##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename DRR051442.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 253441 Sequences flagged as poor quality 0 Sequence length 151 %GC 57 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.84397946662142 34.0 34.0 34.0 34.0 34.0 2 33.8133096065751 34.0 34.0 34.0 34.0 34.0 3 33.8309941958878 34.0 34.0 34.0 34.0 34.0 4 33.851965546221805 34.0 34.0 34.0 34.0 34.0 5 33.86360928184469 34.0 34.0 34.0 34.0 34.0 6 37.61866864477334 38.0 38.0 38.0 37.0 38.0 7 37.545184086237036 38.0 38.0 38.0 37.0 38.0 8 37.54470665756527 38.0 38.0 38.0 37.0 38.0 9 37.580150804329215 38.0 38.0 38.0 37.0 38.0 10-14 37.503035420472614 38.0 38.0 38.0 37.0 38.0 15-19 37.490889792890655 38.0 38.0 38.0 37.0 38.0 20-24 37.479661933152094 38.0 38.0 38.0 37.0 38.0 25-29 37.44555932149889 38.0 38.0 38.0 37.0 38.0 30-34 37.37920462750699 38.0 38.0 38.0 37.0 38.0 35-39 37.306869843474416 38.0 38.0 38.0 37.0 38.0 40-44 37.22320855741573 38.0 38.0 38.0 36.8 38.0 45-49 37.238080657825684 38.0 38.0 38.0 36.8 38.0 50-54 37.231686270177285 38.0 38.0 38.0 37.0 38.0 55-59 37.14506729376858 38.0 38.0 38.0 36.0 38.0 60-64 37.09522374043663 38.0 38.0 38.0 36.0 38.0 65-69 36.98551220994235 38.0 38.0 38.0 36.0 38.0 70-74 36.89342450511164 38.0 38.0 38.0 35.8 38.0 75-79 36.72328470926171 38.0 38.0 38.0 35.6 38.0 80-84 36.776602838530465 38.0 38.0 38.0 36.0 38.0 85-89 36.72600486898331 38.0 38.0 38.0 36.0 38.0 90-94 36.68359578757975 38.0 38.0 38.0 36.0 38.0 95-99 36.57805406386497 38.0 38.0 38.0 35.0 38.0 100-104 36.49137274553051 38.0 38.0 38.0 34.6 38.0 105-109 36.38649626540299 38.0 38.0 38.0 34.0 38.0 110-114 36.322330641056496 38.0 38.0 38.0 34.0 38.0 115-119 36.222447038955806 38.0 38.0 38.0 34.0 38.0 120-124 36.103179043643294 38.0 37.6 38.0 34.0 38.0 125-129 35.995501122549236 38.0 37.0 38.0 33.6 38.0 130-134 35.778930007378435 38.0 37.0 38.0 31.2 38.0 135-139 35.69151005559479 38.0 37.0 38.0 30.8 38.0 140-144 35.52959702652688 38.0 37.0 38.0 29.4 38.0 145-149 35.286472196684834 38.0 36.2 38.0 28.0 38.0 150-151 32.06051507056869 36.0 29.5 37.5 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04741678947402761 1101 2 -0.07392257067883179 1101 3 -0.1292152797679691 1101 4 0.007045400765719023 1101 5 0.011063516134214524 1101 6 -0.0015141293070684014 1101 7 0.045616848498504226 1101 8 -0.016141957714246757 1101 9 0.1616178128850052 1101 10-14 0.061986386674163896 1101 15-19 0.06942117074017773 1101 20-24 0.04154258924717169 1101 25-29 0.024647220979453266 1101 30-34 -0.07253301856732008 1101 35-39 0.015519702308807837 1101 40-44 -0.030974201894260034 1101 45-49 -0.10527986029854475 1101 50-54 -0.03438327007338415 1101 55-59 -0.0962435660049934 1101 60-64 0.011488363479386976 1101 65-69 0.0036382888021151416 1101 70-74 0.031230029726927455 1101 75-79 -0.06314969836888906 1101 80-84 0.026446991419810217 1101 85-89 -0.011277862684913487 1101 90-94 0.006999883619904779 1101 95-99 -0.09584352562633569 1101 100-104 -0.028701599957081214 1101 105-109 -0.13312230256541824 1101 110-114 -0.05064261833760497 1101 115-119 -0.15342699943400362 1101 120-124 -0.06419148674727637 1101 125-129 -0.07107400972239475 1101 130-134 -0.04440499458540614 1101 135-139 0.12443844828582229 1101 140-144 -0.08812415934184514 1101 145-149 -0.028920257038954844 1101 150-151 -0.38554392073771737 1102 1 -0.03491059712265354 1102 2 0.021034096796412882 1102 3 -0.053124248387725004 1102 4 -0.062284433622444624 1102 5 -0.048909580211741854 1102 6 0.02345334373414687 1102 7 -0.010051663200179917 1102 8 -0.01596995074576313 1102 9 0.028634374068765567 1102 10-14 0.06587197998793926 1102 15-19 0.02764773479071181 1102 20-24 0.09096636590273022 1102 25-29 0.027802887285936606 1102 30-34 0.0774200102989866 1102 35-39 0.15303530422293932 1102 40-44 0.08594378606525765 1102 45-49 0.026876621938427547 1102 50-54 0.06730724969639112 1102 55-59 0.015551260554644841 1102 60-64 0.2852167247822237 1102 