##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	DRR051450_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	424300
Sequences flagged as poor quality	0
Sequence length	151
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.730056563752065	34.0	33.0	34.0	32.0	35.0
2	33.91243931180768	34.0	33.0	35.0	33.0	35.0
3	33.91810511430592	34.0	33.0	35.0	33.0	35.0
4	34.032387461701624	34.0	33.0	35.0	33.0	36.0
5	33.96333490454867	34.0	33.0	35.0	33.0	35.0
6	34.313469243459814	34.0	34.0	35.0	33.0	36.0
7	34.128166391703985	34.0	34.0	35.0	33.0	36.0
8	34.029625265142585	34.0	33.0	35.0	33.0	36.0
9	34.07823709639406	34.0	33.0	35.0	33.0	36.0
10-14	36.970449681828896	37.4	37.4	37.4	37.4	37.4
15-19	37.682758425642234	38.0	38.0	38.0	38.0	38.0
20-24	38.04000848456282	38.4	38.4	38.4	38.0	38.4
25-29	38.517193966533114	39.0	39.0	39.0	38.0	39.0
30-34	38.17409992929531	39.0	38.8	39.0	37.0	39.0
35-39	35.423350930945084	38.6	36.8	38.6	20.4	38.6
40-44	37.092925760075424	38.6	37.8	38.6	34.8	38.6
45-49	36.854718831015795	38.6	38.2	38.6	33.6	38.6
50-54	36.29111854819703	38.4	38.0	38.4	32.2	38.4
55-59	37.12217063398539	38.8	38.2	38.8	34.2	38.8
60-64	36.685802969596985	39.0	38.0	39.0	29.8	39.0
65-69	37.73038887579543	38.8	38.4	38.8	36.4	38.8
70-74	36.40484279990572	37.6	36.4	38.0	34.8	38.0
75-79	26.154950271034647	25.8	20.0	33.0	14.0	37.4
80-84	22.04716474192788	18.0	14.4	30.0	14.0	38.0
85-89	22.12398114541598	17.0	16.6	30.0	14.0	38.0
90-94	21.96079283525807	17.0	16.0	30.0	14.0	38.0
95-99	21.7898138109828	17.0	16.0	30.0	14.0	38.0
100-104	21.816676408201744	17.0	16.0	30.0	14.0	38.0
105-109	21.784676879566344	17.0	15.4	29.8	14.0	38.0
110-114	21.27156257365072	16.2	15.0	28.6	14.0	38.0
115-119	20.91475371199623	16.0	15.0	27.6	13.0	38.0
120-124	20.709584727786943	16.0	15.0	27.0	13.0	38.0
125-129	20.529494697148245	16.0	15.0	27.0	13.0	38.0
130-134	20.35853499882159	16.0	15.0	27.0	13.0	38.0
135-139	20.185936837143533	15.8	15.0	27.0	13.0	38.0
140-144	20.039614895121375	15.0	15.0	26.6	13.0	38.0
145-149	19.903067640820176	15.0	15.0	26.0	13.0	38.0
150-151	19.622613716709875	15.0	15.0	25.5	13.0	37.5
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.23670015375594033
1101	2	-0.02220211455937715
1101	3	0.1680578320094952
1101	4	0.10028859668270229
1101	5	0.1265619477975477
1101	6	0.15343501255591718
1101	7	0.21317618558486373
1101	8	0.18447658562759273
1101	9	0.12864539387727802
1101	10-14	0.09177650297520046
1101	15-19	-0.03273480586329924
1101	20-24	0.03625410522393224
1101	25-29	0.02171465936073247
1101	30-34	-0.011892840616269496
1101	35-39	0.07328335865962288
1101	40-44	0.1024814650192667
1101	45-49	0.07694201782344123
1101	50-54	0.04662486404909316
1101	55-59	0.009226264937751694
1101	60-64	0.09796028380430499
1101	65-69	0.14802244539112763
1101	70-74	0.33278007537686705
1101	75-79	0.5895242265917844
1101	80-84	-0.07422383970346402
1101	85-89	-0.020694873179106565
1101	90-94	-0.11046223748915907
1101	95-99	0.03954994154933544
1101	100-104	0.10841721359005518
1101	105-109	0.21658513964038661
1101	110-114	-0.03628178685695005
1101	115-119	-0.13795581495680054
1101	120-124	0.09224253909711067
1101	125-129	-0.00564117424712407
1101	130-134	0.01783065475743939
1101	135-139	-0.08719847417205173
1101	140-144	0.039886772418007865
1101	145-149	0.03453657523774112
1101	150-151	0.08704830945029585
1102	1	0.038875282699237346
1102	2	-0.0016504531430143743
1102	3	0.17523426137104536
1102	4	0.06528937896703013
1102	5	0.09397957691792413
1102	6	0.05434337732548755
1102	7	0.10950338355220168
1102	8	-0.012173682041741074
1102	9	0.15400604525021322
1102	10-14	-0.02888531871338529
1102	15-19	-0.01935929932275826
1102	20-24	0.042430674573211036
1102	25-29	0.04365091828495338
1102	30-34	0.0572831440784114
1102	35-39	0.17864013063426398
1102	40-44	0.19518197455627728
