FastQCFastQC Report
Thu 25 Jan 2024
SRR25787973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR25787973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14912526
Sequences flagged as poor quality0
Sequence length101
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCA2929521.964469332693871No Hit
CCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTA2310441.5493283968121834No Hit
GCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT1949861.3075316683437803No Hit
ATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGA1793651.2027808031985996No Hit
CGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATT1644031.1024490418323496No Hit
CGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACC1139870.7643708383140455No Hit
CTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGGGCTCTC857930.5753083012227439No Hit
GTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGC799890.5363880002623298No Hit
CCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACT745840.500143302348643No Hit
GGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGC696350.4669564364883589No Hit
CGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGGGCTCTCAC686270.4601970182650478No Hit
GGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCT632020.42381820491042227No Hit
CGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACA627260.42062625741608095No Hit
ATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGC601610.4034259521156912No Hit
CCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTAC575900.3861854121830198No Hit
CCGGGCTCTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACC565950.37951316899631893No Hit
CCACGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTT564420.3784871858731378No Hit
CGGCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTG560270.37570429047365955No Hit
CACGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTT512550.3437043462656829No Hit
CCGCTATCGGTCATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGG495040.3319625394114988No Hit
CTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCG478360.32077731163721024No Hit
GGCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTGA476430.31948309763215166No Hit
GAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTC420390.2819039510811247No Hit
CGGGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCG390540.261887221521022No Hit
GGCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCG386660.259285381966811No Hit
CGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTC381420.25577155741421675No Hit
GAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCAC365800.24529714147690337No Hit
GTCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT293140.19657300178386947No Hit
CTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGC283750.19027628183179698No Hit
GCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCG273990.18373144831398786No Hit
CGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGAT272540.1827591113671822No Hit
ACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTAC268320.17992927556337537No Hit
GTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACACTTTCTG251650.16875075356113378No Hit
CTCGGTTACCAAGCGTGCACTCCTGGGGTTGCGTGGGTGGCCTCGGCGAT248310.1665110256974573No Hit
CTCTGCTCGGATCTCCCGGTCACGCGAACTAGCCGAGATGACGATCCCAG247300.1658337427207168No Hit
CCCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTAT243650.1633861359235853No Hit
CACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACACTT242280.16246744515315514No Hit
CTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAA240500.16127381772880062No Hit
CCGCTAACCACGACACTTTCTGACTGCCTCTCAGCCGGGTAGCGCTGAGA235790.15811539909469396No Hit
CCCCGCTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGT234190.15704247556718426No Hit
CTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGACAC232020.15558732303299924No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA224190.15033670352024867No Hit
GGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGATA221540.14855967392781075No Hit
CGGGCTCTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCA213060.14287317923200937No Hit
CCGGCGGATTCACAGCGGATTCCACGGGCCCGCTGCTACTCGGGAATTGA203710.1366032823681246No Hit
CATCGAGGAGTATTTAGGCTTACCGGGTGGTCCCGGCGGATTCACAGCGG200850.13468543156270105No Hit
CTGCTACTCGGGAATTGATACTAGGCAGGTGCCGGGTTTTCGCGTACCGG198540.13313639821985893No Hit
GTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCCGGTCACGCGAACTAG196230.13158736487701683No Hit
CTCTCACCGTCTACGGCAGACCATCCCAGGCCACTTCCGCTAACCACGAC188470.1263836857685948No Hit
GCCACTTCCGCTAACCACGACACTTTCTGACTGCCTCTCAGCCGGGTAGC185300.12425795602971623No Hit
CCTCAAGGCTTCTCCGTGCGCAGTTCGCTATGCCTCTGCTCGGATCTCCC180550.12107271430742182No Hit
GCCGGTTAGCACCAGTTCCCTACACCCATTACTGAGCTTCCAGGTCTGAC174390.11694195872650952No Hit
CCCGTGTTCTGCGGGGGGCCTTATCCCACCAAGTGGGTGAGAAACCTGAT169650.11376342277626204No Hit
CCGGGTCCTACGGCATTCCCTCAAGGCTTCTCCGTGCGCAGTTCGCTATG165610.11105429086930008No Hit
CCCAGTCGCTGTGGTTGTCCCGAGGAGTCCCGCGACCGGACTCATCGGTG161080.10801657613203824No Hit
GCTTACCGGGTGGTCCCGGCGGATTCACAGCGGATTCCACGGGCCCGCTG159580.10701071032499791No Hit
CTCCTGGGGTTGCGTGGGTGGCCTCGGCGATCTTGCGACGCTTCTAGCTG154160.10337618187555885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)