##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename DRR051444.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 374342 Sequences flagged as poor quality 0 Sequence length 151 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.82234694477243 34.0 34.0 34.0 34.0 34.0 2 33.78868788434106 34.0 34.0 34.0 34.0 34.0 3 33.79342419498747 34.0 34.0 34.0 34.0 34.0 4 33.82670392315049 34.0 34.0 34.0 34.0 34.0 5 33.8471077250215 34.0 34.0 34.0 34.0 34.0 6 37.56775355156515 38.0 38.0 38.0 37.0 38.0 7 37.48607957429303 38.0 38.0 38.0 37.0 38.0 8 37.47512969423682 38.0 38.0 38.0 37.0 38.0 9 37.52139487420594 38.0 38.0 38.0 37.0 38.0 10-14 37.434714245262356 38.0 38.0 38.0 37.0 38.0 15-19 37.42164277585737 38.0 38.0 38.0 37.0 38.0 20-24 37.406928423740865 38.0 38.0 38.0 37.0 38.0 25-29 37.36997130965801 38.0 38.0 38.0 37.0 38.0 30-34 37.27683188100721 38.0 38.0 38.0 36.8 38.0 35-39 37.07258870230966 38.0 38.0 38.0 36.0 38.0 40-44 36.92151935930246 38.0 38.0 38.0 35.6 38.0 45-49 37.0821996997398 38.0 38.0 38.0 36.0 38.0 50-54 37.00746643443696 38.0 38.0 38.0 36.0 38.0 55-59 36.96443305854005 38.0 38.0 38.0 36.0 38.0 60-64 36.85280465456721 38.0 38.0 38.0 35.6 38.0 65-69 36.83179712669163 38.0 38.0 38.0 35.6 38.0 70-74 36.52745297081278 38.0 37.8 38.0 33.8 38.0 75-79 35.18598233700733 38.0 37.0 38.0 25.0 38.0 80-84 35.19114018731534 38.0 37.8 38.0 25.0 38.0 85-89 35.12749036976882 38.0 37.0 38.0 25.0 38.0 90-94 35.07611649240534 38.0 37.0 38.0 25.0 38.0 95-99 34.954084767405206 38.0 37.0 38.0 24.6 38.0 100-104 34.86913143595963 38.0 37.0 38.0 24.2 38.0 105-109 34.76272552906166 38.0 37.0 38.0 24.0 38.0 110-114 34.69936475201821 38.0 37.0 38.0 23.2 38.0 115-119 34.58212436755694 38.0 37.0 38.0 22.8 38.0 120-124 34.46275491395569 38.0 36.8 38.0 22.2 38.0 125-129 34.336641894310546 38.0 36.2 38.0 21.8 38.0 130-134 34.12780772662431 38.0 36.0 38.0 20.8 38.0 135-139 34.0369079611692 38.0 36.0 38.0 19.8 38.0 140-144 33.88497256519439 38.0 35.4 38.0 19.2 38.0 145-149 33.65265826436788 38.0 34.8 38.0 18.2 38.0 150-151 30.724305581527055 35.0 28.0 37.5 14.5 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.12601316243164717 1101 2 -0.20107581161135357 1101 3 -0.08141991381431524 1101 4 0.0062213645854711785 1101 5 -0.037221581361819744 1101 6 -0.0016685592713940878 1101 7 0.013247617544109858 1101 8 0.07562494116305629 1101 9 -0.004479791600765282 1101 10-14 0.09942662592286666 1101 15-19 0.03595975069637092 1101 20-24 0.028825738709947757 1101 25-29 0.002488080670715931 1101 30-34 0.004461017575266624 1101 35-39 0.10137996059334142 1101 40-44 0.0654131718488884 1101 45-49 -0.04293291312230707 1101 50-54 -0.0010995644607874056 1101 55-59 0.07085808172469399 1101 60-64 0.11007638164308986 1101 65-69 0.1128855758122711 1101 70-74 0.0847713272650239 1101 75-79 0.0796562038279447 1101 80-84 0.05427272152486751 1101 85-89 0.08695630012745426 1101 90-94 0.0598554937374729 1101 95-99 0.13048686100395912 1101 100-104 0.15033882591735903 1101 105-109 0.07736082801599053 1101 110-114 0.12359486193003733 1101 115-119 0.09181837317844099 1101 120-124 0.11593557683258382 1101 125-129 0.12032951272361458 1101 130-134 0.07233495866132245 1101 135-139 0.02167735486690958 1101 140-144 0.10967077198159814 1101 145-149 0.0017228506158275536 1101 150-151 -0.006813013601522755 1102 1 -0.028548527715130945 1102 2 -0.02742946831018145 1102 3 -0.016462166682373436 1102 4 0.05261354538988172 1102 5 0.041576056959812036 1102 6 0.05192322431355478 1102 7 0.09616524860141595 1102 8 -0.043448161901480375 1102 9 -0.01115775403493302 