1102	45-49	0.11021947806189303
1102	50-54	0.06979722370225971
1102	55-59	0.053935598275970165
1102	60-64	0.12039949207767364
1102	65-69	0.04203792455627564
1102	70-74	-0.09659767462702007
1102	75-79	0.30577780748684447
1102	80-84	-0.5293176086029554
1102	85-89	-0.3987462623971254
1102	90-94	-0.43933520823904715
1102	95-99	-0.36284073764729996
1102	100-104	-0.3580043983182293
1102	105-109	-0.189762342822263
1102	110-114	-0.29048384312103437
1102	115-119	-0.39083439253515806
1102	120-124	-0.39498646343090016
1102	125-129	-0.39740413203213265
1102	130-134	-0.4136868029923413
1102	135-139	-0.28554963840326764
1102	140-144	-0.38288428910064454
1102	145-149	-0.48440358046886445
1102	150-151	-0.0768059459476973
1103	1	0.15553164296260036
1103	2	0.1601788728182072
1103	3	0.18575952418433417
1103	4	0.2285282571453422
1103	5	0.1732846222054718
1103	6	0.21063088960223553
1103	7	0.2773419489008617
1103	8	0.24533241086139412
1103	9	0.2002748241232979
1103	10-14	0.07573455688227426
1103	15-19	0.07440411415801407
1103	20-24	0.09332642227245458
1103	25-29	0.09671355696669792
1103	30-34	0.10632155664979592
1103	35-39	0.19918612750596765
1103	40-44	0.22902996117007746
1103	45-49	0.1231786283292493
1103	50-54	0.16885491531042618
1103	55-59	0.09937683358934635
1103	60-64	0.2191810933289915
1103	65-69	0.19628204081251255
1103	70-74	0.31981169571236734
1103	75-79	0.6420996946499145
1103	80-84	0.03236967087032738
1103	85-89	-0.07879664252152452
1103	90-94	0.01981942418611382
1103	95-99	-0.11629568653966871
1103	100-104	0.11032159928098295
1103	105-109	0.05722066980004925
1103	110-114	-0.08936729588739567
1103	115-119	0.02043165817234538
1103	120-124	-0.03109082485827841
1103	125-129	-0.08340864259922753
1103	130-134	-0.06823756505992762
1103	135-139	-0.1261511079751294
1103	140-144	0.017276854546363296
1103	145-149	-0.026846561808206104
1103	150-151	0.21637773234701996
1104	1	0.1645960352237097
1104	2	0.19930160210487458
1104	3	0.21903008983194638
1104	4	0.19177593104875257
1104	5	0.16799736773788965
1104	6	0.15847832720377397
1104	7	0.2776299418118029
1104	8	0.14998460403817404
1104	9	0.10989304890545526
1104	10-14	0.05340329119034948
1104	15-19	0.06790581257774875
1104	20-24	0.06396591422342368
1104	25-29	-0.0138491368746827
1104	30-34	0.13969328903791478
1104	35-39	0.12469860719878056
1104	40-44	0.20803380107555824
1104	45-49	0.06608809517314995
1104	50-54	0.13966498767786817
1104	55-59	0.06791176196033888
1104	60-64	0.12817828724422498
1104	65-69	0.20594826441315917
1104	70-74	0.16764288755994272
1104	75-79	0.5211057498854821
1104	80-84	-0.2687372557284
1104	85-89	-0.18003501203858718
1104	90-94	-0.11318825562484847
1104	95-99	-0.04818831687491709
1104	100-104	-0.06584883344682879
1104	105-109	-0.14789452736432906
1104	110-114	-0.17655456512384404
1104	115-119	-0.12730722825506646
1104	120-124	-0.13282321298410693
1104	125-129	-0.19388715389741407
1104	130-134	-0.20400702304250373
1104	135-139	-0.20959151559586076
1104	140-144	-0.13957294607392612
1104	145-149	-0.15939714517846326
1104	150-151	-0.1662799253106364
1105	1	0.2238018037378211
1105	2	0.23574961574209397
1105	3	0.19784138997990652
1105	4	0.1635301733086365
1105	5	0.19074497587447325
1105	6	0.25618082846749957
1105	7	0.17106149559137407
1105	8	0.3471044056786283
1105	9	0.21628254352399523
1105	10-14	0.06071484777410063
1105	15-19	0.08901896533600251
1105	20-24	0.07108569741526338
1105	25-29	0.04890227167735617
1105	30-34	0.05495186487834758
1105	35-39	0.3450187460799796
1105	40-44	0.20302297173635253
1105	45-49	0.2606693668733371
1105	50-54	0.3705655016563938
1105	55-59	0.2518272414583791
1105	60-64	0.36860834299594814
1105	65-69	0.20483406008364113
1105	70-74	0.16305001167857114
1105	75-79	0.3490348370847194
1105	80-84	0.4000190859843009
