##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename DRR051417_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 640081 Sequences flagged as poor quality 0 Sequence length 151 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.72252105592886 33.0 32.0 34.0 29.0 34.0 2 31.848680088926244 33.0 32.0 34.0 29.0 34.0 3 31.92818408920121 33.0 32.0 34.0 30.0 34.0 4 31.990544946655188 33.0 32.0 34.0 30.0 34.0 5 31.97902452970796 33.0 32.0 34.0 30.0 34.0 6 34.02272993574251 35.0 33.0 37.0 32.0 37.0 7 33.91713548753986 35.0 33.0 37.0 31.0 37.0 8 34.15761130231955 37.0 33.0 37.0 32.0 37.0 9 34.25460840112423 37.0 33.0 37.0 32.0 37.0 10-14 34.912851654712455 37.4 34.6 37.4 32.0 37.4 15-19 36.0242309957646 38.0 37.0 38.0 33.0 38.0 20-24 36.11378872361466 38.0 37.0 38.4 32.2 38.4 25-29 36.21189474457139 38.0 37.0 39.0 32.0 39.0 30-34 36.11122029868095 38.0 37.0 39.0 32.0 39.0 35-39 35.99930633779162 38.0 37.0 39.0 32.0 39.0 40-44 35.82697439855268 38.0 37.0 39.0 30.8 39.0 45-49 35.69477144298925 38.0 37.0 39.0 30.0 39.0 50-54 35.544769802571864 38.0 36.8 39.0 29.2 39.0 55-59 35.42245715776597 38.0 36.0 39.0 27.0 39.0 60-64 35.27540264435282 38.0 36.0 39.0 26.2 39.0 65-69 35.12979013593592 38.0 36.0 39.0 24.8 39.0 70-74 34.92607935558156 38.0 36.0 39.0 23.0 39.0 75-79 34.737132644149725 38.0 36.0 39.0 18.0 39.0 80-84 34.56174421674756 38.0 35.2 39.0 17.0 39.0 85-89 34.430740484407444 38.0 35.0 39.0 16.4 39.0 90-94 34.225837667420215 38.0 34.4 39.0 16.0 39.0 95-99 34.034099746750805 38.0 34.0 39.0 15.8 39.0 100-104 33.93241042930504 38.0 34.0 39.0 15.4 39.0 105-109 33.69777012596843 38.0 33.2 39.0 14.8 39.0 110-114 33.49150310663806 38.0 33.0 39.0 14.0 39.0 115-119 33.27343726809576 38.0 32.8 39.0 14.0 39.0 120-124 32.95914798283342 37.8 31.8 39.0 14.0 39.0 125-129 32.77050092097719 37.0 31.4 39.0 14.0 39.0 130-134 32.46902032711485 37.0 30.6 39.0 13.0 39.0 135-139 32.23292739512655 37.0 29.8 38.4 13.0 39.0 140-144 31.954802282836077 37.0 27.8 38.0 13.0 39.0 145-149 31.652267134940736 37.0 26.4 38.0 13.0 39.0 150-151 29.510460394856274 34.5 20.0 37.5 12.5 38.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07914166907802667 1101 2 0.18696775183097714 1101 3 0.07681671293555326 1101 4 -0.0425092805322862 1101 5 0.20190431553177035 1101 6 -0.06461655084927287 1101 7 0.3648601824448292 1101 8 0.12396517450498834 1101 9 0.07845018405139115 1101 10-14 -0.031175117360469073 1101 15-19 -0.0020626691578229384 1101 20-24 -0.08470540636725588 1101 25-29 -0.015280959040943287 1101 30-34 0.03504329554047558 1101 35-39 -0.01741594359227605 1101 40-44 -0.00451929662885675 1101 45-49 -0.10573500487526388 1101 50-54 -0.13266402540821076 1101 55-59 -0.13066909034476026 1101 60-64 -0.0016416859959704766 1101 65-69 -0.08832645856409727 1101 70-74 -0.1189099205168418 1101 75-79 -0.14157388737520904 1101 80-84 -0.14470317729016813 1101 85-89 -0.26551578340672677 1101 90-94 -0.13043156225145935 1101 95-99 -0.23989651837596426 1101 100-104 -0.23606337968026736 1101 105-109 -0.23243090227200014 1101 110-114 -0.277757234283591 1101 115-119 -0.16852652938241164 1101 120-124 -0.15513610358767238 1101 125-129 -0.07505572496536672 1101 130-134 -0.09987817527992604 1101 135-139 -0.2048905456376815 1101 140-144 -0.2685705542840857 1101 145-149 -0.34144155357200034 1101 150-151 -0.21067008563570155 1102 1 0.02528116136693015 1102 2 0.13664824379524632 1102 3 -0.09503171036456592 1102 4 0.03835458864839225 1102 5 -0.047606973949577025 1102 6 0.1056805103727072 1102 7 0.42453598441684903 1102 8 0.10292128661615862 1102 9 0.09059400653203653 1102 10-14 0.14471047496111566 1102 15-19 0.11958403265231254 1102 20-24 0.031091094609799086 1102 25-29 0.050347209341246923 1102 30-34 0.044539363831511025 1102 35-39 0.030984036122674752 1102 40-44 0.054809660612257005 1102 45-49 0.06715287913866774 1102 50-54 0.0782616206317428 1102 55-59 -0.0459941860231865 1102 60-64 0.02984789181958547 1102 65-69 0.057083623327535804 1102 70-74 0.010991056608069982 1102 75-79 -0.03645091296508696 1102 80-84 -0.02583030791441132 1102 85-89 -0.34577735689727973 1102 90-94 0.12394584405326015 1102 95-99 0.018005970503345736 1102 100-104 0.004824838962953493 1102 105-109 -0.10650572078928633 1102 110-114 -0.015144542135494987 1102 115-119 0.11798650394688082 1102 120-124 0.1693707161496789 1102 125-129 -0.027760984308599745 1102 130-134 0.008380319917371537 1102 135-139 0.13049946219871345 1102 140-144 0.29378235597269864 1102 145-149 0.20473965632754698 1102 150-151 0.16152993901729573 1103 1 -0.010352618075557984 1103 2 -0.050653856401815744 1103 3 0.12244696142665745 1103 4 0.07217106951867436 1103 5 0.07634777255000103 1103 6 -0.07265654328458737 1103 7 0.26304315084886554 1103 8 -0.11857738663314876 1103 9 -0.0023051021655362547 1103 10-14 0.04608880827797179 1103 15-19 -0.06778899366178592 1103 20-24 -0.07630815531720714 1103 25-29 -0.12852937371978612 1103 30-34 -0.008195596333003152 1103 35-39 -0.1034505570651234 1103 40-44 -0.01860568470744539 1103 45-49 -0.15249259285522498 1103 50-54 -0.11319097628626906 1103 55-59 -0.09276497779824666 1103 60-64 -0.06759785208218005 1103 65-69 0.07195557714056378 1103 70-74 -0.020827256764469837 1103 75-79 0.10825331303717434 1103 80-84 -0.03756575935950934 1103 85-89 0.11246126479139207 1103 90-94 0.10388657531765233 1103 95-99 -0.14018476872924168 1103 100-104 0.14531365033136012 1103 105-109 -0.50962643330368 1103 110-114 -0.2263834008551271 1103 115-119 0.12745832785081745 1103 120-124 -0.019080709534883056 1103 125-129 0.007973542459225769 1103 130-134 -0.08355172436309743 1103 135-139 0.18439917135034278 1103 140-144 0.07974019376657893 1103 145-149 0.13671194824859967 1103 150-151 0.25956487159535513 1104 1 -0.05546135988480927 1104 2 -0.03416292747433758 1104 3 -0.05733525008144014 1104 4 -0.04356268453995327 1104 5 -0.07039759500754883 1104 6 0.0022149199117507123 1104 7 0.37541567565794054 1104 8 0.03296647049446477 1104 9 0.17819791716997457 1104 10-14 -0.0936253075782858 1104 15-19 0.056357741906275294 1104 20-24 0.13460010609866657 1104 25-29 0.08094053854200212 1104 30-34 0.1490446025315677 1104 35-39 0.10540623300994412 1104 40-44 0.06101200404729923 1104 45-49 0.07594610690327386 1104 50-54 0.008359762269549265 1104 55-59 0.049964118230334975 1104 60-64 0.1638969857578374 1104 65-69 0.15812717295683143 1104 70-74 0.36570212976491234 1104 75-79 0.15525335876818502 1104 80-84 0.2543349520843421 1104 85-89 0.20290773011095098 1104 90-94 0.19083091632290206 1104 95-99 0.17430259753477628 1104 100-104 0.22853091400546788 1104 105-109 0.307855007528012 1104 110-114 0.16844071985730835 1104 115-119 0.042623710782692115 1104 120-124 -0.14528218294447726 1104 125-129 -0.2632069926807006 1104 130-134 0.14593835309652547 1104 135-139 0.24011010345721928 1104 