[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 654772 sequences (65477200 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 190, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 120, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (126.32, 42.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 324) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 654772 reads in 131.037 CPU sec, 8.320 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 16 -k 50 -w 50 ../influenzaA/2-alignment/host/indices/bwa/Homo_sapiens_GRCh38 ../influenzaA/1-quality/fastq/DRR051431_1.fastq ../influenzaA/1-quality/fastq/DRR051431_2.fastq [main] Real time: 17.154 sec; CPU: 135.696 sec