[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 848600 sequences (84860000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 354, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 153, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (149.83, 53.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 848600 reads in 618.688 CPU sec, 40.494 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 16 ../influenzaA/2-alignment/host/indices/bwa/Homo_sapiens_GRCh38 ../influenzaA/1-quality/fastq/DRR051450_1.fastq ../influenzaA/1-quality/fastq/DRR051450_2.fastq [main] Real time: 48.503 sec; CPU: 624.266 sec