1105	85-89	0.2909034323000874
1105	90-94	0.13837627948841202
1105	95-99	0.30902887502392673
1105	100-104	0.47024367530704225
1105	105-109	0.3630481786052755
1105	110-114	0.20259093175280896
1105	115-119	0.17049452657754927
1105	120-124	0.3610148561096551
1105	125-129	0.18406859155592414
1105	130-134	0.3002367127289993
1105	135-139	0.2157275532654488
1105	140-144	0.3117581217690244
1105	145-149	0.41128873756949247
1105	150-151	0.3022239009328942
1106	1	-0.008657546226594093
1106	2	0.1176113052506409
1106	3	0.19319195661676503
1106	4	0.1501498787669604
1106	5	0.15166300058385218
1106	6	0.15995521392655832
1106	7	0.3036933002522133
1106	8	0.21154862707760458
1106	9	0.22730185115032953
1106	10-14	0.03478861093632446
1106	15-19	0.06345816821207251
1106	20-24	0.056839935785980344
1106	25-29	0.10914598939913134
1106	30-34	0.08861885394708935
1106	35-39	0.22540234372218748
1106	40-44	0.26754347468359185
1106	45-49	0.18115160130223273
1106	50-54	0.10007113152663294
1106	55-59	0.12234980656231897
1106	60-64	0.15972163386953042
1106	65-69	0.15344420297467565
1106	70-74	0.20616304706371835
1106	75-79	0.790478073028293
1106	80-84	-0.28100870750804674
1106	85-89	-0.10258042630530895
1106	90-94	-0.10058598121929663
1106	95-99	-0.21913352437750078
1106	100-104	-0.002651373691985981
1106	105-109	-0.08359014101075957
1106	110-114	-0.1180159445360438
1106	115-119	-0.0824061796654938
1106	120-124	-0.1394692032366578
1106	125-129	-0.09813837232896105
1106	130-134	0.07365432683196005
1106	135-139	-0.06844840527242724
1106	140-144	-0.14542584815633575
1106	145-149	-0.034414129375775815
1106	150-151	-0.05287902440973724
1107	1	0.139634446030918
1107	2	0.14543588048901057
1107	3	0.03125740933739252
1107	4	0.13347484829883172
1107	5	0.1169490425940296
1107	6	0.18467920847814412
1107	7	0.2738685816343889
1107	8	0.2839803426127716
1107	9	0.22799903374406227
1107	10-14	0.0692111501864403
1107	15-19	0.09061674588788549
1107	20-24	0.06343359841412877
1107	25-29	0.18634327952745622
1107	30-34	0.15088496621320502
1107	35-39	0.2762332276565189
1107	40-44	0.21745314562774354
1107	45-49	0.21155127152948694
1107	50-54	0.19944814780020437
1107	55-59	0.31649454812085054
1107	60-64	0.17239673602739458
1107	65-69	0.1404697962071637
1107	70-74	0.34382130213576545
1107	75-79	0.4801543939115156
1107	80-84	-0.291843892825991
1107	85-89	-0.21837044709532805
1107	90-94	-0.1519871569308151
1107	95-99	-0.15160144322473101
1107	100-104	-0.1411050262835758
1107	105-109	0.03364980450504618
1107	110-114	-0.1223090838291796
1107	115-119	0.04364586707621143
1107	120-124	-0.09684786837394199
1107	125-129	-0.17402804183931764
1107	130-134	-0.11658547530439023
1107	135-139	-0.1069042085558145
1107	140-144	-0.1253297839223393
1107	145-149	-0.1149138067720088
1107	150-151	-0.0857648943645728
1108	1	0.18746787607070559
1108	2	0.1670376847551438
1108	3	0.11155485076091054
1108	4	0.12587130331651508
1108	5	0.1805059059892571
1108	6	0.18636639072835948
1108	7	0.19565205588284584
1108	8	0.1252556935190512
1108	9	0.2215093398890673
1108	10-14	0.04430578435974297
1108	15-19	0.03514454208593776
1108	20-24	0.05845874209228441
1108	25-29	0.06306983511984754
1108	30-34	0.09864137646960813
1108	35-39	0.19396090228074314
1108	40-44	0.23247027648539387
1108	45-49	0.36961866211304084
1108	50-54	0.33895204368879206
1108	55-59	0.24770172097672827
1108	60-64	0.3434638917524211
1108	65-69	0.1215882063530529
1108	70-74	0.13857014165831316
1108	75-79	0.7444258489291933
1108	80-84	0.07922637132077881
1108	85-89	0.0291756266226173
1108	90-94	0.11515233522214885
1108	95-99	0.020890405802678202
1108	100-104	0.23408286054224448
1108	105-109	0.12070391867392516
1108	110-114	0.0188667975810759
1108	115-119	0.1751283079471193
1108	120-124	0.12312581365523556
1108	125-129	0.1674324328512249
