FastQCFastQC Report
Fri 12 Jan 2024
DRR051450_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR051450_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424300
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG246225.802969596983266Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG189034.455102521800613Illumina Single End PCR Primer 1 (95% over 22bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG157413.7098750883808624Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG87372.0591562573650717Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGCGCCG61041.4386047607824652Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGGGGGCGCCG58751.384633514023097No Hit
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGGGGGCGGCG58121.369785529106764Illumina Single End PCR Primer 1 (95% over 22bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGGCG57571.3568230025925052Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG50951.2008013198208816Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG41200.9710110770681122Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG35790.8435069526278576Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGCGCCG29810.7025689370728257Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG26710.6295074239924582Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGGCG25300.5962762196559038Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG25060.5906198444496819Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGGGGGCGCCG22540.5312279047843507Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGGAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG20920.4930473721423521No Hit
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGGGGGCGGCG19930.46971482441668627Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGGGGGCGGCG18320.431769974074947No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG17690.41692198915861417Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGTAGAACTCGGGGGGCGCCG17430.4107942493518737No Hit
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGAACTCGGGGGGCGCCG17170.4046665095451331Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG16940.3992458166391704Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGGAGAGCGGCGTGGAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG16750.3947678529342446No Hit
GATCGGGAGAGCGGCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG16500.38887579542776335Illumina Single End PCR Primer 1 (96% over 27bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG15840.3733207636106528Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGTCGCCG14670.34574593448032054Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGCGGCG14650.34527456987980204Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATATCGGGGGGCGCCG14630.34480320527928354Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG11930.2811689842092859No Hit
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGTAGATATCGGGGGGCGCCG11770.27739806740513784No Hit
GATCGGGAGAGCGTCGTGTAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG11760.2771623851048786Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG10850.2557152957812868Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGGAGAACTCGGGGGGCGCCG10240.24133867546547255No Hit
GATCGGAAGAGCGTCGTGGAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG10150.23921753476313928Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGTCGCCG9930.23403252415743575Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGAACTCGGGGGGCGCCG8820.20787178882865895Illumina Single End PCR Primer 1 (97% over 35bp)
GATTGGGAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG8800.20740042422814045No Hit
GATTGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG8350.1967947207164742Illumina Single End PCR Primer 1 (97% over 34bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG8060.18995993400895592Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGGAGAACTCGGGGGGCGCCG7980.18807447560688192Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG7930.18689606410558565Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGAACTCGGGGGGCGCCG7730.18218241810040064Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGGCGTGGAGGGGAAGAGTGGAGATCTCGGGGGGCGCCG7540.17770445439547491No Hit
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGTAGAACTCGGGGGGCGGCG7400.1744049021918454No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATATCGGGGGGCGCCG7240.17063398538769736Illumina Single End PCR Primer 1 (97% over 36bp)
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG6850.16144237567758662Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGCGCCG6760.15932123497525336Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGGCGTGGAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG6730.1586141880744756Illumina Single End PCR Primer 1 (95% over 22bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG6600.15555031817110535Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGAGTAGATCTCGGGGGGCGCCG6580.15507895357058685Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGGCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGCGCCG6540.15413622436954985No Hit
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGTGGGCGCCG6480.15272213056799433No Hit
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGGGGGGGCCG6460.15225076596747583No Hit
GATCGGGAGAGCGTCGTGGAGGGGAAGAGGGTAGATCTCGGGGGGCGGCG6400.15083667216592034No Hit
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGGGGTCGCCG6210.14635870846099458Illumina Single End PCR Primer 1 (95% over 22bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGGCG5990.14117369785529105Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGGCGTGGAGGGGAAGAGTGTAGATCTCGGGGGGCGGCG5910.13928823945321706No Hit
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGGGGGCGGCG5710.13457459344803205Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGCGCCG5480.1291539005420693Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGCGGCG5470.12891821824181005Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG5370.12656139523921753Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGCCC5360.12632571293895828Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATATCGGGGGGCGCCG5230.12326184303558803Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGGAGATATCGGGGGGCGCCG5160.12161206693377329No Hit
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGGGCCG5120.12066933773273628Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGAACTCGGGGGGCGGCG5050.11901956163092153Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGGGGGCGCCC5040.11878387933066227Illumina Single End PCR Primer 1 (95% over 22bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGGCGCCG4950.116662738628329Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGGAGAGCGTCGTGGAGGGGAAGAGAGTAGATCTCGGGGGGCGCCG4920.11595569172755127No Hit
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGGGGGGGCCG4870.11477728022625501Illumina Single End PCR Primer 1 (95% over 22bp)
GATCGGGAGAGCGGCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGGCG4820.11359886872495877Illumina Single End PCR Primer 1 (96% over 27bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATTTCGGGGGGCGCCG4790.112891821824181Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGGGCCG4660.10982795192081074Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGTAGATTTCGGGGGGCGCCG4540.10699976431769974No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG4490.1058213528164035Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGGAGAGCGTCGTGGAGGGGAAGAGGGGAGATCTCGGGGGGCGGCG4470.105349988215885No Hit
GAACGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG4450.10487862361536648Illumina Single End PCR Primer 1 (97% over 34bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGAGTAGATCTCGGGGGGCGCCG4340.10228611831251473Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGGGGTCGCCG4310.10157907141173697Illumina Single End PCR Primer 1 (96% over 26bp)
GATTGGGAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGGGGGCGCCG4280.10087202451095922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)