##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename DRR051428_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 415068 Sequences flagged as poor quality 0 Sequence length 151 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.628930681237776 34.0 33.0 34.0 32.0 35.0 2 33.76904507213276 34.0 33.0 35.0 32.0 35.0 3 33.87083321287114 34.0 33.0 35.0 32.0 35.0 4 33.89744571973749 34.0 33.0 35.0 32.0 35.0 5 33.80084227162778 34.0 33.0 35.0 32.0 35.0 6 35.46762940048377 37.0 34.0 37.0 33.0 37.0 7 35.61130224445151 37.0 34.0 37.0 33.0 37.0 8 35.5805072903717 37.0 34.0 37.0 33.0 37.0 9 35.63375157805468 37.0 34.0 37.0 33.0 37.0 10-14 36.422546667052146 37.4 35.6 37.4 35.0 37.4 15-19 37.609465918837394 38.0 38.0 38.0 38.0 38.0 20-24 37.80786184432431 38.4 38.0 38.4 38.0 38.4 25-29 38.10902743646824 39.0 38.0 39.0 38.0 39.0 30-34 38.02879865467827 39.0 38.0 39.0 37.4 39.0 35-39 37.96064500274654 39.0 38.0 39.0 37.0 39.0 40-44 37.912172463307215 39.0 38.0 39.0 37.0 39.0 45-49 37.87732130638835 39.0 38.0 39.0 37.0 39.0 50-54 37.83809978124067 39.0 38.0 39.0 37.0 39.0 55-59 37.772529320496886 39.0 38.0 39.0 37.0 39.0 60-64 37.70698777067854 39.0 38.0 39.0 37.0 39.0 65-69 37.61407528404984 39.0 38.0 39.0 37.0 39.0 70-74 37.47344097834572 38.2 38.0 39.0 36.8 39.0 75-79 37.13930729422649 38.0 38.0 39.0 35.4 39.0 80-84 37.06770071409986 38.0 38.0 39.0 35.0 39.0 85-89 36.98830360326501 38.0 38.0 39.0 35.0 39.0 90-94 36.93702814960441 38.0 38.0 39.0 34.8 39.0 95-99 36.78451675388129 38.0 38.0 39.0 33.8 39.0 100-104 36.71845336185878 38.0 38.0 39.0 33.6 39.0 105-109 36.6690354351576 38.0 37.2 39.0 33.6 39.0 110-114 36.489496660788106 38.0 37.0 39.0 33.0 39.0 115-119 36.39377981439186 38.0 37.0 39.0 33.0 39.0 120-124 36.285372035425524 38.0 37.0 39.0 32.0 39.0 125-129 36.179043433846985 38.0 37.0 39.0 31.6 39.0 130-134 36.06643007892682 38.0 37.0 39.0 31.4 39.0 135-139 35.9250676997504 38.0 37.0 39.0 31.0 39.0 140-144 35.791800379696824 38.0 37.0 39.0 30.2 39.0 145-149 35.638143147628824 38.0 37.0 39.0 28.4 39.0 150-151 34.69442235970973 37.5 35.5 38.5 21.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.023121395522522903 1101 2 -0.09587702264965259 1101 3 0.07250719043537401 1101 4 0.06559166460096577 1101 5 0.021110374030527623 1101 6 0.06918623356851583 1101 7 0.04286577042064721 1101 8 0.07930760167438677 1101 9 0.05512728306787551 1101 10-14 0.055822000830055174 1101 15-19 0.0779029737196737 1101 20-24 0.070796729782856 1101 25-29 0.043720329787056755 1101 30-34 0.14923327767620975 1101 35-39 0.09201966587757937 1101 40-44 0.13853505720117454 1101 45-49 0.07818147134654652 1101 50-54 0.10337340986832544 1101 55-59 0.09488565835908247 1101 60-64 0.17153276765836978 1101 65-69 0.16116517974769806 1101 70-74 0.13487780707314556 1101 75-79 0.1643275875734389 1101 80-84 0.12856581033252468 1101 85-89 0.13355442816919094 1101 90-94 0.17657781072738743 1101 95-99 0.17250638348089353 1101 100-104 0.1396836658508107 1101 105-109 0.17935628841839701 1101 110-114 0.21319366194851597 1101 115-119 0.17737972781165467 1101 120-124 0.29500597027512043 1101 125-129 0.22942508045475307 1101 130-134 0.22372772104822047 1101 135-139 0.1478541015031425 1101 140-144 0.23180638525167296 1101 145-149 0.272067751255733 1101 150-151 0.2845347055090315 1102 1 0.021722271773676027 1102 2 -0.008400417410030059 1102 3 0.00784883782918655 1102 4 0.046027090140640325 1102 5 -0.009327087295133651 1102 6 0.03391344495781823 1102 7 -0.005897344179786046 1102 8 0.03923125298854302 1102 9 0.013845570108330207 1102 10-14 0.020657399703168267 1102 15-19 0.00943774606286496 1102 20-24 -0.046325304177635473 1102 25-29 -0.04235502829874349 1102 30-34 0.012751769100631805 1102 35-39 -0.10283350921031342 1102 40-44 -0.088362821062411 1102 45-49 -0.09560234786849975 