##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename DRR051417_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 640081 Sequences flagged as poor quality 0 Sequence length 151 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.736698949039265 33.0 32.0 34.0 32.0 34.0 2 32.84996898829992 33.0 32.0 34.0 32.0 35.0 3 32.94504914221794 33.0 32.0 34.0 32.0 35.0 4 32.98060089269952 33.0 32.0 34.0 32.0 35.0 5 32.794007320948445 33.0 32.0 34.0 32.0 35.0 6 34.75327028922902 36.0 33.0 37.0 32.0 37.0 7 34.864023459530905 37.0 33.0 37.0 32.0 37.0 8 34.88641437568058 37.0 34.0 37.0 32.0 37.0 9 34.913823406725086 37.0 34.0 37.0 32.0 37.0 10-14 35.6695455731384 37.4 35.6 37.4 33.4 37.4 15-19 36.78754688859691 38.0 38.0 38.0 35.4 38.0 20-24 36.91622529023671 38.0 38.0 38.4 35.0 38.4 25-29 37.08804729401436 38.0 38.0 39.0 34.4 39.0 30-34 37.01258090772886 38.0 38.0 39.0 34.0 39.0 35-39 36.910727236084185 38.0 37.6 39.0 34.0 39.0 40-44 36.77541123701532 38.0 37.0 39.0 34.0 39.0 45-49 36.67413467982958 38.0 37.0 39.0 33.0 39.0 50-54 36.619719379266066 38.0 37.0 39.0 33.0 39.0 55-59 36.50076568434307 38.0 37.0 39.0 32.8 39.0 60-64 36.33376713259728 38.0 37.0 39.0 32.0 39.0 65-69 36.23397882455502 38.0 37.0 39.0 32.0 39.0 70-74 36.02375668079509 38.0 37.0 39.0 30.8 39.0 75-79 35.86716181233312 38.0 37.0 39.0 30.0 39.0 80-84 35.77343273741917 38.0 37.0 39.0 30.0 39.0 85-89 35.64193000573365 38.0 37.0 39.0 29.0 39.0 90-94 35.577550341284926 38.0 37.0 39.0 28.0 39.0 95-99 35.40603298645015 38.0 37.0 39.0 26.8 39.0 100-104 35.30191803849825 38.0 37.0 39.0 26.0 39.0 105-109 35.150730298196635 38.0 36.4 39.0 25.4 39.0 110-114 34.99919947631628 38.0 36.0 39.0 24.4 39.0 115-119 34.86394315719417 38.0 36.0 39.0 24.0 39.0 120-124 34.7197535936858 38.0 35.6 39.0 24.0 39.0 125-129 34.54332186082699 38.0 35.0 39.0 23.0 39.0 130-134 34.424088513797464 38.0 35.0 39.0 18.8 39.0 135-139 34.24741649884937 38.0 34.2 39.0 16.2 39.0 140-144 34.02979966597978 38.0 34.0 39.0 15.0 39.0 145-149 33.844568421809115 37.6 34.0 39.0 15.0 39.0 150-151 32.60334785753678 37.0 30.0 38.5 13.5 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.14641085688326427 1101 2 -0.2872446174055341 1101 3 -0.06111974663340192 1101 4 0.11337124611247873 1101 5 0.12478715675263885 1101 6 0.2216321090441653 1101 7 0.23620673126976044 1101 8 0.19179443780092953 1101 9 0.2538696209991613 1101 10-14 0.05769303343884502 1101 15-19 0.08538588504495692 1101 20-24 0.09069541553645166 1101 25-29 0.07611277400418714 1101 30-34 0.05985260372789014 1101 35-39 0.02363322291424197 1101 40-44 -0.08193384322122199 1101 45-49 -0.06116193876148657 1101 50-54 -0.19247525666737886 1101 55-59 -0.305591829071723 1101 60-64 -0.1185587010728213 1101 65-69 -0.12988877154992906 1101 70-74 -0.14018496320910856 1101 75-79 -0.23932656533946073 1101 80-84 -0.28085784592069274 1101 85-89 -0.26917658386872745 1101 90-94 -0.3226643695716547 1101 95-99 -0.33205327541203644 1101 100-104 -0.26241758877685584 1101 105-109 -0.32662007513214775 1101 110-114 -0.3381963490953126 1101 115-119 -0.26154296870618765 1101 120-124 -0.36694382954977556 1101 125-129 -0.36215568752834315 