1102 10-14 0.09155256149448121 1102 15-19 -0.061102834553182106 1102 20-24 0.04223305481240658 1102 25-29 0.05041774809602373 1102 30-34 -0.04452332935033354 1102 35-39 0.14131674551136086 1102 40-44 4.210350941065144E-4 1102 45-49 0.01599138720342097 1102 50-54 0.05715297901889471 1102 55-59 -0.016970889793746835 1102 60-64 0.1409829925650783 1102 65-69 0.07355080249397616 1102 70-74 0.12899947343250773 1102 75-79 0.3239604708459751 1102 80-84 0.3001822884088199 1102 85-89 0.231898127568833 1102 90-94 0.31387444280605337 1102 95-99 0.18138099119766338 1102 100-104 0.2961684079482012 1102 105-109 0.2972575432274098 1102 110-114 0.38522744165405953 1102 115-119 0.5293092444723797 1102 120-124 0.40492799952355796 1102 125-129 0.4735756612790567 1102 130-134 0.763487358786513 1102 135-139 0.770209420292197 1102 140-144 0.5282796151378761 1102 145-149 0.737742889333127 1102 150-151 1.037180587610223 1103 1 0.05441093698693322 1103 2 0.06983335237499233 1103 3 -0.06794464543139611 1103 4 0.021082783811792183 1103 5 0.03695807515902061 1103 6 0.03149808958640676 1103 7 0.12768544711029506 1103 8 0.014164738102742547 1103 9 0.03885261697846687 1103 10-14 0.04925188661038504 1103 15-19 0.05438300901351312 1103 20-24 0.03417977085021562 1103 25-29 -0.06715747055951482 1103 30-34 -0.06355644361201485 1103 35-39 -0.012890236869438354 1103 40-44 -0.2672936160749373 1103 45-49 -0.2636989036074695 1103 50-54 -0.0930235202647296 1103 55-59 -0.06043177378820275 1103 60-64 0.049597463585776325 1103 65-69 0.009815163466704746 1103 70-74 -0.05031073894279814 1103 75-79 -0.3745407256616815 1103 80-84 -0.45276576817850156 1103 85-89 -0.5467483399487705 1103 90-94 -0.4459035022736657 1103 95-99 -0.4624847464310591 1103 100-104 -0.3917249703517953 1103 105-109 -0.4532402654158645 1103 110-114 -0.3599312156453962 1103 115-119 -0.1971280051348714 1103 120-124 -0.3310016081832643 1103 125-129 -0.3094160687980363 1103 130-134 -0.32772013446272297 1103 135-139 -0.3895788958009234 1103 140-144 -0.3461944400436039 1103 145-149 -0.2819526253956326 1103 150-151 0.009397795042019652 1104 1 -0.03566852895777828 1104 2 -0.02744888473026208 1104 3 0.038963946072662736 1104 4 -0.02160183707476193 1104 5 0.011727194373705174 1104 6 0.0011609356612822808 1104 7 0.07069478874291946 1104 8 0.04839538835209822 1104 9 0.08393028001369629 1104 10-14 0.08833797898633833 1104 15-19 0.004503695577220412 1104 20-24 0.0337628357175177 1104 25-29 -0.04043362309156606 1104 30-34 0.020846320455113698 1104 35-39 -0.016787279900846386 1104 40-44 -0.025345331006384697 1104 45-49 -0.044932290732532465 1104 50-54 0.12358354201741406 1104 55-59 0.040881887142653284 1104 60-64 0.06109529713881301 1104 65-69 0.06685291422382988 1104 70-74 0.09697869009645643 1104 75-79 0.2180306961541163 1104 80-84 -0.3163942860686859 1104 85-89 0.13161930777650355 1104 90-94 0.17407194663109493 1104 95-99 0.1847784763829381 1104 100-104 0.2303044160875629 1104 105-109 0.2952075575955604 1104 110-114 0.27889419375298274 1104 115-119 0.32320782862702657 1104 120-124 0.23947796573898472 1104 125-129 0.15217604805414453 1104 130-134 0.3734126444020518 1104 135-139 0.42623705374126075 1104 140-144 0.30211210579857806 1104 145-149 0.43399435527285135 1104 150-151 1.2670379934211695 1105 1 -0.035097811097017484 1105 2 0.07644985046266584 1105 3 -0.011370560793004358 1105 4 0.02319700744724429 1105 5 -0.018415535242439773 1105 6 -0.024980750815011277 1105 7 -0.07202981230623351 1105 8 -0.03239679612317303 1105 9 -0.038441057712859106 1105 10-14 -0.05247887975847476 1105 15-19 