##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename DRR051428_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 415068 Sequences flagged as poor quality 0 Sequence length 151 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.760737999556696 33.0 32.0 34.0 32.0 34.0 2 32.77540547572928 34.0 33.0 34.0 32.0 34.0 3 32.878824674511165 34.0 33.0 34.0 32.0 35.0 4 32.89495938014976 34.0 33.0 34.0 32.0 35.0 5 32.95925727832548 34.0 33.0 34.0 32.0 35.0 6 34.9547182630316 37.0 34.0 37.0 33.0 37.0 7 34.985452986016746 37.0 34.0 37.0 33.0 37.0 8 34.99840989910087 37.0 34.0 37.0 33.0 37.0 9 35.01189202733046 37.0 34.0 37.0 33.0 37.0 10-14 35.795269208900706 37.4 35.6 37.4 34.6 37.4 15-19 36.97555773993659 38.0 38.0 38.0 37.0 38.0 20-24 37.2061710370349 38.4 38.0 38.4 37.0 38.4 25-29 37.40935702101824 39.0 38.0 39.0 37.0 39.0 30-34 37.32133240818372 38.8 38.0 39.0 36.6 39.0 35-39 37.26377605597155 38.6 38.0 39.0 36.0 39.0 40-44 37.240246899303244 38.2 38.0 39.0 36.0 39.0 45-49 36.99379619724961 38.0 38.0 39.0 35.8 39.0 50-54 36.935282893405414 38.0 38.0 39.0 35.8 39.0 55-59 36.888064124432624 38.0 38.0 39.0 34.8 39.0 60-64 37.010170381720584 38.0 38.0 39.0 34.6 39.0 65-69 36.86543843418428 38.0 38.0 39.0 34.0 39.0 70-74 36.54704241232762 38.0 38.0 39.0 33.0 39.0 75-79 36.39572455597637 38.0 38.0 39.0 32.6 39.0 80-84 36.30389912014417 38.0 38.0 39.0 32.0 39.0 85-89 36.216320699258915 38.0 38.0 39.0 31.2 39.0 90-94 36.10627897115653 38.0 37.0 39.0 31.0 39.0 95-99 35.98190898840672 38.0 37.0 39.0 30.4 39.0 100-104 35.9086260564534 38.0 37.0 39.0 29.6 39.0 105-109 35.77611716634383 38.0 37.0 39.0 28.2 39.0 110-114 35.58367400040475 38.0 37.0 39.0 26.4 39.0 115-119 35.44964873225592 38.0 37.0 39.0 26.0 39.0 120-124 35.304604064876116 38.0 37.0 39.0 25.6 39.0 125-129 35.132003430763156 38.0 37.0 39.0 24.6 39.0 130-134 35.0037430011468 38.0 37.0 39.0 24.2 39.0 135-139 34.829524800755536 38.0 37.0 39.0 24.0 39.0 140-144 34.67447984426648 38.0 37.0 39.0 24.0 39.0 145-149 34.43234988001966 38.0 36.2 39.0 17.4 39.0 150-151 32.77303357522141 37.5 31.0 38.5 14.5 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.028102551923097963 1101 2 -0.023574437847045715 1101 3 0.006233069631136345 1101 4 0.012499932887116927 1101 5 0.06540968105646527 1101 6 0.06996893297163354 1101 7 -0.0620073727119248 1101 8 0.14335708666614266 1101 9 0.18785658625470347 1101 10-14 0.1959903562912544 1101 15-19 0.12242285838527778 1101 20-24 0.08337711228286082 1101 25-29 0.1445640123160601 1101 30-34 0.09012597671821965 1101 35-39 0.15517565293720992 1101 40-44 0.16869284735325607 1101 45-49 0.22289824737240593 1101 50-54 0.21105998508011936 1101 55-59 0.23974589138779834 1101 60-64 0.27682152381463254 1101 65-69 0.2892623956090645 1101 70-74 0.27524129788352525 1101 75-79 0.3399702252230341 1101 80-84 0.2875349404569505 1101 85-89 0.2861789344457293 1101 90-94 0.34343553272915983 1101 95-99 0.257064536340188 1101 100-104 0.269803569891387 1101 105-109 0.2880726465323775 1101 110-114 0.22128121481918583 1101 115-119 0.2280025310710272 1101 120-124 0.24474546756874815 1101 125-129 0.3073508965378764 1101 130-134 0.3009607487059185 1101 135-139 0.20888911315550018 1101 140-144 0.22131172876640193 1101 145-149 0.18026173668189927 1101 150-151 -0.04739081991122873 1102 1 -0.05361475343995892 1102 2 -0.007442959181858555 1102 3 0.004893030284534916 1102 4 0.018680668624647012 1102 5 -7.453098182921281E-4 1102 6 0.04082005706106884 1102 7 -0.005356594124449998 1102 8 0.052224120311201716 1102 9 0.0555560004405109 1102 10-14 0.06572000838662717 1102 15-19 0.09799575317003928 