1101 130-134 -0.372550739577477 1101 135-139 -0.2032592783211058 1101 140-144 -0.23934270805174407 1101 145-149 -0.26649201154004487 1101 150-151 -0.27929101564274106 1102 1 0.050178663667743706 1102 2 0.1351559997337759 1102 3 0.1328569633166694 1102 4 0.08436695700906682 1102 5 0.15961432850222934 1102 6 0.12627942125661917 1102 7 0.0499487047015279 1102 8 0.06633029699307258 1102 9 0.1074880036958703 1102 10-14 6.998552121046941E-4 1102 15-19 -0.021832631207331588 1102 20-24 0.10316609383074393 1102 25-29 0.02518741669303637 1102 30-34 -0.004908008628888183 1102 35-39 0.06274783233264714 1102 40-44 0.061815011580385715 1102 45-49 -0.9494275559359622 1102 50-54 -0.031244464998906096 1102 55-59 0.014582392850414294 1102 60-64 -0.007671070992167017 1102 65-69 0.00733662607220964 1102 70-74 -0.01315343217279974 1102 75-79 0.060790554909054606 1102 80-84 0.09361775038757258 1102 85-89 -0.101300143509917 1102 90-94 -0.05302087191090976 1102 95-99 -0.0404744952023961 1102 100-104 -0.011965746705584479 1102 105-109 -0.14041609267352584 1102 110-114 -0.037957148385480366 1102 115-119 -0.0208209912248023 1102 120-124 -0.14093247362378492 1102 125-129 -0.14165131532286068 1102 130-134 -0.2117662930280204 1102 135-139 -0.05986970579890283 1102 140-144 -0.2301060144703584 1102 145-149 -0.1746851725911185 1102 150-151 0.3210403932020611 1103 1 0.15298123444640765 1103 2 0.12222150857554936 1103 3 0.052541378731234545 1103 4 0.11208089761797169 1103 5 0.1472859698886424 1103 6 0.14490741853533962 1103 7 0.17005252207263766 1103 8 0.23729191372753178 1103 9 -0.10992051132152625 1103 10-14 0.08738142515584002 1103 15-19 0.09300323674553113 1103 20-24 -0.02979300898318371 1103 25-29 0.08354535985029798 1103 30-34 0.017888216900040277 1103 35-39 0.03289416507505649 1103 40-44 0.004803125960471277 1103 45-49 -0.011974389703972577 1103 50-54 -0.11245490564572691 1103 55-59 -0.16352462718656824 1103 60-64 -0.32337120445489376 1103 65-69 -0.20915376941049857 1103 70-74 -0.32699640775787486 1103 75-79 -0.11215878823669811 1103 80-84 -0.3211259795568395 1103 85-89 -0.7114858446435264 1103 90-94 -0.14957223601137315 1103 95-99 -0.11595644293028329 1103 100-104 -0.024864152003658546 1103 105-109 -0.41028553513050525 1103 110-114 -0.2564332186975946 1103 115-119 -0.04566136166722856 1103 120-124 -0.078132819022251 1103 125-129 -0.17338925064747457 1103 130-134 -0.2007842117641374 1103 135-139 -0.18794223361525297 1103 140-144 -0.38834588176232643 1103 145-149 -0.3206603169437159 1103 150-151 0.03834614594760666 1104 1 0.12493941452641621 1104 2 0.09086691250013956 1104 3 0.11781096303634797 1104 4 0.10035579325327149 1104 5 0.20253937412682177 1104 6 0.07516109498191526 1104 7 0.0026035284815080217 1104 8 -0.011936647683768342 1104 9 0.0654230701134253 1104 10-14 0.09980752057686715 1104 15-19 0.1721754836842777 1104 20-24 -0.0449492457130205 1104 25-29 0.10426324960371858 1104 30-34 0.06494139770956764 1104 35-39 0.11781458222898067 1104 40-44 0.020355371461960203 1104 45-49 0.052261673770665595 1104 50-54 0.08212400974537815 1104 55-59 0.19289665994537586 1104 60-64 0.07955144263019065 1104 65-69 