0.09915018149984434 1105 20-24 0.03902948834191733 1105 25-29 -0.0474597717991756 1105 30-34 0.0999417978420567 1105 35-39 0.191542191808999 1105 40-44 0.13381915409377143 1105 45-49 0.16337050066958625 1105 50-54 0.12376268122643097 1105 55-59 0.18180606892983064 1105 60-64 0.23040832936580813 1105 65-69 0.2111977923068551 1105 70-74 0.15359169524194272 1105 75-79 0.05935769040682004 1105 80-84 -0.15933533863692162 1105 85-89 0.18432876323745262 1105 90-94 0.0263557207913081 1105 95-99 0.11517075714804292 1105 100-104 0.22151596284329855 1105 105-109 0.2001727026822735 1105 110-114 0.25065399074190964 1105 115-119 0.06689913947266746 1105 120-124 0.15442274953822732 1105 125-129 0.1161732797214654 1105 130-134 0.24370341963550857 1105 135-139 0.3179639517041224 1105 140-144 0.34563350521080594 1105 145-149 0.1839605535295945 1105 150-151 0.8767617619644881 1106 1 -0.011097492988177748 1106 2 -0.03231156293468729 1106 3 -0.01801704742995014 1106 4 -0.03561535306948116 1106 5 -0.047347534506812394 1106 6 -0.18492090658893545 1106 7 0.04979593363415091 1106 8 0.14667064792197948 1106 9 0.04095095676109395 1106 10-14 -0.2267369157345982 1106 15-19 0.026043997762165816 1106 20-24 0.040381053288314206 1106 25-29 -0.005260645604401759 1106 30-34 0.01501051432257583 1106 35-39 -0.08896340243323664 1106 40-44 0.0010333656063323815 1106 45-49 -0.01717356486084043 1106 50-54 0.04188166387459091 1106 55-59 0.13581250069299955 1106 60-64 0.13700342154281486 1106 65-69 0.26447784240900063 1106 70-74 0.20166012840678604 1106 75-79 -0.01391550047339507 1106 80-84 0.07251868916777937 1106 85-89 0.04881399851372947 1106 90-94 0.0136083041830517 1106 95-99 -0.09515127835091874 1106 100-104 -0.10776247683282492 1106 105-109 -0.09718510515016732 1106 110-114 0.12437829972500936 1106 115-119 0.034249839560651196 1106 120-124 0.023520563036974806 1106 125-129 -0.014871459305027201 1106 130-134 0.00892855619864008 1106 135-139 0.1384577162737557 1106 140-144 0.12365596170693038 1106 145-149 0.24790599786302892 1106 150-151 0.7021692146582197 1107 1 -0.05494358830653567 1107 2 -0.041474088760075745 1107 3 -0.04303182569741182 1107 4 0.011379537454281774 1107 5 0.02030766656541516 1107 6 0.004278959967699336 1107 7 0.01661360200397155 1107 8 0.023551584834869743 1107 9 -0.07116780783427146 1107 10-14 -0.027954879084504114 1107 15-19 0.012100336614672358 1107 20-24 0.048149447110120036 1107 25-29 0.13748863889334473 1107 30-34 0.05319346049456897 1107 35-39 -0.03443364397742954 1107 40-44 -0.02610948317541073 1107 45-49 0.03746622584378656 1107 50-54 0.009052840107621307 1107 55-59 -0.01871725776280897 1107 60-64 0.060165795195949556 1107 65-69 0.07664752618334347 1107 70-74 0.14389905055897145 1107 75-79 0.17398387854672848 1107 80-84 0.36426451467302456 1107 85-89 0.2667065297821978 1107 90-94 0.2517461839552553 1107 95-99 0.2962065078938778 1107 100-104 0.2760826820408653 1107 105-109 0.3697519599151846 1107 110-114 0.32081980997615034 1107 115-119 0.3857501995065675 1107 120-124 0.43358324630212053 1107 125-129 0.6466073038856806 1107 130-134 0.5040590439107362 1107 135-139 0.4877164617890628 1107 140-144 0.29215923048411696 1107 145-149 0.33822140413370505 1107 150-151 0.5708416287540601 1108 1 -0.012164229709192398 1108 2 -0.01723992638940075 1108 3 0.027658588980003174 1108 4 -0.007143831556625457 1108 5 0.0538461757268891 1108 6 -0.048578619433754966 1108 7 -0.00419567178471425 1108 8 0.0032522741780312003 1108 9 0.06164286289622112 1108 10-14 0.04448885630615251 1108 15-19 0.11979343802391185 1108 20-24 0.12438119658130375 