##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename DRR051431_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 327386 Sequences flagged as poor quality 0 Sequence length 151 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52164417537708 33.0 32.0 34.0 32.0 34.0 2 32.183486770967605 33.0 32.0 34.0 32.0 34.0 3 32.15377871992083 33.0 32.0 34.0 31.0 34.0 4 32.14132858460655 33.0 32.0 34.0 31.0 34.0 5 32.13380230064816 33.0 32.0 34.0 31.0 34.0 6 34.565974110071906 37.0 33.0 37.0 32.0 37.0 7 34.370437343075146 37.0 33.0 37.0 32.0 37.0 8 34.44808880037631 37.0 33.0 37.0 32.0 37.0 9 34.38052024216063 37.0 34.0 37.0 32.0 37.0 10-14 35.193727282168446 37.4 35.6 37.4 32.8 37.4 15-19 36.26708289297649 38.0 38.0 38.0 34.0 38.0 20-24 36.59018467497083 38.0 38.0 38.4 34.4 38.4 25-29 36.63514933442481 38.0 38.0 39.0 34.0 39.0 30-34 36.54597997470876 38.0 38.0 39.0 33.8 39.0 35-39 36.52441460538935 38.0 38.0 39.0 33.0 39.0 40-44 36.544775280555676 38.0 38.0 39.0 33.0 39.0 45-49 36.192216527279726 38.0 38.0 39.0 31.8 39.0 50-54 36.09835179268509 38.0 38.0 39.0 31.6 39.0 55-59 36.13566737734662 38.0 38.0 39.0 31.0 39.0 60-64 36.49100266963156 38.0 37.0 39.0 32.2 39.0 65-69 36.27426646221891 38.0 37.0 39.0 30.8 39.0 70-74 35.79502788757003 38.0 37.0 39.0 28.4 39.0 75-79 35.681027288888345 38.0 37.0 39.0 27.0 39.0 80-84 35.58864887319556 38.0 37.0 39.0 26.0 39.0 85-89 35.50856420250103 38.0 37.0 39.0 26.0 39.0 90-94 35.39930601797267 38.0 37.0 39.0 25.0 39.0 95-99 35.27863622757235 38.0 37.0 39.0 24.2 39.0 100-104 35.18579719352691 38.0 37.0 39.0 24.0 39.0 105-109 35.0599072654298 38.0 37.0 39.0 23.0 39.0 110-114 34.883505708857435 38.0 37.0 39.0 20.0 39.0 115-119 34.743755689003194 38.0 36.8 39.0 17.2 39.0 120-124 34.59244928005474 38.0 36.0 39.0 16.0 39.0 125-129 34.41763789532845 38.0 35.6 39.0 15.2 39.0 130-134 34.27746696560024 38.0 35.0 39.0 14.8 39.0 135-139 34.096274122900795 38.0 35.0 39.0 14.0 39.0 140-144 33.92466446335518 38.0 34.2 39.0 14.0 39.0 145-149 33.638939355989564 38.0 33.8 39.0 14.0 39.0 150-151 31.9946973908475 37.0 29.0 38.5 13.5 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.28680657172556323 1101 2 -0.2165470414607178 1101 3 -0.2829070143867405 1101 4 -0.3290497590047323 1101 5 -0.3445630259385055 1101 6 -0.2939904620779856 1101 7 -0.29689769664506116 1101 8 0.024385353329691384 1101 9 -0.07927183480355637 1101 10-14 -0.21996108440313122 1101 15-19 -0.21512317056599528 1101 20-24 -0.1727030508290639 1101 25-29 -0.2829802547054143 1101 30-34 -0.3089268281381692 1101 35-39 -0.28093013978344317 1101 40-44 -0.39353861328086026 1101 45-49 -0.5457020175379981 1101 50-54 -0.4095602322198033 1101 55-59 -0.4988274022839363 1101 60-64 -0.3841137143014137 1101 65-69 -0.3155264895275778 1101 70-74 -0.4300890747069275 1101 75-79 -0.5181973254654082 1101 80-84 -0.5249988597377566 1101 85-89 -0.4191355054621795 1101 90-94 -0.386959223806997 1101 95-99 -0.519609099029978 1101 100-104 -0.43880344905972635 1101 105-109 -0.294373614211473 1101 110-114 -0.5270156389040395 1101 115-119 -0.39876598309734135 1101 120-124 -0.49216850527550804 1101 125-129 -0.3831360620517117 1101 130-134 -0.4719801515710671 1101 135-139 -0.43910433164992213 1101 140-144 -0.4969097736964656 1101 145-149 -0.5611009063395827 1101 150-151 -0.5304125104725372 1102 1 0.0677635361094957 