
22-23	6.269896863884764E-4
24-25	7.711637853975913E-5
26-27	8.851619101954961E-4
28-29	0.0015624448869359891
30-31	1.7435007322032497E-4
32-33	0.002474429885319228
34-35	0.0020486133603388186
36-37	6.437541165058153E-4
38-39	0.0
40-41	0.0013378015233636476
42-43	0.0019212036914470424
44-45	9.756898328291264E-4
46-47	9.958071489699332E-4
48-49	3.822290066753278E-4
50-51	0.001012571579087272
52-53	0.0026890145908211663
54-55	0.008620269966335683
56-57	0.0027326021091262473
58-59	0.005173503134210797
60-61	0.00706453085144663
62-63	0.012016743508108553
64-65	0.007369643479582198
66-67	0.0113662836195558
68-69	0.006514657543597913
70-71	0.00968648772179844
72-73	0.00665547875658356
74-75	0.007255645354784294
76-77	0.007557405096896394
78-79	0.013649599001537366
80-81	0.007711637853975912
82-83	0.007909458129360513
84-85	0.01083652762784789
86-87	0.008922029708447785
88-89	0.00520032622239854
90-91	0.005257325284797492
92-93	0.012046919482319764
94-95	0.009545666508812792
96-97	0.010658824668604098
98-99	0.007627815703389217
100-101	0.011436694226048625
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.4912526E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.014830665121675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.525871275512955	10.363188298919528
2	13.883099558086673	5.002033752919139
3	7.388581309982234	3.9931212346443847
4	4.48140139573911	3.2292674914672
5	3.0766133089454675	2.771233389135614
6	2.2415336387998925	2.4228509359892447
7	1.6729108963896546	2.1096044561407576
8	1.2842645544534228	1.8508646782157079
9	1.0526040613991905	1.7066235531173182
>10	6.691608878246423	22.36633199186457
>50	0.4545843656324021	5.586149795005963
>100	0.21497835493487133	6.931053687931297
>500	0.01347403090986304	1.675809625727401
>1k	0.012838754773743835	4.784024052138006
>5k	0.002164673769991693	2.638511911263644
>10k+	0.0034709424242970253	22.569331145520184
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCG	151298	1.0145698991572587	No Hit
GGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTC	150619	1.0100166799373895	No Hit
GTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGA	111025	0.7445083415110223	No Hit
GGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCC	105566	0.707901531906801	No Hit
GGCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTT	105456	0.7071638969816382	No Hit
TATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTA	100627	0.6747817237669863	No Hit
CGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATAT	97020	0.6505940039936896	No Hit
CATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGT	92229	0.6184666501168213	No Hit
CGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTA	88832	0.5956871424733812	No Hit
GTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAG	86921	0.5828724120916873	No Hit
CAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGAC	83885	0.5625136881571907	No Hit
GGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCT	67180	0.450493766113132	No Hit
CTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGA	65996	0.4425541320095603	No Hit
TGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCT	62736	0.4206933151365503	No Hit
GTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAG	58724	0.3937897576842448	No Hit
GGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTC	58389	0.3915433240485214	No Hit
GTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAG	58337	0.3911946239020807	No Hit
GGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACC	57727	0.38710410295345005	No Hit
CCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCT	57102	0.3829129954241153	No Hit
GCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTG	56402	0.3782189549912604	No Hit
CTTCTCCAAGATCAGGTTTCTCACCCACTTGGTGGGATAAGGCCCCCCGC	52226	0.3502156509232574	No Hit
CGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGA	49319	0.3307219715828157	No Hit
CTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGG	48761	0.32698015078062564	No Hit
GGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTG	48300	0.3238887898669883	No Hit
GTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAG	46561	0.31222745227736737	No Hit
GGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGAT	46364	0.31090641518412104	No Hit
GTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCG	42803	0.2870271609249835	No Hit
GGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGG	40587	0.27216717006897423	No Hit
GATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGT	38174	0.2559861421197187	No Hit
CTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAG	36692	0.24604818794616018	No Hit
GTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAA	36572	0.24524349530052786	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36157	0.24246059990104962	No Hit
GTTTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGA	36134	0.2423063671439701	No Hit
AGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTG	34639	0.23228123793380143	No Hit
GTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCC	33483	0.2245293654475439	No Hit
CGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCG	32864	0.22037849255049077	No Hit
ATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTT	31716	0.2126802662406087	No Hit
ATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTC	31275	0.20972302076791016	No Hit
CAGGCTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGG	30057	0.2015553904147426	No Hit
CCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGG	29606	0.19853108722157467	No Hit
AGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACG	28883	0.19368281403164023	No Hit
GAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGTCGT	26710	0.17911117137364924	No Hit
CCTTCTCCAAGATCAGGTTTCTCACCCACTTGGTGGGATAAGGCCCCCCG	26543	0.17799130744181102	No Hit
CCCGGCTGAGAGGCAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGG	24317	0.1630642588653324	No Hit
GTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTG	24210	0.1623467412563103	No Hit
CTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGC	23385	0.15681447931758843	No Hit
GGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTAC	22929	0.15375664726418584	No Hit
GAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGG	22854	0.15325371436066565	No Hit
CAGTTGTCCCGCCAGGGGCACCGCTGGATAGCCACGTTCGGTCAGGATAA	22083	0.14808356411247833	No Hit
GTTGTCCCGCCAGGGGCACCGCTGGATAGCCACGTTCGGTCAGGATAACC	21750	0.14585054202084877	No Hit
TGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAAAGTGT	21177	0.1420081346379547	No Hit
GCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATG	20233	0.13567788582564752	No Hit
GTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCTAGTATCAATTCCCG	20165	0.1352218933264559	No Hit
CCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGG	19359	0.12981704105662584	No Hit
CGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGG	19014	0.12750354970043304	No Hit
CTAAACCGCACGCATGGGTAACCGGGTAGGGGTTGTGTGTGCGGGGTTGT	18889	0.1266653281945661	No Hit
GTTAGCGGAAGTGGCCTGGGATGGTCTGCCGTAGACGGTGAGAGCCCGGT	18846	0.12637697999654787	No Hit
AACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCG	18163	0.12179693768849087	No Hit
GGATGGTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACC	17662	0.11843734589297615	No Hit
GGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAGGCAGTCAGAA	16977	0.11384389204082528	No Hit
CAGTCAGAAAGTGTCGTGGTTAGCGGAAGTGGCCTGGGATGGTCTGCCGT	16612	0.11139628524369379	No Hit
GTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTACCCGGCTGAGAG	16438	0.110229480907527	No Hit
GTCTGCCGTAGACGGTGAGAGCCCGGTACGCGAAAACCCGGCACCTGCCT	15074	0.10108280783550688	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0117316140806727E-5	6.7057720469355765E-6	3.352886023467788E-5	0.0	6.7057720469355765E-6
2	2.0117316140806727E-5	6.7057720469355765E-6	1.7435007322032497E-4	0.0	6.7057720469355765E-6
3	4.6940404328549037E-5	6.7057720469355765E-6	1.9446738936113172E-4	0.0	6.7057720469355765E-6
4	7.376349251629133E-5	6.7057720469355765E-6	2.1458470550193845E-4	0.0	1.3411544093871153E-5
5	8.717503661016248E-5	6.7057720469355765E-6	3.2187705825290763E-4	0.0	1.3411544093871153E-5
6	1.408212129856471E-4	6.7057720469355765E-6	3.285828302998432E-4	0.0	1.3411544093871153E-5
7	1.408212129856471E-4	6.7057720469355765E-6	3.4199437439371436E-4	0.0	1.3411544093871153E-5