1102 20-24 0.04733036705722071 1102 25-29 0.08003765022981923 1102 30-34 0.08199509223408796 1102 35-39 0.18033537265198873 1102 40-44 0.13063340329732398 1102 45-49 0.09953767030090432 1102 50-54 0.03565245875695666 1102 55-59 0.08897683408198276 1102 60-64 0.0924108122694065 1102 65-69 0.029188364587270144 1102 70-74 0.028124888061462627 1102 75-79 0.020212855118479922 1102 80-84 0.1376899957609794 1102 85-89 0.04545811408214462 1102 90-94 0.01213977508344044 1102 95-99 0.09159960980073834 1102 100-104 -0.032549753965476214 1102 105-109 -0.12221572367105438 1102 110-114 -0.09546480275837865 1102 115-119 -0.04033473289525347 1102 120-124 5.262272882831098E-4 1102 125-129 0.09684633033702994 1102 130-134 0.007980004556777942 1102 135-139 -0.06003597842130404 1102 140-144 0.02641533237605387 1102 145-149 0.013750460888388716 1102 150-151 0.13435542809045842 1103 1 -0.20859932755129051 1103 2 -0.20274544073785705 1103 3 -0.17301709714671887 1103 4 -0.1647680235282749 1103 5 -0.1886205613273475 1103 6 -0.20540931919647676 1103 7 -0.12396289861808185 1103 8 -0.1647363089309195 1103 9 -0.13028839929121716 1103 10-14 -0.09907355298158649 1103 15-19 -0.05444207379173349 1103 20-24 -0.08507741295619553 1103 25-29 -0.022068587194731037 1103 30-34 0.008786239115366357 1103 35-39 0.04449955776264858 1103 40-44 0.008021733277210785 1103 45-49 0.044359132406874835 1103 50-54 0.008971036892440054 1103 55-59 -0.003004386575291562 1103 60-64 0.12890122139079807 1103 65-69 -0.00884007270314413 1103 70-74 -0.034237821529416124 1103 75-79 -0.02838325487481086 1103 80-84 -0.060980158497912385 1103 85-89 0.04452182970923957 1103 90-94 0.047015697283313784 1103 95-99 0.019323553462712084 1103 100-104 0.03490430371393671 1103 105-109 0.03216764319681431 1103 110-114 -0.010979759163468827 1103 115-119 -0.07190656388116423 1103 120-124 -0.2090936251597384 1103 125-129 -0.0839939111116621 1103 130-134 -0.09228639383355386 1103 135-139 -0.04139694421607487 1103 140-144 0.0651276060179029 1103 145-149 0.0645340960325882 1103 150-151 0.00481887544123083 1104 1 -0.16710390446578316 1104 2 -0.09086243113750214 1104 3 -0.10478400940391452 1104 4 -0.11582450935100042 1104 5 -0.06501752493529978 1104 6 0.14659603914243746 1104 7 0.005373560412955669 1104 8 0.18477742181254797 1104 9 -0.016895677701576517 1104 10-14 -0.0016150131289407454 1104 15-19 0.025426468293552773 1104 20-24 0.09857518807081789 1104 25-29 0.09881277025112212 1104 30-34 0.056139130385744807 1104 35-39 0.12991433784749518 1104 40-44 0.17983821016937185 1104 45-49 0.0791956584171487 1104 50-54 0.018026183130430695 1104 55-59 0.06342472706614899 1104 60-64 0.21016561076342555 1104 65-69 0.2238385609204343 1104 70-74 0.0691542749206846 1104 75-79 0.08128390297292754 1104 80-84 -0.02710947307704714 1104 85-89 -0.0016215058567325968 1104 90-94 0.037192075939252334 1104 95-99 0.10975632863574702 1104 100-104 0.1794441563596081 1104 105-109 8.288622189240868E-4 1104 110-114 0.10336344244402795 1104 115-119 -0.11387164537257632 1104 120-124 0.22515526968285826 1104 125-129 0.03602019918081112 1104 130-134 0.03131860728276337 1104 135-139 -0.004255885944139948 1104 140-144 0.041175831068137825 1104 145-149 0.07667752090421232 1104 150-151 0.304660581179121 1105 1 0.04179345096037679 1105 2 0.11027789699882362 1105 3 0.06625370866569824 1105 4 0.10462079197150587 1105 5 0.09732483717551332 1105 6 0.029010760063762575 1105 7 0.22399042590856055 1105 8 0.19546976768730673 1105 9 0.15201631228958945 1105 10-14 0.10527836247415934 1105 15-19 0.1832808676437594 1105 20-24 0.23316124842645536 1105 25-29 0.2876066219642368 