##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	DRR051423_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	209048
Sequences flagged as poor quality	0
Sequence length	151
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40656212927175	33.0	32.0	34.0	32.0	34.0
2	32.28277716122613	33.0	32.0	34.0	32.0	34.0
3	32.361323715127625	33.0	32.0	34.0	32.0	34.0
4	32.59892464888447	33.0	32.0	34.0	32.0	34.0
5	32.43279055527917	33.0	32.0	34.0	32.0	34.0
6	32.91511518885615	33.0	32.0	34.0	31.0	37.0
7	32.72481439669358	33.0	32.0	34.0	31.0	37.0
8	32.94583540622249	33.0	32.0	34.0	32.0	37.0
9	32.706952470246065	33.0	32.0	34.0	31.0	37.0
10-14	35.67776395851671	37.4	35.8	37.4	32.4	37.4
15-19	36.475287015422296	38.0	37.2	38.0	33.8	38.0
20-24	36.82943056140216	38.4	37.4	38.4	34.4	38.4
25-29	37.03699437449772	39.0	37.2	39.0	34.2	39.0
30-34	36.82511767632314	38.8	37.0	38.8	33.8	39.0
35-39	35.813836056790784	38.2	36.6	38.4	31.6	39.0
40-44	35.12808541578968	37.8	35.4	39.0	24.8	39.0
45-49	35.244146798821326	37.6	36.4	38.6	27.2	39.0
50-54	34.871825609429415	37.4	35.2	38.4	23.2	39.0
55-59	35.13239638743255	38.0	36.0	38.8	21.6	39.0
60-64	34.782080670468034	37.8	35.4	39.0	21.2	39.0
65-69	33.75057116069037	37.8	32.4	38.8	17.8	39.0
70-74	27.685344992537598	31.6	14.8	37.4	14.2	39.0
75-79	25.21851632161035	21.2	15.8	37.0	14.0	39.0
80-84	25.47372852167923	19.0	16.0	37.0	14.0	39.0
85-89	25.5408097661781	19.0	16.0	37.0	14.0	39.0
90-94	25.457435612873603	19.0	16.0	37.0	14.0	39.0
95-99	25.28906471241055	19.0	16.0	37.0	14.0	39.0
100-104	25.127345872718227	19.0	16.0	37.0	14.0	39.0
105-109	24.92488902070338	19.0	15.6	37.0	14.0	39.0
110-114	24.779932838391183	18.8	15.0	37.0	14.0	39.0
115-119	24.52509280165321	18.0	15.0	37.0	14.0	39.0
120-124	24.316142704067964	18.0	15.0	37.0	13.0	39.0
125-129	24.0941774137997	17.0	15.0	37.0	13.0	39.0
130-134	23.918422563239062	17.0	15.0	37.0	13.0	39.0
135-139	23.72082296888753	17.0	14.6	36.4	13.0	39.0
140-144	23.512744441467987	16.0	14.0	35.8	13.0	39.0
145-149	23.320393402472156	16.0	14.0	35.0	13.0	38.2
150-151	22.82160556427232	16.0	14.0	34.0	13.0	38.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.035416576168202596
1101	2	-0.5447390517412387
1101	3	-0.06995056835615543
1101	4	0.08089230126590508
1101	5	0.22612372371450817
1101	6	0.17596016754099253
1101	7	0.1081387136113392
1101	8	0.03643182792738031
1101	9	0.11129881198566238
1101	10-14	0.22866859181348076
1101	15-19	0.17011610968333457
1101	20-24	0.16431764794336345
1101	25-29	0.13740406735861654
1101	30-34	0.20195772510537324
1101	35-39	0.23445857209350152
1101	40-44	0.0859389864729323
1101	45-49	0.18461018518401318
1101	50-54	0.018740333617266458
1101	55-59	-0.05248440841641866
1101	60-64	0.07519082630830809
1101	65-69	0.03256713792811894
1101	70-74	-0.4524543294683241
1101	75-79	-0.44998296530646087
1101	80-84	-0.27736740885487876
1101	85-89	-0.37985158874427327
1101	90-94	-0.5352052603655686
1101	95-99	-0.38910197383974676
1101	100-104	-0.45945333934181676
1101	105-109	-0.35146487861678466
1101	110-114	-0.47708721021335876
1101	115-119	-0.4102288172346249
1101	120-124	-0.23285863211146918
1101	125-129	-0.24620389096539697
1101	130-134	-0.3997529000434241
1101	135-139	-0.3552534835504062
1101	140-144	-0.5470472495014391
1101	145-149	-0.34031186412623526
1101	150-151	-0.5705874352470239
1102	1	0.13581869659507362
1102	2	0.2609967325563076
1102	3	0.1978128717943335
1102	4	0.07619278639840843
1102	5	0.08917609822359651
1102	6	0.08077023981583409
1102	7	0.17496489096522083
1102	8	0.21055974449779313
1102	9	-0.03066349948920788
1102	10-14	0.02630299331837449
1102	15-19	-0.07640875748275988
1102	20-24	0.2086043051493931
1102	25-29	0.06163759100470401
