[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 418096 sequences (41809600 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 701, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 158, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (158.25, 55.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 418096 reads in 295.361 CPU sec, 18.700 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 16 ../influenzaA/2-alignment/host/indices/bwa/Homo_sapiens_GRCh38 ../influenzaA/1-quality/fastq/DRR051423_1.fastq ../influenzaA/1-quality/fastq/DRR051423_2.fastq [main] Real time: 22.687 sec; CPU: 297.973 sec