8	1.7435007322032497E-4	6.7057720469355765E-6	3.6881746258145666E-4	0.0	1.3411544093871153E-5
9	1.8105584526726054E-4	6.7057720469355765E-6	3.8893477872226344E-4	0.0	2.0117316140806727E-5
10-11	2.2129047754887402E-4	6.7057720469355765E-6	4.258165249804091E-4	0.0	2.0117316140806727E-5
12-13	2.984068560886331E-4	6.7057720469355765E-6	4.727569293089581E-4	0.0	2.3470202164274515E-5
14-15	4.124049808865379E-4	6.7057720469355765E-6	4.995800174967004E-4	0.0	2.6823088187742306E-5
16-17	5.632848519425884E-4	6.7057720469355765E-6	5.029329035201682E-4	0.0	2.6823088187742306E-5
18-19	7.443406972098489E-4	6.7057720469355765E-6	5.364617637548461E-4	0.0	4.358751830508124E-5
20-21	0.0010092186930638041	6.7057720469355765E-6	5.632848519425884E-4	0.0	4.6940404328549037E-5
22-23	0.0013378015233636473	6.7057720469355765E-6	6.16931028318073E-4	0.0	4.6940404328549037E-5
24-25	0.0017770295924379278	6.7057720469355765E-6	7.543993552802523E-4	0.0	6.705772046935576E-5
26-27	0.002310138470169306	6.7057720469355765E-6	9.18690770430174E-4	0.0	8.046926456322691E-5
28-29	0.0030745964835199617	6.7057720469355765E-6	9.689840607821908E-4	0.0	8.717503661016248E-5
30-31	0.004006698798044007	6.7057720469355765E-6	9.857484908995296E-4	0.0	9.723369468056586E-5
32-33	0.005404852269830075	6.7057720469355765E-6	9.857484908995296E-4	0.0	1.139981247979048E-4
34-35	0.007208704950455744	6.7057720469355765E-6	9.991600349934008E-4	0.0	1.2070389684484038E-4
36-37	0.009099732667691577	6.7057720469355765E-6	0.0010360417812515464	0.0	1.2070389684484038E-4
38-39	0.01138304804967314	6.7057720469355765E-6	0.00107627641353316	0.0	1.2405678286830815E-4
40-41	0.014444232989099231	6.7057720469355765E-6	0.0011299225899086445	0.0	1.5087987105605045E-4
42-43	0.018859983882006305	6.7057720469355765E-6	0.0011768629942371935	0.0	1.5423275707951824E-4
44-45	0.023245558800702174	6.7057720469355765E-6	0.001247273600730017	0.0	1.6093852912645382E-4
46-47	0.028425767706959905	6.7057720469355765E-6	0.001294214005058566	0.0	1.676443011733894E-4
48-49	0.03434696442440402	6.7057720469355765E-6	0.0013545659534809863	0.0	1.676443011733894E-4
50-51	0.040797917133556044	6.7057720469355765E-6	0.0013981534717860675	0.0	1.676443011733894E-4
52-53	0.049304188975093824	6.7057720469355765E-6	0.0014283294459972776	0.0	1.676443011733894E-4
54-55	0.06001330693404994	6.7057720469355765E-6	0.00145515253418502	0.0	1.7770295924379276E-4
56-57	0.07407195803045037	6.7057720469355765E-6	0.0014652111922554233	0.0	1.8440873129072833E-4
58-59	0.09156396441488182	1.0058658070403365E-5	0.0014719169643023589	0.0	1.8776161731419615E-4
60-61	0.11205680379031695	1.3411544093871153E-5	0.0015087987105605045	0.0	1.9446738936113172E-4
62-63	0.1395907038150344	1.3411544093871153E-5	0.0015926208611471994	0.0	1.978202753845995E-4
64-65	0.1710608920313031	1.3411544093871153E-5	0.0016395612654757485	0.0	2.011731614080673E-4
66-67	0.20407005493234345	1.3411544093871153E-5	0.0016898545558277653	0.0	2.011731614080673E-4
68-69	0.24177661115226218	2.0117316140806727E-5	0.001709971871968572	0.0	2.011731614080673E-4
70-71	0.290091698750433	2.0117316140806727E-5	0.0017233834160624432	0.0	2.011731614080673E-4
72-73	0.35206644400821163	2.0117316140806727E-5	0.0017300891881093787	0.0	2.011731614080673E-4
74-75	0.431865131366745	2.0117316140806727E-5	0.001740147846179782	0.0	2.011731614080673E-4
76-77	0.5245254895113007	2.0117316140806727E-5	0.001756912276297121	0.0	2.011731614080673E-4
78-79	0.6336216949428957	2.0117316140806727E-5	0.001820617110743009	0.0	2.0787893345500287E-4
80-81	0.7582551742072403	2.0117316140806727E-5	0.001867557515071558	0.0	2.0787893345500287E-4
82-83	0.9087259931684277	2.0117316140806727E-5	0.0018809690591654292	0.0	2.1458470550193845E-4
84-85	1.0918773922003555	2.0117316140806727E-5	0.0018910277172358326	0.0	2.3470202164274518E-4
86-87	1.3019859948609644	2.0117316140806727E-5	0.001931262349517446	0.0	2.5817222380701966E-4
88-89	1.5640542722272537	2.0117316140806727E-5	0.002028496044198012	0.0	2.6823088187742303E-4
90	1.7862366174583701	2.0117316140806727E-5	0.0020452604743153506	0.0	2.6823088187742303E-4
>>END_MODULE