1102	30-34	-0.01590769187755825
1102	35-39	0.10567338348587185
1102	40-44	0.2508886581009264
1102	45-49	-1.326020487071034
1102	50-54	-0.01980063862787773
1102	55-59	0.11794924063806889
1102	60-64	0.10319386251173057
1102	65-69	0.029909799886468136
1102	70-74	-0.38529449975973407
1102	75-79	-0.10668571439064678
1102	80-84	-0.0780073217290429
1102	85-89	0.2813206497957026
1102	90-94	0.13548316575898056
1102	95-99	0.10931094982613487
1102	100-104	0.2607555646547546
1102	105-109	0.14462897967171884
1102	110-114	0.27528696885663706
1102	115-119	0.3797226695149867
1102	120-124	0.21494804093562792
1102	125-129	0.3236291178461954
1102	130-134	0.24959332615372887
1102	135-139	0.29709429375067486
1102	140-144	0.22409957733595576
1102	145-149	0.4213428667407335
1102	150-151	0.009567510134317558
1103	1	0.14883718346784036
1103	2	0.26127636592589454
1103	3	0.10328534589554295
1103	4	0.10954601567324573
1103	5	0.13552164984539417
1103	6	0.24221190518790792
1103	7	0.33191770487820804
1103	8	0.21534294687221944
1103	9	0.0244929561987135
1103	10-14	0.1399751027514995
1103	15-19	0.2167385896796148
1103	20-24	0.005766762325428942
1103	25-29	0.14815386615739357
1103	30-34	0.2532690249650358
1103	35-39	0.25581982306626116
1103	40-44	0.12770835998885133
1103	45-49	0.014755781704060666
1103	50-54	-0.17387175707958846
1103	55-59	0.008198212453976339
1103	60-64	0.021243624169251518
1103	65-69	0.4363982752304878
1103	70-74	-0.08140236301737858
1103	75-79	-0.056834116310469085
1103	80-84	-0.07884033481948194
1103	85-89	-0.41886201130009937
1103	90-94	-0.23896423127952104
1103	95-99	-0.06873276128479233
1103	100-104	0.054198080373911495
1103	105-109	-0.1352670163381937
1103	110-114	-0.2828049830987531
1103	115-119	-0.12155661226877967
1103	120-124	-0.13220713923166372
1103	125-129	-0.10548774764361113
1103	130-134	-0.0847575891958563
1103	135-139	-0.08136674154690482
1103	140-144	-0.23897459091829631
1103	145-149	-0.15193497599411288
1103	150-151	-0.4485422664042815
1104	1	0.17514889512371212
1104	2	0.22659697211797436
1104	3	0.2815218691154513
1104	4	0.20030029778973102
1104	5	0.23779821397396717
1104	6	0.1215313337062085
1104	7	0.2629503877724133
1104	8	0.23693580775501744
1104	9	0.3323323162749219
1104	10-14	0.15025446589422842
1104	15-19	-0.05911175766891574
1104	20-24	-0.3881722955029261
1104	25-29	0.055285969512141264
1104	30-34	-0.2276352066680687
1104	35-39	-0.11616654723548692
1104	40-44	0.06594899364912266
1104	45-49	-0.09623002259657198
1104	50-54	-0.05867340386553366
1104	55-59	0.18194587382545535
1104	60-64	0.013841569275307108
1104	65-69	0.0791031407195959
1104	70-74	0.047517031573473645
1104	75-79	0.3227787519776477
1104	80-84	0.2038431302817365
1104	85-89	0.3751970974846621
1104	90-94	0.27520977409993463
1104	95-99	0.2320115220224146
1104	100-104	0.22158075747761785
1104	105-109	0.028762389621618922
1104	110-114	0.15159092081481162
1104	115-119	0.329530615516493
1104	120-124	0.24884634053339028
1104	125-129	0.2193013068409222
1104	130-134	0.40734824485653576
1104	135-139	0.40451536384674114
1104	140-144	0.33504188020341275
1104	145-149	0.2785997237887834
1104	150-151	0.3530099141680161
1105	1	0.01582780739706635
1105	2	-0.1041723460324917
1105	3	0.03321647218805168
1105	4	-0.08814005072159858
1105	5	0.04579729222673734
1105	6	0.11132071121849663
1105	7	-0.1548493950976919
1105	8	-0.057330372722439904
1105	9	0.34905845835706373
1105	10-14	-0.1465575544685933
1105	15-19	-0.2731044648307943
1105	20-24	-0.0257977577964823
1105	25-29	-0.1708033718118429
1105	30-34	-0.0010551323116345657
1105	35-39	-0.10021675000365349
1105	40-44	-0.15377607252031567
1105	45-49	0.1349691737956391
1105	50-54	-0.0435645387019008
1105	55-59	-0.1887753446984135