1102 2 -2.540550651630724E-4 1102 3 -0.03781036904636892 1102 4 -0.017955949647493696 1102 5 -0.04239541707850236 1102 6 0.05657391361079789 1102 7 0.21368082134688393 1102 8 0.11902218575468027 1102 9 0.07441814033457206 1102 10-14 0.10381430874160458 1102 15-19 0.1748243599847683 1102 20-24 0.1258707580768217 1102 25-29 0.23694993647896467 1102 30-34 0.22564230177167133 1102 35-39 0.2197477128432297 1102 40-44 0.13303770814059135 1102 45-49 0.18951179076609748 1102 50-54 0.14647751569364686 1102 55-59 0.11294245429956362 1102 60-64 0.1805923627515611 1102 65-69 0.11057568861278355 1102 70-74 0.16134956612747686 1102 75-79 0.31280185082065515 1102 80-84 0.153446088714297 1102 85-89 0.15810501524539688 1102 90-94 0.33252337852723457 1102 95-99 0.3846642085011851 1102 100-104 0.23448301908811686 1102 105-109 0.22975593831250052 1102 110-114 0.2171847003607894 1102 115-119 0.36375194866604943 1102 120-124 0.29253584160208845 1102 125-129 0.43558563923726723 1102 130-134 0.41143461284298866 1102 135-139 0.3210450652335126 1102 140-144 0.38253672062968747 1102 145-149 0.34214723466219255 1102 150-151 0.17954088762953546 1103 1 -0.15210922401698923 1103 2 -0.29760008728922216 1103 3 -0.023497765522805025 1103 4 -0.15247635320117325 1103 5 -0.22058770739622702 1103 6 0.05021191993514407 1103 7 -0.09422461458144227 1103 8 -0.07608020846306118 1103 9 0.1296169055807681 1103 10-14 0.10037958505541411 1103 15-19 0.08373943301592846 1103 20-24 -0.0457705610016248 1103 25-29 0.045118886938233516 1103 30-34 0.12372843337835349 1103 35-39 0.020862755758450646 1103 40-44 0.08782614361717833 1103 45-49 0.03021499811438133 1103 50-54 0.0370918810000731 1103 55-59 -0.049987074385882124 1103 60-64 0.10996971555988466 1103 65-69 -0.02191188855933035 1103 70-74 0.1276693974781722 1103 75-79 0.28661377673542177 1103 80-84 0.14938796493775186 1103 85-89 0.1320035245060467 1103 90-94 0.11911877078743771 1103 95-99 0.1709185376684772 1103 100-104 0.29068539824788076 1103 105-109 0.25338114535963996 1103 110-114 0.22933723762927372 1103 115-119 0.23087211129215746 1103 120-124 0.18397839543434458 1103 125-129 0.2262301204900936 1103 130-134 0.1835645631516769 1103 135-139 0.17580790215566822 1103 140-144 0.22864875195027423 1103 145-149 0.4248591756349356 1103 150-151 0.298892265434084 1104 1 -0.17944764172724348 1104 2 -0.18554744321539118 1104 3 -0.14772175507707885 1104 4 -0.1903450694454598 1104 5 -0.09827332672081468 1104 6 0.04054072283145871 1104 7 -0.12744913829204307 1104 8 0.08164873687494634 1104 9 0.06073596309160223 1104 10-14 0.04785718016490392 1104 15-19 -0.03670718102645054 1104 20-24 -0.10853927131085328 1104 25-29 -0.005179053592371474 1104 30-34 0.0018476095905910483 1104 35-39 -0.003546111236623517 1104 40-44 0.06998710272453934 1104 45-49 -0.04334723109803207 1104 50-54 -0.1614676774306929 1104 55-59 -0.10197265216496731 1104 60-64 -0.04533297302697292 1104 65-69 -0.06268048016713834 1104 70-74 -0.023548478953664187 1104 75-79 -0.055186033342323526 1104 80-84 -0.12335403755496799 1104 85-89 -0.06794899493130657 1104 90-94 -0.04535033717149872 1104 95-99 -0.07693445063943471 1104 100-104 -0.05688181642361201 1104 105-109 -0.16975702307765772 1104 110-114 -0.009605429591331927 1104 115-119 -0.1870449757753221 1104 120-124 -0.05975060925435827 1104 125-129 -0.09179765563904851 1104 130-134 -0.03964957418585158 1104 135-139 -0.06827661324584255 1104 140-144 -0.01416269086532651 1104 145-149 0.03467044039842904 1104 150-151 0.20883573388918109 1105 1 0.123420129692974 1105 2 0.1284900224694283 1105 3 -0.059734569063792264 1105 4 0.1146633423456791 1105 5 -0.034510158573063165 1105 6 -0.10821586963928098 1105 7 0.08368010175076535 1105 8 0.14552983430393596 1105 9 0.0234927018543587 1105 10-14 0.11096815460411591 1105 15-19 -0.038154427079170716 1105 20-24 0.08309357154615071 1105 25-29 0.09963902929549562 1105 30-34 0.011837115377332452 1105 35-39 -0.037705447130562675 1105 40-44 0.015364200261004157 1105 45-49 0.17928489074829912 1105 50-54 -0.03355710066693973 1105 55-59 -0.05091912570627244 1105 60-64 0.054302789699306686 1105 65-69 -0.20629535597566218 1105 70-74 0.029580155022209453 1105 75-79 0.09970989779891681 1105 80-84 0.09851348457169706 1105 85-89 0.08231306184492126 1105 90-94 0.08398942305887402 1105 95-99 0.15853181039031483 1105 100-104 0.057516154595646185 1105 105-109 0.16647995805215743 1105 110-114 0.027498406394414587 1105 115-119 0.0418597896125803 1105 120-124 -0.00383959062899919 1105 125-129 0.04975047219629403 1105 130-134 -0.034985396473885544 1105 135-139 -0.15533544204387795 1105 140-144 0.03664019855134626 1105 145-149 0.09606263288290506 1105 150-151 0.07145073359809118 1106 1 -0.10900577574113157 1106 2 -0.1657035355650862 1106 3 -0.3286701793159068 1106 4 -0.24213152561496543 1106 5 -0.33696701774105264 1106 6 -0.03082256282347373 1106 7 -0.26750047665040455 1106 8 -1.1165974498423665 1106 9 -0.5859003357985415 1106 10-14 -0.5330686908066511 1106 15-19 -0.4605709118116508 1106 20-24 -0.09870159548439261 1106 25-29 -0.12953628547556661 1106 30-34 -0.2583405321388952 1106 35-39 -0.17293034768982096 1106 40-44 -0.08924282190006494 1106 45-49 -0.3049574156787216 1106 50-54 -0.2842874293447579 1106 55-59 -0.1999870743858736 1106 60-64 -0.12778890512977625 1106 65-69 -0.14794637131794985 1106 70-74 -0.3014685335563101 1106 75-79 -0.23924829223009425 1106 80-84 -0.23578444885534822 1106 85-89 -0.3409275099767157 1106 90-94 -0.2776053671436003 1106 95-99 -0.3763228416418727 1106 100-104 -0.48034908451073477 1106 105-109 -0.44954988912311933 1106 110-114 -0.46049034857762194 1106 115-119 -0.3515416818112911 1106 120-124 -0.2525733287035905 1106 125-129 -0.5201491898547275 1106 130-134 -0.3274699196069477 1106 135-139 -0.3102265806029578 1106 140-144 -0.12083400667042099 1106 145-149 -0.09876151402023226 1106 150-151 0.0053577826754604985 1107 1 -0.11702730204621759 1107 2 0.0012792956814635659 1107 3 -0.039253219724933786 1107 4 -0.07585171269918334 1107 5 -0.07084407668420312 1107 6 -0.031264128056022855 1107 7 -0.014353598109892118 1107 8 -0.44735740684785696 1107 9 -0.4651896191265479 1107 10-14 -0.33207110198522827 1107 15-19 -0.11141308129136007 1107 20-24 -0.015570694633211701 1107 25-29 -0.09786661693998866 1107 30-34 -0.09804450913101448 1107 35-39 -0.0491472666103121 1107 40-44 -0.024171358053216352 1107 45-49 -0.14322281962375882 1107 50-54 -0.07040920838783649 1107 55-59 -0.12092031669480008 1107 60-64 -0.11196227758423305 1107 65-69 -0.1126759126130139 1107 70-74 -0.09192186417828196 1107 75-79 -0.18618624069478074 1107 80-84 -0.15609122049058755 1107 85-89 -0.01083992318881144 1107 90-94 -0.207778361943582 1107 95-99 -0.20967946366783252 1107 100-104 -0.13651502007707705 1107 105-109 -0.11449454821734406 1107 110-114 -0.24597331055072402 1107 115-119 -0.14390913961566554 1107 120-124 -0.06402235987460614 1107 125-129 -0.12288674904480246 1107 130-134 -0.03864282725589163 1107 135-139 -0.09028627906038622 1107 140-144 -0.0704945533978929 1107 145-149 -0.057272829129317415 1107 150-151 -0.01131566240486137 1108 1 -0.1780969805675312 1108 2 -0.23446499531548426 1108 3 -0.2310833809483661 1108 4 -0.3205812801545065 1108 5 -0.3497575778640787 1108 6 -0.12364210502492767 1108 7 -0.2012749487625456 1108 8 -0.3412233444422412 1108 9 1.2303936479440836E-5 1108 10-14 -0.09129546078359141 1108 15-19 -0.05148946625655526 1108 20-24 -0.12639811172027038 1108 25-29 -0.1335591754028158 1108 30-34 -0.166045624704104 1108 35-39 -0.17887078656129773 1108 40-44 -0.14646793575823125 1108 45-49 -0.22676435954812035 1108 50-54 -0.261689097292944 1108 55-59 -0.2467467005762103 1108 60-64 -0.06909132424610931 1108 65-69 -0.3014310226458079 1108 70-74 -0.17228387631374176 1108 75-79 -0.3206882283512158 1108 80-84 -0.3017086222746457 1108 85-89 -0.19169671799706123 1108 90-94 -0.12817051767296306 1108 95-99 -0.18226801228124856 1108 100-104 -0.01464848712504363 1108 105-109 -0.23037499115186932 1108 110-114 -0.12911399558773695 1108 115-119 -0.0679777733709983 1108 120-124 -0.05018910914955654 1108 125-129 -0.1403606407330642 1108 130-134 -0.05565025911129595 1108 135-139 -0.06851338488520753 1108 140-144 -0.1634959218534746 1108 145-149 0.034981018658534424 1108 150-151 0.03608174316519808 1109 1 -0.0706738308421393 1109 2 -0.19415106762304646 1109 3 -0.19677288378765923 1109 4 -0.13176735912185222 1109 5 -0.06620000694101691 1109 6 -0.22707974053315638 1109 7 -0.10949342538742712 1109 8 -0.13271568494397457 1109 9 -0.28581033550101864 1109 10-14 -0.0275621602065641 1109 15-19 0.07819972875245185 1109 20-24 -0.025262691554125638 1109 25-29 0.1042414956080151 1109 30-34 -0.03907332794977947 1109 35-39 -0.13273383464350275 1109 40-44 -0.10487059257038567 1109 45-49 -0.06604069198706952 1109 50-54 -0.06457929807820051 1109 55-59 0.04621967340502664 1109 60-64 -0.05100451906568537 1109 65-69 -0.03771296558768 1109 70-74 0.0047682639207096145 1109 75-79 -0.07491712088737756 1109 80-84 0.04253990924170381 1109 85-89 -0.03307636005555992 1109 90-94 0.013473000057615536 1109 95-99 -0.07713790218752337 1109 100-104 -0.051043202507827345 1109 105-109 -0.14097323267136375 1109 110-114 -0.24665440201250988 1109 115-119 -0.37533166128229567 1109 120-124 -0.12938954958366367 1109 125-129 -0.5077870167566374 1109 130-134 -0.46001834125461727 1109 135-139 -0.17767518373884172 1109 140-144 -0.019864234869523045 1109 145-149 -0.05253542732242522 1109 150-151 -0.25130372694936653 1110 1 0.046819273667445316 1110 2 0.13603830095890856 1110 3 0.1394045087463951 1110 4 0.06906725908164901 1110 5 -0.040449215906299685 1110 6 0.012084726047056904 1110 7 0.1746111129782193 1110 8 0.2812890431814381 1110 9 0.13844629114461782 1110 10-14 0.05517885351357421 1110 15-19 0.08269978816770163 1110 20-24 -0.0013886843608190702 1110 25-29 0.00995384755886164 1110 30-34 0.09959256717971954 1110 35-39 0.11801077499092116 1110 40-44 0.12702174256705945 1110 45-49 -0.2537515515449158 1110 50-54 0.09024164059418638 