FastQCFastQC Report
Fri 12 Jan 2024
DRR051428_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR051428_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415068
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT25000.6023109466400686No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCACTCCGC16480.39704337602513323No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC12840.30934690219433925No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT12250.2951323638536336No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG9480.22839631096591403No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC8990.2165910164117687No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA8690.20936328505208784No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC8490.2045447974789673No Hit
CTACGAATGGTTTAGCACCAGGTTCCCCACGAACGTGCGTTGCGTGACGG8050.19394412481810208No Hit
CTCTTATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG7840.18888471286632552No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG7800.18792101535170141No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG7610.1833434521572369No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT6750.16262395559281853No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG6690.16117840932088237No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC6680.16093748494222634No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA6590.15876916553432208No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT6570.15828731677701005No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA6410.1544325267185136No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA6410.1544325267185136No Hit
CTCTCGCGCGCGTCACCGACTGCCAGCGACGGCCGGGTATGGGCCCGACG6310.15202328293195333No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA6210.14961403914539304No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT6140.14792756849480085No Hit
GTCTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCA6090.14672294660152072No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA6070.14624109784420866No Hit
GGAGTCTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGC6040.14551832470824058No Hit
CTCCCTTTCGATCGGCTGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG5990.14431370281496042No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG5610.13515857642603138No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG5570.13419487891140727No Hit
GTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAC5560.13395395453275125No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCATGCTC5540.13347210577543922No Hit
CTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGC5380.12961731571694277No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG5200.12528067690113426No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT5130.12359420625054209No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA5020.12094403808532578No Hit
CTAACACGTGCGCTCATGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGG4990.1202212649493577No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG4930.11877571867742152No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT4860.11708924802682934No Hit
CCCACCCCGCGGACGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGA4800.11564370175489316No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT4720.11371630672564496No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA4670.11251168483236482No Hit
CTACTCTTATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCC4630.11154798731774071No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT4620.11130706293908468No Hit
CTCCCGTCCCTCTCGCGCGCGTCACCGACTGCCAGCGACGGCCGGGTATG4600.11082521418177263No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT4590.1105842898031166No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA4550.10962059228849248No Hit
CTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTC4550.10962059228849248No Hit
CCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACC4440.10697042412327619No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT4410.1062476509873081No Hit
CTCGCGCGCGTCACCGACTGCCAGCGACGGCCGGGTATGGGCCCGACGCT4340.10456118033671591No Hit
CGACGGGCGCTGACCCCCCTCGCGGGGGGGATGCGTGCATTTATCAGATC4280.10311563406477973No Hit
CTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTA4280.10311563406477973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)