1105	60-64	-0.0969079832391273
1105	65-69	-0.45401760654478096
1105	70-74	-0.3046082611380676
1105	75-79	-0.1523926556829167
1105	80-84	-0.04609966621497463
1105	85-89	0.07559808998573203
1105	90-94	0.24229690680069993
1105	95-99	0.09249696474507374
1105	100-104	0.1077933338098056
1105	105-109	0.10502106954326962
1105	110-114	-0.028737565231590878
1105	115-119	0.04115452966417976
1105	120-124	0.11574247033097507
1105	125-129	-0.04430704146268383
1105	130-134	-0.184574133640794
1105	135-139	-0.053531992540008844
1105	140-144	-0.16735839502252148
1105	145-149	-0.18639806109683477
1105	150-151	-0.08722581294936305
1106	1	0.10661977756412
1106	2	-0.043769575647417014
1106	3	0.01777891214732108
1106	4	-0.02179434720011386
1106	5	0.10139041070018351
1106	6	-0.02867548453705382
1106	7	0.12078729636049701
1106	8	-0.18079728618546653
1106	9	0.17552417140514365
1106	10-14	0.22110732676065226
1106	15-19	0.039347402685599775
1106	20-24	0.021317330188658445
1106	25-29	-0.1381009356568086
1106	30-34	0.1733202443165638
1106	35-39	-0.003858754616693716
1106	40-44	0.21330090908344346
1106	45-49	0.4107951184231027
1106	50-54	0.23357850568467597
1106	55-59	0.05081213454207756
1106	60-64	0.5174094026375329
1106	65-69	0.18761457416641747
1106	70-74	0.10301429387562067
1106	75-79	0.20685589414868844
1106	80-84	0.08759235302363422
1106	85-89	0.028461263460876296
1106	90-94	-0.035788567219345424
1106	95-99	0.046929788661305594
1106	100-104	0.07951149364242127
1106	105-109	0.050124543393884835
1106	110-114	-0.09948340493863128
1106	115-119	-0.19638249378627393
1106	120-124	0.05042455217977704
1106	125-129	-0.04926438757060936
1106	130-134	-0.032669693703901714
1106	135-139	-0.2503616822703094
1106	140-144	-0.11935330564675084
1106	145-149	0.026384200254760515
1106	150-151	-0.1337063273855108
1107	1	-0.11223797830418647
1107	2	0.10861957150080315
1107	3	-0.07315586601926327
1107	4	-0.06326678723996082
1107	5	-0.026235639719985215
1107	6	0.018219869349181295
1107	7	-0.5012115031593112
1107	8	-0.10433691480287166
1107	9	-0.26578958814122444
1107	10-14	-0.04190479298085137
1107	15-19	0.0064912815137248
1107	20-24	-0.030641112762985756
1107	25-29	-0.05532750713646806
1107	30-34	-0.05798068030860293
1107	35-39	-0.4010944574084476
1107	40-44	-0.7894509363355766
1107	45-49	-0.4463298592275393
1107	50-54	-0.257560290259228
1107	55-59	-0.16799804910584726
1107	60-64	-0.10565801581921619
1107	65-69	-0.597459561870977
1107	70-74	-0.37625283850924873
1107	75-79	-0.8700712596915672
1107	80-84	-0.7415381289563925
1107	85-89	-0.5691135111298138
1107	90-94	-0.8096474724835758
1107	95-99	-0.6189832786932961
1107	100-104	-0.6652453452633509
1107	105-109	-0.8090782866743105
1107	110-114	-0.7715569151937984
1107	115-119	-0.8129424156273508
1107	120-124	-0.781530251018097
1107	125-129	-0.7576808392576702
1107	130-134	-0.6419584091900923
1107	135-139	-0.8503166642631044
1107	140-144	-0.697400162687444
1107	145-149	-0.5965532726067231
1107	150-151	-0.6283720128086188
1108	1	0.16766418874819777
1108	2	0.1203095567732646
1108	3	-0.07919152770661952
1108	4	-0.12100663575668591
1108	5	0.04620545011800914
1108	6	0.009435943407666514
1108	7	-0.08805139375472493
1108	8	-0.03545936378246495
1108	9	0.004066292355304313
1108	10-14	0.017186833360405274
1108	15-19	-0.05354448537035239
1108	20-24	0.06799132990479961
1108	25-29	-0.040302785907762484
1108	30-34	-0.010839401116513159
1108	35-39	0.07970188174576975
1108	40-44	-0.00740538615674069
1108	45-49	0.0720427475471368
1108	50-54	0.03995180736333026
1108	55-59	0.016603781317037658
1108	60-64	-0.3966242476727899
1108	65-69	-0.022304396712129915
1108	70-74	-0.08529712632746467
1108	75-79	-0.22040788918873133