1108	130-134	0.11404284034547629
1108	135-139	0.13885452265550313
1108	140-144	0.04976277796979289
1108	145-149	0.16233910823683573
1108	150-151	0.12310809552269575
1109	1	0.11104234847330474
1109	2	0.10252706516639876
1109	3	0.10497679340159749
1109	4	0.12870375732084227
1109	5	0.13791256183341005
1109	6	0.17398645295779858
1109	7	-0.4194639479050366
1109	8	-0.12579781026882841
1109	9	-0.05657845772998371
1109	10-14	0.005831081978790564
1109	15-19	0.028349358103255895
1109	20-24	-0.04181046786443687
1109	25-29	0.041706536959672746
1109	30-34	0.015340510668750085
1109	35-39	0.3513202096400505
1109	40-44	0.1528084799486038
1109	45-49	-0.013686938536309867
1109	50-54	0.050804722260224366
1109	55-59	0.04453478374728803
1109	60-64	0.013145642293544313
1109	65-69	0.039484919015393416
1109	70-74	0.21303036893103666
1109	75-79	1.0300586276088488
1109	80-84	0.18076384426450431
1109	85-89	0.2807804020555196
1109	90-94	0.34382082818751414
1109	95-99	0.2614579112139346
1109	100-104	0.30595515491453895
1109	105-109	0.22425471683409626
1109	110-114	0.4425860210659245
1109	115-119	0.25886444160512667
1109	120-124	0.17183827307081856
1109	125-129	0.25102449183390263
1109	130-134	0.3568431650208055
1109	135-139	0.31705528023126206
1109	140-144	0.27741023467974557
1109	145-149	0.22672662176497127
1109	150-151	0.20455340802269717
1110	1	0.09686365126768237
1110	2	0.10671309581336175
1110	3	0.07374997688259555
1110	4	0.07930109170018795
1110	5	0.10298107834644554
1110	6	-0.033126985147106325
1110	7	0.15044890352456264
1110	8	0.2290132683439552
1110	9	0.12898105826288742
1110	10-14	0.067658302689793
1110	15-19	-0.001421514490985487
1110	20-24	0.04726202235054444
1110	25-29	0.09108907825292079
1110	30-34	0.09740751785722779
1110	35-39	0.035731530426943436
1110	40-44	0.05437263476947862
1110	45-49	0.007369503380282083
1110	50-54	-0.027247993244884583
1110	55-59	0.011720447722197491
1110	60-64	0.04831802080143888
1110	65-69	0.01809310006611753
1110	70-74	0.19686072767275675
1110	75-79	1.214096198704862
1110	80-84	-0.2567848250569824
1110	85-89	-0.5651985697277873
1110	90-94	-0.5072611955012079
1110	95-99	-0.48678547376615455
1110	100-104	-0.5405532742793788
1110	105-109	-0.34999322170143543
1110	110-114	-0.33350661905823387
1110	115-119	-0.6114438187490947
1110	120-124	-0.4109555122640032
1110	125-129	-0.34056379512535884
1110	130-134	-0.45671594166371676
1110	135-139	-0.39820706084197965
1110	140-144	-0.4004181286837465
1110	145-149	-0.43881618080343543
1110	150-151	-0.3201018775977893
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2108	110-114	0.05027082135509886
2108	115-119	0.13073658536594124
2108	120-124	-0.03496962413469262
2108	125-129	0.11863226510768143
2108	130-134	-0.059091271649222676
2108	135-139	0.10376713034271035
2108	140-144	0.04516018519311871
2108	145-149	-0.012616626107099904
2108	150-151	-0.1295420194126926
2109	1	-0.1434515161650154
2109	2	-0.18547337825098253
2109	3	-0.21974969481799178
2109	4	-0.2135419811822814
2109	5	-0.256835073984476
2109	6	-0.2406122116736924
2109	7	-0.8219739461143121
2109	8	-0.41379697459760933
2109	9	-0.3115967486970561
2109	10-14	-0.09701668089522997
2109	15-19	-0.08586012250150077
2109	20-24	-0.14236371632313194
2109	25-29	-0.15355964137938827
2109	30-34	-0.12596740006792118
2109	35-39	-0.6166650552592046
2109	40-44	-0.2870587506540261
2109	45-49	-0.299130824919132
2109	50-54	-0.2403799696073321
2109	55-59	-0.29898211301319577
2109	60-64	-0.28864849265883663
2109	65-69	-0.15879462153598922
2109	70-74	-0.245686875741562
2109	75-79	-0.5387600061539715
2109	80-84	-0.002681082443722005
2109	85-89	0.15561561280668457
2109	90-94	0.03403887015867113
2109	95-99	0.16298471664750025
2109	100-104	0.13548086885916177
2109	105-109	0.08362463778641782
2109	110-114	0.005035731565531165