1102 50-54 -0.12430361514589094 1102 55-59 -0.09690551563619465 1102 60-64 0.19088293582323956 1102 65-69 0.10071307418570541 1102 70-74 0.0866885604079144 1102 75-79 0.034976520083333185 1102 80-84 0.0038175878421427 1102 85-89 -0.008509697747086875 1102 90-94 0.030632984554429754 1102 95-99 0.013256286501416525 1102 100-104 -0.0742944434095989 1102 105-109 -0.15669327558782697 1102 110-114 -0.04725191120130745 1102 115-119 -0.03599274630366267 1102 120-124 -0.04684365986059902 1102 125-129 -0.1499441301390263 1102 130-134 -0.11902114484002624 1102 135-139 -0.18741667369283732 1102 140-144 -0.18095463718716331 1102 145-149 -0.18540960118446037 1102 150-151 0.006630679171777842 1103 1 0.08847344548259173 1103 2 0.10167067581598843 1103 3 0.04254099074669426 1103 4 0.03493922964432983 1103 5 0.05322556193357286 1103 6 -0.008514455779938146 1103 7 0.04109125407641301 1103 8 0.09287042133193779 1103 9 0.07800653590309992 1103 10-14 0.03309120252006892 1103 15-19 0.06330977825603412 1103 20-24 -0.015250857497555614 1103 25-29 -0.047851473637443576 1103 30-34 -0.01355889488326767 1103 35-39 -0.05175316045108502 1103 40-44 0.13702711857541772 1103 45-49 0.14687411088401348 1103 50-54 0.128212280226343 1103 55-59 0.10016571172799615 1103 60-64 0.1138785092638841 1103 65-69 0.06212635386378196 1103 70-74 0.07554865430462598 1103 75-79 0.1313327910424178 1103 80-84 0.12338244364362083 1103 85-89 0.08668922914761623 1103 90-94 0.0117114553793769 1103 95-99 0.014409874697264513 1103 100-104 0.06858711259309302 1103 105-109 -0.06387288658715562 1103 110-114 -0.1104626422542907 1103 115-119 0.013407924387152548 1103 120-124 -0.03896921318052193 1103 125-129 0.014895038204365108 1103 130-134 -0.03418573236516664 1103 135-139 -0.02525517134542099 1103 140-144 0.08860860895636335 1103 145-149 0.10907597896309085 1103 150-151 0.10289761411476661 1104 1 0.13069403043459005 1104 2 0.05956216945295978 1104 3 0.03144610349507815 1104 4 0.06282670452711869 1104 5 0.08873482739461735 1104 6 -0.08399112467925107 1104 7 0.00265856820903565 1104 8 0.07323568143463888 1104 9 0.035091599533373596 1104 10-14 0.03879399332159039 1104 15-19 -0.007201675193400092 1104 20-24 -0.010898993242143717 1104 25-29 0.022786793832977992 1104 30-34 0.0747051819195832 1104 35-39 -3.0756991748859264E-4 1104 40-44 0.013532677852033714 1104 45-49 0.0604763435252309 1104 50-54 0.017673588554096398 1104 55-59 0.08020348801734656 1104 60-64 0.07433807613148957 1104 65-69 -0.07945810695337485 1104 70-74 -0.06059280584272386 1104 75-79 -0.03690940587652847 1104 80-84 -0.09846011049793191 1104 85-89 -0.00456560452060728 1104 90-94 -0.07245286701402165 1104 95-99 -0.03722665131300573 1104 100-104 -0.0378990673577988 1104 105-109 -0.11303314557353161 1104 110-114 -0.154986687800168 1104 115-119 -0.13826244846393365 1104 120-124 -0.243924605009056 1104 125-129 -0.16429871040023158 1104 130-134 -0.19219921751810887 1104 135-139 -0.12516434750081373 1104 140-144 -0.07256066990052545 1104 145-149 -0.1488793682126186 1104 150-151 0.016439476137534825 1105 1 0.03888912139628786 1105 2 0.08960726762576599 1105 3 0.06508348304083711 1105 4 0.06448216227312997 1105 5 0.1370890581907318 1105 6 0.14166608142834036 1105 7 0.14727355262331088 1105 8 0.09724910913466545 1105 9 -0.05531975207576778 1105 10-14 0.06147394140161566 1105 15-19 0.06036869503277842 1105 20-24 0.0941317933170609 1105 25-29 0.05034808602789553 1105 30-34 0.055162021012804985 1105 35-39 0.015210820174182516 1105 40-44 0.03065618066260356 1105 45-49 0.03457802986243763 1105 50-54 0.034466265254955886 1105 55-59 0.09805686100144584 1105 60-64 0.00903553260161516 1105 65-69 -0.16801155894995645 1105 70-74 -0.07959110791465918 1105 75-79 0.08798483859856532 1105 80-84 0.21507456165330296 1105 85-89 0.1527968469371359 