1108	80-84	-0.26057610497154116
1108	85-89	-0.0767507928466209
1108	90-94	-0.1942098541372701
1108	95-99	-0.17914791155675402
1108	100-104	-0.1702527090336048
1108	105-109	-0.2823793596808315
1108	110-114	-0.4202419562205151
1108	115-119	-0.14456191909884097
1108	120-124	-0.24684426322091468
1108	125-129	-0.5489521415930341
1108	130-134	-0.4756502880034752
1108	135-139	-0.33194142757048795
1108	140-144	-0.5221660999901943
1108	145-149	-0.46818697763512773
1108	150-151	-0.4489392861096917
1109	1	-0.1415552889848044
1109	2	0.04702602545294354
1109	3	0.23248098262882877
1109	4	0.02798075238502662
1109	5	0.13669945817704132
1109	6	0.15071753846758895
1109	7	0.08152184398665696
1109	8	0.1504543418012929
1109	9	0.2066671610559112
1109	10-14	0.22455770572420874
1109	15-19	0.11853934947186673
1109	20-24	0.11965154237012854
1109	25-29	0.11633544738544543
1109	30-34	0.1584015324749899
1109	35-39	0.0938941471704382
1109	40-44	-0.009163719926171154
1109	45-49	0.3358673851524472
1109	50-54	0.28528908299261246
1109	55-59	0.416360440482876
1109	60-64	0.5242847480883839
1109	65-69	0.1631239354873557
1109	70-74	-0.08842392521804143
1109	75-79	-0.2570882492284987
1109	80-84	-0.3102097854362462
1109	85-89	-0.11053068713727043
1109	90-94	-0.2688984825322649
1109	95-99	-0.20065348484682488
1109	100-104	-0.5136681153866292
1109	105-109	-0.5387219207776965
1109	110-114	-0.3407323010841914
1109	115-119	-0.43151047736981596
1109	120-124	-0.27602030592330706
1109	125-129	-0.24687720308238426
1109	130-134	-0.14516596124644465
1109	135-139	-0.29362832903053615
1109	140-144	-0.2259418077718749
1109	145-149	-0.2529062198770049
1109	150-151	-0.09330843153854573
1110	1	0.1776364329987956
1110	2	0.010588435969673071
1110	3	0.10268793303882262
1110	4	0.22275492913889394
1110	5	0.06044018445583532
1110	6	-0.025893160478133836
1110	7	-0.07197288463497387
1110	8	0.03441243108932923
1110	9	0.1466515421570449
1110	10-14	-7.459936495735064E-5
1110	15-19	0.10124272055087857
1110	20-24	0.1275247689743253
1110	25-29	0.08124889265421587
1110	30-34	0.06623831498182398
1110	35-39	-0.0016726887009639313
1110	40-44	0.3696947988815893
1110	45-49	0.2734503605526939
1110	50-54	0.2435098300966061
1110	55-59	-0.022196112946772928
1110	60-64	0.3199895508997841
1110	65-69	0.321335571567019
1110	70-74	0.6727097465247631
1110	75-79	-0.016520762569648184
1110	80-84	-8.80096512645423E-4
1110	85-89	-0.005220261522278946
1110	90-94	-4.517002413351179E-5
1110	95-99	-0.14698824991255677
1110	100-104	-0.11632593134393687
1110	105-109	0.03625400008654722
1110	110-114	-0.1003899868840108
1110	115-119	-0.04597878402086053
1110	120-124	0.06777508650152697
1110	125-129	0.027558564196024093
1110	130-134	-0.24333334377931237
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2108	100-104	0.38412093494887145
2108	105-109	0.2263665102265655
2108	110-114	0.48782466412293957
2108	115-119	0.20411503321188107
2108	120-124	0.23769784599787513
2108	125-129	0.42881980639261386
2108	130-134	0.5928663529018117
2108	135-139	0.4780143999369919
2108	140-144	0.5252877776881562
2108	145-149	0.577638260953055
2108	150-151	0.19954551940371346
2109	1	-0.15730125501151804
2109	2	-0.2711190557936902
2109	3	-0.1671659560888088
2109	4	-0.16431712357561423
2109	5	-0.4601442590336333
2109	6	-0.19677842046954197
2109	7	-0.05609407071146677
2109	8	-0.20235880512324655
2109	9	0.04143022715919642
2109	10-14	-0.23817802294323087
2109	15-19	-0.08568178883174227
2109	20-24	-0.2623735263988394
2109	25-29	-0.25560256129220704
2109	30-34	-0.07677414741611699
2109	35-39	-0.005378461512890453
2109	40-44	0.04780108737893585
2109	45-49	-0.296489476146931
2109	50-54	-0.25785387137882765
2109	55-59	-0.14239235990279298
2109	60-64	-0.2181067103808374