2109	115-119	0.06879255682543217
2109	120-124	0.12036425136042084
2109	125-129	0.05838564676564317
2109	130-134	0.2618658048891369
2109	135-139	0.07572596607857207
2109	140-144	0.04672566645742293
2109	145-149	0.1678811271414098
2109	150-151	0.08863088668323371
2110	1	-0.09585892966808274
2110	2	-0.16295455084291888
2110	3	-0.2132135914114741
2110	4	-0.1589637203202514
2110	5	-0.15724557817960516
2110	6	-0.23822099658474372
2110	7	-0.1517138879386195
2110	8	-0.2704488057113821
2110	9	-0.08569643737566679
2110	10-14	-0.06634381436496994
2110	15-19	-0.03865300628697099
2110	20-24	-0.05785199889277237
2110	25-29	-0.07855457396106402
2110	30-34	-0.16493602018825726
2110	35-39	-0.25753426042814453
2110	40-44	-0.2716221817380031
2110	45-49	-0.2717710971217855
2110	50-54	-0.20489071686529314
2110	55-59	-0.25758494349117456
2110	60-64	-0.3195634691754634
2110	65-69	-0.2551958883039447
2110	70-74	-0.4859310126952465
2110	75-79	-0.8004108237840839
2110	80-84	-0.07515261394746986
2110	85-89	-0.025149420101218567
2110	90-94	-0.13877074910063314
2110	95-99	-0.11207119819908229
2110	100-104	-0.13466164253603097
2110	105-109	-0.20405937013434539
2110	110-114	-0.14395511589737708
2110	115-119	0.07712815678177165
2110	120-124	-0.05265646701758797
2110	125-129	-0.15345071633523233
2110	130-134	-0.07788493010307462
2110	135-139	-0.07166383706305268
2110	140-144	-0.10781335439608597
2110	145-149	-0.13039430480186454
2110	150-151	-0.12202970543376779
2111	1	-0.23410944507077147
2111	2	-0.1639238472652167
2111	3	-0.2078139528296603
2111	4	-0.1333123616084606
2111	5	-0.2123045843644391
2111	6	-0.19614759306049478
2111	7	-0.021758598591965495
2111	8	-0.10429824873711624
2111	9	-0.0614245762416985
2111	10-14	-0.036621834879269954
2111	15-19	-0.05309132340173761
2111	20-24	-0.052389663588321866
2111	25-29	-0.06253950638604522
2111	30-34	4.125447440017638E-4
2111	35-39	-0.20251283834090827
2111	40-44	-0.1725947020783778
2111	45-49	-0.09972569078085769
2111	50-54	-0.17763808894785882
2111	55-59	0.008591659347580105
2111	60-64	-0.202277583997855
2111	65-69	-0.06782372089774924
2111	70-74	-0.14546422329345177
2111	75-79	-0.7072115941308219
2111	80-84	-0.08797837814331544
2111	85-89	-0.07054210180717746
2111	90-94	0.018599297306820972
2111	95-99	-0.06695441600868435
2111	100-104	-0.07183621769998183
2111	105-109	-0.17326772856616302
2111	110-114	-0.10566302891946222
2111	115-119	0.02045294956019461
2111	120-124	-0.175696282405859
2111	125-129	-0.10743836363859671
2111	130-134	-0.12915048762880232
2111	135-139	-0.06520429299294506
2111	140-144	0.08384068525810662
2111	145-149	-0.07940065931407503
2111	150-151	-0.15862987172006981
2112	1	-0.13764642256273163
2112	2	-0.1555637186478549
2112	3	-0.28369819720265355
2112	4	-0.1564634870103916
2112	5	-0.20265828211363868
2112	6	-0.1543469499736716
2112	7	-0.10600297158232053
2112	8	-0.2438480879654108
2112	9	-0.19968824183655443
2112	10-14	-0.07494980915579674
2112	15-19	-0.06505273819273327
2112	20-24	-0.10357937419924923
2112	25-29	-0.10626940992302991
2112	30-34	-0.1886549793717549
2112	35-39	-0.23068004091325633
2112	40-44	-0.26892910722307306
2112	45-49	-0.21766781296734194
2112	50-54	-0.14471204393176862
2112	55-59	-0.24517039853023448
2112	60-64	-0.20600748753482634
2112	65-69	-0.22718063408103006
2112	70-74	-0.3003195287596938
2112	75-79	-0.456664970336611
2112	80-84	0.6043688887377172
2112	85-89	0.35312349304812685
2112	90-94	0.41924238412353887
2112	95-99	0.4741388315797401
2112	100-104	0.314144489695515
2112	105-109	0.19534963477788736
2112	110-114	0.2302855241352013
2112	115-119	0.4590381585634127
2112	120-124	0.3553648108417278
2112	125-129	0.40767417655433036
2112	130-134	0.35252153809467046
2112	135-139	0.3430272182610743
2112	140-144	0.26236158557965084
2112	145-149	0.28458647894958844