1105 30-34 0.2592514086442037 1105 35-39 0.25635653706692096 1105 40-44 0.3263546262758865 1105 45-49 0.3386643723892675 1105 50-54 0.311587505408518 1105 55-59 0.3289920907474766 1105 60-64 0.2419425247813649 1105 65-69 0.20781884407361417 1105 70-74 0.2592041863966088 1105 75-79 0.373422469475706 1105 80-84 0.3830503126601741 1105 85-89 0.48757599087172565 1105 90-94 0.45397298482183146 1105 95-99 0.5070169044094328 1105 100-104 0.46185542656730405 1105 105-109 0.40229815838836913 1105 110-114 0.5012865552354739 1105 115-119 0.34309193898101853 1105 120-124 0.4384181317755349 1105 125-129 0.29581973926262606 1105 130-134 0.34332487873358986 1105 135-139 0.19380569779184498 1105 140-144 0.29453315423454995 1105 145-149 0.42169376137825054 1105 150-151 0.24766740032013246 1106 1 -0.09025726352621888 1106 2 -0.18639173579046542 1106 3 -0.15645833287539546 1106 4 -0.17083160087324956 1106 5 -0.3345542976156821 1106 6 -0.08135827822938779 1106 7 -0.08596110770695731 1106 8 -0.6414643143036542 1106 9 -0.4399642443773999 1106 10-14 -0.31458141060414846 1106 15-19 -0.3365603634715981 1106 20-24 -0.06825628020490626 1106 25-29 -0.11232719476132758 1106 30-34 -0.09113925898178366 1106 35-39 -0.0955016958751358 1106 40-44 0.012794063700305003 1106 45-49 0.024165355823036805 1106 50-54 -0.038681974076887116 1106 55-59 0.0317231710696646 1106 60-64 0.14881919766122564 1106 65-69 0.17518383596038944 1106 70-74 0.19799938464922917 1106 75-79 0.08402811741083838 1106 80-84 0.04880188761805471 1106 85-89 0.12140833071662627 1106 90-94 0.13651648080692524 1106 95-99 0.014025411533005183 1106 100-104 0.13447045549365555 1106 105-109 0.023448771470825136 1106 110-114 -0.09563308354224631 1106 115-119 0.10646890063640058 1106 120-124 0.11202981338962559 1106 125-129 0.010757286560156842 1106 130-134 0.1487157104870107 1106 135-139 0.018083781102923524 1106 140-144 0.13317327425161807 1106 145-149 0.2203655712956234 1106 150-151 0.004559864919627898 1107 1 -0.09340022240587587 1107 2 -0.15390427742689639 1107 3 -0.28780904345543945 1107 4 -0.09413267677211934 1107 5 -0.08196731747947439 1107 6 -0.017543547384399005 1107 7 -0.1369069702064465 1107 8 -0.4376759062463549 1107 9 -0.4276083098057555 1107 10-14 -0.2884641570084341 1107 15-19 -0.11484117893714796 1107 20-24 -0.0840483301821422 1107 25-29 -0.09238894513656959 1107 30-34 -0.0714267362761376 1107 35-39 -0.08111475991072581 1107 40-44 -0.11234068283017962 1107 45-49 -0.20644534186605767 1107 50-54 -0.1706844440918971 1107 55-59 -0.17090595257081276 1107 60-64 -0.17195425959354793 1107 65-69 -0.22390315995146182 1107 70-74 -0.24397473428109606 1107 75-79 -0.24850495509852522 1107 80-84 -0.39893899518694553 1107 85-89 -0.40122313673371224 1107 90-94 -0.4430961595399623 1107 95-99 -0.5419265583941169 1107 100-104 -0.4401695217894215 1107 105-109 -0.4317142359978092 1107 110-114 -0.3688374772842806 1107 115-119 -0.4457070113084569 1107 120-124 -0.44610839026040594 1107 125-129 -0.3332162824763145 1107 130-134 -0.2753906443928429 1107 135-139 -0.4214049979073593 1107 140-144 -0.4473556154586049 1107 145-149 -0.39348111649538 1107 150-151 -0.38586792545362414 1108 1 -0.2312350594499719 1108 2 -0.24342779895350475 1108 3 -0.2651166264002498 1108 4 -0.23845957250831873 1108 5 -0.23684696796263438 1108 6 -0.054571390480944615 1108 7 -0.007401141164592673 1108 8 -0.31617141316763764 1108 9 -0.1130111072042368 1108 10-14 -0.09254335795804991 1108 15-19 -0.11162638224275412 1108 20-24 -0.17070395409046313 1108 25-29 -0.19747580530726339 1108 30-34 -0.23017309735627123 1108 35-39 -0.26811304030952954 1108 