1105 90-94 0.17691268831639917 1105 95-99 0.2552200904612505 1105 100-104 0.22061335653848602 1105 105-109 0.24920012089853572 1105 110-114 0.17444409486592605 1105 115-119 0.25032188299569924 1105 120-124 0.3134725305447077 1105 125-129 0.3146787458221638 1105 130-134 0.2576427132535031 1105 135-139 0.35792105058325063 1105 140-144 0.2702806477058175 1105 145-149 0.3561398274879721 1105 150-151 0.4035343380249401 1106 1 0.026628359294996073 1106 2 0.00471701564137561 1106 3 0.02314049824613562 1106 4 0.018436879073476575 1106 5 0.04211251066874411 1106 6 0.06214146541996968 1106 7 0.010372650987093834 1106 8 0.07027986838812694 1106 9 -0.15990884354619794 1106 10-14 0.01012822960760218 1106 15-19 0.02491300189605994 1106 20-24 0.12567504355460102 1106 25-29 0.06264380283446513 1106 30-34 0.06265341763071319 1106 35-39 0.013438332721065649 1106 40-44 -0.018201625548158518 1106 45-49 0.04608637862522613 1106 50-54 -0.005681697835001387 1106 55-59 -0.012166591831444862 1106 60-64 -0.13134575758187594 1106 65-69 -0.08491783686825727 1106 70-74 -0.020167665373001853 1106 75-79 -0.12356902225509714 1106 80-84 -0.04003694296932281 1106 85-89 -0.1179042787995499 1106 90-94 0.006527663125062588 1106 95-99 0.020355699635707936 1106 100-104 0.1010324226445789 1106 105-109 0.0772996229270646 1106 110-114 0.10644051422657697 1106 115-119 0.05379144800479452 1106 120-124 0.1298130567993283 1106 125-129 0.10232678210407897 1106 130-134 0.13917553918172842 1106 135-139 0.06702071851354674 1106 140-144 0.23124671059056823 1106 145-149 0.22510776763557772 1106 150-151 0.32688283909797633 1107 1 0.08390521282040453 1107 2 0.08137537380256532 1107 3 0.08495262385631008 1107 4 0.005731175953052059 1107 5 0.06838887289553952 1107 6 -0.012049131395151846 1107 7 -0.07192888015177346 1107 8 -0.07109378448461712 1107 9 -0.025322323157304538 1107 10-14 0.012913126457426927 1107 15-19 0.02314333530301127 1107 20-24 -0.009577479421494672 1107 25-29 -0.013850578782857781 1107 30-34 -0.04748193738886641 1107 35-39 -0.009233249936549726 1107 40-44 -0.028205096189680035 1107 45-49 -0.059906202315090695 1107 50-54 0.04932995360441339 1107 55-59 -0.07255106679549783 1107 60-64 0.0344404779439742 1107 65-69 0.10198675654834233 1107 70-74 0.08879966101603287 1107 75-79 0.06381064339141318 1107 80-84 0.03378423335278313 1107 85-89 0.0787089159485106 1107 90-94 0.08107163435028752 1107 95-99 0.11606535568160581 1107 100-104 -0.0019247442301804085 1107 105-109 -0.08266125347753217 1107 110-114 0.029542726873231118 1107 115-119 -0.13965426800658065 1107 120-124 -0.4393128373415962 1107 125-129 -0.4889939998269526 1107 130-134 -0.21584837218619413 1107 135-139 -0.19431020490246453 1107 140-144 -0.057609735562650144 1107 145-149 -0.08136249978411314 1107 150-151 0.05070969465167963 1108 1 -0.10900380177138658 1108 2 -0.03929233696141665 1108 3 -0.026086222882540255 1108 4 -0.008555054148544627 1108 5 0.045943338216922314 1108 6 0.01916095332115475 1108 7 -0.08427525567476124 1108 8 -0.007532178981897175 1108 9 0.06264137114183654 1108 10-14 -0.027732558968381227 1108 15-19 -0.00328228630348093 1108 20-24 0.028064094306969878 1108 25-29 0.008274776082352275 1108 30-34 0.03333489574662707 1108 35-39 0.03883262748883709 1108 40-44 -0.004744771135413828 1108 45-49 0.01061757647468653 1108 50-54 -0.08230222324822023 1108 55-59 -0.1701049002424213 1108 60-64 -0.11819376152742223 1108 65-69 -0.012003034165815052 1108 70-74 -0.09516607333491578 1108 75-79 -0.06317319416269385 1108 80-84 -0.024964384421842567 1108 85-89 -0.09820509947824974 1108 90-94 -0.186573234152128 1108 95-99 -0.22551839250740358 1108 100-104 -0.09129282464071053 1108 105-109 -0.24840083996083706 1108 110-114 -0.16487394016955648 1108 115-119 -0.28469662616204516 1108 120-124 -0.17106443846633113 