1108 25-29 0.1532530478321803 1108 30-34 0.12627891602856067 1108 35-39 0.20788972423976304 1108 40-44 0.21333666114613692 1108 45-49 0.14591137405057708 1108 50-54 0.1687439254758445 1108 55-59 0.17893254638754286 1108 60-64 0.1805738450731198 1108 65-69 0.15811264827684823 1108 70-74 0.12509589632785634 1108 75-79 0.19500150303131392 1108 80-84 0.23807384005331045 1108 85-89 0.3121837322474903 1108 90-94 0.38717612057696726 1108 95-99 0.27016544891674243 1108 100-104 0.4147315376994669 1108 105-109 0.3250613309341972 1108 110-114 0.3352908571665907 1108 115-119 0.36571757774046887 1108 120-124 0.35743721826009534 1108 125-129 0.4059297077367887 1108 130-134 0.49435466378381676 1108 135-139 0.5549157125202768 1108 140-144 0.5027775756636785 1108 145-149 0.376927804270224 1108 150-151 1.1051956820680218 1109 1 -0.028670488929513738 1109 2 0.044609606111521316 1109 3 -0.04205255652895801 1109 4 0.009473424638521522 1109 5 0.011016479394434953 1109 6 -0.0832429746725083 1109 7 -0.08993780764905068 1109 8 -0.04831490220019674 1109 9 -0.017344602518129193 1109 10-14 0.0203678009872732 1109 15-19 0.07985349747792014 1109 20-24 0.04175847933499455 1109 25-29 -0.014578212548556735 1109 30-34 0.09496868755641685 1109 35-39 0.12644801480562506 1109 40-44 0.06272506781482434 1109 45-49 0.007210765181717704 1109 50-54 0.16546507483943174 1109 55-59 0.14019379492187767 1109 60-64 0.1851434896204509 1109 65-69 0.07291621390331215 1109 70-74 0.15243788204355724 1109 75-79 0.10764264577543514 1109 80-84 0.024968124516362877 1109 85-89 0.018856668561454626 1109 90-94 0.11410802292384403 1109 95-99 0.1467360056573881 1109 100-104 0.15400085470481883 1109 105-109 0.1730126294978973 1109 110-114 0.09853955698839201 1109 115-119 0.2688539486237431 1109 120-124 0.21300573467453887 1109 125-129 0.29968804512319736 1109 130-134 0.2606596148543332 1109 135-139 0.2860079832963862 1109 140-144 0.39457792641411515 1109 145-149 0.49447319698761305 1109 150-151 0.5381192011161104 1110 1 -0.15744586979848663 1110 2 -0.03969305310992155 1110 3 -0.025250907801677158 1110 4 -0.07074534111146846 1110 5 -0.019690567118239244 1110 6 -0.2174555626853092 1110 7 0.020237494376431187 1110 8 -0.01981148149030787 1110 9 -0.03728102871213679 1110 10-14 -0.024478179740974326 1110 15-19 -0.0120875994446763 1110 20-24 -0.07698641205559653 1110 25-29 -0.06008208735705978 1110 30-34 -0.05905417705731253 1110 35-39 -0.038800566759967126 1110 40-44 -0.07235400023508731 1110 45-49 3.2942464268614913E-4 1110 50-54 0.038475549048129665 1110 55-59 0.005450660044608924 1110 60-64 0.011258850628841799 1110 65-69 -0.1184874498241939 1110 70-74 -0.05471851251324722 1110 75-79 -0.2149701677896374 1110 80-84 -0.12057636966269314 1110 85-89 -0.12241590537926328 1110 90-94 -0.12086295967569782 1110 95-99 -0.19213992561902415 1110 100-104 -0.06979031981376238 1110 105-109 -0.11982029714272358 1110 110-114 0.04061373973564031 1110 115-119 -0.14231009075808743 1110 120-124 -0.07081488140255487 1110 125-129 0.04415447925145344 1110 130-134 -0.009503910547735472 1110 135-139 0.012391312388139397 1110 140-144 0.11278568401527878 1110 145-149 0.10744864073177496 1110 150-151 0.585086970094693 1111 1 0.05671756499604186 1111 2 0.019048671338701695 1111 3 0.023378974283957632 1111 4 -0.008953904593269613 1111 5 -0.0010837978151485572 1111 6 0.07106443574772214 1111 7 0.07563765890810004 1111 8 0.12297850558220347 1111 9 0.13463791985213192 1111 10-14 0.08698517278772044 1111 15-19 0.058099653268129714 1111 20-24 0.13053386059689132 1111 25-29 0.10285578234952197 1111 