40-44 -0.20838848864612203 1108 45-49 -0.1487869783306266 1108 50-54 -0.17199242421493466 1108 55-59 -0.26684119446588994 1108 60-64 -0.10020238766546186 1108 65-69 -0.27990745669775663 1108 70-74 -0.10712309388987507 1108 75-79 -0.21405624299406867 1108 80-84 -0.13608860951785573 1108 85-89 -0.1634324406337342 1108 90-94 -0.1167144080742375 1108 95-99 -0.11473257744963661 1108 100-104 -0.22610309373058612 1108 105-109 -0.18745522163168715 1108 110-114 -0.2210203778588351 1108 115-119 -0.027166460765272404 1108 120-124 -0.08134486927539797 1108 125-129 -0.022839730434682792 1108 130-134 -0.20687478208731136 1108 135-139 -0.03969929794842386 1108 140-144 -0.06738080016904746 1108 145-149 -0.1643935883431169 1108 150-151 0.07044743406036957 1109 1 -0.21905724000920657 1109 2 -0.13159392558634408 1109 3 -0.2178386459682642 1109 4 -0.19785219861244485 1109 5 -0.11934783405462213 1109 6 -0.19606251784967554 1109 7 -0.1340167706719555 1109 8 -0.17047368266829466 1109 9 -0.22356112656394345 1109 10-14 -0.16606883917687298 1109 15-19 -0.09349392564357828 1109 20-24 -0.09389896177773949 1109 25-29 -0.15207936160550872 1109 30-34 -0.16554103361189476 1109 35-39 -0.17519606513297248 1109 40-44 -0.08565503439956501 1109 45-49 -0.21944423459649443 1109 50-54 -0.1463353604139641 1109 55-59 -0.11449260206350687 1109 60-64 -0.1049115732219974 1109 65-69 -0.18417071243378302 1109 70-74 -0.08781492590652107 1109 75-79 -0.020014231305779617 1109 80-84 -0.07959160630935003 1109 85-89 0.037289506476916756 1109 90-94 -0.06261393234632351 1109 95-99 -0.09111011657095958 1109 100-104 -0.09673879392915552 1109 105-109 -0.19852326589408875 1109 110-114 -0.12560063458435167 1109 115-119 -0.5315321531067525 1109 120-124 -0.2458801571462672 1109 125-129 -0.47470906262680757 1109 130-134 -0.47291804366520296 1109 135-139 -0.14383330785244652 1109 140-144 -0.09985555896525966 1109 145-149 -0.21443156053307177 1109 150-151 -0.38368954206718797 1110 1 -0.15777777872974497 1110 2 -0.17873197272439256 1110 3 -0.1659867941164137 1110 4 -0.1547545555148062 1110 5 -0.2109303256371149 1110 6 -0.1381500599372174 1110 7 -0.08283831949350429 1110 8 -0.05195516414977419 1110 9 -0.1070628100656279 1110 10-14 -0.1306479637559974 1110 15-19 -0.18777001991967524 1110 20-24 -0.21470704258581463 1110 25-29 -0.22548003500617853 1110 30-34 -0.23153429960517258 1110 35-39 -0.1885293984663079 1110 40-44 -0.1769681657126867 1110 45-49 -0.4366832918355499 1110 50-54 -0.27064512472371405 1110 55-59 -0.23866768623859258 1110 60-64 -0.45009675840717733 1110 65-69 -0.19400236226542944 1110 70-74 -0.1375118956034882 1110 75-79 -0.16425665554820768 1110 80-84 -0.10033234705009164 1110 85-89 -0.07867008948267795 1110 90-94 -0.14113245086483772 1110 95-99 -0.03198553744638133 1110 100-104 -0.12623374342410898 1110 105-109 -0.1799374073082305 1110 110-114 -0.07523026421397816 1110 115-119 -0.3293772709518734 1110 120-124 -0.25153672280282535 1110 125-129 -0.35403566195341085 1110 130-134 -0.17473622548338597 1110 135-139 0.035021911002779404 1110 140-144 -0.13379495290465826 1110 145-149 -0.08177162787313819 1110 150-151 -0.07055822893588015 1111 1 -0.09160044687074276 1111 2 -0.02386655110275626 1111 3 -0.09131394066585585 1111 4 -0.07949513344544101 1111 5 -0.11360063744107407 1111 6 -0.06792644478412058 1111 7 -0.13565889145443322 1111 8 -0.11359325990472513 1111 9 -0.1941616923290468 1111 10-14 -0.15366804592986938 1111 15-19 -0.11332329161130161 1111 20-24 -0.06664446019146908 1111 25-29 -0.03401960577543406 1111 30-34 0.07998229911088828 1111 35-39 -0.03089457700341569 1111 40-44 -0.08458672877785745 