##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename DRR051423_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 209048 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.56214840610769 29.0 16.0 32.0 16.0 33.0 2 21.881113428494892 16.0 16.0 32.0 16.0 33.0 3 22.160805173931347 16.0 16.0 32.0 16.0 33.0 4 22.572681872105928 16.0 16.0 32.0 16.0 33.0 5 22.623010026405417 16.0 16.0 32.0 16.0 33.0 6 24.390125712754966 18.0 16.0 33.0 16.0 37.0 7 23.586047223604147 16.0 16.0 33.0 16.0 37.0 8 22.48786403122728 16.0 16.0 33.0 16.0 37.0 9 22.19955225594122 16.0 16.0 33.0 16.0 37.0 10-14 23.056066549309254 16.0 16.0 34.2 15.4 37.4 15-19 23.185567907848913 15.8 14.8 36.0 13.8 38.0 20-24 24.416822930618807 23.0 14.4 36.0 12.4 38.4 25-29 21.176879951016037 14.6 12.2 35.2 12.0 39.0 30-34 21.47436186904443 14.6 13.0 34.8 12.0 39.0 35-39 22.105964180475297 15.6 13.2 35.0 12.6 39.0 40-44 23.142303203092116 17.8 13.8 35.6 12.4 39.0 45-49 21.623373579273657 14.0 12.0 34.4 12.0 39.0 50-54 21.470114997512532 14.2 12.0 34.2 12.0 39.0 55-59 22.445999961731275 17.0 13.6 34.8 13.2 39.0 60-64 26.010732463357698 26.2 15.2 36.0 14.0 39.0 65-69 25.748423328613523 28.2 14.0 35.0 12.0 39.0 70-74 22.3740538058245 17.4 12.0 33.8 12.0 39.0 75-79 22.164661704488925 15.0 13.4 34.0 12.0 39.0 80-84 22.218121197045654 15.0 14.0 34.0 12.0 39.0 85-89 22.175472618728712 15.0 14.0 34.0 12.0 39.0 90-94 22.13326508744403 15.0 13.6 33.4 12.0 39.0 95-99 22.109638934598753 15.0 14.0 33.0 12.0 39.0 100-104 22.018044659599706 15.0 14.0 33.0 12.0 39.0 105-109 21.904913704029696 15.0 14.0 33.0 12.0 38.2 110-114 21.844056867322337 15.0 14.0 33.0 12.0 38.2 115-119 21.728478626918218 14.6 13.8 33.0 12.0 38.0 120-124 21.63501779495618 14.0 13.4 32.2 12.0 38.0 125-129 21.527298993532586 14.0 13.0 32.0 12.0 38.0 130-134 21.39394397458957 14.0 13.0 31.0 12.0 38.0 135-139 21.291335961118975 14.0 13.0 31.0 12.0 38.0 140-144 21.16857659484903 14.0 13.0 30.0 12.0 38.0 145-149 21.0356951513528 14.0 13.0 29.2 12.0 38.0 150-151 20.320847365198425 14.0 12.0 27.0 12.0 37.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.6136479755201911 1101 2 -0.38413420578816115 1101 3 -0.48335019834830106 1101 4 -0.30355827903055754 1101 5 -0.3921475415869793 1101 6 -0.26506515889321136 1101 7 0.07010213809508414 1101 8 -0.4165183949377571 1101 9 -0.5964667279502365 1101 10-14 -0.41799755782665216 1101 15-19 -0.3469245351843995 1101 20-24 -0.30645949001229766 1101 25-29 -0.4123230044480941 1101 30-34 -0.5689128307989648 1101 35-39 -0.5052460347667527 1101 40-44 -0.5301235924813135 1101 45-49 -0.4388328109922597 1101 50-54 -0.3759693236420887 1101 55-59 -0.4288695330192631 1101 60-64 -0.3253577639397136 1101 65-69 -0.30567347073966644 1101 70-74 -0.49547143214489253 1101 75-79 -0.2537136979709338 1101 80-84 -0.24139744981819433 1101 85-89 -0.3752927084076916 1101 90-94 -0.2835110240578764 1101 95-99 -0.41861629073454054 1101 100-104 -0.4926704409717786 1101 105-109 -0.5273288246220353 1101 110-114 -0.4689914396612771 1101 115-119 -0.37154564632835374 1101 120-124 -0.24655487468091053 1101 125-129 -0.35572085423979516 1101 130-134 -0.34639861509567993 