140-144 0.32781981798833826 1104 145-149 0.33356587251369874 1104 150-151 0.20677576994838276 1105 1 -0.0027072827706504654 1105 2 0.09172791746932063 1105 3 0.11794537777614167 1105 4 0.11798283873469728 1105 5 0.0727215323280177 1105 6 -0.03216748279552917 1105 7 0.14385971209095771 1105 8 -0.03450589873187937 1105 9 -0.1606871316113896 1105 10-14 -0.09144825462079353 1105 15-19 0.07335316977824391 1105 20-24 0.05015419761322448 1105 25-29 -0.025363021957694798 1105 30-34 -0.06510854575658698 1105 35-39 -0.0012582735962425318 1105 40-44 -0.002503710392701919 1105 45-49 -0.05868275870496831 1105 50-54 -0.12475547695715505 1105 55-59 -0.1560761308966363 1105 60-64 -0.029116586622187413 1105 65-69 -0.16272806698989228 1105 70-74 -0.05628368583791854 1105 75-79 -0.2510290331387921 1105 80-84 0.06238024135244302 1105 85-89 -0.179565899150667 1105 90-94 -0.008353372837191841 1105 95-99 -0.05569070627772277 1105 100-104 0.012854419063614841 1105 105-109 0.048364493793627616 1105 110-114 0.05635186936950021 1105 115-119 -0.07057173868989253 1105 120-124 0.07132205797426394 1105 125-129 0.06645265378939769 1105 130-134 -0.0448595732454109 1105 135-139 0.13571718845559388 1105 140-144 0.10893354517270026 1105 145-149 -0.007903256621926147 1105 150-151 -0.0040466730800226 1106 1 0.14157133324959048 1106 2 0.08266252242354355 1106 3 -0.014085636183782668 1106 4 0.12258761095318249 1106 5 0.005692429439569935 1106 6 -0.1798321534542069 1106 7 0.36349861058576494 1106 8 0.17733400808063493 1106 9 0.04365445876408813 1106 10-14 0.1167320777236327 1106 15-19 0.07549560830545232 1106 20-24 -0.022373039705442466 1106 25-29 -0.011774466545723783 1106 30-34 0.1653846562891701 1106 35-39 0.04658913189672376 1106 40-44 -0.07451317474667007 1106 45-49 -0.013661118095605218 1106 50-54 -0.09930677557571954 1106 55-59 -0.020311429296796746 1106 60-64 -0.21293086687627039 1106 65-69 -0.08573984283030711 1106 70-74 -0.05023741970757101 1106 75-79 -0.04341015539277038 1106 80-84 -0.07131679011652636 1106 85-89 -0.04791527941748086 1106 90-94 -0.46234518253087487 1106 95-99 -0.16758273983978 1106 100-104 -0.1645227900440034 1106 105-109 0.013608692326663174 1106 110-114 -0.03880077973957441 1106 115-119 -0.036181188483311644 1106 120-124 0.014283963949552003 1106 125-129 -0.01972036188138304 1106 130-134 -0.04488876713725887 1106 135-139 -0.25193459612114566 1106 140-144 -0.017895423030715563 1106 145-149 -0.07680207903788272 1106 150-151 0.18678764612351628 1107 1 0.03854964553258711 1107 2 0.23878137715106718 1107 3 0.06635767886679034 1107 4 0.026421959063679168 1107 5 -0.022536826334597748 1107 6 0.022706466784306656 1107 7 0.2798725853253572 1107 8 0.016248615839906222 1107 9 0.030961357571513304 1107 10-14 0.05531055102912319 1107 15-19 0.08304957600031315 1107 20-24 0.08726415649039865 1107 25-29 0.083407941746934 1107 30-34 0.1686171828327261 1107 35-39 0.030309757871663123 1107 40-44 -0.03826197965785383 1107 45-49 0.07705190963045538 1107 50-54 0.08329172490230974 1107 55-59 -0.0029436438592682634 1107 60-64 0.03621722349760148 1107 65-69 -0.0990709956507132 1107 70-74 0.06584218578732504 1107 75-79 0.13198258853115163 1107 80-84 0.005304253510509227 1107 85-89 -0.03103559341135309 1107 90-94 -0.1874365124760331 1107 95-99 0.010993720684545849 1107 100-104 -0.0835028131909965 1107 105-109 0.05746014907702346 1107 110-114 0.12117003493359135 1107 