2109	65-69	-0.2516167275305321
2109	70-74	0.02324978376871556
2109	75-79	0.46970470656085084
2109	80-84	0.478190237250427
2109	85-89	0.36639555283096925
2109	90-94	0.3329873468072684
2109	95-99	0.41181851129644187
2109	100-104	0.511390728160432
2109	105-109	0.5410699290365528
2109	110-114	0.5204451370237386
2109	115-119	0.5196522144034326
2109	120-124	0.507839547180513
2109	125-129	0.5778980571053474
2109	130-134	0.4638633329145705
2109	135-139	0.7591848178940062
2109	140-144	0.6575493869648241
2109	145-149	0.5776144391707234
2109	150-151	0.3182596426468578
2110	1	-0.1588155981161563
2110	2	-0.03092697745585582
2110	3	-0.19275286212107545
2110	4	0.02064458629821786
2110	5	-0.08813371355626032
2110	6	-0.21822246039170778
2110	7	-0.3333600929322955
2110	8	-0.17169531883864408
2110	9	0.07504393627977635
2110	10-14	-0.05806221100425546
2110	15-19	-0.032398881292976967
2110	20-24	-0.03787569198794216
2110	25-29	-0.0020513090167710857
2110	30-34	-0.06158363834574487
2110	35-39	0.15772806036270737
2110	40-44	0.1847149659804046
2110	45-49	0.043437395989158745
2110	50-54	-0.08477308548967954
2110	55-59	0.0020288942557655787
2110	60-64	0.2649765863362532
2110	65-69	0.07906245142873303
2110	70-74	0.1732340910940522
2110	75-79	0.33814359928572557
2110	80-84	0.24222419619838575
2110	85-89	0.2031634895581007
2110	90-94	0.07857770522796059
2110	95-99	0.2914243113089974
2110	100-104	0.13086219887789596
2110	105-109	0.2188224241718295
2110	110-114	0.15004936776882616
2110	115-119	0.3903853761635254
2110	120-124	0.19465926973402148
2110	125-129	0.24395009401451873
2110	130-134	0.14441226267415885
2110	135-139	0.18751009565434273
2110	140-144	0.30264856466866874
2110	145-149	0.03478128368770328
2110	150-151	0.41749619402467886
2111	1	-0.13562353649346903
2111	2	8.949660779009605E-4
2111	3	-0.2748779854619414
2111	4	-0.020929243157176813
2111	5	-0.011590759104848303
2111	6	0.025359835817354792
2111	7	-0.21265712956851246
2111	8	-0.09601177171447972
2111	9	-0.09124342406653341
2111	10-14	0.032652211123895825
2111	15-19	0.08860089589772002
2111	20-24	-0.10429116768581537
2111	25-29	-0.02214472977622961
2111	30-34	-0.02671875627552822
2111	35-39	-0.3350815649993635
2111	40-44	0.15475109291805467
2111	45-49	0.276697255300995
2111	50-54	0.27696737349749156
2111	55-59	-0.009007328171584561
2111	60-64	-0.02208933338874175
2111	65-69	0.16284352536959545
2111	70-74	-0.015599979631982563
2111	75-79	0.06973477603716915
2111	80-84	-1.660702178796214E-4
2111	85-89	0.07212774457927296
2111	90-94	-0.10334291099892923
2111	95-99	-0.027843919266807404
2111	100-104	-0.10835542215530225
2111	105-109	-0.03499318521163275
2111	110-114	0.03007053465299947
2111	115-119	-0.19492989900597024
2111	120-124	0.018518167782854533
2111	125-129	-0.23418037674971615
2111	130-134	0.01340596035375441
2111	135-139	-0.1491452183476305
2111	140-144	-0.10593993837108684
2111	145-149	0.04936654007756047
2111	150-151	-0.25802780572068684
2112	1	-0.05579797491694194
2112	2	-0.1494445462624796
2112	3	0.04532544766804136
2112	4	-0.038400512929406716
2112	5	-0.3560988090541102
2112	6	-0.03879878873646447
2112	7	0.049678354858585294
2112	8	-0.05292132782119552
2112	9	-0.24644346795041372
2112	10-14	-0.19976073618801848
2112	15-19	-0.0741887083245345
2112	20-24	-0.007497041856616704
2112	25-29	-0.004253820400357711
2112	30-34	-0.1919572377365526
2112	35-39	-0.10922455592088198
2112	40-44	0.01018740447793931
2112	45-49	0.03691455887165773
2112	50-54	0.00838588074028479
2112	55-59	-0.03907131243607864
2112	60-64	-0.03051582201222658
2112	65-69	0.17324322131698722
2112	70-74	0.16200435233389143
2112	75-79	-0.1189188900300131
2112	80-84	0.06652175474812339
