FastQCFastQC Report
Fri 12 Jan 2024
DRR051417_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR051417_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640081
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT11210.17513408459241878No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT9620.15029347848162966No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA8620.13467045577044157No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC8070.1260777932792881No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG7810.12201580737437917No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC7620.11904743305925344No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCACTCCGC7420.11592282851701581No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCAACCGATCCCGG7110.1110796914765475No Hit
CGACGGGCGCTGACCCCCCTCGCGGGGGGGATGCGTGCATTTATCAGATC6610.10326818012095344No Hit
CGGGCGCTGACCCCCCTCGCGGGGGGGATGCGTGCATTTATCAGATCAAA6590.10295571966672967No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG6560.10248702898539404No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)