2.6311665612155366E-4 1104 70-74 -0.04245294478801043 1104 75-79 0.10719065547823448 1104 80-84 0.09375957314595951 1104 85-89 -0.34889500870243495 1104 90-94 -0.010583283316847769 1104 95-99 0.04543787379397202 1104 100-104 0.09875014394566506 1104 105-109 -0.016164256491457252 1104 110-114 0.05677019501343494 1104 115-119 0.15557740429234457 1104 120-124 0.043681743909061765 1104 125-129 0.1730535300592635 1104 130-134 0.21259983943718197 1104 135-139 0.06537315574075109 1104 140-144 -0.07269727448935015 1104 145-149 0.0954231704595685 1104 150-151 0.2400000297080922 1105 1 0.1002729638657982 1105 2 0.04584081176931676 1105 3 0.06665677071896425 1105 4 0.021202176952016316 1105 5 0.09090636840266342 1105 6 -0.024296009817021513 1105 7 -0.04079388664611372 1105 8 -0.013093000061637383 1105 9 0.15422819665696608 1105 10-14 -0.004353835941117268 1105 15-19 -0.026693724653554796 1105 20-24 0.05122881527267964 1105 25-29 0.02980409274732665 1105 30-34 0.020132455059169274 1105 35-39 -0.09542968461050094 1105 40-44 0.04410350840979049 1105 45-49 0.03326386983215457 1105 50-54 -0.07302348638452116 1105 55-59 -0.10969073250161898 1105 60-64 -0.0065191913475644014 1105 65-69 -0.1548562986875055 1105 70-74 -0.13382098309308077 1105 75-79 -0.09217039863128917 1105 80-84 -0.08780812674963556 1105 85-89 -0.11549190541554566 1105 90-94 -0.15279302518961657 1105 95-99 -0.1986413284236903 1105 100-104 -0.23061451026465107 1105 105-109 -0.09356404768561077 1105 110-114 -0.19387429503228049 1105 115-119 -0.20467309072151352 1105 120-124 -0.17979854553904318 1105 125-129 -0.18137862604110921 1105 130-134 -0.160060059039985 1105 135-139 -0.23461258786006312 1105 140-144 -0.34050999724559716 1105 145-149 -0.7760567589784628 1105 150-151 -0.5864767316412269 1106 1 0.13535368388472335 1106 2 0.08295127170051586 1106 3 0.03374884796265576 1106 4 0.005760719441504136 1106 5 0.04872119467716374 1106 6 -0.005702558421937454 1106 7 -0.06441290999758564 1106 8 -0.12236843096275152 1106 9 0.04122635838821509 1106 10-14 0.006276453631706147 1106 15-19 0.06859157101650482 1106 20-24 0.06416964485971022 1106 25-29 -0.0054475680647883 1106 30-34 -0.045807300687691566 1106 35-39 0.055587260223234125 1106 40-44 -0.0026370772881136872 1106 45-49 0.12911717234567277 1106 50-54 0.034747985868541775 1106 55-59 -0.005554081790876353 1106 60-64 0.16082616656882465 1106 65-69 -0.09305828984197717 1106 70-74 0.046762894743949346 1106 75-79 -0.0412927982090352 1106 80-84 -0.05421141743270397 1106 85-89 -0.12633208523826767 1106 90-94 -0.11827652627255247 1106 95-99 -0.34152409783926174 1106 100-104 -0.3658527025730436 1106 105-109 -0.1654593573701888 1106 110-114 -0.2888114480818089 1106 115-119 -0.20246076158040438 1106 120-124 -0.12011654783233183 1106 125-129 -0.2801972562578712 1106 130-134 -0.23137913517342668 1106 135-139 -0.33701988269289274 1106 140-144 -0.3004547399789672 1106 145-149 -0.3123192127942289 1106 150-151 -0.30371041018079836 1107 1 0.10613564936552677 1107 2 0.12192877887105169 1107 3 0.036340303706623445 1107 4 0.12929264718265898 1107 5 0.17581071311926877 1107 6 0.08949444253413219 1107 7 -0.1502253187934386 