1111 45-49 -0.22029401288079242 1111 50-54 -0.09724476789824621 1111 55-59 -0.021735526042498066 1111 60-64 -0.2405696539170208 1111 65-69 -0.21165296726324812 1111 70-74 -0.1153652896288122 1111 75-79 -0.06493091549715047 1111 80-84 -0.11560375375199072 1111 85-89 -0.162674946650732 1111 90-94 -0.06146874417628112 1111 95-99 -0.04601488396733089 1111 100-104 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-0.15056854238009265 2112 130-134 -0.03455905096993206 2112 135-139 0.015201285872421977 2112 140-144 -0.02845986845452586 2112 145-149 -0.19431700448931366 2112 150-151 -0.060281419544025994 2113 1 0.2374794725382543 2113 2 0.09701994990462737 2113 3 0.04837712716490472 2113 4 0.13944375171238477 2113 5 0.07578821413716952 2113 6 0.09056918987000984 2113 7 0.010076501426688367 2113 8 0.13489565883268284 2113 9 0.05029767637478244 2113 10-14 -0.041512236661915836 2113 15-19 -0.06671482955622565 2113 20-24 -0.1094920088818725 2113 25-29 -0.1409369999997523 2113 30-34 -0.055947855355192644 2113 35-39 0.021086618429762893 2113 40-44 -0.10177947558780431 2113 45-49 -0.012503549482531184 2113 50-54 -0.05802381423806935 2113 55-59 -0.023347346055921037 2113 60-64 0.057885685236776396 2113 65-69 0.15866268082272228 2113 70-74 0.05266694677145978 2113 75-79 -0.1561330645904988 2113 80-84 -0.17228609089101354 2113 85-89 -0.16589858667759927 2113 90-94 -0.0922276427659412 2113 95-99 -0.01404712036760003 2113 100-104 -0.17835694544657343 2113 105-109 -0.125913216440523 2113 110-114 -0.21745981289080163 2113 115-119 -0.09232223435352438 2113 120-124 -0.05103264463589596 2113 125-129 -0.07977327086093311 2113 130-134 -0.11669731839143083 2113 135-139 -0.22825604428323487 2113 140-144 -0.22290463038236652 2113 145-149 -0.14712939176683903 2113 150-151 -0.3708598798755389 2114 1 0.16974621745097096 2114 2 0.21381539146476314 2114 3 0.14930040624541618 2114 4 0.17772948890954154 2114 5 0.13334325594990304 2114 6 -0.09035368826025092 2114 7 0.056264147967176825 2114 8 0.16744279554669106 2114 9 0.19222358623569846 2114 10-14 0.13798262069548173 2114 15-19 0.10562160780030894 2114 20-24 0.08245763905647152 2114 25-29 0.13501345712909796 2114 30-34 0.128959192530111 2114 35-39 0.18119571334835172 2114 40-44 0.04628224888100618 2114 45-49 0.11354737302198714 2114 50-54 0.14168112282640521 2114 55-59 0.07647277308097244 2114 60-64 0.017127725235212665 2114 65-69 -0.06864631422281775 2114 70-74 -0.1380104131169375 2114 75-79 0.14774193734590568 2114 80-84 0.1656327768254755 2114 85-89 0.11042685006201935 2114 90-94 0.0011993246012593772 2114 95-99 -0.02483040402259462 2114 100-104 -0.026992575526229245 2114 105-109 0.003294915924094255 2114 110-114 0.030091611257155648 2114 115-119 0.08742658853855545 2114 120-124 -0.04822198336869121 2114 125-129 0.1680227329408055 2114 130-134 0.2329606437060221 2114 135-139 0.09839192959667287 2114 140-144 -0.01593073862252936 2114 145-149 -0.018661923867917096 2114 150-151 -0.11174874102191268 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 5.0 14 74.0 15 207.0 16 1899.0 17 4186.0 18 2370.0 19 1680.0 20 1236.0 21 1204.0 22 1336.0 23 1696.0 24 2155.0 25 2690.0 26 3603.0 27 4643.0 28 5644.0 29 5975.0 30 5542.0 31 5827.0 32 6849.0 33 8706.0 34 11366.0 35 16941.0 36 34214.0 37 98820.0 38 186200.