1101 135-139 -0.4038521308246885 1101 140-144 -0.5962610752833513 1101 145-149 -0.33446196134269 1101 150-151 -0.27825982782817604 1102 1 -0.24666998799502693 1102 2 -0.21473550607528225 1102 3 -0.06172714960403525 1102 4 -0.14321703616728598 1102 5 -0.09386200645612419 1102 6 0.02977659550433387 1102 7 -0.28515181391970046 1102 8 -0.2740280480845172 1102 9 -0.2506415076604753 1102 10-14 -0.2138818520743655 1102 15-19 -0.5382185518835172 1102 20-24 -0.12602716104625955 1102 25-29 -0.27105109923044424 1102 30-34 -0.40325922200717557 1102 35-39 -0.40815715981213074 1102 40-44 -0.15101696233709916 1102 45-49 -0.3295169468293615 1102 50-54 -0.1028357636935695 1102 55-59 -0.31404658988207146 1102 60-64 0.23952887346499452 1102 65-69 0.24230217362850937 1102 70-74 -0.2286488850342323 1102 75-79 -0.19952901759735298 1102 80-84 -0.16070341331774785 1102 85-89 -0.348625271689432 1102 90-94 -0.24488292597530759 1102 95-99 -0.15726518021013547 1102 100-104 -0.17560928787451857 1102 105-109 -0.24277715966048063 1102 110-114 -0.17265303498816564 1102 115-119 0.02507723740485801 1102 120-124 0.07266891331288505 1102 125-129 -0.055995762658657355 1102 130-134 0.033334727038244694 1102 135-139 0.04453439107227908 1102 140-144 -0.04219223267089589 1102 145-149 -0.08451839512375514 1102 150-151 -0.19576832589755 1103 1 0.4256078792690623 1103 2 -0.14380184132462048 1103 3 0.004634264261529353 1103 4 -0.40841903448294303 1103 5 -0.2785819233837543 1103 6 -0.21607183012709186 1103 7 0.4259705624733563 1103 8 0.14880050068370565 1103 9 -0.05866584066948022 1103 10-14 -0.22172859336443906 1103 15-19 -0.17427326844811475 1103 20-24 0.0828439214292338 1103 25-29 -0.3026074796953573 1103 30-34 -0.2097673207455557 1103 35-39 -0.10948190245948908 1103 40-44 0.00351824939115275 1103 45-49 -0.17165687798395268 1103 50-54 -0.18743679555705484 1103 55-59 -0.17756588213164548 1103 60-64 0.15970711929706738 1103 65-69 -0.04422129676878228 1103 70-74 -0.011518642930607115 1103 75-79 -0.30761467371371864 1103 80-84 -0.2143397100561728 1103 85-89 -0.32130460089640067 1103 90-94 -0.14842053339295092 1103 95-99 -0.22589146084102296 1103 100-104 -0.32353337804401505 1103 105-109 -0.5055245983986971 1103 110-114 -0.3248046584152249 1103 115-119 -0.08259109452485802 1103 120-124 -0.39057238084997437 1103 125-129 -0.19006573641236812 1103 130-134 -0.43714269379362136 1103 135-139 -0.32024554073642975 1103 140-144 -0.10195789663662325 1103 145-149 -0.2571202119230165 1103 150-151 -0.17701653730691547 1104 1 -0.07204248210715036 1104 2 0.09731137031575443 1104 3 0.08791472189055582 1104 4 0.06669078115075777 1104 5 0.26949962644799186 1104 6 0.3387625860027974 1104 7 0.4573452927257087 1104 8 0.22602132104278283 1104 9 0.462383039317082 1104 10-14 0.3737982036075387 1104 15-19 0.2936690483722302 1104 20-24 0.6668950866248942 1104 25-29 0.36629850703053535 1104 30-34 0.3429795788895582 1104 35-39 0.40960310046838444 1104 40-44 0.4755044565801825 1104 45-49 0.3214051227892014 1104 50-54 0.40046970171162144 1104 55-59 0.36646231679408814 1104 60-64 0.6045357123031216 1104 65-69 0.3186265207015673 1104 70-74 0.5346952663428404 1104 75-79 