FastQCFastQC Report
Fri 12 Jan 2024
DRR051431_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR051431_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327386
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC131424.014221744362923TruSeq Adapter, Index 7 (97% over 37bp)
CTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT14020.4282406700347602No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA9000.27490485237609424No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCACTCCGC8470.25871601106950204No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT8070.24649801763056453No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG7950.24283261959888325No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC6460.197320594038841No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG5540.16921920912928468No Hit
CTCTTATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG5470.16708106027747063No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC4850.14814317044711747No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG4370.13348157832039245No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG4270.13042707996065805No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC4110.12553988258508306No Hit
CTACGAATGGTTTAGCACCAGGTTCCCCACGAACGTGCGTTGCGTGACGG4070.12431808324118931No Hit
CTCTCGCGCGCGTCACCGACTGCCAGCGACGGCCGGGTATGGGCCCGACG3940.1203472353735346No Hit
CTCCCTTTCGATCGGCTGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG3900.11912543602964085No Hit
CTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGC3870.11820908652172055No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT3760.11484913832601272No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCATGCTC3670.11210008980225177No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG3670.11210008980225177No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA3620.11057284062238458No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC3540.1081292419345971No Hit
GGAGTCTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGC3490.10660199275472988No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC3480.10629654291875645No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA3450.10538019341083614No Hit
GTCTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCA3440.1050747435748627No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA3400.10385294423096895No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT3360.1026311448870752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)