1107 8 -0.007750607785574459 1107 9 -0.0676223307880548 1107 10-14 0.07857303693568696 1107 15-19 0.08706990845926299 1107 20-24 0.10827118966925298 1107 25-29 0.13192424293506377 1107 30-34 0.10291307368960645 1107 35-39 0.03423668406463776 1107 40-44 -0.16493064142035507 1107 45-49 0.07143086428951051 1107 50-54 0.17936128028811282 1107 55-59 0.15208291312684707 1107 60-64 0.20258433091240846 1107 65-69 0.2219464905132824 1107 70-74 0.20407281272311195 1107 75-79 0.34565330957540397 1107 80-84 0.19892600520101666 1107 85-89 0.2681050908297635 1107 90-94 0.1603376738985247 1107 95-99 0.17806026285111898 1107 100-104 0.1869767645431395 1107 105-109 0.29922086202294906 1107 110-114 0.29184320899060623 1107 115-119 0.11308696649286532 1107 120-124 0.12442780883249327 1107 125-129 0.21710662337780917 1107 130-134 0.2605954031448974 1107 135-139 0.27835826856760804 1107 140-144 0.14906017976138486 1107 145-149 0.30199115806069443 1107 150-151 0.37843118897382766 1108 1 0.04798804249669075 1108 2 0.11685022571065673 1108 3 0.02659727606078377 1108 4 0.022466438438293324 1108 5 -0.05254758672240456 1108 6 0.028058563834825634 1108 7 0.023681635292540193 1108 8 -0.09727685306629752 1108 9 0.02944867152591968 1108 10-14 0.0713571070854897 1108 15-19 0.03280855174266151 1108 20-24 0.0695657944676924 1108 25-29 0.06893191556933687 1108 30-34 0.010020612357244829 1108 35-39 0.06672968126351009 1108 40-44 0.06210869605376246 1108 45-49 0.044759326109023334 1108 50-54 -0.023951349111477782 1108 55-59 0.04104406280411155 1108 60-64 -0.14319815599539965 1108 65-69 0.022308200414627777 1108 70-74 0.1729998618036177 1108 75-79 0.05877473205743655 1108 80-84 0.11801016060231007 1108 85-89 0.10335658273086779 1108 90-94 0.0859253002534146 1108 95-99 0.027749703844911267 1108 100-104 -0.02109289810452708 1108 105-109 0.007474424317436501 1108 110-114 -0.014982999753762272 1108 115-119 0.014504577015330256 1108 120-124 -0.022802735559181997 1108 125-129 -0.16802795219155087 1108 130-134 0.12329543968125023 1108 135-139 0.0888843920604856 1108 140-144 0.09337025161621426 1108 145-149 -0.04178304727361137 1108 150-151 0.09540841464670535 1109 1 0.11858257128236005 1109 2 0.0669357846432348 1109 3 0.13197889855907619 1109 4 0.03437976476197946 1109 5 0.0760358604544038 1109 6 0.06906771435452441 1109 7 0.09382458797453097 1109 8 0.03606559497894324 1109 9 0.04329579972252873 1109 10-14 0.05165750194782959 1109 15-19 0.07384229751671256 1109 20-24 0.029471298053721284 1109 25-29 0.09697028850644784 1109 30-34 -0.04076522410384342 1109 35-39 -0.024002139678692913 1109 40-44 0.07784542700447616 1109 45-49 0.12403833069567582 1109 50-54 0.17911242385117987 1109 55-59 0.17684227465678504 1109 60-64 0.18254974771127763 1109 65-69 0.06519085826462145 1109 70-74 0.18620320921773725 1109 75-79 0.27097969299257585 1109 80-84 0.2612852212675918 1109 85-89 0.2904128599280753 1109 90-94 0.22836603925400567 1109 95-99 0.23742214931125716 1109 100-104 0.20375503631006353 1109 105-109 0.1362987241393654 1109 110-114 0.10619434613617074 1109 115-119 0.055367675127605764 1109 120-124 0.22501846851597662 1109 125-129 -0.09587213112781257 1109 130-134 0.18002649166006535 1109 135-139 0.2162075856385215 1109 140-144 0.2867936252821366 1109 145-149 0.29534301895778725 1109 150-151 0.059533252200481 1110 1 0.00910007907099697 1110 2 0.06623119971167313 1110 3 0.08283604120980215 1110 4 -0.05713265090592046 1110 5 -0.03473677535249209 1110 6 0.04113862511137967 1110 7 0.07747554443818672 1110 8 0.2069479408548247 1110 9 0.0942310951289329 1110 10-14 0.0686365719282307 1110 15-19 0.03015944056840425 1110 20-24 0.03971525132968168 1110 25-29 -0.0018270404646330007 1110 30-34 0.10094105627472061 1110 35-39 0.1442495270051225 1110 40-44 0.11349901396670958 1110 45-49 0.1937968988259371 1110 50-54 0.08054433333052202 1110 55-59 0.04331308080055862 1110 60-64 0.0863449156060554 1110 65-69 0.09201768118623477 1110 70-74 0.1965751112159495 1110 75-79 0.09879568018903484 1110 80-84 0.027097120390394025 1110 85-89 -0.00993437527596086 1110 90-94 -0.016284596976611 1110 95-99 -0.00203496808927639 1110 100-104 0.046134925891976764 1110 105-109 0.0371550088617667 1110 110-114 -0.017961249191138506 1110 115-119 -0.01655146359181714 1110 120-124 0.07281024226563915 1110 125-129 0.05343626123551104 1110 130-134 0.02831286560880386 1110 135-139 -0.014420600323497013 1110 140-144 0.09845158894319894 1110 145-149 0.05829893787760909 1110 150-151 0.16088411906029876 1111 1 0.08898505371870868 1111 2 0.12201452004170932 1111 3 0.07841160693609339 1111 4 -1.1857255815783674E-4 1111 5 0.12678075093984376 1111 6 -0.006085709111481208 1111 7 0.13134232772041798 1111 8 0.19893393373531154 1111 9 0.19939547503726374 1111 10-14 0.11592948405409231 1111 15-19 0.056868380377210315 1111 20-24 0.05793941493601551 1111 25-29 0.01304806989775642 1111 30-34 0.05961276523937897 1111 35-39 0.06904905259181504 1111 40-44 0.2621629872113971 1111 45-49 0.17758170335406476 1111 50-54 0.14264444050420622 1111 55-59 0.15387192445121656 1111 60-64 0.2014782698109201 1111 65-69 0.150270468612959 1111 70-74 0.22619447072483467 1111 75-79 0.3163184939370822 1111 80-84 0.2826024848720081 1111 85-89 0.17232548196935227 1111 90-94 0.30131723044228664 1111 95-99 0.2316434081715073 1111 100-104 0.32361106028479014 1111 105-109 0.3589155725920037 1111 110-114 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-0.125128540027859 2112 3 0.032006967865576996 2112 4 -0.002963997641785454 2112 5 -0.12966049953767822 2112 6 -0.14430896244468983 2112 7 -0.061596187642322775 2112 8 -0.2558701958177423 2112 9 -0.07909777145670915 2112 10-14 -0.10779237762763216 2112 15-19 -0.12016826702579664 2112 20-24 -0.1200612283471969 2112 25-29 -0.11996211232325038 2112 30-34 -0.06390922192515092 2112 35-39 -0.10691119854409692 2112 40-44 -0.0257420886492028 2112 45-49 0.014679211076753518 2112 50-54 0.027412407632517954 2112 55-59 0.04334913492442638 2112 60-64 0.16652616189725222 2112 65-69 0.12966005400492975 2112 70-74 0.12170693172433289 2112 75-79 0.0012946959419437576 2112 80-84 0.2686114621949258 2112 85-89 0.2621885075447068 2112 90-94 0.12301832359798226 2112 95-99 0.1839403588465558 2112 100-104 0.2537155531651223 2112 105-109 0.2800489354188258 2112 110-114 