[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 418096 sequences (41809600 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 423, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 165, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (28, 313) [M::mem_pestat] mean and std.dev: (170.33, 46.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 418096 reads in 71.309 CPU sec, 4.506 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 16 -k 50 -w 50 ../influenzaA/2-alignment/host/indices/bwa/Homo_sapiens_GRCh38 ../influenzaA/1-quality/fastq/DRR051423_1.fastq ../influenzaA/1-quality/fastq/DRR051423_2.fastq [main] Real time: 13.346 sec; CPU: 75.781 sec