2112	85-89	-0.028919591238206976
2112	90-94	-0.03396367199242789
2112	95-99	-0.019493835613161536
2112	100-104	-0.20543009136095947
2112	105-109	-0.18787835441925083
2112	110-114	0.028277956514219937
2112	115-119	-0.21815348345697316
2112	120-124	-0.06197701478584605
2112	125-129	0.04573922171278255
2112	130-134	-0.18269816982463638
2112	135-139	-0.24613094433198057
2112	140-144	-0.15966164404347438
2112	145-149	-0.0014725432195348276
2112	150-151	0.3284273563166238
2113	1	-0.08395071403138843
2113	2	-0.09528087053254808
2113	3	-0.2304885248211761
2113	4	-0.16134114356476914
2113	5	-0.07359097425886318
2113	6	-0.021792760175202375
2113	7	0.11160387615697687
2113	8	-0.03985458178527779
2113	9	-0.018110980833327517
2113	10-14	-0.039720818125097423
2113	15-19	-0.12461035745410243
2113	20-24	-0.07699649035706813
2113	25-29	0.1473659542143153
2113	30-34	0.08098893707421695
2113	35-39	-0.05978801922135801
2113	40-44	0.04196895229498665
2113	45-49	0.13773684762398375
2113	50-54	0.14325991123909887
2113	55-59	0.2212995053589779
2113	60-64	0.4152183727108891
2113	65-69	0.3336216258783793
2113	70-74	0.9387013077062001
2113	75-79	0.7411682994232152
2113	80-84	0.8035632660319791
2113	85-89	0.6492696227144421
2113	90-94	0.6425167687403395
2113	95-99	0.6053475717903574
2113	100-104	0.8797868026168985
2113	105-109	0.5121665825814077
2113	110-114	0.7688245538495195
2113	115-119	0.6966599696305522
2113	120-124	0.6730569214036173
2113	125-129	0.4239406912110617
2113	130-134	0.8506264095475267
2113	135-139	0.7992122401256552
2113	140-144	0.816323322487218
2113	145-149	0.7714660448248871
2113	150-151	0.6717343694067139
2114	1	-0.14235811146383526
2114	2	0.0197264610651402
2114	3	-0.13432788801954842
2114	4	-0.0626787860820528
2114	5	-0.27766674407661895
2114	6	-0.14073222212570613
2114	7	-0.09564991682264434
2114	8	0.014363331252994271
2114	9	-0.3953560955952753
2114	10-14	-0.1742535409906978
2114	15-19	0.004286910403578759
2114	20-24	-0.1761795409002005
2114	25-29	0.05702630672950448
2114	30-34	-0.04770603853700095
2114	35-39	-0.08110531281444366
2114	40-44	-0.1954934171576923
2114	45-49	0.04191735455159318
2114	50-54	-0.21453163198740555
2114	55-59	0.01495064100848964
2114	60-64	0.0541303037639409
2114	65-69	0.08096459502583286
2114	70-74	0.2704402429786654
2114	75-79	-0.1570990495309097
2114	80-84	-0.10871970371395534
2114	85-89	-0.16496385126586688
2114	90-94	-0.1283375868271861
2114	95-99	0.00574497431002996
2114	100-104	0.15497248655021778
2114	105-109	-0.19710115539626827
2114	110-114	-0.056980276027495336
2114	115-119	-0.16233994059478007
2114	120-124	0.056198436201473356
2114	125-129	-0.21276876804620315
2114	130-134	-0.3137043203205039
2114	135-139	-0.1324309355035176
2114	140-144	-0.10847303864633417
2114	145-149	-0.1154597713764467
2114	150-151	-0.06570465863290664
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
16	2.0
17	11.0
18	42.0
19	86.0
20	259.0
21	982.0
22	2819.0
23	6340.0
24	10155.0
25	16321.0
26	36658.0
27	46983.0
28	19596.0
29	7708.0
30	4271.0
31	2858.0
32	2352.0
33	2162.0
34	2779.0
35	3721.0
36	6117.0
37	14653.0
38	22173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	79.90652864413914	6.00866786575332	4.308101488653324	9.776702001454211
2	7.960851096398914	76.58576020818185	7.62265125712755	7.830737438291684
3	7.4748383146454405	5.969920783743446	77.54439171864836	9.010849182962763
4	8.42246756725728	6.999827790746623	6.663541387623894	77.9141632543722
5	78.83117752860586	7.101718265661475	6.0809000803643185	7.986204125368336
6	78.20404883089051	8.119666296735678	6.302380314568904	7.373904557804906
7	7.359553786690139	76.82493972676131	8.460736290229995	7.354770196318548
8	7.757070146569209	76.2346446749072	7.782901534575791	8.225383643947803