115-119 -0.0032661781678058333 1107 120-124 -0.06494363128015124 1107 125-129 0.0047641145439172305 1107 130-134 -0.03550216219588265 1107 135-139 -0.3266253826153829 1107 140-144 -0.17744657283195053 1107 145-149 -0.21293955199113768 1107 150-151 -0.0677763351576175 1108 1 0.09097322365948912 1108 2 0.14356155008980664 1108 3 0.015687845716136906 1108 4 0.07503966701642639 1108 5 -0.040196063942683935 1108 6 -0.22444369762928318 1108 7 0.01307195202856093 1108 8 -0.8514593369064087 1108 9 0.03592421227861564 1108 10-14 -0.4507654445865157 1108 15-19 -0.009453164226208344 1108 20-24 0.06970965642950233 1108 25-29 0.17474639016977989 1108 30-34 -0.10709588500311185 1108 35-39 -0.12003246597438988 1108 40-44 -0.03514908232500602 1108 45-49 -0.019060622338855637 1108 50-54 0.10782466851453876 1108 55-59 0.1598684374950139 1108 60-64 0.22465468543889955 1108 65-69 0.030707350470343897 1108 70-74 0.04328098739492248 1108 75-79 -0.06677030393018413 1108 80-84 0.005519817997168275 1108 85-89 0.07331832206788391 1108 90-94 -0.12773854633304893 1108 95-99 -0.06609189617088163 1108 100-104 0.0387271222614487 1108 105-109 0.055630895636518574 1108 110-114 0.05983767590302591 1108 115-119 -0.15761278637631193 1108 120-124 0.23305052290325534 1108 125-129 0.05936647722812438 1108 130-134 0.2150909446120508 1108 135-139 0.06917415459080445 1108 140-144 0.20763685184227043 1108 145-149 0.027861812032735855 1108 150-151 0.11078291462925094 1109 1 0.21160951012330997 1109 2 0.09753912717513202 1109 3 0.27484749821413246 1109 4 0.13009704810651357 1109 5 0.34503090504306044 1109 6 0.10874893638139582 1109 7 0.1254885406595534 1109 8 0.2507093496455255 1109 9 0.1679348996400165 1109 10-14 -0.17022350985870105 1109 15-19 -0.1247752119547485 1109 20-24 0.2048487516351969 1109 25-29 0.28522281109731296 1109 30-34 0.0346067793501561 1109 35-39 0.04917763934754049 1109 40-44 0.15047701700147798 1109 45-49 0.09979467835440659 1109 50-54 0.19842747287990647 1109 55-59 0.15840820277671952 1109 60-64 0.2609733762769153 1109 65-69 0.24106951197362747 1109 70-74 0.2750218865178482 1109 75-79 0.12178631995159606 1109 80-84 0.26017000294986303 1109 85-89 0.2780004265969893 1109 90-94 0.1725273356003214 1109 95-99 0.09312222758158128 1109 100-104 0.0840167512745964 1109 105-109 0.18337416169097054 1109 110-114 0.11608416520174103 1109 115-119 0.09226110869093418 1109 120-124 0.20164610856146936 1109 125-129 0.24695426510562868 1109 130-134 0.35375016325699704 1109 135-139 0.21564609194813755 1109 140-144 0.34929144694108416 1109 145-149 -0.136194936452938 1109 150-151 0.3952711579168273 1110 1 0.09039344818774708 1110 2 0.07252843919427221 1110 3 0.11497643671475899 1110 4 0.22486399232654009 1110 5 0.14808289026213117 1110 6 0.037640946323250546 1110 7 0.2881947392188451 1110 8 0.2575787366494069 1110 9 0.0939159135957155 1110 10-14 0.08403512898291865 1110 15-19 0.11800369557917634 1110 20-24 0.12472604801584453 1110 25-29 0.04026668013374035 1110 30-34 0.016127906151432114 1110 35-39 0.02274723350048191 1110 40-44 -0.019786175543110573 1110 45-49 -0.07799379191963851 1110 50-54 -0.049656177119288714 1110 55-59 -0.013355190342480228 1110 60-64 -0.09108472154606062 1110 65-69 -0.13388606088498278 1110 70-74 -0.1770820337088992 1110 75-79 -0.17701126188256922 1110 80-84 -0.0864484680123283 1110 85-89 -0.18517824664122173 1110 90-94 -0.03518155685393509 1110 95-99 -0.1893860225281898 1110 100-104 -0.19041320953343188 1110 105-109 0.016739012601995285 1110 110-114 -0.1599340286586397 1110 115-119 -0.0595030177312168 1110 120-124 -0.01526638618032905 1110 125-129 0.01621593920737041 1110 130-134 0.11597949815664066 1110 135-139 0.14004775918284196 1110 140-144 0.2358979936025385 1110 145-149 -0.05267711240482953 1110 150-151 -0.17923928559961055 1111 1 0.17310933192335654 1111 2 0.19346408032312823 1111 3 0.22082493918206936 1111 4 0.24611154696109594 1111 5 0.2451736300918128 1111 6 0.130504371938585 1111 7 0.45410191389206744 1111 8 0.21622990537258602 1111 9 0.17062046375614415 1111 10-14 0.05199632780416863 1111 15-19 0.21737009538836105 1111 20-24 0.2535537799922025 1111 25-29 0.1924814648007782 1111 30-34 0.1784296058564081 1111 35-39 0.19847827560301567 1111 40-44 0.18146909824672264 1111 45-49 0.20202635076878295 1111 50-54 0.20386771664790615 1111 55-59 0.23584133835670684 1111 60-64 0.19490567423457605 1111 65-69 0.2916365143312376 1111 70-74 0.1924206771060213 1111 75-79 0.27482766949480464 1111 80-84 0.27485338574445706 1111 85-89 0.2249364971109813 1111 90-94 0.27944690496358504 1111 95-99 0.35439964336490704 1111 100-104 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0.0691000578910419 2112 3 -0.10586345667784869 2112 4 -0.08103047580462786 2112 5 0.03188189728734869 2112 6 0.061243439317273385 2112 7 0.3452856440134724 2112 8 0.3161609711132627 2112 9 0.18902467242296694 2112 10-14 0.18877300673656094 2112 15-19 0.09410244119294475 2112 20-24 0.013464889245447864 2112 25-29 0.015301143074033519 2112 30-34 0.04000934220318442 2112 35-39 0.12771065914532898 2112 40-44 0.17225222781564753 2112 45-49 0.09801035925919166 2112 50-54 0.01846637488763747 2112 55-59 0.13012474912012806 2112 60-64 -0.013774275340075803 2112 65-69 -0.03686182191855636 2112 70-74 -0.010543766982323177 2112 75-79 0.028316706780060485 2112 80-84 -0.3302974858423511 2112 85-89 -0.08291186764530778 2112 90-94 0.10227738203432324 2112 95-99 0.03247645881113215 2112 100-104 -0.06779755525970899 2112 105-109 0.07108713612966966 2112 110-114 0.015149255986386834 2112 115-119 -0.08424704679099904 2112 120-124 0.0887053986093278 2112 125-129 -0.17540094342196966 2112 130-134 -0.6753548588699019 2112 135-139 -0.4912249628234733 2112 140-144 -0.45848777713820255 2112 145-149 -0.20305066895358337 2112 150-151 -0.21719968551835578 2113 1 0.012710239796863476 2113 2 0.018140295208986856 2113 3 0.1942908539082886 2113 4 0.08913976540677027 2113 5 -0.0038097606633336056 2113 6 0.07950656503441422 2113 7 0.5455222641517139 2113 8 0.23822500604169505 2113 9 0.2608163642331718 2113 10-14 0.2988808259061173 2113 15-19 0.18900234151750084 2113 20-24 0.21683862932647457 2113 25-29 0.14687066253056003 2113 30-34 0.04093536431258116 2113 35-39 0.16162132235573523 2113 40-44 0.15326290664742004 2113 45-49 0.23216366912610908 2113 50-54 0.18727264056352766 2113 55-59 0.09535744805668145 2113 60-64 0.2688419155356243 2113 65-69 0.22353776796101243 2113 70-74 0.1210018049937247 2113 75-79 0.24942823463725006 2113 80-84 -0.020735225168486693 2113 85-89 0.2649543880551093 2113 90-94 0.16392953615777373 2113 95-99 0.18220681176686782 2113 100-104 0.252375818155258 2113 105-109 0.1384798660869393 2113 110-114 0.23964998670172832 2113 115-119 0.09711610406306903 2113 120-124 0.22320182353595897 2113 125-129 0.14521442753005687 2113 