[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 830136 sequences (83013600 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 1365, 2, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (102, 154, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (158.11, 87.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 830136 reads in 356.173 CPU sec, 22.485 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 16 ../influenzaA/2-alignment/host/indices/bwa/Homo_sapiens_GRCh38 ../influenzaA/1-quality/fastq/DRR051428_1.fastq ../influenzaA/1-quality/fastq/DRR051428_2.fastq [main] Real time: 30.254 sec; CPU: 361.191 sec