0.3560824716507014 1104 80-84 0.41751881250149836 1104 85-89 0.46929814951015913 1104 90-94 0.3144799694835392 1104 95-99 0.4105704286892582 1104 100-104 0.2762470722449386 1104 105-109 0.8047790685381919 1104 110-114 0.46831524737087094 1104 115-119 0.4780520915231321 1104 120-124 0.4874359476851282 1104 125-129 0.5671471892914148 1104 130-134 0.5706925884647696 1104 135-139 0.5848832122831915 1104 140-144 0.5111146085952143 1104 145-149 0.6944976215160708 1104 150-151 0.5860581699465435 1105 1 0.35303391094014813 1105 2 -0.42237393739041096 1105 3 -0.044828238832952394 1105 4 -0.08216652815872649 1105 5 0.38383198448187983 1105 6 0.053822605054406836 1105 7 0.11287472129111009 1105 8 -0.09080819304388399 1105 9 -0.23039925794315508 1105 10-14 -0.18719501148899553 1105 15-19 -0.0744836111444016 1105 20-24 -0.04535003322658682 1105 25-29 -0.06133735737525825 1105 30-34 -0.09609438970287698 1105 35-39 -0.024731010183476343 1105 40-44 -0.0663073284774498 1105 45-49 0.05174018115300427 1105 50-54 -0.023016352804535956 1105 55-59 -0.0700625663241361 1105 60-64 0.007523276829484615 1105 65-69 0.18832099719620388 1105 70-74 -0.11032429644388841 1105 75-79 -0.16104401381474176 1105 80-84 0.1544167760567774 1105 85-89 -0.08847933616462456 1105 90-94 0.12239855713309922 1105 95-99 -0.024553333727148186 1105 100-104 0.07086458771422599 1105 105-109 -0.0170131131628537 1105 110-114 0.03776581786424771 1105 115-119 0.02725959986835491 1105 120-124 -0.011139076535076953 1105 125-129 0.12917217707989082 1105 130-134 0.2035765700233334 1105 135-139 0.012791706151467963 1105 140-144 0.02900510567337733 1105 145-149 0.1730989674617618 1105 150-151 -0.05885574892347378 1106 1 0.3078017641154922 1106 2 -0.25094796910629924 1106 3 -0.2677633572840179 1106 4 -0.09768241087930463 1106 5 -0.31443868271546194 1106 6 -0.2302937153141258 1106 7 0.022002830022170627 1106 8 -0.05403809248715419 1106 9 0.06732123238878884 1106 10-14 -0.13412036677825512 1106 15-19 -0.23775698766710107 1106 20-24 0.17460946275897626 1106 25-29 -0.33042659248236106 1106 30-34 -0.22349757727610609 1106 35-39 -0.28194780576287926 1106 40-44 -0.03298630299380534 1106 45-49 -0.30932961074843135 1106 50-54 -0.4611463908309936 1106 55-59 -0.3336666563664501 1106 60-64 -0.23028004373245636 1106 65-69 -0.22869804160869123 1106 70-74 -0.07362644456325995 1106 75-79 -0.017972454058533316 1106 80-84 -0.12187207159281854 1106 85-89 -0.21177905468250202 1106 90-94 -0.4211905716789346 1106 95-99 -0.2353548304895483 1106 100-104 -0.42307275733873695 1106 105-109 -0.22426994921430676 1106 110-114 -0.1292309229532158 1106 115-119 -0.20265934415011344 1106 120-124 -0.2227608834872541 1106 125-129 -0.2728987385122359 1106 130-134 -0.21083991801966917 1106 135-139 -0.2618715462857253 1106 140-144 -0.33259600171340864 1106 145-149 -0.3598635319588084 1106 150-151 -0.231335077330467 1107 1 -0.05753213540176105 1107 2 -0.6270957686516496 1107 3 -0.527140512401381 1107 4 -0.534286839884949 1107 5 -0.37636558113440444 1107 6 -0.2975014494716177 1107 7 -0.25412198160460875 1107 8 -0.5403155822064463 1107 9 -0.572165945461073 1107 10-14 -0.6355044310422535 