0.3155663419322252 2112 115-119 0.29055226595194483 2112 120-124 0.2471723300966815 2112 125-129 0.31427927220457974 2112 130-134 0.06644307435303887 2112 135-139 0.22277037181535064 2112 140-144 0.42422091191459543 2112 145-149 0.470844629619414 2112 150-151 0.435176823664591 2113 1 -0.09290231834591367 2113 2 -0.08280136828081197 2113 3 -0.10009708790192207 2113 4 -0.021660081118582752 2113 5 -0.05944283914308812 2113 6 -0.01590447522606553 2113 7 0.03669513583659523 2113 8 0.037220400989944835 2113 9 0.12506361870246252 2113 10-14 0.0033569983501919864 2113 15-19 0.047630380655228066 2113 20-24 -0.028033043272820635 2113 25-29 -0.0075479604724364435 2113 30-34 -0.06649943533526681 2113 35-39 -0.06336809744053085 2113 40-44 0.13113801456285756 2113 45-49 0.09963757655811634 2113 50-54 0.15258961502323842 2113 55-59 0.17930310501228774 2113 60-64 0.2064464532635668 2113 65-69 0.20007817542816753 2113 70-74 0.25291026089990964 2113 75-79 0.10583551886301024 2113 80-84 0.32607280723449605 2113 85-89 0.25130469485205964 2113 90-94 0.3324874904238939 2113 95-99 0.33564003523837016 2113 100-104 0.17574826605783045 2113 105-109 0.022394266016966924 2113 110-114 0.1581259311140002 2113 115-119 0.323134081884632 2113 120-124 0.20433782066716333 2113 125-129 0.31551050574564243 2113 130-134 0.312347381172593 2113 135-139 0.31323951247350124 2113 140-144 0.21914920031203167 2113 145-149 0.32763718972402955 2113 150-151 0.10314183496827667 2114 1 -0.07601850261784904 2114 2 -0.07161057235330759 2114 3 -0.0468725513278585 2114 4 -0.004337363961582241 2114 5 -0.07621633425881669 2114 6 0.08560675842822718 2114 7 0.12191106264085505 2114 8 0.12462567606129227 2114 9 0.09458814480003497 2114 10-14 0.058292774292993954 2114 15-19 0.04188783563013487 2114 20-24 0.059135320267728275 2114 25-29 0.10284138398503018 2114 30-34 0.1449934327149336 2114 35-39 0.12211656720067765 2114 40-44 0.1299390914190255 2114 45-49 0.17639381721261316 2114 50-54 0.15557660512772742 2114 55-59 0.23963855904112563 2114 60-64 0.3353530728177674 2114 65-69 0.42821874134909876 2114 70-74 0.4102875351712285 2114 75-79 0.39159288970852657 2114 80-84 0.45926454922077653 2114 85-89 0.555228219651184 2114 90-94 0.4257370594592089 2114 95-99 0.5210469198795238 2114 100-104 0.45028253846561483 2114 105-109 0.5199527676884443 2114 110-114 0.560036938095152 2114 115-119 0.5926068604719603 2114 120-124 0.5210119478644515 2114 125-129 0.5544009819814661 2114 130-134 0.5293592957987343 2114 135-139 0.564605821352167 2114 140-144 0.6409641886767758 2114 145-149 0.6382715411816875 2114 150-151 0.40163945077317464 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 35.0 17 239.0 18 492.0 19 992.0 20 1627.0 21 2604.0 22 3614.0 23 4886.0 24 6273.0 25 7574.0 26 9621.0 27 11533.0 28 12159.0 29 13728.0 30 14911.0 31 16665.0 32 19130.0 33 22659.0 34 27641.0 35 37224.0 36 60975.0 37 141922.0 38 223576.