1110 55-59 0.040917471202753575 1110 60-64 -0.0671712242354019 1110 65-69 0.11189566873986934 1110 70-74 0.17624230093235127 1110 75-79 0.04155714123795207 1110 80-84 0.18577125746600132 1110 85-89 0.11553498375503324 1110 90-94 0.049307850755582194 1110 95-99 0.09296877217483512 1110 100-104 0.11290185203981196 1110 105-109 0.09453642101522064 1110 110-114 0.2187887287357313 1110 115-119 0.06655350712118491 1110 120-124 0.07836764648888561 1110 125-129 0.07578765566605483 1110 130-134 0.19195915328175772 1110 135-139 0.2366993212878441 1110 140-144 0.21533734520710368 1110 145-149 0.16301409731866556 1110 150-151 -0.09110397361491351 1111 1 -0.21066575627267525 1111 2 -0.31391892744143135 1111 3 -0.11209249724163328 1111 4 -0.11485061944700448 1111 5 -0.15507710298927435 1111 6 -0.33864239114712547 1111 7 -0.10682601277028425 1111 8 -0.05746202610855278 1111 9 -0.05573677130191612 1111 10-14 -0.12497512424519641 1111 15-19 -0.1934336592232313 1111 20-24 -0.13380955690145413 1111 25-29 -0.22956711052497525 1111 30-34 -0.214179887910106 1111 35-39 -0.07702771944876474 1111 40-44 -0.0959038348495369 1111 45-49 -0.22979945573653282 1111 50-54 -0.10479685384550663 1111 55-59 -0.13615695138065576 1111 60-64 -0.1402251164509707 1111 65-69 -0.2662052722353252 1111 70-74 -0.25164478204455776 1111 75-79 -0.18923841051569212 1111 80-84 -0.04348451669994802 1111 85-89 -0.16761300597978135 1111 90-94 -0.24205228611354102 1111 95-99 -0.05382335785082404 1111 100-104 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-0.11979649769071443 2113 1 -0.0036198219290426437 2113 2 0.026003634202822923 2113 3 0.29610909076548353 2113 4 0.26031160227896777 2113 5 0.21270799879571456 2113 6 0.3211546595745034 2113 7 0.21950809370995472 2113 8 0.1340784007227569 2113 9 0.17275846032466546 2113 10-14 0.0732693495143053 2113 15-19 0.0665585926604706 2113 20-24 0.0399873982172565 2113 25-29 0.04361677310773615 2113 30-34 0.05367206456526219 2113 35-39 0.11220511001918965 2113 40-44 0.03902311810740855 2113 45-49 0.2417691668181874 2113 50-54 0.08049385390852848 2113 55-59 0.061571552055703194 2113 60-64 -0.121392770419817 2113 65-69 0.025481341431181193 2113 70-74 0.05849019641981812 2113 75-79 -0.08222850562630413 2113 80-84 0.04550053000635046 2113 85-89 0.021057621310852426 2113 90-94 -0.08247048462655471 2113 95-99 0.05315301467517486 2113 100-104 -0.00983630086919618 2113 105-109 -0.12809321545829278 2113 110-114 -0.09668602888592659 2113 115-119 0.03238731073997059 2113 120-124 -0.07511711519232733 2113 125-129 -0.013879453580251777 2113 130-134 0.03576711700402058 2113 135-139 0.02233748140272951 2113 140-144 -0.040379173619882636 2113 145-149 0.04997464542298502 2113 150-151 0.1303426838862407 2114 1 0.14967277089159126 2114 2 0.10783846860061885 2114 3 0.16478041082526573 2114 4 0.22415521356577983 2114 5 0.2836647804538117 2114 6 -0.07294822817871705 2114 7 0.07044791492561586 2114 8 0.09547730137262533 2114 9 -0.062190546397935975 2114 10-14 -7.08126122553665E-4 2114 15-19 -0.04624668130944798 2114 20-24 -0.03687447213331296 2114 25-29 -0.09929328616984634 2114 30-34 -0.02090938194216818 2114 35-39 0.08075398464065131 2114 40-44 0.15438365345285376 2114 45-49 0.00817727536502133 2114 50-54 0.07264615084501713 2114 55-59 -0.06182924055111272 2114 60-64 -0.06887893058684114 