1107 15-19 -0.6802437696139201 1107 20-24 -0.45933698816181945 1107 25-29 -0.6977055253320934 1107 30-34 -0.7389033140388932 1107 35-39 -0.7031682088177185 1107 40-44 -0.5653488224696837 1107 45-49 -0.7878560213126562 1107 50-54 -0.6740739022610995 1107 55-59 -0.7876183434242527 1107 60-64 -0.16778004373245636 1107 65-69 -0.5664141918985983 1107 70-74 -0.6182865426450483 1107 75-79 -0.7768671821625368 1107 80-84 -0.6004106146696238 1107 85-89 -0.7100447439369262 1107 90-94 -0.6340529506730839 1107 95-99 -0.3608918538095125 1107 100-104 -0.6420703563783547 1107 105-109 -0.5998095267474461 1107 110-114 -0.4728073960956607 1107 115-119 -0.5326339935843549 1107 120-124 -0.7392071428420621 1107 125-129 -0.46097886417952694 1107 130-134 -0.6563978220323392 1107 135-139 -0.7430003807982537 1107 140-144 -0.737888969964537 1107 145-149 -0.48227707184284796 1107 150-151 -0.5145167010437852 1108 1 0.11616079537879997 1108 2 -0.16913500702741047 1108 3 -0.2730115412252836 1108 4 -0.2751610246103873 1108 5 -0.6149254380668694 1108 6 0.0036504545889748385 1108 7 -0.03650942111649513 1108 8 -0.014910574842676994 1108 9 -0.5401729936411712 1108 10-14 -0.15430571444221286 1108 15-19 -0.19070558320533593 1108 20-24 -0.06370002203516023 1108 25-29 -0.24485732419596928 1108 30-34 -0.3186003474914507 1108 35-39 -0.27207205901759224 1108 40-44 0.011450041405762335 1108 45-49 -0.20740420140943527 1108 50-54 -0.25471244633852663 1108 55-59 -0.22580212209035722 1108 60-64 0.1570910902881586 1108 65-69 0.2241286231655728 1108 70-74 -0.26148820475318857 1108 75-79 -0.24836676137566727 1108 80-84 -0.30190703798121277 1108 85-89 -0.23313016367183081 1108 90-94 -0.3430577897221063 1108 95-99 -0.3626644185054957 1108 100-104 -0.24487384890938557 1108 105-109 -0.34022610575017964 1108 110-114 -0.3431287720687344 1108 115-119 -0.2586991103527083 1108 120-124 -0.1154721333743538 1108 125-129 -0.29821324246691816 1108 130-134 -0.3021715438922179 1108 135-139 -0.3014003387195672 1108 140-144 -0.24893988517598586 1108 145-149 -0.39712821848707236 1108 150-151 -0.1121195308354963 1109 1 0.4997104316758332 1109 2 -0.24535563605038746 1109 3 -0.11800604643225299 1109 4 -0.22088686060389762 1109 5 -0.1503995602082675 1109 6 -0.2103782753288641 1109 7 -0.4615251205251525 1109 8 -0.056631753344952074 1109 9 -0.4013705968647834 1109 10-14 -0.2800540529214608 1109 15-19 -0.5805493740892125 1109 20-24 -0.21188412376733012 1109 25-29 -0.4407323045717071 1109 30-34 -0.6242809168566694 1109 35-39 -0.407058191206616 1109 40-44 -0.2579858265098771 1109 45-49 -0.44371300877769215 1109 50-54 -0.59858182725851 1109 55-59 -0.38328419859880825 1109 60-64 0.09180117454165426 1109 65-69 -0.09513324297079961 1109 70-74 -0.3709994298303769 1109 75-79 -0.5887430238697817 1109 80-84 -0.45639968542482734 1109 85-89 -0.46073364894055047 1109 90-94 -0.6327916847453956 1109 95-99 -0.37283632703026726 1109 100-104 -0.4559183828986093 1109 105-109 -0.2961526325176713 1109 110-114 -0.37802986579420406 1109 115-119 -0.3554597990334365 1109 120-124 -0.23503097999113365 1109 125-129 -0.3360462007588261 1109 130-134 -0.270661522436356 1109 135-139 -0.244448117253242 