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.29400810209958 16.720227596194857 10.640215847681777 41.34554845402379 2 28.435619866860602 19.248188901092206 26.06763831452582 26.24855291752138 3 25.772988106192813 21.424163504306488 21.172320378202134 31.63052801129857 4 29.42143259993657 26.466806544796672 19.3756727664155 24.736088088851254 5 30.432867090258892 25.97624363166537 20.30430523636852 23.28658404170722 6 25.104166503926844 29.121158103427536 22.098453164521363 23.676222228124253 7 24.26474149365471 20.644574671018198 29.510327599163233 25.58035623616386 8 23.499369611033604 20.312116747724115 26.38650420806117 29.802009433181116 9 24.012117216414797 18.131767698150703 28.923526866131006 28.932588219303497 10-14 26.74358401514808 22.910006702276743 22.62832360279402 27.71808567978115 15-19 26.952335303296653 22.243545958971353 22.877310884458744 27.92680785327325 20-24 27.49193850768654 22.552774653168353 22.518216472940882 27.437070366204225 25-29 27.5901018777311 22.468875033003634 22.407664030021202 27.533359059244066 30-34 27.58697727318886 22.504433032694298 22.01674475574185 27.891844938374987 35-39 27.6752785975525 21.929224582513775 22.24524708591569 28.15024973401804 40-44 27.901843672910147 22.08904810484923 22.155383459280934 27.853724762959686 45-49 28.342725373819878 22.210064038770096 21.94034817468414 27.50686241272589 50-54 28.14146959525435 22.270587628753237 21.593517070495764 27.99442570549665 55-59 28.552042632104374 21.618326430561133 21.600516184670376 28.229114752664113 60-64 28.44099418667325 22.15707074573374 21.57461321301523 27.82732185457778 65-69 28.49573725825325 21.849140968096226 21.672600811459798 27.982520962190723 70-74 28.24286301264996 21.94375399363518 21.173132775383117 28.640250218331744 75-79 28.469678056370988 21.5897987910905 21.47087634221294 28.469646810325568 80-84 28.245393942329173 21.206441059803367 21.546616756316777 29.00154824155068 85-89 28.464522458876296 21.969063290427304 21.176007411561972 28.390406839134425 90-94 28.487769516670546 21.4963731152776 21.554459513717795 28.46139785433406 95-99 28.64021897228632 21.52124496743381 21.01893354122369 28.819602519056183 100-104 28.14253196079871 21.806833822594328 21.343392476889644 28.707241739717315 105-109 27.961523619666885 21.89957208540794 21.728843693220075 28.410060601705094 110-114 28.26467275235478 22.349983830171492 21.132356686106917 28.252986731366814 115-119 27.765048486050986 22.447596476071 20.942099515530067 28.84525552234795 120-124 28.218584835356776 23.014212263760367 20.72237732411992 28.04482557676294 125-129 28.60575458418544 23.457718632485577 20.33698859988033 27.599538183448658 130-134 28.239207225335544 23.761336455854806 20.011404806579165 27.988051512230484 135-139 28.00817396548249 24.142007027235614 19.959036434451267 27.890782572830624 140-144 27.616098587522515 24.671565005054045 19.88917027688683 27.823166130536602 145-149 27.15803156163048 24.668096694012164 20.228314853901303 27.945556890456054 150-151 26.76973695516661 25.079013437361837 19.891232515884706 28.260017091586846 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 1.0 2 2.0 3 2.5 4 2.0 5 4.5 6 5.0 7 2.5 8 4.5 9 7.5 10 9.0 11 11.5 12 13.5 13 21.0 14 31.5 15 44.0 16 66.5 17 92.0 18 126.0 19 172.0 20 250.5 21 345.5 22 477.5 23 666.5 24 869.0 25 1064.5 26 1328.5 27 1692.5 28 2099.5 29 2456.0 30 2759.5 31 