2114 65-69 -0.03681931739524913 2114 70-74 -0.05491680941837984 2114 75-79 0.05109190679790743 2114 80-84 0.10004290592363674 2114 85-89 0.16932127502675343 2114 90-94 0.08958046946830223 2114 95-99 -0.058513306811967425 2114 100-104 0.017388707667002734 2114 105-109 0.07604488060610493 2114 110-114 0.021265032121291938 2114 115-119 0.20309150495102557 2114 120-124 0.06568170485345348 2114 125-129 0.20846802838178746 2114 130-134 0.18239238078648157 2114 135-139 0.2752883464972484 2114 140-144 0.08288503984658746 2114 145-149 0.055740915972819494 2114 150-151 -0.04744249503803033 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 72.0 15 306.0 16 3282.0 17 6995.0 18 3266.0 19 1861.0 20 1462.0 21 1442.0 22 1495.0 23 1838.0 24 2186.0 25 2613.0 26 3216.0 27 3916.0 28 4324.0 29 4562.0 30 4675.0 31 5249.0 32 6294.0 33 7559.0 34 10161.0 35 15001.0 36 28215.0 37 76299.0 38 131096.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.76922653992535 12.358500363485305 6.999382991331333 48.872890105258016 2 27.181980903276255 17.23989419217682 34.024057229081265 21.554067675465657 3 23.716041614485654 19.71067791536596 24.195597856963953 32.37768261318444 4 27.34600746519399 26.26929679338762 20.116009847702713 26.268685893715677 5 32.67549620325853 25.226491053374307 20.445590220718053 21.652422522649108 6 27.78157893129211 29.761810217907914 20.204895749970984 22.251715100828992 7 24.995570977378385 17.3022059587154 32.85265710812313 24.84956595578308 8 21.87234640454998 22.18848698478249 26.11260102753325 29.826565583134286 9 26.418050863506686 19.30717868204505 27.37166525141576 26.903105203032506 10-14 28.451613691483445 23.252002223674808 22.216099649954486 26.080284434887258 15-19 28.678440739677324 21.763606262943437 22.89517572529064 26.662777272088604 20-24 29.73364774303116 22.255136138991894 22.11743935293507 25.893776765041878 25-29 27.513821605077798 24.893489642196062 21.747417421636843 25.845271331089297 30-34 29.164350338743866 22.664805458999467 21.915109381586262 26.2557348206704 35-39 26.753984593110275 22.372123426169722 23.349318541415943 27.524573439304064 40-44 30.32646478468841 21.264379051028452 21.814799655452585 26.59435650883055 45-49 29.175468712773302 21.463287984214354 21.76012413481334 27.601119168199006 50-54 28.338169622402916 22.444759397164205 22.135766343093476 27.08130463733941 55-59 26.976290983731744 22.436328981691336 23.314008540377415 27.273371494199505 60-64 26.637058395899643 25.26149560457686 21.13340216136304 26.96804383816046 65-69 27.249485317026384 24.72756929129529 21.37232502306146 26.650620368616863 70-74 27.326092135888523 23.69643173501616 21.07646631193759 27.90100981715773 75-79 27.78609958886452 22.699076930595687 21.80600270017655 27.70882078036324 80-84 27.904369765353437 21.591210375520028 22.390022786557765 28.11439707256877 85-89 28.039012053050527 22.252570360369717 21.759940864911755 27.948476721668 90-94 28.227657871747724 22.086100199764193 21.931664762696023 27.75457716579206 95-99 28.17562643217879 22.472874510745427 21.66221015083733 27.689288906238453 100-104 27.47454075617161 23.001350088275004 21.96330936570287 27.560799789850517 105-109 27.614253511145865 23.00287733745487 22.106809698643193 27.276059452756073 110-114 28.83024930815612 23.067571612714044 21.40005986816785 26.702119210961982 