1109 140-144 -0.05325983912438659 1109 145-149 -0.40009148187082033 1109 150-151 -0.15808282038492294 1110 1 0.4423305019608783 1110 2 0.08724881533579065 1110 3 0.06081082197852439 1110 4 0.11063728361060043 1110 5 0.2132995156735511 1110 6 0.5914048822918545 1110 7 0.542587856176656 1110 8 0.39155254966168584 1110 9 0.43221003570062067 1110 10-14 0.23309185362545648 1110 15-19 0.01929573849701427 1110 20-24 0.29195422062939613 1110 25-29 0.1342240926689513 1110 30-34 0.03940123855137401 1110 35-39 0.20947961954439265 1110 40-44 0.0961870226375865 1110 45-49 0.01934308910074023 1110 50-54 0.023505898890128663 1110 55-59 0.06005434103414942 1110 60-64 0.6827968793444619 1110 65-69 0.534394285338454 1110 70-74 0.009069553639616146 1110 75-79 -0.03307466515573765 1110 80-84 -0.20615498934256848 1110 85-89 -0.10319492708710953 1110 90-94 -0.1960963834308025 1110 95-99 -0.2747223090685367 1110 100-104 -0.0970546577912259 1110 105-109 0.08547043138965549 1110 110-114 -0.1280441831181669 1110 115-119 0.14145156371549206 1110 120-124 -0.13060562531197206 1110 125-129 0.04690967585187522 1110 130-134 0.053140377863002186 1110 135-139 -0.19897369320014135 1110 140-144 -0.14466814317228582 1110 145-149 -0.09992097555818091 1110 150-151 -0.0483242625299205 1111 1 0.017846929267584244 1111 2 -0.17365026001341022 1111 3 0.014165140294480238 1111 4 -0.3219845276557116 1111 5 -0.042005750245525775 1111 6 0.1605208898989119 1111 7 -0.12916438987226542 1111 8 -0.3728877415049503 1111 9 -0.31870477838554834 1111 10-14 -0.25129400774250144 1111 15-19 -0.4398084650573182 1111 20-24 -0.1607390707339782 1111 25-29 -0.25454369538272203 1111 30-34 -0.21097649113581696 1111 35-39 -0.33842057063201025 1111 40-44 -0.19412250585608248 1111 45-49 -0.25562117085073055 1111 50-54 -0.5114701651140656 1111 55-59 -0.203612784679013 1111 60-64 0.0973414396435004 1111 65-69 0.30508875710709304 1111 70-74 -0.13558976828313263 1111 75-79 -0.20349239746605008 1111 80-84 -0.13332660715357747 1111 85-89 -0.15381529563586582 1111 90-94 -0.15527665754713738 1111 95-99 -0.06663850029419649 1111 100-104 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0.6622082440926889 2113 3 0.6127852891428631 2113 4 0.8577594300998364 2113 5 0.5661557311879939 2113 6 0.514498033866019 2113 7 0.9092599323424047 2113 8 1.0298572126498904 2113 9 1.1142504987537407 2113 10-14 0.882242562269834 2113 15-19 1.044713892775924 2113 20-24 0.7753751878478248 2113 25-29 1.069338233099547 2113 30-34 1.1737764414075897 2113 35-39 1.0397176375039336 2113 40-44 0.7597042789396546 2113 45-49 1.0533244804239068 2113 50-54 1.2021546060198496 2113 55-59 0.982523236414437 2113 60-64 0.6906763557049587 2113 65-69 0.6295192447672129 2113 70-74 0.7873590700304014 2113 75-79 0.845687641454667 2113 80-84 0.8603190991568361 2113 85-89 0.8571242333586326 2113 90-94 0.6725739616952389 2113 95-99 0.8112227304208091 2113 100-104 0.8238205991599798 2113 105-109 0.9420473003715877 2113 110-114 0.9272690268785908 2113 115-119 0.7865754412444304 2113 120-124 0.6375053776091555 2113 125-129 0.6732593458724345 2113 130-134 0.6652284445080348 2113 135-139 0.8830472956562936 