30-34 0.19033450943149433 1111 35-39 0.23402408948636122 1111 40-44 0.24085808892541394 1111 45-49 -0.16513165578196265 1111 50-54 -0.07204292159080694 1111 55-59 -0.010731421672794283 1111 60-64 0.12198807850521831 1111 65-69 0.13550812349033237 1111 70-74 0.4203449497032281 1111 75-79 0.7040142408757006 1111 80-84 0.6295237204379873 1111 85-89 0.7334974412732933 1111 90-94 0.7208783338566391 1111 95-99 0.5671794225221518 1111 100-104 0.7388566030522199 1111 105-109 0.8004431494040674 1111 110-114 0.7920812055561441 1111 115-119 0.7990486666360468 1111 120-124 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-0.08087743317446439 2119 110-114 -0.20824509760168297 2119 115-119 -0.16739676594166752 2119 120-124 -0.3250489303644244 2119 125-129 -0.2958706560324629 2119 130-134 -0.4017543493765672 2119 135-139 -0.5680361951680268 2119 140-144 -0.5818773266100834 2119 145-149 -0.28289795613773805 2119 150-151 -0.8901038814928839 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 3.0 16 4.0 17 16.0 18 60.0 19 125.0 20 271.0 21 669.0 22 1300.0 23 1794.0 24 3899.0 25 16041.0 26 10456.0 27 3757.0 28 2736.0 29 2929.0 30 3345.0 31 4319.0 32 5816.0 33 7742.0 34 11529.0 35 18631.0 36 37363.0 37 241536.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.8853288724754 9.73202785542117 6.1239705198574095 50.258672752246014 2 21.91471969482452 22.28737357817183 41.211779602609376 14.586127124394272 3 20.021531113260068 18.117657115685656 31.227594018304117 30.63321775275016 4 25.591571343851342 27.833104487340453 16.562127680035903 30.013196488772298 5 36.53103311944692 25.778566124025627 18.214627265976034 19.475773490551422 6 27.858749485764356 30.650848689166594 20.33247671914987 21.157925105919187 7 16.054837554963107 22.127626608822947 36.08224564702866 25.73529018918529 8 18.452110636797368 24.094277425455868 24.314396995261017 33.13921494248575 9 31.52090868777749 15.127877716099183 26.065736679293266 27.285476916830063 10-14 25.28896036244931 24.813031933365746 21.376228155002643 28.5217795491823 15-19 26.455501362658406 21.669714683211975 22.44996417712369 29.424819777005933 20-24 27.267340845901177 24.16792576619857 21.58803616803476 26.976697219865493 25-29 27.43632855847611 21.89825285211834 21.645698861824062 29.019719727581496 30-34 25.598344574667248 21.89345950982655 23.56624907034596 28.941946845160242 35-39 29.648250603079106 21.52870870277216 21.408600455748818 27.414440238399912 40-44 25.558968528283298 22.093996795284504 23.41408525355348 28.932949422878718 45-49 29.529721207238214 21.628625024510107 23.212552165785016 25.629101602466665 50-54 27.54967588050859 19.993192742630104 21.376711699292343 31.080419677568965 55-59 27.310113495382073 19.348820111796318 25.2372546107165 28.103811782105108 60-64 27.257033125094843 21.229960696142065 23.171750806274083 28.34125537248901 65-69 26.372604127483502 28.516813852887868 19.207534709161166 25.903047310467464 70-74 26.05488940585098 28.46499659652771 19.04752999810859 26.432583999512723 75-79 26.66508508229315 25.707531921555553 20.25031778625208 27.37706520989922 80-84 27.033323199287874 23.48225749487465 21.293196814027084 28.191222491810393 85-89 28.2408330025266 23.941342756561326 20.283252488263262 27.534571752648812 90-94 27.838933131015764 22.9877460736411 21.148401539821922 28.024919255521212 95-99 27.660516952191035 23.3983571194607 20.454092480292218 28.48703344805605 100-104 27.196113016617357 23.456392998684997 20.958846942815754 28.388647041881892 105-109 27.30772128503026 23.55354479775195 21.23250524047964 27.90622867673815 