130-134 0.11528659263288432 2113 135-139 0.27764410551645113 2113 140-144 0.23401459976818373 2113 145-149 0.39396192250477924 2113 150-151 0.1908049184244156 2114 1 0.2785860902179884 2114 2 0.1160335683501259 2114 3 0.15560800265221886 2114 4 0.16481613393793282 2114 5 0.2536408490366284 2114 6 0.17018018745537233 2114 7 0.4714007019483546 2114 8 0.21845261571942842 2114 9 0.1069568095157365 2114 10-14 0.1309689649323218 2114 15-19 0.19330032145978748 2114 20-24 0.23732921477177626 2114 25-29 0.24648827065269785 2114 30-34 0.22193545637869505 2114 35-39 0.28982347733794 2114 40-44 0.254938827546745 2114 45-49 0.3830417894533511 2114 50-54 0.4032795264745488 2114 55-59 0.3824882708420958 2114 60-64 0.37068109506773084 2114 65-69 0.3575608829071584 2114 70-74 0.32600678926312554 2114 75-79 0.5059574688058461 2114 80-84 0.29238054639324673 2114 85-89 0.38287061423299207 2114 90-94 0.3451010616934198 2114 95-99 0.45670394025624006 2114 100-104 0.5037469684420941 2114 105-109 0.4724621068493775 2114 110-114 0.36255357759797135 2114 115-119 0.3469238763486189 2114 120-124 0.3939214631447143 2114 125-129 0.3287676084145801 2114 130-134 0.4043767571137735 2114 135-139 0.4637441845663304 2114 140-144 0.443816943327203 2114 145-149 0.48936067992959664 2114 150-151 -0.04895649539375668 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 0.0 11 0.0 12 0.0 13 10.0 14 157.0 15 1183.0 16 3481.0 17 2904.0 18 3140.0 19 4052.0 20 5038.0 21 6295.0 22 7351.0 23 8802.0 24 9881.0 25 11184.0 26 12092.0 27 13446.0 28 14381.0 29 15843.0 30 17441.0 31 19515.0 32 22568.0 33 26931.0 34 33135.0 35 43790.0 36 65319.0 37 137511.0 38 154630.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.030886715900017 15.793001198285841 10.184336044969308 43.99177604084483 2 29.581412352499136 18.978847989551323 25.240399261968406 26.199340395981135 3 26.84738337804122 22.065176126146536 19.619548150937145 31.467892344875104 4 30.294603339264874 25.993272726420564 18.620924539238004 25.091199395076565 5 30.828442025306174 26.003583921409945 19.901856171328316 23.266117881955566 6 25.802359388889844 28.60372359123298 21.30246015738633 24.29145686249084 7 24.57548341538024 19.71516104992962 29.291605281206596 26.417750253483547 8 23.86135504725183 19.46941090268263 26.0699817679325 30.599252282133044 9 24.81389074195297 18.207695588527077 27.93818282373637 29.040230845783583 10-14 27.45296298437229 22.59414042910194 22.278274155927143 27.674622430598628 15-19 27.54962421919989 21.769164695821118 22.651178617192897 28.030032467786086 20-24 28.183655735640627 22.108979468510352 22.281114147883343 27.426250647965677 25-29 28.018650145419137 22.40579626822114 22.081680937781606 27.493872648578115 30-34 28.02714907242961 22.15745262160652 21.94744788470456 27.86795042125932 35-39 27.553913539611585 21.982207235216077 22.18158843795248 28.282290787219853 40-44 28.401793154778176 21.911402755849046 21.898966783141947 27.787837306230827 45-49 28.687230951852 21.88743105165193 21.687581220240077 27.73775677625599 50-54 28.461589657046098 22.219340638876194 21.3679785751848 27.951091128892912 55-59 28.555774278215225 21.572490938632672 21.431946006749154 28.439788776402953 60-64 28.41769745153173 22.384688380458275 21.175743104050035 28.021871063959956 65-69 28.64864679076813 21.748429567377997 21.51233348914758 28.090590152706298 70-74 28.46308490381509 21.708862460231508 