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.11226112347856 12.619137105245407 6.089122746152437 53.1794790251236 2 25.509795985236156 15.00958879027051 38.218797883720256 21.261817340773078 3 22.271049562963178 19.680148794896258 23.813206510740407 34.23559513140015 4 27.097969489336688 26.3050873591797 19.270818275559666 27.326124875923945 5 31.38184586621951 25.228878159723227 20.64794202395752 22.741333950099744 6 25.737469523066096 30.132171114130696 21.423477598851274 22.70688176395193 7 22.587865120895852 17.746971580560295 33.85372999123035 25.811433307313504 8 21.93977854231114 19.71869669548122 27.326606724681255 31.014918037526378 9 25.393188585966637 18.562500602310948 28.58664122505228 27.457669586670136 10-14 27.696666570296912 22.759355093623213 22.668285678491234 26.875692657588633 15-19 27.837800071313616 22.057253269343818 22.699798587219444 27.40514807212312 20-24 28.69086511125888 21.955101332793664 22.25924426840903 27.09478928753843 25-29 27.64163944221188 23.64296934478206 21.7723360991452 26.943055113860858 30-34 28.745024911580753 22.508408260815095 21.49291200477994 27.253654822824213 35-39 27.740514807212314 21.403239951044167 22.62973777790627 28.22650746383725 40-44 29.53862981487371 21.14993205932522 21.58639066369848 27.725047462102598 45-49 28.97958888664026 21.410515867279578 21.599063286015785 28.010831960064376 50-54 28.718571414804323 22.175450769512466 21.212283288521398 27.893694527161816 55-59 28.36826736823846 21.44217333063498 21.85425038788825 28.33530891323831 60-64 27.980764597608104 22.557364094558 20.913537058987924 28.548334248845975 65-69 28.67751790068133 22.199735946880995 21.085797989726984 28.03694816271069 70-74 28.568957375658925 21.887160658012665 20.465851378569294 29.078030587759113 75-79 28.976119575587617 21.41586438848574 20.939075043125463 28.66894099280118 80-84 28.627887478678193 20.203291990709957 21.83892759740573 29.329892933206125 85-89 28.967446297955995 21.17836113600663 20.979502153863947 28.874690412173425 90-94 29.142839245617587 20.841307930266847 21.03327647517997 28.982576348935595 95-99 29.239656750053843 21.03603314928308 20.866181380487717 28.85812872017535 100-104 28.11924793045959 21.366908554742835 21.373365328090816 29.14047818670676 105-109 28.121753543997606 21.458797112762245 21.695433037478196 28.724016305761946 110-114 29.476953173937765 21.51286054333266 20.719737488797016 28.29044879393256 115-119 28.521928936945272 21.823604806923203 20.526130658109032 29.128335598022492 120-124 29.00316526601196 21.729751139452397 20.818430145629222 28.44865344890642 125-129 30.09050569569598 22.229958575442375 20.00010600677769 27.67942972208396 130-134 30.0670396499078 21.874932239985853 20.200121522315147 27.8579065877912 135-139 29.68602734973547 22.57644530534756 20.14860215675504 27.588925188161937 140-144 29.740042595430143 22.669731224763172 20.38533445122245 27.20489172858423 145-149 29.88404780329758 22.361562309368402 20.837810515684673 26.91657937164934 150-151 30.284676245819963 22.959972823730087 19.62064047336822 27.134710457081727 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 1.0 3 1.5 4 1.0 5 1.5 6 1.0 7 0.5 8 1.5 9 3.0 10 2.5 11 1.5 12 1.5 13 2.5 14 6.0 15 7.0 16 6.0 17 15.0 18 26.0 19 34.5 20 61.5 21 94.0 22 146.0 23 185.5 24 214.0 25 278.0 26 369.0 27 470.5 28 610.0 29 733.0 30 817.5 31 973.5 32 1186.5 33 1347.5 34 1615.0 35 1999.0 36 2296.0 37 2676.5 38 3243.5 39 3757.5 40 4327.0 41 5130.5 42 6213.5 43 7610.5 44 9428.5 45 11188.5 46 13091.0 47 14817.0 48 15851.5 49 14735.0 50 12610.0 51 12391.5 52 12606.5 53 13217.5 54 14074.5 55 14653.0 56 15334.5 57 13784.5 58 12278.5 59 13109.0 60 12837.5 61 11554.0 62 11178.5 63 9407.5 64 7552.5 65 7134.0 66 7666.0 67 7616.0 68 5851.5 69 5228.5 70 5179.5 71 5004.0 72 4337.0 73 4046.0 74 5700.0 75 6475.5 76 5853.5 77 4859.0 78 3791.0 79 3455.5 80 2412.0 81 1321.5 82 1233.5 83 1551.0 84 1920.5 85 1692.0 86 1179.5 87 1084.5 88 983.0 89 996.5 90 1066.0 91 1202.0 92 1134.0 93 707.0 94 622.5 95 467.5 96 137.5 97 81.0 98 140.0 99 378.0 100 337.