115-119 28.36416751378191 23.234131250733082 21.287313797552486 27.114387437932518 120-124 28.923490267445928 23.38012216779103 21.4315953837949 26.264792180968143 125-129 29.81039495298819 23.925961358128546 20.672613167706114 25.591030521177156 130-134 29.93672908232428 23.87690608450336 20.84586442050938 25.34050041266298 135-139 30.07723609270775 24.41071054395151 20.80035236714604 24.711700996194704 140-144 30.37154918047809 24.522612451357116 20.916105148051535 24.18973322011326 145-149 30.645617494711132 24.309178956448324 21.285773543018667 23.759430005821876 150-151 31.079978985051287 24.85109320496295 20.285839956503942 23.78308785348182 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 1.0 5 1.0 6 2.0 7 2.0 8 1.5 9 1.0 10 1.5 11 3.0 12 5.0 13 5.0 14 7.5 15 10.0 16 13.5 17 21.0 18 31.0 19 45.5 20 63.0 21 96.5 22 173.5 23 226.0 24 262.5 25 352.0 26 445.5 27 564.0 28 707.5 29 830.0 30 964.5 31 1130.0 32 1313.0 33 1533.5 34 1835.5 35 2187.0 36 2592.5 37 2995.5 38 3465.0 39 3919.0 40 4398.5 41 5255.0 42 6386.0 43 7498.5 44 8690.0 45 9825.5 46 10893.0 47 11991.5 48 12883.5 49 12081.5 50 10325.0 51 9889.0 52 9782.5 53 10176.0 54 10919.5 55 10955.0 56 11290.5 57 10444.0 58 9078.5 59 9422.0 60 9083.0 61 7895.5 62 7442.5 63 6398.5 64 5298.5 65 4910.0 66 5388.0 67 5435.5 68 4191.5 69 3729.0 70 3638.5 71 3333.0 72 2817.5 73 2710.5 74 3766.0 75 4282.5 76 3729.5 77 3054.5 78 2698.0 79 2616.5 80 1907.5 81 1139.0 82 1064.0 83 1258.0 84 1391.0 85 1232.5 86 951.5 87 923.0 88 913.5 89 909.5 90 969.0 91 1007.5 92 887.5 93 604.0 94 459.5 95 328.5 96 143.0 97 93.5 98 145.0 99 333.0 100 276.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 6.108996719468762E-5 100-104 0.0 105-109 0.0 110-114 0.0 115-119 1.2217993438937524E-4 120-124 6.108996719468762E-5 125-129 6.108996719468762E-5 130-134 6.108996719468762E-5 135-139 6.108996719468762E-5 140-144 0.0 145-149 6.108996719468762E-5 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 327386.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.68878335764435 #Duplication Level Percentage of deduplicated Percentage of total 1 82.72089119415479 32.003706385619246 2 6.085638160668195 4.708918727822101 3 3.006095491908634 3.4890653171653336 4 1.6874514325940442 2.611417716087103 5 1.071131087437982 2.0720379294763043 6 0.7119890679699473 1.6527594482220247 7 0.5161377821755484 1.3978119986099375 8 0.3969443713873198 1.2285835831712266 9 0.3235530661407577 1.126608703255921 >10 2.861471750796067 23.43956730795761 >50 0.41588406306385195 11.144266741881944 >100 0.19728845496387662 13.400842602522733 >500 0.0047349229191330396 1.340633825473374 >1k 7.891538198555064E-4 0.38377971273514716 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT 1257 0.3839504438186117 No Hit CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCACTCCGC 844 0.25779966156158174 No Hit CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA 815 0.24894161631835204 No Hit CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT 779 0.23794542222330828 No Hit CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG 737 0.22511652911242386 No Hit CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC 639 0.19518244518702693 No Hit CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG 577 0.17624455535667377 No Hit AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG 450 0.13745242618804712 No Hit CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC 432 0.13195432914052524 No Hit CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG 397 0.12126358488145492 No Hit CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA 391 0.1194308858656143 No Hit CTCTTATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG 387 0.11820908652172055 No Hit CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT 357 0.1090455914425174 No Hit CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG 349 0.10660199275472988 No Hit CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC 345 0.10538019341083614 No Hit GGAGTCTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGC 343 0.10476929373888927 No Hit CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT 343 0.10476929373888927 No Hit CTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGC 343 0.10476929373888927 No Hit AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG 338 0.10324204455902208 No Hit CTAACACGTGCGCTCATGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGG 332 0.10140934554318144 No Hit GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC 332 0.10140934554318144 No Hit GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA 330 0.10079844587123456 No Hit CTACGAATGGTTTAGCACCAGGTTCCCCACGAACGTGCGTTGCGTGACGG 329 0.10049299603526113 No Hit CTCCCTTTCGATCGGCTGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG 328 0.10018754619928769 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.004276297703628133 0.0 0.0 0.0 0.0 2 0.004276297703628133 0.0 0.0 0.0 0.0 3 0.005192647211548447 0.0 0.0 0.0 0.0 4 0.006108996719468762 0.0 0.0 0.0 0.0 5 0.006719896391415638 0.0 0.0 0.0 0.0 6 0.007330796063362514 0.0 0.0 0.0 0.0 7 0.007636245899335952 0.0 0.0 0.0 0.0 8 0.008247145571282827 0.0 0.0 0.0 0.0 9 0.009163495079203143 0.0 0.0 0.0 0.0 10-14 0.01258453324210565 0.0 0.0 0.0 0.0 15-19 0.01588339147061878 0.0 0.0 0.0 0.0 20-24 0.019793149371078785 0.0 0.0 0.0 0.0 25-29 0.02523015645140599 0.0 0.0 0.0 0.0 30-34 0.03286640235074194 0.0 0.0 0.0 0.0 35-39 0.039769568643741635 0.0 0.0 0.0 0.0 40-44 0.0503381329684226 0.0 0.0 0.0 0.0 45-49 0.06530517493112106 0.0 0.0 0.0 0.0 50-54 0.08485396443342111 0.0 0.0 0.0 0.0 55-59 0.11570439786673833 0.0 0.0 3.054498359734381E-4 0.0 60-64 0.1637822020489575 0.0 0.0 3.054498359734381E-4 0.0 65-69 0.2413053704190161 0.0 0.0 3.054498359734381E-4 0.0 70-74 0.35749848802331197 0.0 0.0 4.276297703628133E-4 0.0 75-79 0.530566365085862 0.0 0.0 6.108996719468762E-4 0.0 80-84 0.7776752823883734 0.0 0.0 6.108996719468762E-4 0.0 85-89 1.1456812447691715 0.0 0.0 6.108996719468762E-4 0.0 90-94 1.6459469861264684 0.0 0.0 6.108996719468762E-4 0.0 95-99 2.376644083742127 0.0 0.0 6.108996719468762E-4 0.0 100-104 3.396113456287074 0.0 0.0 6.108996719468762E-4 0.0 105-109 4.784321870819156 0.0 0.0 6.108996719468762E-4 0.0 110-114 6.5118239631505315 0.0 0.0 7.330796063362515E-4 0.0 115-119 8.661396638830006 0.0 0.0 9.163495079203141E-4 0.0 120-124 11.088012315737386 0.0 0.0 9.163495079203141E-4 0.0 125-129 13.851111531953105 0.0 0.0 9.163495079203141E-4 0.0 130-134 17.218757063527455 0.0 0.0 9.163495079203141E-4 0.0 135-139 20.64016176623313 0.0 0.0 9.163495079203141E-4 0.0 140 22.642996340710965 0.0 0.0 9.163495079203142E-4 0.0 >>END_MODULE