1108 125-129 -0.2858803933132279 1108 130-134 -0.10536295720264377 1108 135-139 -0.21464293193721318 1108 140-144 -0.16647204560538853 1108 145-149 -0.053922900211972546 1108 150-151 -0.14521133006815035 1109 1 -0.05510231209316174 1109 2 -0.12988487973956353 1109 3 -0.016859682653979746 1109 4 -0.09757255486745464 1109 5 -0.1098114751034629 1109 6 -0.06770637497186272 1109 7 0.044390917376077255 1109 8 -0.014173349711832373 1109 9 0.0575082194047809 1109 10-14 0.018614434843300387 1109 15-19 -0.06805183790559255 1109 20-24 -0.05176264200934355 1109 25-29 -0.12879962178558912 1109 30-34 -0.07794812047249877 1109 35-39 -0.03509861499654221 1109 40-44 -0.07475335280506101 1109 45-49 -0.07558649517660143 1109 50-54 -0.14027391505985776 1109 55-59 -0.017626880864597183 1109 60-64 -0.4039962382108655 1109 65-69 -0.04690529596787485 1109 70-74 -0.07299275983678655 1109 75-79 -0.00921401030438318 1109 80-84 -0.0036559401947684478 1109 85-89 0.029021889080276253 1109 90-94 -0.13938618771827294 1109 95-99 -0.14963052934314192 1109 100-104 -0.2654155810095986 1109 105-109 -0.16231867783293552 1109 110-114 -0.25685186784351544 1109 115-119 -0.18746345608422388 1109 120-124 -0.08635507176637702 1109 125-129 -0.1819345914252608 1109 130-134 -0.025711658291093897 1109 135-139 -0.05060062589087977 1109 140-144 -0.009033141885382179 1109 145-149 0.1220524099395277 1109 150-151 -0.1859172007000467 1110 1 -0.09079254778340129 1110 2 -0.03897578883047714 1110 3 -0.08621995201424681 1110 4 -0.12450858180348234 1110 5 -0.1441549094468968 1110 6 -0.09060199786748058 1110 7 -0.0047673317821690375 1110 8 -0.02629456183304768 1110 9 -0.013872251975691086 1110 10-14 -0.06556064933178618 1110 15-19 -0.09848954834329504 1110 20-24 -0.2256346958813893 1110 25-29 -0.06199827498424071 1110 30-34 -0.18326798579236225 1110 35-39 -0.013415113313044458 1110 40-44 -0.012665810717514603 1110 45-49 -0.14521612480622537 1110 50-54 -0.13353990832585083 1110 55-59 -0.07782890106662421 1110 60-64 -0.08709388130851181 1110 65-69 -0.34010394916652587 1110 70-74 -0.2964271032711281 1110 75-79 -0.2598537409441093 1110 80-84 -0.1970566135954428 1110 85-89 -0.1921228920645106 1110 90-94 -0.1997228880549713 1110 95-99 -0.21077531048791798 1110 100-104 -0.313361708955739 1110 105-109 -0.21861497412923825 1110 110-114 -0.1841245951162449 1110 115-119 -0.11554426416503105 1110 120-124 -0.16945271486402902 1110 125-129 -0.13344974294040668 1110 130-134 -0.19581266839210087 1110 135-139 -0.2033278986181415 1110 140-144 -0.1992014920537315 1110 145-149 -0.24037183248471905 1110 150-151 -0.23911585389870282 1111 1 -0.10030246676276278 1111 2 -0.08527626844190195 1111 3 -0.1279676280131028 1111 4 -0.10782235531201678 1111 5 -0.088176273750328 1111 6 -0.07956437742758027 1111 7 0.03253517617178403 1111 8 -0.08149984954336986 1111 9 -0.17401226866372355 1111 10-14 -0.06334389933800111 1111 15-19 -0.03915684363785488 1111 20-24 -0.21495625261732698 1111 25-29 -0.1384307438411554 1111 30-34 -0.15883751588170014 1111 35-39 -0.15875777204723107 1111 40-44 -0.16192224341249783 1111 45-49 -0.3081110693264151 1111 50-54 -0.13637343725743278 1111 55-59 -0.19510816335595393 1111 60-64 -0.10287027067791144 1111 65-69 -0.2782019201456265 1111 70-74 -0.13447256799221208 1111 75-79 -0.1113460538199007 1111 80-84 -0.09430155008819696 1111 85-89 -0.16572160586469664 1111 90-94 -0.03887193391992838 1111 95-99 -0.07702509284247583 1111 100-104 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-0.207839924735147 2111 145-149 -0.1418499220890581 2111 150-151 0.2657679969220226 2112 1 -0.014113802078107085 2112 2 -0.0384545794597102 2112 3 -0.08373584156553449 2112 4 -0.043081192411492 2112 5 -0.08657067133168539 2112 6 0.028703606393875702 2112 7 0.006686897371174894 2112 8 0.013053766010855838 