21.095063801439697 28.732988834513705 75-79 28.715767897400163 21.451977021586398 21.377986154834012 28.454268926179427 80-84 28.524831630105545 20.995258708771484 21.453733509061696 29.02617615206128 85-89 28.737350365846154 21.692987946152794 21.106153033271895 28.463508654729157 90-94 28.82862474346892 21.291298065986915 21.47921254646306 28.400864644081096 95-99 28.978682890537925 21.296393217445264 20.86910114308051 28.855822748936298 100-104 28.54102156299701 21.60161730289121 21.185168057642976 28.672193076468805 105-109 28.44812232493874 21.674112624883062 21.750384483910416 28.127380566267778 110-114 29.090075668973785 22.023805246053055 21.032925080623063 27.8531940043501 115-119 28.67654962267122 22.016459233743866 21.09885289052091 28.208138253064003 120-124 29.34795990233678 22.496005169376442 20.760964458662173 27.3950704696246 125-129 30.174762911929882 22.835164306383625 20.46233072000845 26.52774206167805 130-134 30.433040973131398 23.007102259412136 20.184727486337604 26.375129281118863 135-139 30.649363391280325 23.422632699203476 20.183502291591417 25.74450161792478 140-144 31.1917902428675 23.673355753538555 20.143251425530725 24.991602578063215 145-149 31.559915160392855 23.64910298488435 20.5125497126602 24.2784321420626 150-151 32.10689618000278 23.881661482410767 19.697099889232422 24.314342448354033 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 1.5 3 2.5 4 3.5 5 3.5 6 3.0 7 4.0 8 5.5 9 7.5 10 8.0 11 13.0 12 23.0 13 28.5 14 35.0 15 44.5 16 57.0 17 70.5 18 109.5 19 168.5 20 225.5 21 326.0 22 477.5 23 629.5 24 800.0 25 1062.0 26 1340.5 27 1650.5 28 2002.5 29 2390.0 30 2813.0 31 3107.0 32 3449.0 33 3923.5 34 4383.0 35 4806.5 36 5418.0 37 6171.0 38 7008.5 39 7858.0 40 8883.0 41 10167.5 42 11296.0 43 12460.5 44 14373.0 45 15778.0 46 16543.0 47 18102.5 48 18982.0 49 17975.0 50 16703.0 51 16595.0 52 16450.0 53 17560.0 54 19282.5 55 20459.0 56 20959.5 57 18581.5 58 17401.0 59 19127.5 60 19273.5 61 17437.5 62 15190.0 63 12723.5 64 11243.5 65 11263.5 66 13268.5 67 13742.0 68 11289.5 69 9710.5 70 8363.5 71 6875.5 72 6263.0 73 6614.0 74 8328.0 75 8831.0 76 7161.0 77 5953.5 78 5780.5 79 5675.0 80 4585.5 81 3675.0 82 3471.0 83 3385.0 84 3525.0 85 3309.5 86 2889.5 87 2819.5 88 2634.0 89 2448.0 90 2420.0 91 2274.5 92 2010.0 93 1721.0 94 1491.0 95 1154.0 96 743.5 97 516.0 98 520.0 99 762.0 100 553.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 6.249209084475247E-5 20-24 1.2498418168950493E-4 25-29 3.1246045422376234E-5 30-34 3.1246045422376234E-5 35-39 9.373813626712869E-5 40-44 3.7495254506851475E-4 45-49 6.249209084475247E-5 50-54 1.2498418168950493E-4 55-59 1.5623022711188115E-4 60-64 3.7495254506851475E-4 65-69 4.06198590490891E-4 70-74 2.1872231795663362E-4 75-79 3.124604542237623E-4 80-84 3.4370649964613853E-4 85-89 4.999367267580197E-4 90-94 4.06198590490891E-4 95-99 5.624288176027722E-4 100-104 4.6869068133564346E-4 105-109 3.4370649964613853E-4 110-114 5.624288176027722E-4 115-119 4.06198590490891E-4 120-124 5.936748630251484E-4 125-129 3.7495254506851475E-4 130-134 4.6869068133564346E-4 135-139 2.812144088013861E-4 140-144 5.624288176027722E-4 145-149 4.06198590490891E-4 150-151 3.124604542237623E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 640081.