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 4.818487573120549E-5 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 4.818487573120549E-5 125-129 4.818487573120549E-5 130-134 4.818487573120549E-5 135-139 0.0 140-144 0.0 145-149 9.636975146241099E-5 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 415068.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.711415489003684 #Duplication Level Percentage of deduplicated Percentage of total 1 76.55630483084384 18.91814656977802 2 7.892716706355407 3.900804037354984 3 3.9207151772373305 2.906592652762632 4 2.219890983713709 2.1942659375537032 5 1.3664814990003142 1.6883846039916672 6 0.9519220085825736 1.4114004160326545 7 0.6572556995250088 1.1369203071434777 8 0.49344120906588806 0.9754904589298794 9 0.4774593075576812 1.0618825795334872 >10 4.151298916756742 22.20474967216956 >50 0.757142583951302 13.12271628505772 >100 0.5214095367052501 23.925680714593422 >500 0.02996606532788794 4.909787631218176 >1k 0.003995475377051725 1.643178133880619 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT 2500 0.6023109466400686 No Hit CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCACTCCGC 1648 0.39704337602513323 No Hit CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC 1284 0.30934690219433925 No Hit CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT 1225 0.2951323638536336 No Hit CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG 948 0.22839631096591403 No Hit CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC 899 0.2165910164117687 No Hit CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA 869 0.20936328505208784 No Hit CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC 849 0.2045447974789673 No Hit CTACGAATGGTTTAGCACCAGGTTCCCCACGAACGTGCGTTGCGTGACGG 805 0.19394412481810208 No Hit CTCTTATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG 784 0.18888471286632552 No Hit CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG 780 0.18792101535170141 No Hit CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG 761 0.1833434521572369 No Hit CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT 675 0.16262395559281853 No Hit CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG 669 0.16117840932088237 No Hit CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC 668 0.16093748494222634 No Hit CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA 659 0.15876916553432208 No Hit CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT 657 0.15828731677701005 No Hit CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA 641 0.1544325267185136 No Hit GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA 641 0.1544325267185136 No Hit CTCTCGCGCGCGTCACCGACTGCCAGCGACGGCCGGGTATGGGCCCGACG 631 0.15202328293195333 No Hit ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA 621 0.14961403914539304 No Hit CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT 614 0.14792756849480085 No Hit GTCTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCA 609 0.14672294660152072 No Hit CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA 607 0.14624109784420866 No Hit GGAGTCTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGC 604 0.14551832470824058 No Hit CTCCCTTTCGATCGGCTGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG 599 0.14431370281496042 No Hit AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG 561 0.13515857642603138 No Hit AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG 557 