2112	150-151	0.12754376172044601
2113	1	-0.1056710421672662
2113	2	-0.12176152011048202
2113	3	-0.11605575012769265
2113	4	-0.13896550449666734
2113	5	-0.16819167203414764
2113	6	-0.13094996303193085
2113	7	0.006679804311538362
2113	8	-0.1142184273630491
2113	9	-0.06238377075246149
2113	10-14	0.006577877445998581
2113	15-19	-0.007399210623205477
2113	20-24	-0.00786589488215128
2113	25-29	-0.04014846109818393
2113	30-34	-0.12381744451001708
2113	35-39	-0.3514443859723997
2113	40-44	-0.2320548102072877
2113	45-49	-0.20440299750984536
2113	50-54	-0.23443770761159044
2113	55-59	-0.3088252892096648
2113	60-64	-0.25344446460865555
2113	65-69	-0.11607078665735315
2113	70-74	-0.17454144806860228
2113	75-79	-0.8433912904834635
2113	80-84	0.09553576934852259
2113	85-89	0.15299596282764583
2113	90-94	0.023718485796486277
2113	95-99	0.05504969004628535
2113	100-104	0.023966909090145094
2113	105-109	-0.15736232672053063
2113	110-114	0.09673662988493348
2113	115-119	0.012304395669268331
2113	120-124	0.10108978878527353
2113	125-129	0.05089529723137076
2113	130-134	0.012518945677076232
2113	135-139	0.011490792703177988
2113	140-144	0.0332403733317328
2113	145-149	0.03683740824767057
2113	150-151	-0.0625467220163145
2114	1	-0.1791810875569979
2114	2	-0.17431578702587558
2114	3	-0.18402137488500614
2114	4	-0.16324000412736694
2114	5	-0.18330643517973755
2114	6	-0.1717139711416067
2114	7	-0.11575019025227107
2114	8	-0.01573081246358754
2114	9	-0.11770570405418113
2114	10-14	-0.09335933370463323
2114	15-19	-0.13411437892210643
2114	20-24	-0.19436117002123154
2114	25-29	-0.09972880025710396
2114	30-34	-0.12860872941772072
2114	35-39	-0.43452279106952574
2114	40-44	-0.28668606616601977
2114	45-49	-0.45519768953879947
2114	50-54	-0.413522741889345
2114	55-59	-0.36832895987883774
2114	60-64	-0.3420862922268313
2114	65-69	-0.19789787608023346
2114	70-74	-0.2734672630430879
2114	75-79	-0.6620496580064241
2114	80-84	0.27591838476847386
2114	85-89	0.12350717972511305
2114	90-94	0.059320093851965794
2114	95-99	0.12508519879293445
2114	100-104	0.06431898930124902
2114	105-109	0.03947658518175601
2114	110-114	0.2132201899466004
2114	115-119	-0.035570780098197474
2114	120-124	0.06522446756248002
2114	125-129	0.21517819073982736
2114	130-134	0.36046517198461103
2114	135-139	0.12333805717477375
2114	140-144	0.1573754156233278
2114	145-149	0.21569954211019393
2114	150-151	0.0651633190773353
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
15	1.0
16	2.0
17	21.0
18	44.0
19	73.0
20	65.0
21	184.0
22	534.0
23	4858.0
24	15228.0
25	15218.0
26	39674.0
27	190451.0
28	73383.0
29	12490.0
30	5041.0
31	2884.0
32	1951.0
33	1787.0
34	2186.0
35	3611.0
36	7957.0
37	25059.0
38	21598.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	89.09238746170163	2.9205750648126325	2.586848927645534	5.400188545840208
2	4.242752769267028	87.41055856705161	4.753947678529342	3.592740985152015
3	4.043836907848221	3.7513551732264907	87.49257600754183	4.712231911383455
4	4.872260193259486	4.347160028281876	3.2634928116898423	87.5170869667688
5	88.74310629271743	4.3761489512137635	3.4143294838557625	3.4664152722130566
6	87.84256422342682	4.8993636577893	3.6290360593919395	3.6290360593919395
7	3.538769738392647	87.5170869667688	4.928823945321707	4.015319349516852
8	3.922224840914448	87.42446382276691	4.088380862597219	4.564930473721423
9	87.69644119726608	3.6841857176526043	4.268442139995286	4.3509309450860245
10-14	20.82498232382748	37.70431298609475	3.716662738628329	37.754041951449445
15-19	20.91421164270563	20.616167805797787	20.692670280461936	37.77695027103464
20-24	20.92844685364129	37.537261371670986	20.62375677586613	20.91053499882159
25-29	20.92330897949564	20.709969361300967	20.575111949092623	37.79160971011077
30-34	4.272967240160264	21.037897713881687	20.45859061984445	54.2305444261136