2112 9 0.10579686946334732 2112 10-14 -0.06433734297574034 2112 15-19 -0.04824148110407833 2112 20-24 0.03808156818885777 2112 25-29 0.021345349598313135 2112 30-34 -0.08286490804415081 2112 35-39 0.011677381867947645 2112 40-44 0.06314856173403172 2112 45-49 0.04579972921847997 2112 50-54 0.12573612864930794 2112 55-59 0.13450281179606094 2112 60-64 0.13711590431720566 2112 65-69 0.1460168916445781 2112 70-74 0.071169076360448 2112 75-79 -0.011325343743152416 2112 80-84 0.12342728140172454 2112 85-89 0.10287638594362392 2112 90-94 0.11802504739208075 2112 95-99 -0.012828609532370194 2112 100-104 0.1512186533237454 2112 105-109 0.16105327633547262 2112 110-114 0.17284899831752654 2112 115-119 0.2379182942532836 2112 120-124 0.19349695428033442 2112 125-129 0.22306150065048058 2112 130-134 0.09037537496408987 2112 135-139 0.11947920578187166 2112 140-144 0.2083209756457336 2112 145-149 0.15636302230323196 2112 150-151 0.16654227034349844 2113 1 0.10318980993300642 2113 2 0.06583185781733647 2113 3 0.035900740019137345 2113 4 0.027839162485165048 2113 5 0.030023860568007876 2113 6 0.11049606582292171 2113 7 0.05671247637325649 2113 8 0.017224126279330676 2113 9 0.13223353388832493 2113 10-14 0.02973870643769061 2113 15-19 0.06561145723520667 2113 20-24 0.11788458015635683 2113 25-29 0.08467200968470223 2113 30-34 0.048147037509828294 2113 35-39 0.08301522996753619 2113 40-44 0.1322349090388215 2113 45-49 0.12466260428003295 2113 50-54 0.14878438678555028 2113 55-59 0.08682602066016187 2113 60-64 0.10115927935566305 2113 65-69 0.06793642773885722 2113 70-74 -0.03816632218966731 2113 75-79 -0.08528829693609907 2113 80-84 -0.12962727204924107 2113 85-89 -0.06538581002856603 2113 90-94 -0.11838838791590689 2113 95-99 -0.17628634200298166 2113 100-104 -0.001452466542794184 2113 105-109 -0.04895961145843586 2113 110-114 -0.047622615390622514 2113 115-119 -0.04694730283469539 2113 120-124 -0.11410903856668142 2113 125-129 -0.0740331910322638 2113 130-134 -0.07774857095930088 2113 135-139 -0.0674319342735572 2113 140-144 -0.2677459019010371 2113 145-149 -0.12371694693795376 2113 150-151 0.013921119823294248 2114 1 0.06724051920489416 2114 2 0.048601476358733464 2114 3 0.011945784957454464 2114 4 0.0068849555602028545 2114 5 0.06906183511313202 2114 6 0.08395953788808441 2114 7 0.03577239383218256 2114 8 -0.02151040182500452 2114 9 -0.01405316558943781 2114 10-14 0.04700581129437609 2114 15-19 0.013889968215323734 2114 20-24 -0.03545528988109936 2114 25-29 0.03038425680126977 2114 30-34 0.04618475052604509 2114 35-39 0.04200676718347296 2114 40-44 0.08775330582100338 2114 45-49 0.08796845733358083 2114 50-54 -0.007520510366148869 2114 55-59 0.004517950533958981 2114 60-64 -0.01975984442223222 2114 65-69 -0.11145627842930139 2114 70-74 -0.2163818748676931 2114 75-79 -0.07020099457495377 2114 80-84 -0.05286142790771464 2114 85-89 0.033177876818349716 2114 90-94 -0.09066213123302447 2114 95-99 0.031542393919337997 2114 100-104 -0.007724239736177196 2114 105-109 0.050519153213848256 2114 110-114 0.07459895883119572 2114 115-119 0.032293315612847096 2114 120-124 0.03290921287103998 2114 125-129 0.06624772929436773 2114 130-134 0.04269278414319899 2114 135-139 0.10728268482825598 2114 140-144 -0.1237454389857362 2114 145-149 0.05876480754744762 2114 150-151 0.10671308222873677 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 4.0 17 55.0 18 135.0 19 449.0 20 473.0 21 484.0 22 558.0 23 766.0 24 1098.0 25 1558.0 26 2164.0 27 5723.0 28 5337.0 29 4493.0 30 5254.0 31 6130.0 32 7246.0 33 7969.0 34 8630.0 35 11905.0 36 25653.0 37 96014.0 38 222970.