[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 261972 sequences (26197200 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11199, 4, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 159, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (165.77, 49.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 261972 reads in 15.090 CPU sec, 0.992 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 16 -k 50 ../influenzaA/2-alignment/pathogen/indices/bwa/influenzaA ../influenzaA/2-alignment/host/fastq/DRR051431_1.fastq ../influenzaA/2-alignment/host/fastq/DRR051431_2.fastq [main] Real time: 1.415 sec; CPU: 15.327 sec