FastQCFastQC Report
Fri 12 Jan 2024
DRR051450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR051450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424300
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC30312171.44025453688428TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGACATCTCGTATGC21320.5024746641527221TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGAGATCTCGTATGC20180.4756068819231676TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGAAATCTCGTATGC18550.4371906669809097TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATATCGTATGC17670.4164506245580957TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGCTATCTCGTATGC17560.4138581192552439TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGCC8760.20645769502710345TruSeq Adapter, Index 7 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATCTCGTATGCCG8030.18925288710817817TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGGTATCTCGTATGC7200.1696912561866604TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGATATCTCGTATGC6770.1595569172755126TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGATATCTCGTATGC6470.15248644826773508TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTATATCTCGTATGC6010.14164506245580957TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAATATCTCGTATGC5860.1381098279519208TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACG5840.1376384633514023Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGCATGC5200.12255479613481027TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGATATCTCGTATGC5060.11925524393118078TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCGATATCTCGTATGC4660.10982795192081074TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGACTCCAGTCACCACGATATCTCGTATGCC4570.1077068112184775RNA PCR Primer, Index 31 (100% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCATATCTCGTATGC4530.10676408201744049TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTTATCTCGTATGC4390.10346452981381098TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)