0.13419487891140727 No Hit GTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAC 556 0.13395395453275125 No Hit CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCATGCTC 554 0.13347210577543922 No Hit CTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGC 538 0.12961731571694277 No Hit CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG 520 0.12528067690113426 No Hit CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT 513 0.12359420625054209 No Hit CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA 502 0.12094403808532578 No Hit CTAACACGTGCGCTCATGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGG 499 0.1202212649493577 No Hit GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG 493 0.11877571867742152 No Hit CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT 486 0.11708924802682934 No Hit CCCACCCCGCGGACGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGA 480 0.11564370175489316 No Hit CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT 472 0.11371630672564496 No Hit CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA 467 0.11251168483236482 No Hit CTACTCTTATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCC 463 0.11154798731774071 No Hit CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT 462 0.11130706293908468 No Hit CTCCCGTCCCTCTCGCGCGCGTCACCGACTGCCAGCGACGGCCGGGTATG 460 0.11082521418177263 No Hit CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT 459 0.1105842898031166 No Hit CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA 455 0.10962059228849248 No Hit CTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTC 455 0.10962059228849248 No Hit CCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACC 444 0.10697042412327619 No Hit CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT 441 0.1062476509873081 No Hit CTCGCGCGCGTCACCGACTGCCAGCGACGGCCGGGTATGGGCCCGACGCT 434 0.10456118033671591 No Hit CGACGGGCGCTGACCCCCCTCGCGGGGGGGATGCGTGCATTTATCAGATC 428 0.10311563406477973 No Hit CTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTA 428 0.10311563406477973 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0045775631944645215 0.0 0.0 0.0 0.0 2 0.0045775631944645215 0.0 0.0 0.0 0.0 3 0.005541260709088632 0.0 0.0 0.0 0.0 4 0.006504958223712741 0.0 0.0 0.0 0.0 5 0.0067458826023687685 0.0 0.0 0.0 0.0 6 0.006986806981024796 0.0 0.0 0.0 0.0 7 0.006986806981024796 0.0 0.0 0.0 0.0 8 0.006986806981024796 0.0 0.0 0.0 0.0 9 0.007468655738336851 0.0 0.0 0.0 0.0 10-14 0.008480538128692167 0.0 0.0 0.0 0.0 15-19 0.009974269276359537 0.0 0.0 0.0 0.0 20-24 0.012528067690113425 0.0 0.0 0.0 0.0 25-29 0.015660084612641786 0.0 0.0 0.0 0.0 30-34 0.019611244422600635 0.0 0.0 0.0 0.0 35-39 0.022213227712085733 0.0 0.0 0.0 0.0 40-44 0.0289591103144545 0.0 0.0 0.0 0.0 45-49 0.03613865679840411 0.0 0.0 0.0 0.0 50-54 0.047365732843775 0.0 0.0 0.0 0.0 55-59 0.06177301068740544 0.0 0.0 0.0 0.0 60-64 0.08123970048281245 0.0 0.0 0.0 0.0 65-69 0.1088496342767932 0.0 0.0 0.0 0.0 70-74 0.14816849287345688 0.0 0.0 0.0 0.0 75-79 0.21186889859011054 0.0 0.0 0.0 0.0 80-84 0.3051548180057243 0.0 0.0 0.0 0.0 85-89 0.44296356259697206 0.0 0.0 0.0 0.0 90-94 0.6409070321007642 0.0 0.0 0.0 0.0 95-99 0.9110796303256334 0.0 0.0 0.0 0.0 100-104 1.3117850569063383 0.0 0.0 0.0 0.0 105-109 1.8662002370695885 0.0 0.0 0.0 0.0 110-114 2.609885609105014 0.0 0.0 0.0 0.0 115-119 3.5454913411778306 0.0 0.0 0.0 0.0 120-124 4.725105283953473 0.0 0.0 0.0 0.0 125-129 6.159424479844266 0.0 0.0 0.0 0.0 130-134 8.107153526651055 0.0 0.0 0.0 0.0 135-139 10.08157699461293 0.0 0.0 0.0 0.0 140 11.30103982961828 0.0 0.0 0.0 0.0 >>END_MODULE