65-69 0.12381294203164828 1102 70-74 0.23103464745247493 1102 75-79 0.23505216724515066 1102 80-84 0.27810906388326373 1102 85-89 0.1612393931423739 1102 90-94 0.18193052952813105 1102 95-99 0.16704575040075298 1102 100-104 0.2755562340782518 1102 105-109 0.2871965718915348 1102 110-114 0.14362337500264744 1102 115-119 0.3698539232290159 1102 120-124 0.4536906223227817 1102 125-129 0.386866758134353 1102 130-134 0.5022058065701245 1102 135-139 0.35536838852443253 1102 140-144 0.45975199564084335 1102 145-149 0.511887734669422 1102 150-151 1.499985441733891 1103 1 -0.03148235135864752 1103 2 -0.06183344227550691 1103 3 -0.07029835680270935 1103 4 -0.02957581259265396 1103 5 -0.006192410487273037 1103 6 0.0819439511298583 1103 7 0.09774299276966048 1103 8 0.03180629265091994 1103 9 -0.0725755950244178 1103 10-14 0.06311228598737983 1103 15-19 -0.05199541200570934 1103 20-24 0.031516551124454395 1103 25-29 -0.08593859141904403 1103 30-34 -0.1789391225564998 1103 35-39 -0.21259149045495462 1103 40-44 -0.206979209923432 1103 45-49 -0.35658494181510747 1103 50-54 -0.10539567220361334 1103 55-59 -0.24476701392421063 1103 60-64 -0.1366631112581942 1103 65-69 -0.1614434092100936 1103 70-74 -0.11493365661802812 1103 75-79 0.07598550934638126 1103 80-84 0.007068095907989402 1103 85-89 -0.08604628669804981 1103 90-94 -0.008739118221818387 1103 95-99 -0.029182302469642707 1103 100-104 -0.10169634696995189 1103 105-109 -0.06329114209982123 1103 110-114 -0.0560552817563007 1103 115-119 -0.013996934928414362 1103 120-124 0.12136778854009123 1103 125-129 0.21924833679330646 1103 130-134 0.22222991827307226 1103 135-139 0.338340265510908 1103 140-144 0.015190938233075713 1103 145-149 -0.0036941245620738528 1103 150-151 0.388078644425633 1104 1 0.037253050710091884 1104 2 0.028423481314753474 1104 3 0.018415356911006597 1104 4 0.04232942597924705 1104 5 0.023754202792673595 1104 6 -0.16129929211338379 1104 7 -0.053157908131240106 1104 8 -0.14386938302475727 1104 9 0.06502033423818432 1104 10-14 -0.009193352984922853 1104 15-19 -0.016810226820531682 1104 20-24 -0.041373005098435556 1104 25-29 -0.13295227176605806 1104 30-34 -0.039149332766712064 1104 35-39 -0.07950006569686252 1104 40-44 -0.22315371776460324 1104 45-49 -0.09263766120116657 1104 50-54 0.14143449129321084 1104 55-59 -0.0491630766536062 1104 60-64 -0.11375687168528259 1104 65-69 -0.129995294428646 1104 70-74 -0.08743115411552083 1104 75-79 -0.02738452735699326 1104 80-84 -0.6272996051263746 1104 85-89 -0.061455028773458764 1104 90-94 -0.06107478722415749 1104 95-99 -0.11989801818535284 1104 100-104 -0.06566031093391445 1104 105-109 0.07308690261156414 1104 110-114 0.06882793479357474 1104 115-119 0.07525898766084538 1104 120-124 -0.0130165958442916 1104 125-129 -0.04351442596945532 1104 130-134 0.03086355190670531 1104 135-139 0.10822681873079176 1104 140-144 -0.09480238842692046 1104 145-149 -0.019818582353188674 1104 150-151 0.17261317896016948 1105 1 -0.048729687190053994 1105 2 -0.004298030757787785 1105 3 -0.006863240270241988 1105 4 -0.049826151427069476 1105 5 0.027332264630324232 1105 6 0.013725289990844658 1105 7 -0.04160431914402096 1105 8 -0.03570812442102067 1105 9 0.09835639891642955 1105 10-14 0.04576130668949929 1105 15-19 0.10916486331385045 1105 20-24 0.07471461644642119 1105 25-29 0.08954308494757868 1105 30-34 0.17928724540437457 1105 35-39 0.06848693310134024 1105 40-44 0.10489992584950869 1105 45-49 0.18221757751838652 1105 50-54 0.06830546255947922 1105 55-59 0.17415987756905338 1105 60-64 0.15296988456948668 1105 65-69 0.18095739867743532 1105 70-74 0.17786710874910483 1105 75-79 0.0776411472852061 1105 80-84 -0.2054488458124908 1105 85-89 -0.015972673261607895 1105 90-94 0.025689115321462452 1105 95-99 0.03894334530202315 1105 100-104 0.1420093055852547 1105 105-109 0.16485736038497123 1105 110-114 0.30183623808212445 1105 115-119 0.09855189708945034 1105 120-124 0.09116236187448834 1105 125-129 -0.10968945055156354 1105 130-134 0.26077997275232434 1105 135-139 0.2870261195064998 1105 140-144 0.4228366370292065 1105 145-149 0.3758030717492815 1105 150-151 1.1047665712905541 1106 1 0.037771310098911215 1106 2 0.05248552436729881 1106 3 -0.04293591189902912 1106 4 0.06516060298334025 1106 5 0.0809802194139877 1106 6 0.08840918698040667 1106 7 0.026344007577570494 1106 8 0.06694187801139151 1106 9 0.03760580076417597 1106 10-14 -0.029187059835209084 1106 15-19 0.13054665029646628 1106 20-24 0.05743962562323901 1106 25-29 0.08954573848807001 1106 30-34 0.1820650600368836 1106 35-39 0.12578571733753563 1106 40-44 0.11591609917877577 1106 45-49 0.2105949817366337 1106 50-54 0.2077189416606302 1106 55-59 0.25608661331622784 1106 60-64 0.21420784965773976 1106 65-69 0.2751067841616859 1106 70-74 0.20981883200252582 1106 75-79 0.08314724379447114 1106 80-84 0.18756703562731758 1106 85-89 0.05896994392536925 1106 90-94 -0.012794297839207047 1106 95-99 0.03443453775889793 1106 100-104 0.05148111835143965 1106 105-109 0.0888183887203624 1106 110-114 0.09942328526649646 1106 115-119 0.11880018002762682 1106 120-124 0.1285043103692658 1106 125-129 0.053053956505976885 1106 130-134 0.2551805600692916 1106 135-139 0.140065529005291 1106 140-144 0.35679311012009407 1106 145-149 0.3985921197031814 1106 150-151 0.6534977366813095 1107 1 -0.0740598471307905 1107 2 0.013582984552513722 1107 3 0.06455343406691583 1107 4 0.012510850593557166 1107 5 0.05919987630174006 1107 6 0.007926397171743815 1107 7 0.16407009252915117 1107 8 0.13737545765545178 1107 9 -0.01397687743610021 1107 10-14 0.021885472249422833 1107 15-19 -0.008772694488790478 1107 20-24 0.07347024330102414 1107 25-29 0.012035162423948975 1107 30-34 0.03878528833283923 1107 35-39 0.10670001501782878 1107 40-44 0.05304783048993755 1107 45-49 0.09477176407354193 1107 50-54 0.07667287479807783 1107 55-59 0.088792548610094 1107 60-64 0.2294832728673768 1107 65-69 0.19970496055640297 1107 70-74 0.18204906161283674 1107 75-79 0.22195599692161494 1107 80-84 0.2821788678984092 1107 85-89 0.22105739945467207 1107 90-94 0.1702599402720466 1107 95-99 0.3340079308543267 1107 100-104 0.207346127897182 1107 105-109 0.2038699268443125 1107 110-114 0.1536466936217522 1107 115-119 0.23983624446197638 1107 120-124 0.26121982482601425 1107 125-129 0.4124703849930711 1107 130-134 0.4520167630673413 1107 135-139 0.27007169976834433 1107 140-144 0.29147703116106527 1107 145-149 0.36224120155155504 1107 150-151 0.27699653705823835 1108 1 -0.07336423324052532 1108 2 7.691603270956193E-4 1108 3 0.01156350505915782 1108 4 -0.02557180858865138 1108 5 0.02980358550872353 1108 6 0.0787007078866182 1108 7 0.11758283260950009 1108 8 0.013908394753023856 1108 9 0.08360058115176372 1108 10-14 0.10744862232370878 1108 15-19 0.1109675509572483 1108 20-24 0.126651686259585 1108 25-29 0.1357390862586385 1108 30-34 0.11336918575180732 1108 35-39 0.08382092195746083 1108 40-44 0.13041418346995926 1108 45-49 0.17138703015686474 1108 50-54 0.021459182651241804 1108 55-59 0.14489865174144967 1108 60-64 0.16626781967273274 1108 65-69 0.25990594013924806 1108 70-74 0.2523095866252163 1108 75-79 0.2048623862232546 1108 80-84 0.12904607388595934 1108 85-89 0.16303879838703494 1108 90-94 0.2639375584548631 1108 95-99 0.3196945744072437 1108 100-104 0.2558211705475699 1108 105-109 0.38369184088316644 1108 110-114 0.2164452187441981 1108 115-119 0.3824194814880073 1108 120-124 0.39905747822978554 1108 125-129 0.2810781666231321 1108 130-134 0.4050487370918887 1108 135-139 0.34479471996536404 1108 140-144 0.35835810540024227 1108 145-149 0.4533789485109949 1108 150-151 0.9277983641453531 1109 1 0.0038470957355087876 1109 2 -0.016210896652957274 1109 3 -0.045791949943357224 1109 4 -0.007807437883109003 1109 5 0.012763196703623692 1109 6 -0.032591398699608476 1109 7 -0.015576208784835899 1109 8 -0.05312026286387095 1109 9 0.07452399325164549 1109 10-14 0.10125857707483732 1109 