9	77.75965328536986	6.186617427576442	7.976158585587998	8.077570701465692
10-14	21.97074356128736	35.16608625770158	6.9179327235850145	35.945237457426046
15-19	22.087101610253733	20.80873369631252	20.913398753777845	36.190765939655904
20-24	22.317192527259316	34.9543023174604	20.702250872766896	22.026254282513378
25-29	22.322050438176877	20.66319696911714	20.79235390915005	36.22239868355593
30-34	21.921855267689715	20.92772951666603	20.6720944472083	36.47832076843596
35-39	8.22213080249512	34.86022348934216	20.589625349202098	36.32802035896062
40-44	8.63074509203628	20.578718763154875	34.587845853583865	36.20269029122498
45-49	35.74757950327198	20.680035207225135	34.59492556733382	8.977459722169073
50-54	22.3061689181432	7.2691439286671	20.614978378171518	49.80970877501818
55-59	22.25460181393747	6.717787302437718	48.492786345719644	22.53482453790517
60-64	22.458382763767172	20.64224484328958	34.70035589912365	22.199016493819602
65-69	8.519861467222839	75.74930159580575	6.668899008840075	9.06193792813134
70-74	11.782557116069036	65.01482913015192	9.3363246718457	13.866289081933337
75-79	18.38884386985918	44.32497127445737	15.53102310767797	21.755161748005477
80-84	21.62110447572706	34.35217625076777	18.664170265528043	25.36254900797713
85-89	23.516434103335612	30.431051390357194	19.590905394480668	26.46160911182653
90-94	23.726339052940247	28.830071706362688	20.650284385807975	26.793304854889094
95-99	24.181979780662186	28.258758539929225	20.714058151404895	26.845203528003697
100-104	23.628415865170734	29.117239888139775	20.406359156283816	26.84798509040568
105-109	23.71873555949512	29.058098785251573	20.699324650772983	26.523841004480325
110-114	23.85186106473664	28.69153826651423	20.555473171105064	26.901127497644072
115-119	24.095515271192344	28.282607946913423	20.63600947537207	26.985867306522167
120-124	24.105755615935095	28.122153763728903	20.65506486548544	27.11702575485056
125-129	24.552517754346624	28.005323194643893	20.578395139092855	26.863763911916628
130-134	24.071719482338487	28.13203487432061	20.404711266992525	27.391534376348375
135-139	24.19580593529327	27.864727588618486	20.79725306843698	27.142213407651266
140-144	24.580477518952655	27.7776502148797	20.71187750900275	26.929994757164888
145-149	24.27872748355395	27.913217684809936	20.924269734299237	26.883785097336872
150-151	24.466151314530634	28.249971298457773	20.468265661474877	26.81561172553672
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.5
8	1.0
9	1.0
10	1.0
11	1.5
12	3.5
13	3.0
14	0.5
15	1.0
16	2.5
17	6.5
18	8.5
19	9.0
20	15.5
21	25.5
22	41.5
23	67.0
24	80.5
25	93.0
26	109.0
27	128.5
28	155.5
29	190.0
30	227.5
31	251.0
32	268.5
33	307.5
34	418.5
35	669.0
36	1196.5
37	2176.5
38	3660.5
39	5438.0
40	7545.5
41	9898.0
42	12428.5
43	13968.5
44	14634.5
45	15065.0
46	14657.5
47	13690.0
48	12397.0
49	10736.0
50	8859.0
51	7354.0
52	6228.0
53	5621.0
54	4743.0
55	3707.0
56	3192.0
57	2585.5
58	2144.5
59	2043.5
60	1820.0
61	1625.5
62	1441.5
63	1116.5
64	937.0
65	938.0
66	1170.0
67	1202.0
68	938.0
69	806.0
70	720.0
71	628.5
72	584.0
73	586.0
74	693.0
75	752.0
76	631.0
77	509.0
78	509.5
79	527.0
80	400.0
81	272.5
82	285.5
83	332.0
84	332.5
85	296.0
86	252.5
87	239.5
88	231.5
89	206.5
90	210.5
91	212.0
92	182.0
93	145.5
94	111.5
95	59.0
96	30.0
97	17.0
98	19.0
99	16.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	9.5671807431786E-5
20-24	9.5671807431786E-5
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	2.8701542229535803E-4
80-84	1.91343614863572E-4
85-89	4.7835903715893005E-4
90-94	4.7835903715893005E-4
95-99	2.8701542229535803E-4
100-104	6.697026520225021E-4
105-109	2.8701542229535803E-4
110-114	4.7835903715893005E-4