110-114 27.9495648075765 24.081591432621718 20.64857002860317 27.320273731198615 115-119 27.237300860012237 24.834503081777303 20.39989633900352 27.528299719206938 120-124 27.649141704856756 24.9339721590396 20.493663304229255 26.923222831874387 125-129 28.42750951414419 25.000868200719456 19.73909099117534 26.832531293961008 130-134 27.113450588600497 25.021438383281424 20.34164635029223 27.52346467782585 135-139 27.62555455163802 25.659491001342733 19.755369459759976 26.959584987259273 140-144 27.283010903222877 25.156121006804828 20.219453743457667 27.341414346514632 145-149 26.771049751626613 25.646476175819288 20.159390737083303 27.4230833354708 150-151 27.023351864863205 26.265304541337958 19.67570267981417 27.035640913984672 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.5 2 2.5 3 1.5 4 1.0 5 1.5 6 1.0 7 1.0 8 1.0 9 2.5 10 5.0 11 3.0 12 4.0 13 7.0 14 7.5 15 14.5 16 19.0 17 21.0 18 31.0 19 46.0 20 69.0 21 95.0 22 160.5 23 213.0 24 236.0 25 301.5 26 400.5 27 511.5 28 668.0 29 816.0 30 917.0 31 1068.0 32 1233.5 33 1399.0 34 1720.5 35 2194.0 36 2869.5 37 3919.0 38 5227.0 39 6468.5 40 7654.0 41 8709.5 42 9288.5 43 9485.0 44 10326.5 45 10460.5 46 11262.5 47 12561.5 48 12826.0 49 12264.5 50 11461.5 51 10889.5 52 10223.0 53 10403.5 54 11346.5 55 12346.0 56 12027.5 57 10044.0 58 9732.0 59 11981.5 60 10965.0 61 8003.5 62 7893.5 63 7152.0 64 6861.0 65 6887.5 66 6698.5 67 6657.0 68 5557.0 69 5277.5 70 4262.0 71 3492.0 72 3199.5 73 3475.5 74 6219.5 75 6256.0 76 3551.5 77 3374.5 78 3441.0 79 3088.5 80 2093.0 81 938.0 82 962.5 83 1184.0 84 1285.5 85 1183.0 86 910.0 87 942.0 88 1023.0 89 1178.5 90 1186.5 91 1148.5 92 1018.5 93 560.5 94 265.0 95 110.5 96 47.5 97 29.5 98 9.5 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03312478963087231 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 5.342708004979404E-5 20-24 1.0685416009958808E-4 25-29 0.0016562394815436152 30-34 0.0024576456822905258 35-39 0.0034727602032366125 40-44 0.004968718444630846 45-49 0.0017630936416432033 50-54 0.009510020248863339 55-59 0.0049152913645810516 60-64 0.007372937046871577 65-69 0.008441478647867458 70-74 0.0049152913645810516 75-79 0.009029176528415192 80-84 0.00699894748652302 85-89 0.0012288228411452629 90-94 0.0034727602032366125 95-99 0.012875926292000362 100-104 0.01244850965160201 105-109 0.011807384691004483 110-114 0.012769072131900774 115-119 0.01202109301120366 120-124 0.0080674890875189 125-129 0.0012288228411452629 130-134 0.0035796143633362006 135-139 0.008174343247618487 140-144 0.01308963461219954 145-149 0.01784464473663121 150-151 0.006678385006224255 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 374342.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.07359580276859 #Duplication Level Percentage of deduplicated Percentage of total 1 79.98529740786908 20.055190173691436 2 7.110514484184059 3.5657233225232545 3 3.2239162165329587 2.4250551634601516 4 1.7717688922981856 1.7769846824561497 5 1.1229371091294575 1.4078035593120728 6 0.7606993319909228 1.1444080546665882 7 0.5763842277410213 1.01164176074285 8 0.494348025271412 0.9916066057241774 9 0.4037885809867783 0.9111988502492374 >10 3.4433896932698356 18.10056045006972 >50 0.6008885479592163 10.595925650875403 >100 0.4677128945994609 22.327711023609425 >500 0.03089675157946325 5.120184216572012 >1k 0.006392431361268258 2.743213425156675 >5k 0.0 0.0 >10k+ 0.001065405226878043 7.8227930608908425 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC 29284 7.8227930608908425 TruSeq Adapter, Index 3 (100% over 