15-19 0.06434445727532534 1109 20-24 0.06549532627970223 1109 25-29 0.04959060293368367 1109 30-34 0.07812065215033925 1109 35-39 0.1954133469616437 1109 40-44 0.15152227798981954 1109 45-49 0.1382460883100407 1109 50-54 0.10705931672196556 1109 55-59 0.14375770989462922 1109 60-64 0.2248862208793767 1109 65-69 0.30501671607355973 1109 70-74 0.18788726384995158 1109 75-79 0.1804641961780078 1109 80-84 0.12323947090289522 1109 85-89 0.1637614395802629 1109 90-94 0.16488744952239642 1109 95-99 0.30822750552840006 1109 100-104 0.21427935724104685 1109 105-109 0.024836804851659622 1109 110-114 0.2143631004267874 1109 115-119 0.1885264028890461 1109 120-124 0.11491201149608088 1109 125-129 0.15462534275854978 1109 130-134 0.27391282289715235 1109 135-139 0.1608858880565336 1109 140-144 0.3561472791894076 1109 145-149 0.3079577988545523 1109 150-151 0.7280430432135603 1110 1 0.042541450877862985 1110 2 0.017573143039498973 1110 3 0.017970857758975 1110 4 0.008948679403033566 1110 5 0.021260448575631585 1110 6 -0.2423759374440877 1110 7 0.016717358568250518 1110 8 0.07137762173480411 1110 9 0.02698260423836274 1110 10-14 0.09206309795148115 1110 15-19 0.09528835625294363 1110 20-24 0.06341846781219118 1110 25-29 0.09357179045470332 1110 30-34 0.14172215910921437 1110 35-39 0.13168262117513763 1110 40-44 0.24188767764360364 1110 45-49 0.1541965930432312 1110 50-54 0.023883385288144154 1110 55-59 0.14927000684841119 1110 60-64 0.06206693340980962 1110 65-69 0.143089484096798 1110 70-74 0.2110766964233406 1110 75-79 0.12464968312133351 1110 80-84 0.19365704822810414 1110 85-89 0.22323596453397698 1110 90-94 0.2510055599866803 1110 95-99 0.14977893350783944 1110 100-104 0.2239507181158089 1110 105-109 0.34827108482429736 1110 110-114 0.38699678064583054 1110 115-119 0.31588848963944116 1110 120-124 0.31215162998862667 1110 125-129 0.4585863454075323 1110 130-134 0.3873094734560283 1110 135-139 0.34906147416416644 1110 140-144 0.47713353075173615 1110 145-149 0.508527698442613 1110 150-151 0.702524552667704 1111 1 -0.05180835421739971 1111 2 0.008422403089511477 1111 3 0.05672604751427457 1111 4 0.048987466843982475 1111 5 0.0011249505418788885 1111 6 0.04927809750331136 1111 7 0.16748740967565112 1111 8 0.0625837950441479 1111 9 0.07198162595202007 1111 10-14 0.017352552454710235 1111 15-19 0.07706814937225204 1111 20-24 0.004885553314416313 1111 25-29 0.005777578899746061 1111 30-34 0.05189870216778303 1111 35-39 0.06375428763754343 1111 40-44 -0.03144122652518888 1111 45-49 -0.063296456823565 1111 50-54 -0.08793366871540798 1111 55-59 -0.2406087071458174 1111 60-64 -0.03382533898810891 1111 65-69 0.041407476610217486 1111 70-74 0.1572761077314695 1111 75-79 0.04587548665993779 1111 80-84 0.04003556118986751 1111 85-89 0.04036178075805452 1111 90-94 0.15137601874921813 1111 95-99 -0.010141427533561398 1111 100-104 -0.14076948123959454 1111 105-109 0.0860512133560789 1111 110-114 0.1552813824187922 1111 115-119 0.14071804205730842 1111 120-124 0.25289800871659907 1111 125-129 0.19321531687382532 1111 130-134 0.13800814191156263 1111 135-139 0.0436761691000882 1111 140-144 0.11624133360660949 1111 145-149 -0.08983179061240065 1111 150-151 -0.1960185532086811 1112 1 -0.01697004169214722 1112 2 0.03179542888640441 1112 3 0.04535694607726981 1112 4 0.030391643850570915 1112 5 0.010198429692110267 1112 6 -0.0051563650453445575 1112 7 0.019406947473264324 1112 8 0.0340552376311436 1112 9 0.01900276747905849 1112 10-14 0.06890530367466852 1112 15-19 0.04423562078919474 1112 20-24 0.08755232257872336 1112 25-29 -0.0020608811096209934 1112 30-34 0.0640077891128712 1112 35-39 0.041540615218998767 1112 40-44 0.12806861323011987 1112 45-49 -0.0011735833200532397 1112 50-54 0.1120958281072717 1112 55-59 0.22460235544515683 1112 60-64 0.3101268505097394 1112 65-69 0.0965370381976669 1112 70-74 0.21421267032565794 1112 75-79 0.133625969189481 1112 80-84 0.0474379812117931 