2113 140-144 0.8360891866989668 2113 145-149 0.7821941385822484 2113 150-151 0.5144908407915132 2114 1 -0.2513137970025703 2114 2 0.007625247414352998 2114 3 -0.027296106553936994 2114 4 -0.31612212719136323 2114 5 -0.15857718918188013 2114 6 -0.6296262142711555 2114 7 -0.20119283122105003 2114 8 -0.4634665447191111 2114 9 -0.08895744929665028 2114 10-14 -0.16220547316123302 2114 15-19 -0.1748450143671434 2114 20-24 -0.28800213507164685 2114 25-29 -0.23497394333759658 2114 30-34 -0.049780430843064494 2114 35-39 -0.2672961578696835 2114 40-44 -0.8013634410830868 2114 45-49 -0.17834921859156694 2114 50-54 -0.08504292816933301 2114 55-59 0.10635003115962505 2114 60-64 -0.3990743699735937 2114 65-69 -0.11158498361954017 2114 70-74 -0.17882460893539687 2114 75-79 -0.24186899139687057 2114 80-84 -0.12841358181393048 2114 85-89 -0.022993072204407383 2114 90-94 -0.04820767639315804 2114 95-99 -0.18061974496597344 2114 100-104 -0.07155002058446058 2114 105-109 -0.09365668263107452 2114 110-114 -0.2295062671334378 2114 115-119 -0.013005610316156435 2114 120-124 -0.26643213922359266 2114 125-129 -0.2845633192381385 2114 130-134 -0.4492546030426148 2114 135-139 -0.1883463064025399 2114 140-144 -0.36573742849688884 2114 145-149 -0.3021413792679475 2114 150-151 -0.5247017857159513 >>END_MODULE >>Per sequence quality scores fail #Quality Count 7 1.0 8 1.0 9 0.0 10 0.0 11 0.0 12 0.0 13 9.0 14 834.0 15 25683.0 16 59564.0 17 27856.0 18 14427.0 19 8580.0 20 5129.0 21 3281.0 22 2354.0 23 1826.0 24 1656.0 25 1506.0 26 1431.0 27 1414.0 28 1411.0 29 1473.0 30 1690.0 31 1783.0 32 2089.0 33 2550.0 34 3167.0 35 4198.0 36 6459.0 37 13984.0 38 14692.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 76.96892579694615 6.454498488385442 4.401859859936474 12.174715854731929 2 47.55367188396924 25.843825341548353 13.72459913512686 12.877903639355553 3 16.55026596762466 32.31506639623436 35.190960927633846 15.943706708507138 4 15.499789522023649 26.53840266350312 30.42411312234511 27.537694692128124 5 38.84084038115648 17.18074317860013 18.926275305193066 25.05214113505032 6 64.07810646358732 11.416038421797865 10.125425739543072 14.380429375071754 7 58.78602043549807 16.196280280127052 12.467949944510352 12.54974933986453 8 27.68024568520148 47.96314721977728 10.98455857028051 13.372048524740729 9 36.002430075533255 41.25005381564911 10.751170789344023 11.996345319473612 10-14 51.020292966489 26.242801885501787 9.30016962627686 13.436735521732352 15-19 54.66548798887144 9.860519938502103 15.420779362423332 20.053212710203123 20-24 66.58668495913375 10.622355269943046 12.750193974775147 10.040765796148058 25-29 38.433506595142354 43.695652381895435 8.092694589467097 9.778146433495115 30-34 57.6568060923807 19.95446021966247 12.480100264054188 9.908633423902645 35-39 23.13721850908597 30.34026700139107 28.53436250882574 17.98815198069722 40-44 59.89244493088616 9.549650221194913 13.709874936856123 16.848029911062806 45-49 55.35384730190894 8.714179245129582 12.732016313972212 23.199957138989262 50-54 39.1180139911522 14.900424020695885 20.095538598129412 25.8860233900225 55-59 13.09855247170479 27.477492991973058 