50bp) CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG 3014 0.8051460963503962 No Hit CTCTTATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG 2048 0.547093299709891 No Hit CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT 1534 0.40978570398192027 No Hit CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCA 1283 0.34273471851942877 No Hit CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCACTCCGC 1268 0.3387276875156942 No Hit CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA 1122 0.29972591907934454 No Hit GTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGG 991 0.2647311816467295 No Hit CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA 984 0.26286123384498666 No Hit CTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT 862 0.23027071501461233 No Hit CTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTC 792 0.21157123699718441 No Hit CTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGA 791 0.21130410159693544 No Hit CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG 790 0.21103696619668647 No Hit CTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGT 771 0.20596139359195603 No Hit CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG 747 0.19955014398598073 No Hit GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA 742 0.19821446698473588 No Hit CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA 697 0.18619337397353222 No Hit CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA 690 0.18432342617178943 No Hit CTAATACATGCCGACGGGCGCTGACCCCCCTCGCGGGGGGGATGCGTGCA 658 0.17577509336382238 No Hit CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC 640 0.17096665615934092 No Hit CTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGG 633 0.16909670835759813 No Hit CACGAACGTGCGTTGCGTGACGGGCGAGGGGGCGGCCCCCTTTCCGGCCG 627 0.1674938959561043 No Hit CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG 618 0.16508967735386357 No Hit CTCCCTTTCGATCGGCTGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG 612 0.16348686495236978 No Hit CGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGA 611 0.1632197295521208 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAACTCGTATGC 585 0.15627420914564757 TruSeq Adapter, Index 3 (98% over 50bp) CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCAACCGATCCCGG 583 0.15573993834514963 No Hit GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC 575 0.15360285514315786 No Hit CTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGAT 543 0.1450545223351908 No Hit GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT 536 0.14318457453344802 No Hit CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC 521 0.13917754352971348 No Hit CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG 520 0.1389104081294645 No Hit ATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGG 517 0.1381090019287176 No Hit CTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAA 515 0.13757473112821966 No Hit CTACGAATGGTTTAGCACCAGGTTCCCCACGAACGTGCGTTGCGTGACGG 512 0.13677332492747274 No Hit CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT 504 0.13463624172548097 No Hit CTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGC 500 0.1335677001244851 No Hit CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT 497 0.1327662939237382 No Hit CGCTGACCCCCCTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCA 497 0.1327662939237382 No Hit CGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCACGCGAACCCACGCAGT 487 0.1300949399212485 No Hit GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCC 487 0.1300949399212485 