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0.14844565835294787 2118 115-119 0.17443838466417816 2118 120-124 -0.012934169204001478 2118 125-129 -0.22083061456160635 2118 130-134 -0.32966233005834766 2118 135-139 -0.4954787166760326 2118 140-144 -0.3652405318126384 2118 145-149 -0.2526564399932312 2118 150-151 -1.6040930524938375 2119 1 0.04809722822093221 2119 2 0.03576415532209154 2119 3 0.0513232648189188 2119 4 -0.09414037715722401 2119 5 -0.04222844652331048 2119 6 -0.01565364646773304 2119 7 0.08272797775464369 2119 8 -0.10483034398571789 2119 9 -0.05005307250188906 2119 10-14 -0.10085167797659267 2119 15-19 -0.09433775434805369 2119 20-24 -0.12193690232899002 2119 25-29 -0.11356621904667463 2119 30-34 -0.1771373207547029 2119 35-39 -0.10958848745195127 2119 40-44 -0.13610833905256214 2119 45-49 0.03921085398067703 2119 50-54 -0.15074101754895963 2119 55-59 -0.1802024493596477 2119 60-64 -0.07780425239933209 2119 65-69 -0.5389208866875705 2119 70-74 -0.2128315545159225 2119 75-79 0.025535058895926 2119 80-84 0.22298401182391103 2119 85-89 0.0896293889776274 2119 90-94 0.15642604694335205 2119 95-99 0.24409097080363296 2119 100-104 0.23043578516217877 2119 105-109 0.3382103594016854 2119 110-114 0.23853931283829866 2119 115-119 0.24606312513164852 2119 120-124 0.12256898974129626 2119 125-129 0.017746835291802654 2119 130-134 0.09400169004484127 2119 135-139 -0.09799607082542394 2119 140-144 -0.10853278549065237 2119 145-149 -0.1289193497872958 2119 150-151 -1.01657601022902 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 1.0 16 2.0 17 15.0 18 39.0 19 83.0 20 141.0 21 295.0 22 451.0 23 559.0 24 896.0 25 2514.0 26 1932.0 27 1173.0 28 1310.0 29 1758.0 30 2095.0 31 2745.0 32 3664.0 33 5190.0 34 7513.0 35 12123.0 36 25758.0 37 183183.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.77046203643397 9.387569818619616 7.300609864408787 53.54135828053763 2 26.051428143039207 15.489995699196262 41.27745708074069 17.181119077023844 3 23.175413607111714 19.02138959363323 24.325188110842365 33.478008688412686 4 28.985049774898297 29.009907631361937 16.712370926566734 25.29267166717303 5 31.455052655253095 28.069254777245987 19.02849183833713 21.44720072916379 6 22.430861620653328 31.326028543132328 21.48034453778197 24.762765298432377 7 17.911072004924222 14.804629085270339 38.30714051791146 28.97715839189397 8 19.673612398940975 17.028420815890087 24.84957051147999 38.44839627368894 9 26.599090123539597 15.83090344498325 26.73008708141145 30.8399193500657 10-14 26.35840294190758 23.162945221964875 22.21463772633473 28.26401410979281 15-19 27.656320529291257 21.261927834823755 21.821506245245438 29.26024539063955 20-24 28.550358545552683 22.27654132763259 21.118873613777744 28.05422651303698 25-29 29.052195796778808 21.049960897148058 21.2229446538786 28.674898652194535 30-34 28.402232379605547 21.829465390540165 21.279183186444104 28.489119043410188 35-39 29.055024527599073 21.226553572443226 20.789113155253073 28.929308744704628 40-44 28.753043306698235 21.600789188122718 21.258833974548683 28.387333530630364 45-49 29.95538116981197 21.101928852931476 21.030668146069456 27.912021831187094 50-54 29.221024186432683 20.678350277884324 20.666985511501302 29.433640024181695 55-59 29.262368048891357 20.137459593918887 21.383182231425828 29.216990125763925 60-64 28.64940288094554 20.65293224442894 21.166315729659598 29.531349144965922 65-69 29.122513485677757 22.049349491154896 20.205984602453665 28.622152420713686 70-74 28.16743125963304 22.49529051244433 20.12527592033291 29.212002307589714 75-79 28.700048457948128 21.789187405984478 20.404347640792437 29.106416495274956 80-84 28.69709264563013 20.7836495548486 21.204611801622132 29.314645997899134 85-89 29.693778665833324 21.186516917773844 20.55464540383905 28.565059012553778 90-94 29.17923416022311 20.523644249572666 20.981124690054436 29.31599690014978 95-99 29.12785567599393 20.762439642694222 20.029786004839632 30.07991867647221 