FastQCFastQC Report
Fri 12 Jan 2024
DRR051417_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR051417_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640081
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC26110.40791712298912164TruSeq Adapter, Index 1 (100% over 50bp)
CTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT12660.19778746752364154No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC12220.19091333753071876No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA9600.1499810180274059No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT8270.1292023978215257No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC7580.11842251215080592No Hit
CCCGTCCCTCTCGCGCGCGTCACCGACTGCCAGCGACGGCCGGGTATGGG7260.11342314488322572No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG7260.11342314488322572No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC7130.11139215193077126No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCACTCCGC6850.1070177055716386No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG6420.10029980580582772No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)