40.879997703854656 18.543956832467494 60-64 9.58536359017976 71.11331583796469 9.18497286240623 10.116347709449316 65-69 10.602109017571319 69.59691014192147 8.331116906774229 11.469863933732992 70-74 17.61883531631376 51.77176654280215 13.3102060029008 17.29919213798329 75-79 22.753813286491834 37.80757672735205 17.49667775516706 21.94193223098906 80-84 26.23803250394412 30.526287591116567 19.502653490581473 23.733026414357838 85-89 27.371314419329103 28.135171284186516 19.782304620453836 24.711209676030546 90-94 26.79698666886078 27.564574798463866 20.386960856162613 25.25147767651274 95-99 25.43062704502401 28.568626700598916 20.95597099175293 25.044775262624135 100-104 23.888199506514905 31.334677230322068 20.127073847681682 24.650049415481348 105-109 24.148362740164288 31.26675481380989 20.210347658200842 24.374534787824977 110-114 24.540981363714206 29.964629422159376 20.263887281038713 25.230501933087705 115-119 24.938769105952407 28.92643108080182 20.584295806449145 25.550504006796633 120-124 25.00980652913927 28.64769398271372 20.664318202257512 25.678181285889508 125-129 25.251980203229746 28.544202613609944 20.70432951500209 25.499487668158217 130-134 25.54765615507445 28.27854619140148 20.43556088773061 25.73823676579346 135-139 25.18842814855352 28.12004293808229 20.957668485093176 25.733860428271015 140-144 25.5628458588781 28.03102492862774 20.894564227380926 25.51156498511324 145-149 25.413762587002797 28.044966394795377 21.047621325551916 25.493649692649907 150-151 25.22704387322377 29.135044762982222 20.1817307715306 25.456180592263404 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.5 8 3.5 9 3.5 10 0.5 11 1.0 12 2.5 13 1.5 14 0.0 15 2.5 16 6.5 17 8.5 18 10.0 19 13.5 20 20.5 21 21.5 22 39.5 23 68.0 24 86.5 25 111.5 26 130.0 27 147.5 28 211.5 29 267.0 30 286.0 31 321.0 32 392.5 33 466.0 34 570.0 35 712.0 36 892.0 37 1145.5 38 1404.5 39 1806.5 40 2280.0 41 2894.5 42 4090.0 43 4856.5 44 5377.0 45 6443.5 46 7470.0 47 8598.0 48 9552.0 49 10003.5 50 10176.0 51 10495.0 52 10546.0 53 10664.5 54 10268.5 55 9313.5 56 8805.5 57 7922.0 58 7011.0 59 6438.0 60 5791.0 61 5069.5 62 4359.0 63 3635.5 64 3083.0 65 2783.5 66 2664.0 67 2399.0 68 1950.0 69 1628.5 70 1379.5 71 1123.0 72 969.5 73 919.0 74 939.0 75 914.0 76 736.0 77 588.0 78 552.0 79 535.0 80 462.5 81 364.5 82 332.5 83 331.0 84 354.0 85 359.0 86 315.5 87 287.5 88 271.0 89 245.5 90 244.0 91 235.5 92 201.0 93 171.5 94 149.5 95 110.0 96 61.5 97 39.5 98 42.0 99 51.0 100 34.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 4.7835903715893005E-4 10-14 9.5671807431786E-5 15-19 9.5671807431786E-5 20-24 9.5671807431786E-5 25-29 9.5671807431786E-5 30-34 0.0 35-39 1.91343614863572E-4 40-44 7.65374459454288E-4 45-49 9.5671807431786E-5 50-54 7.65374459454288E-4 55-59 9.567180743178601E-4 60-64 0.001052389881749646 65-69 0.0013394053040450041 70-74 0.0011480616891814321 75-79 0.001243733496613218 80-84 0.001243733496613218 85-89 0.001722092533772148 90-94 0.001722092533772148 95-99 0.0019134361486357202 100-104 0.002009107956067506 105-109 0.001722092533772148 110-114 0.002009107956067506 115-119 0.001530748918908576 120-124 0.001722092533772148 125-129 0.0018177643412039342 130-134 0.001243733496613218 135-139 0.0013394053040450041 140-144 0.001530748918908576 145-149 0.00143507711147679 150-151 0.001195897592897325 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 209048.