115-119	3.82687229727144E-4
120-124	0.0
125-129	2.8701542229535803E-4
130-134	9.5671807431786E-5
135-139	9.5671807431786E-5
140-144	3.82687229727144E-4
145-149	3.82687229727144E-4
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	209048.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.8631127779266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.37058890275729	11.52940951360453
2	16.075683649154655	5.421721327159313
3	6.025190060138432	3.048103784776702
4	3.14024736185181	2.118173816539742
5	1.6509701577215479	1.3920247981324863
6	1.0552592760694428	1.0676973709387316
7	0.760240553727448	0.8974015537101526
8	0.507772608646318	0.6850101412115878
9	0.3744468398956088	0.5682905361448088
>10	1.8325201407012368	5.2093299146607475
>50	0.12481561329853626	1.496785427270292
>100	0.06524452513332576	2.0674677586008956
>500	0.011346873936230567	1.2466036508361715
>1k	0.002836718484057642	0.7438483027821362
>5k	0.0	0.0
>10k+	0.002836718484057642	62.508132103631695
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC	130672	62.508132103631695	TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATATCGTATGC	1555	0.7438483027821362	TruSeq Adapter, Index 6 (98% over 50bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA	868	0.41521564425395124	No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT	709	0.3391565573456814	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGC	526	0.25161685354559715	TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGGATGC	503	0.24061459569094176	TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGCATGC	461	0.22052351613026672	TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGC	327	0.1564234051509701	TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATCTCGTATGCCG	310	0.1482913015192683	TruSeq Adapter, Index 6 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAGCTCGTATGC	287	0.13728904366461292	TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGAATGC	280	0.13394053040450038	TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATGTCGTATGC	220	0.1052389881749646	TruSeq Adapter, Index 6 (98% over 50bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.00334851326011251	0.0	0.0	0.0	0.0
2	0.00334851326011251	0.0	0.0	0.0	0.0
3	0.00382687229727144	0.0	0.0	0.0	0.0
4	0.00382687229727144	0.0	0.0	0.0	0.0
5	0.00382687229727144	0.0	0.0	0.0	0.0
6	0.00382687229727144	0.0	0.0	0.0	0.0
7	0.00430523133443037	0.0	0.0	0.0	0.0
8	0.00430523133443037	0.0	0.0	0.0	0.0
9	0.00430523133443037	0.0	0.0	0.0	0.0
10-14	0.005070605793884658	0.0	0.0	0.0	0.0
15-19	0.005931652060770731	0.0	0.0	0.0	0.0
20-24	0.007462400979679308	0.0	0.0	0.0	0.0
25-29	0.008036431824270026	0.0	0.0	0.0	0.0
30-34	0.010141211587769317	0.0	0.0	0.0	0.0
35-39	0.012341663158700394	0.0	0.0	0.0	0.0
40-44	0.015785848226244688	0.0	0.0	0.0	0.0
45-49	0.019899735945811486	0.0	0.0	0.0	0.0
50-54	0.027170793310627224	0.0	0.0	0.0	0.0
55-59	0.0382687229727144	0.0	0.0	0.0	0.0
60-64	0.05845547434082125	0.0	0.0	0.0	0.0
65-69	0.08438253415483525	0.0	0.0	0.0	0.0
70-74	0.10370823925605603	0.0	0.0	0.0	0.0
75-79	0.13260112510045538	0.0	0.0	0.0	0.0
80-84	0.18091538785350733	0.0	0.0	0.0	0.0
85-89	0.24329340629903182	0.0	0.0	0.0	0.0
90-94	0.3273889250315717	0.0	0.0	0.0	0.0
95-99	0.4338716467031495	0.0	0.0	0.0	0.0
100-104	0.5765183115839425	0.0	0.0	0.0	0.0
105-109	0.7756113428494891	0.0	0.0	0.0	0.0
110-114	1.0265584937430638	0.0	0.0	0.0	0.0
115-119	1.3199839271363516	0.0	0.0	0.0	0.0
120-124	1.6861199341777966	0.0	0.0	0.0	0.0
125-129	2.140848034901075	0.0	0.0	0.0	0.0
130-134	2.7223412804714706	0.0	0.0	0.0	0.0
135-139	3.382859438980521	3.82687229727144E-4	0.0	0.0	0.0
140	3.805824499636447	4.7835903715893E-4	0.0	0.0	0.0
>>END_MODULE