100-104 28.444552833208636 20.81537815577044 20.497393934228015 30.242675076792914 105-109 28.8594205906796 20.747737459245855 20.851364075106805 29.541477874967743 110-114 29.278452709896523 21.84088754823246 20.160200025097847 28.72045971677317 115-119 29.099081323399417 22.908826872079807 19.37065917413815 28.621432630382625 120-124 29.356428298593023 23.174200309995296 19.514787912077505 27.954583479334172 125-129 29.693495055990027 23.28466922876601 18.93220432610737 28.089631389136592 130-134 28.032330336363977 23.539164862776786 19.773763404881276 28.65474139597796 135-139 28.415244913968767 24.301672009584106 19.010131813008936 28.27295126343819 140-144 28.314864800871337 24.32700352007072 19.06315606699184 28.29497561206611 145-149 27.553699152147733 24.471611708601493 19.330116198856164 28.644572940394614 150-151 27.726722093541728 25.11571379529411 18.460345582751643 28.69721852841252 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 1.5 3 2.0 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 1.5 10 1.5 11 0.5 12 1.0 13 0.5 14 1.0 15 2.5 16 5.0 17 5.5 18 4.0 19 9.0 20 17.0 21 22.5 22 36.0 23 52.0 24 65.5 25 81.5 26 96.0 27 124.0 28 164.5 29 211.0 30 238.5 31 313.0 32 399.0 33 451.0 34 614.0 35 836.5 36 1007.0 37 1281.5 38 1727.5 39 2096.0 40 2559.0 41 3028.5 42 3346.5 43 3992.5 44 5206.5 45 5705.5 46 7117.5 47 8778.5 48 9708.5 49 9720.5 50 8390.0 51 7390.0 52 7562.0 53 8333.5 54 8745.5 55 9343.5 56 9327.5 57 8067.5 58 7907.0 59 9447.5 60 8441.0 61 6284.0 62 6211.5 63 5487.0 64 4675.5 65 4949.5 66 5368.5 67 5086.5 68 4363.5 69 4174.0 70 3316.5 71 2717.0 72 2681.5 73 2828.5 74 4314.0 75 4061.0 76 2324.5 77 2570.5 78 2922.5 79 2774.5 80 1936.0 81 989.5 82 939.5 83 1015.5 84 1098.5 85 1004.5 86 716.5 87 794.0 88 920.0 89 1067.5 90 1112.5 91 1000.5 92 865.5 93 514.5 94 223.5 95 85.0 96 30.0 97 13.0 98 9.5 99 6.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04182432992294065 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 7.891383004328424E-5 20-24 1.5782766008656847E-4 25-29 0.002683070221471664 30-34 0.0029198117116015164 35-39 0.004813743632640338 40-44 0.006313106403462739 45-49 0.0021306734111686742 50-54 0.010337711735670236 55-59 0.005445054272986612 60-64 0.009548573435237392 65-69 0.009469659605194108 70-74 0.0064709340635493075 75-79 0.010022056415497098 80-84 0.008522693644674697 85-89 0.001341535110735832 90-94 0.003708950012034359 95-99 0.013178609617228469 100-104 0.012705126636968763 105-109 0.012310557486752341 110-114 0.012941868127098615 115-119 0.013020781957141899 120-124 0.0070233308738522965 125-129 0.0011837074506492636 130-134 0.0028408978815582324 135-139 0.008443779814631413 140-144 0.014599058558007583 145-149 0.018860405380344933 150-151 0.005523968103029897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 253441.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.28953484242881 #Duplication Level Percentage of deduplicated Percentage of total 1 71.30605577379332 14.467667030985515 2 9.033098673719419 3.6655474055105524 3 4.078021080471394 2.4822345240115053 4 2.401695772237564 1.9491716020691205 5 1.7346660962234064 1.7597784099652385 6 1.2387693983120065 1.5080432921271618 7 0.9509548442300961 1.3506102011908097 8 0.8226051106530279 1.3352220043323693 9 0.6845319124110303 1.2499950678856222 >10 6.059663179184007 25.5298077264531 >50 1.0092956322196724 14.408087089302835 >100 0.6456380537513127 23.847364869930278 >500 0.027225701061802342 3.589790128669 >1k 0.007778771731943526 2.8566806475668893 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC 3784 1.4930496644189377 TruSeq Adapter, Index 1 (100% over 50bp) CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA 1288 0.5082050654787504 No Hit CTCTTATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG 1160 0.4577002142510485 No Hit CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG 1008 0.39772570341815255 No Hit CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA 976 0.38509949061122706 No Hit CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCACTCCGC 919 0.3626090490488911 No Hit CTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGAT 717 0.282906080705174 No Hit GTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGG 700 0.27619840515149485 No Hit CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG 640 0.25252425613850954 No Hit CTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGT 625 0.24660571888526325 No Hit CTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAA 621 0.24502744228439757 No Hit CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCA 614 0.2422654582328826 No Hit CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC 563 0.22214243157184516 No Hit CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCT 562 0.2217478624216287 No Hit CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG 560 0.22095872412119585 No Hit GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG 539 0.21267277196665102 No Hit CTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGA 534 0.2106999262155689 No Hit CGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCT 528 0.20833251131427039 No Hit CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT 497 0.19610086765756135 No Hit CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA 497 0.19610086765756135 No Hit CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA 483 0.19057689955453144 No Hit CTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTC 480 0.18939319210388217 No Hit CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG 477 0.1882094846532329 No Hit CGCTGACCCCCCTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCA 472 0.1862366389021508 No Hit CGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCAATGAAGGTGAAG 466 0.18386922400085226 No Hit CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC 459 0.18110723994933733 No Hit GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA 425 0.167691888841979 No Hit ATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGAC 416 0.1641407664900312 No Hit CGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAG 406 0.160195074987867 No Hit GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC 400 0.15782766008656846 No Hit CCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGG 400 0.15782766008656846 No Hit GTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGAC 394 0.15546024518526996 No Hit CGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGA 390 0.15388196858440426 No Hit GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC 387 0.15269826113375498 No Hit CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC 382 0.1507254153826729 No Hit CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG 381 0.1503308462324565 No Hit CTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT 375 0.14796343133115794 No Hit CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC 370 0.14599058558007585 No Hit CTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGC 362 0.14283403237834447 No Hit GTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGCCGG 358 0.1412557557774788 No Hit CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT 356 0.14046661747704595 No Hit CTAATACATGCCGACGGGCGCTGACCCCCCTCGCGGGGGGGATGCGTGCA 355 0.1400720483268295 No Hit CTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTC 346 0.13652092597488175 No Hit GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT 342 0.13494264937401604 No Hit GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG 338 0.13336437277315036 No Hit CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC 328 0.12941868127098616 No Hit CTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAAC 328 0.12941868127098616 No Hit CACGAACGTGCGTTGCGTGACGGGCGAGGGGGCGGCCCCCTTTCCGGCCG 328 0.12941868127098616 No Hit GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCA 325 0.12823497382033688 No Hit CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGTGGGTC 322 0.1270512663696876 No Hit CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGG 304 0.11994902166579205 No Hit CCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTG 303 0.11955445251557562 No Hit CTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGC 299 0.11797617591470992 No Hit CTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTGAATTG 298 0.11758160676449352 No Hit ATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGG 298 0.11758160676449352 No Hit CTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCA 297 0.1171870376142771 No Hit CGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAA 294 0.11600333016362782 No Hit CGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGC 293 0.11560876101341142 No Hit CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACC 288 0.11363591526232929 No Hit CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT 285 0.11245220781168003 No Hit CGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG 282 0.11126850036103078 No Hit CTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTGCTTGGC 275 0.10850651630951583 No Hit CTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGG 275 0.10850651630951583 No Hit CTCCCTTTCGATCGGCTGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG 273 0.10771737800908299 No Hit CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG 272 0.10732280885886655 No Hit CGCAGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTG 269 0.1061391014082173 No Hit GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCC 269 0.1061391014082173 No Hit CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA 268 0.10574453225800087 No Hit GTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGTG 265 0.10456082480735161 No Hit GTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA 263 0.10377168650691877 No Hit GTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAC 261 0.10298254820648592 No Hit CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT 259 0.10219340990605308 No Hit GACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGT 258 0.10179884075583667 No Hit CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC 258 0.10179884075583667 No Hit CTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTA 256 0.10100970245540382 No Hit CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCA 254 0.10022056415497098 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0031565532017313693 0.0 0.0 0.0 0.0 2 0.0031565532017313693 0.0 0.0 0.0 0.0 3 0.005523968103029897 0.0 0.0 0.0 0.0 4 0.006313106403462739 0.0 0.0 0.0 0.0 5 0.006313106403462739 0.0 0.0 0.0 0.0 6 0.006313106403462739 0.0 0.0 0.0 0.0 7 0.006313106403462739 0.0 0.0 0.0 0.0 8 0.006313106403462739 0.0 0.0 0.0 0.0 9 0.007102244703895581 0.0 0.0 0.0 0.0 10-14 0.01096902237601651 0.0 0.0 0.0 0.0 15-19 0.014046661747704595 0.0 0.0 0.0 0.0 20-24 0.01791343941982552 0.0 0.0 0.0 0.0 25-29 0.024857856463634538 0.0 0.0 0.0 0.0 30-34 0.031170962867097272 0.0 0.0 0.0 0.0 35-39 0.034485343728915215 0.0 0.0 0.0 0.0 40-44 0.03961474268172869 0.0 0.0 0.0 0.0 45-49 0.04766395334614368 0.0 0.0 0.0 0.0 50-54 0.05942211402259303 0.0 0.0 0.0 0.0 55-59 0.06904960128787371 0.0 0.0 0.0 0.0 60-64 0.08925154177895447 0.0 0.0 0.0 0.0 65-69 0.11458288122284872 0.0 0.0 0.0 0.0 70-74 0.14812125899124448 0.0 0.0 0.0 0.0 75-79 0.19980981766959566 0.0 0.0 0.0 0.0 80-84 0.29229682648032485 0.0 0.0 0.0 0.0 85-89 0.4409704822818723 0.0 0.0 0.0 0.0 90-94 0.6833937681748414 0.0 0.0 0.0 0.0 95-99 1.0997431354832092 0.0 0.0 0.0 0.0 100-104 1.7275815673075783 0.0 0.0 0.0 0.0 105-109 2.616940431895392 0.0 0.0 0.0 0.0 110-114 3.875694934915819 0.0 0.0 0.0 0.0 115-119 5.558216705268682 0.0 0.0 0.0 0.0 120-124 7.708460746288091 0.0 0.0 0.0 0.0 125-129 10.642476947297398 0.0 0.0 0.0 0.0 130-134 14.109634984079136 0.0 0.0 0.0 0.0 135-139 17.80722140458726 0.0 0.0 0.0 0.0 140 19.977825213757836 0.0 0.0 0.0 0.0 >>END_MODULE