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.537174631626627 12.908969132768608 5.840729711758073 53.7131265238467 2 24.69065309780566 15.155348039357404 39.177195062013936 20.976803800823 3 21.595015756454362 19.301415671648982 24.644877465861015 34.45869110603564 4 26.712490483487045 26.321952065685622 19.316834831882968 27.648722618944365 5 31.63650293445893 25.118052945541454 20.7011863116405 22.54425780835911 6 25.464743126427475 30.473320034307633 21.601279790299422 22.460657048965473 7 20.8310445517361 19.78543274836894 33.95636377653782 25.427158923357133 8 21.648982817273314 19.95721183035069 27.60366976013569 30.790135592240308 9 26.114275251284123 17.871770408704116 28.804195938978673 27.209758401033085 10-14 26.56292462921738 23.29608642439311 22.77207590081625 27.368913045573258 15-19 26.58239131901279 22.676621661992733 22.922750007227734 27.818237011766744 20-24 27.44037121628263 22.702882419266242 22.49139899968198 27.365347364769143 25-29 27.32463114477628 22.81481588558983 22.2547148900903 27.60583807954359 30-34 27.63450808060366 22.70076228473407 21.662330027850857 28.002399606811412 35-39 28.867029016932165 21.313134233426812 22.244933360316864 27.574903389324156 40-44 27.71815702487303 22.05137471450461 22.32916052309501 27.901307737527343 45-49 28.197403798895603 21.919685449131226 22.23288714138406 27.65002361058911 50-54 28.74117012152226 21.98738519953357 21.131477251920167 28.139967427024004 55-59 28.398190176067533 20.81417984523018 21.9466689795407 28.840960999161585 60-64 28.48381470024189 20.6491466458508 22.101005136507755 28.76603351739956 65-69 29.04107278807328 22.049158210220977 21.026289668198945 27.8834793335068 70-74 28.321576223654922 22.671995913922537 20.138531517727216 28.867896344695325 75-79 28.753601819460904 22.154490348569393 20.700029874622953 28.39187795734675 80-84 28.438761841433212 20.570894407663324 21.74872551003691 29.241618240866558 85-89 28.58066630046161 21.593473840430967 20.979550338739678 28.846309520367747 90-94 28.96392880202762 21.035492979463605 21.055056039010474 28.9455221794983 95-99 29.124432623088264 21.279838484296548 20.915994487650217 28.679734404964968 100-104 27.89114072874806 21.53059257760174 21.437354843061858 29.140911850588335 105-109 27.837125483053377 21.577042797806627 21.764385594649553 28.821446124490446 110-114 29.01386231357865 21.835227883251846 20.754536969622794 28.396372833546714 115-119 27.97271772336099 22.111461254541425 20.542320776354718 29.373500245742868 120-124 28.340994728574593 22.086742413291315 20.739589657598273 28.83267320053581 125-129 29.301285235809665 22.65037181877274 19.85916517735175 28.189177768065843 130-134 29.00604238341669 22.36351633949136 20.06076112829705 28.569680148794895 135-139 28.59907292299093 22.931712394113735 19.995036957799687 28.474177725095647 140-144 28.18184008403442 23.257731263311072 20.19707614174063 28.363352510913874 145-149 27.98673952219877 22.926122948528914 20.70243911840951 28.384698410862796 150-151 28.423414958512822 23.58348511569189 19.798683589195022 28.19441633660027 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.0 2 2.0 3 2.0 4 3.0 5 2.0 6 0.5 7 1.5 8 3.0 9 3.5 10 1.5 11 1.0 12 2.0 13 2.5 14 4.5 15 7.0 16 13.5 17 19.0 18 24.5 19 40.0 20 58.5 21 78.0 22 155.0 23 203.0 24 223.5 25 290.0 26 359.5 27 465.5 28 586.0 29 738.0 30 876.5 31 992.0 32 1143.5 33 1347.5 34 1676.0 35 2025.5 36 2384.5 37 2817.0 38 3394.0 39 4058.5 40 4889.5 41 5825.0 42 6817.0 43 8260.0 44 9972.0 45 11308.0 46 13200.0 47 14994.0 48 15984.0 49 14661.0 50 12222.5 51 12017.0 52 12246.5 53 12855.5 54 13788.0 55 14498.5 56 15215.5 57 13765.5 58 12249.5 59 13118.0 60 12746.0 61 11241.5 62 11022.5 63 9348.0 64 7570.0 65 7079.5 66 7567.0 67 7640.0 68 5930.0 69 5188.5 70 5118.0 71 4934.0 72 4121.5 73 4027.5 74 5857.5 75 7528.0 76 6512.0 77 4302.0 78 3508.5 79 3317.0 80 2206.5 81 1176.0 82 1291.5 83 1611.5 84 1849.5 85 1574.0 86 1022.5 87 944.5 88 942.5 89 1052.5 90 1149.0 91 1220.5 92 1016.5 93 516.0 94 439.0 95 327.5 96 68.0 97 33.5 98 63.5 99 75.0 100 36.