35-39	21.03752062220127	36.94673580014141	20.373179354230498	21.64256422342682
40-44	4.504312986094744	20.74117369785529	37.2840914447325	37.47042187131747
45-49	37.189441432948385	20.744001885458403	37.04369549846807	5.022861183125147
50-54	21.235022389818525	3.930520857883573	20.629177468772095	54.20527928352581
55-59	20.718265378270093	3.8092387461701627	53.889276455338205	21.583219420221543
60-64	20.974876266792364	20.623568230025924	37.64746641527221	20.754089087909495
65-69	4.536130096629743	87.62488805090737	3.621069997643177	4.217911854819703
70-74	4.337638463351403	87.65896771152487	3.666650954513316	4.336742870610418
75-79	6.994155078953571	75.47956634456753	7.799387226019326	9.726891350459582
80-84	13.197030403016733	53.288569408437425	16.58171105349988	16.93268913504596
85-89	16.727221305679944	44.96431769974075	19.084609945793073	19.223851048786237
90-94	16.103228847513552	40.3884515672873	23.762667923638936	19.745651661560217
95-99	16.081121847749234	41.19589912797549	23.6649069054914	19.05807211878388
100-104	13.84082669848065	50.65927440927383	18.46227596619183	17.0376229260537
105-109	12.220468744255239	55.19387715661293	15.988655186099635	16.596998913032206
110-114	13.592370300433798	50.96000516615846	18.02541504851051	17.422209484897238
115-119	14.953120367363235	46.78102302289374	19.79041214764857	18.475444462094455
120-124	15.639529841206393	44.39749120431997	21.262118806728836	18.700860147744798
125-129	16.050260854153585	43.10433411957245	21.76152443657577	19.083880589698193
130-134	16.269406752138003	42.35200620695539	22.13891079434228	19.239676246564326
135-139	16.68018701923598	40.86937831046343	22.826330324917404	19.62410434538318
140-144	16.958096511232178	39.95811442313766	23.458327009173725	19.625462056456442
145-149	17.06696708445479	39.26961710873019	23.95835002757496	19.705065779240062
150-151	17.009527513124638	40.045840477495155	24.29993106252099	18.64470094685922
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	0.5
14	0.5
15	1.0
16	0.5
17	1.0
18	2.5
19	3.0
20	1.5
21	3.5
22	6.5
23	9.5
24	15.5
25	17.5
26	19.0
27	26.0
28	35.0
29	45.0
30	55.0
31	78.0
32	138.0
33	578.0
34	2637.5
35	8008.0
36	17732.5
37	30426.5
38	42115.0
39	49085.5
40	49842.0
41	45569.0
42	38082.0
43	29570.5
44	22366.0
45	16560.0
46	12200.5
47	9335.5
48	7145.5
49	5378.0
50	4045.0
51	3124.5
52	2412.0
53	2193.5
54	2077.0
55	1773.5
56	1645.0
57	1367.0
58	1178.5
59	1241.0
60	1190.0
61	1041.5
62	958.5
63	813.0
64	716.5
65	731.0
66	845.5
67	795.0
68	594.0
69	563.0
70	524.5
71	509.0
72	465.5
73	437.5
74	562.5
75	655.0
76	557.5
77	479.5
78	497.0
79	501.5
80	366.0
81	198.0
82	220.5
83	250.5
84	230.5
85	198.0
86	162.5
87	147.5
88	143.0
89	159.5
90	175.5
91	160.0
92	114.0
93	64.0
94	47.0
95	28.5
96	6.5
97	9.0
98	13.0
99	10.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	4.713646005185011E-5
105-109	9.427292010370022E-5
110-114	4.713646005185011E-5
115-119	1.4140938015555032E-4
120-124	1.8854584020740043E-4
125-129	3.7709168041480086E-4
130-134	4.2422814046665097E-4
135-139	3.2995522036295075E-4
140-144	2.8281876031110065E-4
145-149	4.713646005185011E-4
150-151	5.892057506481263E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	424300.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.089087909497996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.26617399196162	5.38675465472543
2	17.23842862699339	3.1336318642469947
3	8.722935304032154	2.3785057742163564
4	5.0667703876572014	1.8420928588263021
5	2.828990016854661	1.2856469479142116
6	1.7476986905224943	0.9530992222484093
7	1.0968494749124855	0.6978552910676408
8	0.6456631660832362	0.46947914211642705
9	0.46933748217295473	0.3839264671223191
>10	2.4789316737974847	4.061748762667924
>50	0.2437443277583301	1.5866132453452746