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.40675665578765 #Duplication Level Percentage of deduplicated Percentage of total 1 94.8766596130159 82.92861099108877 2 2.7483482088902464 4.804484061996792 3 0.9485592912576137 2.487314734336219 4 0.49073431572271875 1.715739796680806 5 0.26668521886985236 1.1655045014726326 6 0.153163994931032 0.8032540820019005 7 0.12268957216794989 0.7506728305080685 8 0.08138545970258922 0.5690911257234903 9 0.06327817009407845 0.4977845653532989 >10 0.2435923340576473 3.3126560515119428 >50 0.0032692141935886905 0.17240335038304108 >100 5.448690322647818E-4 0.05095900135631325 >500 0.0010897380645295636 0.7415249075867264 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA 908 0.4343500057403084 No Hit TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT 649 0.3104550151161456 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.00334851326011251 0.0 0.0 0.0 0.0 2 0.00334851326011251 0.0 0.0 0.0 0.0 3 0.00382687229727144 0.0 0.0 0.0 0.0 4 0.00382687229727144 0.0 0.0 0.0 0.0 5 0.00382687229727144 0.0 0.0 0.0 0.0 6 0.00382687229727144 0.0 0.0 0.0 0.0 7 0.0047835903715893 0.0 0.0 0.0 0.0 8 0.0047835903715893 0.0 0.0 0.0 0.0 9 0.0047835903715893 0.0 0.0 0.0 0.0 10-14 0.005548964831043587 0.0 0.0 0.0 0.0 15-19 0.006410011097929662 0.0 0.0 0.0 0.0 20-24 0.008323447246565382 0.0 0.0 0.0 0.0 25-29 0.00861046266886074 0.0 0.0 0.0 0.0 30-34 0.010236883395201101 2.87015422295358E-4 0.0 0.0 0.0 35-39 0.011671960506677893 4.7835903715893E-4 0.0 0.0 0.0 40-44 0.014350771114767898 4.7835903715893E-4 0.0 0.0 0.0 45-49 0.018751674256630053 4.7835903715893E-4 0.0 0.0 0.0 50-54 0.025927059814014008 4.7835903715893E-4 0.0 0.0 0.0 55-59 0.03712066128353296 4.7835903715893E-4 0.0 0.0 0.0 60-64 0.05424591481382266 4.7835903715893E-4 0.0 0.0 0.0 65-69 0.07424132256706593 4.7835903715893E-4 0.0 0.0 0.0 70-74 0.09471508935746815 4.7835903715893E-4 0.0 0.0 0.0 75-79 0.12332095977957216 4.7835903715893E-4 0.0 0.0 0.0 80-84 0.17039148903601087 4.7835903715893E-4 0.0 0.0 0.0 85-89 0.2282729325322414 4.7835903715893E-4 0.0 0.0 0.0 90-94 0.30423634763307944 4.7835903715893E-4 4.7835903715893E-4 0.0 0.0 95-99 0.40220427844322837 4.7835903715893E-4 8.610462668860741E-4 0.0 0.0 100-104 0.5281083770234587 5.74030844590716E-4 9.5671807431786E-4 0.0 0.0 105-109 0.6969691171405611 9.5671807431786E-4 9.5671807431786E-4 0.0 0.0 110-114 0.9230415981018714 9.5671807431786E-4 9.5671807431786E-4 0.0 0.0 115-119 1.1765718877961042 9.5671807431786E-4 9.5671807431786E-4 0.0 0.0 120-124 1.476598675902185 9.5671807431786E-4 9.5671807431786E-4 0.0 0.0 125-129 1.8633997933488957 9.5671807431786E-4 9.5671807431786E-4 0.0 0.0 130-134 2.34893421606521 9.5671807431786E-4 9.5671807431786E-4 0.0 0.0 135-139 2.8961769545750258 9.5671807431786E-4 9.5671807431786E-4 0.0 0.0 140 3.2590601201637903 9.5671807431786E-4 9.5671807431786E-4 0.0 0.0 >>END_MODULE