FastQCFastQC Report
Fri 12 Jan 2024
DRR051444.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR051444.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374342
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC292847.8227930608908425TruSeq Adapter, Index 3 (100% over 50bp)
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG30140.8051460963503962No Hit
CTCTTATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG20480.547093299709891No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT15340.40978570398192027No Hit
CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCA12830.34273471851942877No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCACTCCGC12680.3387276875156942No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA11220.29972591907934454No Hit
GTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGG9910.2647311816467295No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA9840.26286123384498666No Hit
CTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT8620.23027071501461233No Hit
CTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTC7920.21157123699718441No Hit
CTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGA7910.21130410159693544No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG7900.21103696619668647No Hit
CTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGT7710.20596139359195603No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG7470.19955014398598073No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA7420.19821446698473588No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA6970.18619337397353222No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6900.18432342617178943No Hit
CTAATACATGCCGACGGGCGCTGACCCCCCTCGCGGGGGGGATGCGTGCA6580.17577509336382238No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC6400.17096665615934092No Hit
CTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGG6330.16909670835759813No Hit
CACGAACGTGCGTTGCGTGACGGGCGAGGGGGCGGCCCCCTTTCCGGCCG6270.1674938959561043No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG6180.16508967735386357No Hit
CTCCCTTTCGATCGGCTGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG6120.16348686495236978No Hit
CGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGA6110.1632197295521208No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAACTCGTATGC5850.15627420914564757TruSeq Adapter, Index 3 (98% over 50bp)
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCAACCGATCCCGG5830.15573993834514963No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC5750.15360285514315786No Hit
CTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGAT5430.1450545223351908No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT5360.14318457453344802No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC5210.13917754352971348No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG5200.1389104081294645No Hit
ATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGG5170.1381090019287176No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAA5150.13757473112821966No Hit
CTACGAATGGTTTAGCACCAGGTTCCCCACGAACGTGCGTTGCGTGACGG5120.13677332492747274No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT5040.13463624172548097No Hit
CTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGC5000.1335677001244851No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT4970.1327662939237382No Hit
CGCTGACCCCCCTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCA4970.1327662939237382No Hit
CGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCACGCGAACCCACGCAGT4870.1300949399212485No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCC4870.1300949399212485No Hit
AAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGA4760.12715645051850982No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG4700.125553638117016No Hit
CTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCC4600.12288228411452629No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCT4580.12234801331402834No Hit
CTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTC4500.12021093011203658No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG4480.11967665931153863No Hit
CGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCAATGAAGGTGAAG4470.11940952391128969No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC4340.11593676370805306No Hit
CTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGG4260.1137996805060613No Hit
CCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGG4250.11353254510581233No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT4200.11219686810456748No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCA4190.11192973270431851No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT4160.1111283265035716No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAGCTCGTATGC4130.11032692030282468TruSeq Adapter, Index 3 (98% over 50bp)
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG4120.11005978490257573No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGG4040.10792270170058395No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCACCTCGTATGC4000.10685416009958808TruSeq Adapter, Index 3 (98% over 50bp)
CTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTC3890.1039156706968494No Hit
CGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAG3890.1039156706968494No Hit
CTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAAC3870.10338139989635148No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT3870.10338139989635148No Hit
GTTTCCCTCAGGATAGCTGGCGCTCTCGCACGCGAACCCACGCAGTTTTA3820.10204572289510662No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)