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 4.818487573120549E-5 115-119 0.0 120-124 0.0 125-129 4.818487573120549E-5 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 415068.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.25450287663708 #Duplication Level Percentage of deduplicated Percentage of total 1 75.61799382524191 17.58458854934613 2 7.87385259319119 3.6620505555716174 3 4.13480864466132 2.8845875856486165 4 2.4129214065187212 2.244451511559552 5 1.505356291829842 1.7503156109360394 6 0.9790514079691677 1.3660412269796756 7 0.7438718634093782 1.210885927125194 8 0.5853587783096081 1.088978191525244 9 0.46725098941174037 0.9779120529648154 >10 4.306790161828392 21.5964613027263 >50 0.7832411263753342 12.821272658937813 >100 0.550133648287437 23.76333516435861 >500 0.031080997078386276 4.681642526043926 >1k 0.007252232651623464 2.4689930324669693 >5k 0.0010360332359462092 1.8984841038094964 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT 7880 1.8984841038094964 TruSeq Adapter, Index 7 (97% over 36bp) CTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT 2692 0.6485684273420259 No Hit CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCACTCCGC 1634 0.3936704347239488 No Hit CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC 1312 0.31609278479670805 No Hit CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT 1262 0.30404656586390666 No Hit CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC 1259 0.3033237927279385 No Hit CTACGAATGGTTTAGCACCAGGTTCCCCACGAACGTGCGTTGCGTGACGG 1068 0.2573072364046373 No Hit CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG 1021 0.245983790607804 No Hit CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA 948 0.22839631096591403 No Hit CTCTTATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG 855 0.20599034375090347 No Hit CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG 831 0.2002081586631588 No Hit CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC 816 0.1965942929833184 No Hit CTCTCGCGCGCGTCACCGACTGCCAGCGACGGCCGGGTATGGGCCCGACG 779 0.1876800909730454 No Hit CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT 775 0.18671639345842128 No Hit CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG 726 0.17491109890427592 No Hit CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG 698 0.16816521630190717 No Hit ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA 683 0.16455135062206674 No Hit CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC 669 0.16117840932088237 No Hit CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT 668 0.16093748494222634 No Hit CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA 658 0.15852824115566605 No Hit GTCTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCA 654 0.15756454364104197 No Hit GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA 648 0.15611899736910578 No Hit CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT 635 0.15298698044657744 No Hit CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA 634 0.1527460560679214 No Hit CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA 629 0.15154143417464128 No Hit CTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGC 624 0.15033681228136112 No Hit CTCCCTTTCGATCGGCTGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG 605 0.1457592490868966 No Hit GGAGTCTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGC 573 0.13804966896990373 No Hit AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG 568 0.1368450470766236 No Hit CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCATGCTC 548 0.13202655950350303 No Hit CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC 543 0.1308219376102229 No Hit CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT 540 0.13009916447425482 No Hit CCCACCCCGCGGACGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGA 539 0.1298582400955988 No Hit AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG 537 0.12937639133828674 No Hit GTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAC 520 0.12528067690113426 No Hit CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT 513 0.12359420625054209 No Hit CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA 512 0.12335328187188606 No Hit CTCCCGTCCCTCTCGCGCGCGTCACCGACTGCCAGCGACGGCCGGGTATG 504 0.12142588684263783 No Hit CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT 492 0.1185347942987655 No Hit CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA 487 0.11733017240548536 No Hit CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT 487 0.11733017240548536 No Hit CTACTCTTATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCC 481 0.11588462613354919 No Hit GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG 474 0.11419815548295702 No Hit CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT 470 0.11323445796833291 No Hit CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG 468 0.11275260921102083 No Hit CTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTC 466 0.11227076045370879 No Hit AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA 465 0.11202983607505276 No Hit CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC 465 0.11202983607505276 No Hit CCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACC 458 0.11034336542446058 No Hit GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC 456 0.10986151666714852 No Hit CTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTA 448 0.1079341216379003 No Hit CTCGCGCGCGTCACCGACTGCCAGCGACGGCCGGGTATGGGCCCGACGCT 441 0.1062476509873081 No Hit CTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAAC 439 0.10576580222999604 No Hit CTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTC 429 0.10335655844343576 No Hit GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA 420 0.10118823903553154 No Hit CGACGGGCGCTGACCCCCCTCGCGGGGGGGATGCGTGCATTTATCAGATC 418 0.10070639027821948 No Hit CTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGT 416 0.1002245415209074 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.004336638815808494 0.0 0.0 0.0 0.0 2 0.004336638815808494 0.0 0.0 0.0 0.0 3 0.005300336330432604 0.0 0.0 0.0 0.0 4 0.006264033845056713 0.0 0.0 0.0 0.0 5 0.006264033845056713 0.0 0.0 0.0 0.0 6 0.006504958223712741 0.0 0.0 0.0 0.0 7 0.006504958223712741 0.0 0.0 0.0 0.0 8 0.006504958223712741 0.0 0.0 0.0 0.0 9 0.006986806981024796 0.0 0.0 0.0 0.0 10-14 0.007950504495648906 0.0 0.0 0.0 0.0 15-19 0.009251496140391453 0.0 0.0 0.0 0.0 20-24 0.012238958435726194 0.0 0.0 0.0 0.0 25-29 0.016190118245685045 0.0 0.0 0.0 0.0 30-34 0.020237647807106306 0.0 0.0 0.0 0.0 35-39 0.022791446220860195 0.0 0.0 0.0 0.0 40-44 0.02929640444457294 0.0 0.0 0.0 0.0 45-49 0.03734327869168425 0.0 0.0 0.0 0.0 50-54 0.04881127911571116 0.0 0.0 0.0 0.0 55-59 0.06254396869910472 0.0 0.0 0.0 0.0 60-64 0.08278161650621103 0.0 0.0 0.0 0.0 65-69 0.11154798731774071 0.0 0.0 0.0 0.0 70-74 0.15134869467171647 0.0 0.0 0.0 0.0 75-79 0.21625372228165024 0.0 0.0 0.0 0.0 80-84 0.3092505324428768 0.0 0.0 0.0 0.0 85-89 0.4475893106671678 0.0 0.0 0.0 0.0 90-94 0.6450027465379168 0.0 0.0 0.0 0.0 95-99 0.9183073616853141 0.0 0.0 0.0 0.0 100-104 1.3206992589166113 0.0 0.0 0.0 0.0 105-109 1.8771382038605722 0.0 0.0 0.0 0.0 110-114 2.6254493239661936 0.0 0.0 0.0 0.0 115-119 3.5721375774571875 0.0 0.0 0.0 0.0 120-124 4.767748898975588 0.0 0.0 0.0 0.0 125-129 6.218065473609144 0.0 0.0 0.0 0.0 130-134 8.189549664151418 0.0 0.0 0.0 0.0 135-139 10.195967889598814 0.0 0.0 0.0 0.0 140 11.438125801073559 0.0 0.0 0.0 0.0 >>END_MODULE