No Hit AAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGA 476 0.12715645051850982 No Hit GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG 470 0.125553638117016 No Hit CTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCC 460 0.12288228411452629 No Hit CGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCT 458 0.12234801331402834 No Hit CTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTC 450 0.12021093011203658 No Hit GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG 448 0.11967665931153863 No Hit CGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCAATGAAGGTGAAG 447 0.11940952391128969 No Hit GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC 434 0.11593676370805306 No Hit CTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGG 426 0.1137996805060613 No Hit CCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGG 425 0.11353254510581233 No Hit CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT 420 0.11219686810456748 No Hit GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCA 419 0.11192973270431851 No Hit CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT 416 0.1111283265035716 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAGCTCGTATGC 413 0.11032692030282468 TruSeq Adapter, Index 3 (98% over 50bp) ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG 412 0.11005978490257573 No Hit CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGG 404 0.10792270170058395 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCACCTCGTATGC 400 0.10685416009958808 TruSeq Adapter, Index 3 (98% over 50bp) CTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTC 389 0.1039156706968494 No Hit CGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAG 389 0.1039156706968494 No Hit CTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAAC 387 0.10338139989635148 No Hit CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT 387 0.10338139989635148 No Hit GTTTCCCTCAGGATAGCTGGCGCTCTCGCACGCGAACCCACGCAGTTTTA 382 0.10204572289510662 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0016028124014938212 0.0 0.0 0.0 0.0 2 0.0016028124014938212 0.0 0.0 0.0 0.0 3 0.0024042186022407317 0.0 0.0 0.0 0.0 4 0.002938489402738672 0.0 0.0 0.0 0.0 5 0.002938489402738672 0.0 0.0 0.0 0.0 6 0.002938489402738672 0.0 0.0 0.0 0.0 7 0.002938489402738672 0.0 0.0 0.0 0.0 8 0.0032056248029876423 0.0 0.0 0.0 0.0 9 0.0032056248029876423 0.0 0.0 0.0 0.0 10-14 0.0036864685234357887 0.0 0.0 0.0 0.0 15-19 0.005128999684780228 0.0 0.0 0.0 0.0 20-24 0.008655186968066634 0.0 0.0 0.0 0.0 25-29 0.013517051252597893 0.0 0.0 0.0 0.0 30-34 0.016989811455834506 0.0 0.0 0.0 0.0 35-39 0.019500884218174825 0.0 0.0 0.0 0.0 40-44 0.022439373620913496 0.0 0.0 0.0 0.0 45-49 0.028102644106191666 0.0 0.0 0.0 0.0 50-54 0.034300185391967775 0.0 0.0 0.0 0.0 55-59 0.04327593484033317 0.0 0.0 0.0 0.0 60-64 0.05989175673581911 0.0 0.0 0.0 0.0 65-69 0.080674890875189 0.0 0.0 0.0 0.0 70-74 0.10925837870182882 0.0 0.0 0.0 0.0 75-79 0.15178633442146489 0.0 0.0 0.0 0.0 80-84 0.22102783016599795 0.0 0.0 0.0 0.0 85-89 0.34049078115733744 0.0 0.0 0.0 0.0 90-94 0.5228908324473343 0.0 0.0 0.0 0.0 95-99 0.8291882823728034 0.0 0.0 0.0 0.0 100-104 1.292347639324468 0.0 0.0 0.0 0.0 105-109 1.951530952978827 0.0 0.0 0.0 0.0 110-114 2.8337188987610262 0.0 0.0 0.0 0.0 115-119 4.018838388425557 0.0 0.0 0.0 0.0 120-124 5.561011054062862 0.0 0.0 0.0 0.0 125-129 7.641034134561442 0.0 0.0 0.0 0.0 130-134 10.23897932906273 0.0 0.0 0.0 0.0 135-139 13.024934418259239 0.0 0.0 0.0 0.0 140 14.720763366119751 0.0 0.0 0.0 0.0 >>END_MODULE