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.126186973776385 #Duplication Level Percentage of deduplicated Percentage of total 1 85.31738484090383 34.23461336239741 2 5.620337382783788 4.510454173545746 3 2.3182282919588735 2.7906498567312004 4 1.2878781576236933 2.067105590090039 5 0.7840985146220435 1.5731441801792225 6 0.552312483202946 1.3297316381371282 7 0.3991781934412293 1.1212249178113929 8 0.3009633049931298 0.966120787872001 9 0.24845687337985536 0.8972664260543964 >10 2.5592254037264768 21.82623111436474 >50 0.3850607818154035 10.685932373495739 >100 0.2186328801948825 15.73373838472949 >500 0.007850372717949103 2.0872265986729586 >1k 3.9251863589745515E-4 0.17656059591853204 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT 1121 0.17513408459241878 No Hit CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT 962 0.15029347848162966 No Hit CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA 862 0.13467045577044157 No Hit CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC 807 0.1260777932792881 No Hit CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG 781 0.12201580737437917 No Hit CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC 762 0.11904743305925344 No Hit CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCACTCCGC 742 0.11592282851701581 No Hit CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCAACCGATCCCGG 711 0.1110796914765475 No Hit CGACGGGCGCTGACCCCCCTCGCGGGGGGGATGCGTGCATTTATCAGATC 661 0.10326818012095344 No Hit CGGGCGCTGACCCCCCTCGCGGGGGGGATGCGTGCATTTATCAGATCAAA 659 0.10295571966672967 No Hit CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG 656 0.10248702898539404 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0012498418168950492 0.0 0.0 0.0 0.0 2 0.0012498418168950492 0.0 0.0 0.0 0.0 3 0.0015623022711188114 0.0 0.0 0.0 0.0 4 0.0017185324982306926 0.0 0.0 0.0 0.0 5 0.0018747627253425739 0.0 0.0 0.0 0.0 6 0.0018747627253425739 0.0 0.0 0.0 0.0 7 0.002030992952454455 0.0 0.0 0.0 0.0 8 0.002030992952454455 0.0 0.0 0.0 0.0 9 0.002187223179566336 0.0 0.0 0.0 0.0 10-14 0.002280961315833465 0.0 0.0 0.0 0.0 15-19 0.002562175724634851 0.0 0.0 0.0 0.0 20-24 0.0031558505876599988 0.0 0.0 0.0 0.0 25-29 0.004436938449977425 0.0 0.0 0.0 0.0 30-34 0.006780391856655643 0.0 0.0 0.0 0.0 35-39 0.009998734535160394 0.0 0.0 0.0 0.0 40-44 0.015123085984430096 0.0 0.0 0.0 0.0 45-49 0.022622136885800392 0.0 0.0 0.0 0.0 50-54 0.036745349416714444 0.0 0.0 0.0 0.0 55-59 0.05858633516695544 0.0 0.0 9.373813626712869E-5 0.0 60-64 0.08923870572630652 0.0 0.0 1.5623022711188115E-4 0.0 65-69 0.13792004449436868 0.0 0.0 3.124604542237623E-4 0.0 70-74 0.2154727292327065 0.0 0.0 3.124604542237623E-4 0.0 75-79 0.33130181961345523 0.0 0.0 3.124604542237623E-4 0.0 80-84 0.4980307179872547 0.0 0.0 3.4370649964613853E-4 0.0 85-89 0.7420310866905908 0.0 0.0 4.6869068133564346E-4 0.0 90-94 1.071114437079057 0.0 0.0 4.6869068133564346E-4 0.0 95-99 1.5535846244459686 0.0 0.0 4.6869068133564346E-4 0.0 100-104 2.257839242220906 0.0 0.0 4.6869068133564346E-4 0.0 105-109 3.2233732918177536 0.0 0.0 4.6869068133564346E-4 0.0 110-114 4.51720954066751 0.0 0.0 4.6869068133564346E-4 0.0 115-119 6.213338624330357 0.0 0.0 4.6869068133564346E-4 0.0 120-124 8.274171550163183 0.0 0.0 4.6869068133564346E-4 0.0 125-129 10.808444556235852 0.0 0.0 4.6869068133564346E-4 0.0 130-134 13.90024075077998 0.0 0.0 4.6869068133564346E-4 0.0 135-139 17.296935856555656 0.0 0.0 4.6869068133564346E-4 0.0 140 19.33567782827486 0.0 0.0 4.6869068133564346E-4 0.0 >>END_MODULE