>100	0.15298846103980293	2.5981616780579775
>500	0.0259302476338649	1.5366485976903135
>1k	0.01296512381693245	2.245580956870139
>5k	0.0	0.0
>10k+	0.00259302476338649	71.44025453688428
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC	303121	71.44025453688428	TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGACATCTCGTATGC	2132	0.5024746641527221	TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGAGATCTCGTATGC	2018	0.4756068819231676	TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGAAATCTCGTATGC	1855	0.4371906669809097	TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATATCGTATGC	1767	0.4164506245580957	TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGCTATCTCGTATGC	1756	0.4138581192552439	TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGCC	876	0.20645769502710345	TruSeq Adapter, Index 7 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATCTCGTATGCCG	803	0.18925288710817817	TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGGTATCTCGTATGC	720	0.1696912561866604	TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGATATCTCGTATGC	677	0.1595569172755126	TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGATATCTCGTATGC	647	0.15248644826773508	TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTATATCTCGTATGC	601	0.14164506245580957	TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAATATCTCGTATGC	586	0.1381098279519208	TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACG	584	0.1376384633514023	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGCATGC	520	0.12255479613481027	TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGATATCTCGTATGC	506	0.11925524393118078	TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCGATATCTCGTATGC	466	0.10982795192081074	TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGACTCCAGTCACCACGATATCTCGTATGCC	457	0.1077068112184775	RNA PCR Primer, Index 31 (100% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCATATCTCGTATGC	453	0.10676408201744049	TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTTATCTCGTATGC	439	0.10346452981381098	TruSeq Adapter, Index 7 (97% over 35bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.014612302616073533	0.0	0.0	0.0	0.0
2	0.014847984916332784	0.0	0.0	0.0	0.0
3	0.014847984916332784	0.0	0.0	0.0	0.0
4	0.014847984916332784	0.0	0.0	0.0	0.0
5	0.015319349516851285	0.0	0.0	0.0	0.0
6	0.015555031817110534	0.0	0.0	0.0	0.0
7	0.016262078717888286	0.0	0.0	0.0	0.0
8	0.01791185481970304	0.0	0.0	0.0	0.0
9	0.018147537119962292	0.0	0.0	0.0	0.0
10-14	0.026537827009191613	0.0	0.0	0.0	0.0
15-19	0.032477020975724726	0.0	0.0	0.0	0.0
20-24	0.033561159556917276	0.0	0.0	0.0	0.0
25-29	0.03591798255950978	0.0	0.0	0.0	0.0
30-34	0.041055856705161445	0.0	0.0	0.0	0.0
35-39	0.0447796370492576	0.0	0.0	0.0	0.0
40-44	0.050058920575064815	0.0	0.0	0.0	0.0
45-49	0.05637520622201273	0.0	0.0	0.0	0.0
50-54	0.06919632335611595	0.0	0.0	0.0	0.0
55-59	0.08460994579307093	0.0	0.0	0.0	0.0
60-64	0.10200329955220362	0.0	0.0	0.0	0.0
65-69	0.11496582606646241	0.0	0.0	0.0	0.0
70-74	0.12302616073532877	0.0	0.0	0.0	0.0
75-79	0.13759132689135048	0.0	0.0	0.0	0.0
80-84	0.15833136931416453	0.0	0.0	0.0	0.0
85-89	0.180815460758897	0.0	0.0	0.0	0.0
90-94	0.21682771623851047	0.0	0.0	0.0	0.0
95-99	0.2611359886872496	0.0	0.0	0.0	0.0
100-104	0.3177940136695734	0.0	0.0	0.0	0.0
105-109	0.39915154371906664	0.0	0.0	0.0	0.0
110-114	0.4986094744284705	0.0	0.0	0.0	0.0
115-119	0.6222484091444732	0.0	0.0	0.0	0.0
120-124	0.7820410087202451	0.0	0.0	0.0	0.0
125-129	0.9695969832665566	0.0	1.8854584020740043E-4	0.0	0.0
130-134	1.2140938015555034	0.0	2.3568230025925054E-4	3.299552203629507E-4	0.0
135-139	1.485552674994108	0.0	2.3568230025925054E-4	4.713646005185011E-4	0.0
140	1.655668159321235	0.0	2.3568230025925054E-4	4.713646005185011E-4	0.0
>>END_MODULE