3124.5 32 3456.0 33 3818.0 34 4366.0 35 4879.5 36 5404.0 37 6250.5 38 7291.5 39 8073.0 40 9098.0 41 10554.0 42 11606.0 43 12677.5 44 14708.0 45 16263.5 46 17142.0 47 18785.5 48 19414.5 49 18332.0 50 17067.0 51 16652.0 52 16430.5 53 17658.0 54 19395.5 55 20469.0 56 21117.5 57 19056.5 58 17588.0 59 18920.0 60 19275.5 61 17273.0 62 15051.5 63 13020.0 64 11530.0 65 11254.0 66 13026.5 67 13542.5 68 11239.0 69 9559.5 70 7923.5 71 6539.5 72 6193.0 73 6479.0 74 8117.5 75 9127.0 76 7700.5 77 6235.5 78 6148.0 79 6044.5 80 4375.0 81 2987.5 82 3074.5 83 3313.5 84 3446.0 85 3176.5 86 2655.5 87 2511.5 88 2268.5 89 2192.0 90 2317.5 91 2156.0 92 1853.5 93 1504.0 94 1131.0 95 664.0 96 324.0 97 199.0 98 198.5 99 165.0 100 62.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 2.1872231795663362E-4 20-24 1.5623022711188115E-4 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 640081.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.48461770081465 #Duplication Level Percentage of deduplicated Percentage of total 1 82.97702408462987 29.444079775943806 2 6.305954241118086 4.475287509698122 3 2.7165649517030936 2.8918880631184884 4 1.5193476361617069 2.156538800953384 5 0.9552714538343913 1.6948721169907397 6 0.6539379845842652 1.3922843629808326 7 0.4637782362493579 1.1519895387886594 8 0.38676450477780033 1.0979352473828117 9 0.2954015971269423 0.9433991468033655 >10 2.9806302708520267 22.777983910139817 >50 0.4585599087233552 11.256631481571157 >100 0.27531214500681744 17.68251664726428 >500 0.010131486936250883 2.237571056844978 >1k 0.0013214982960327239 0.797022341519563 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC 2611 0.40791712298912164 TruSeq Adapter, Index 1 (100% over 50bp) CTAACACGTGCGCGAGTCAGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT 1266 0.19778746752364154 No Hit CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC 1222 0.19091333753071876 No Hit CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA 960 0.1499810180274059 No Hit CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT 827 0.1292023978215257 No Hit CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC 758 0.11842251215080592 No Hit CCCGTCCCTCTCGCGCGCGTCACCGACTGCCAGCGACGGCCGGGTATGGG 726 0.11342314488322572 No Hit CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG 726 0.11342314488322572 No Hit GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC 713 0.11139215193077126 No Hit CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCACTCCGC 685 0.1070177055716386 No Hit CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG 642 0.10029980580582772 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0012498418168950492 0.0 0.0 0.0 0.0 2 0.0012498418168950492 0.0 0.0 0.0 0.0 3 0.0015623022711188114 0.0 0.0 0.0 0.0 4 0.0017185324982306926 0.0 0.0 0.0 0.0 5 0.0018747627253425739 0.0 0.0 0.0 0.0 6 0.0018747627253425739 0.0 0.0 0.0 0.0 7 0.002030992952454455 0.0 0.0 0.0 0.0 8 0.002030992952454455 0.0 0.0 0.0 0.0 9 0.002187223179566336 0.0 0.0 0.0 0.0 10-14 0.0024996836337900985 0.0 0.0 0.0 0.0 15-19 0.0028746361788586135 0.0 0.0 0.0 0.0 20-24 0.003468311041883762 0.0 0.0 0.0 0.0 25-29 0.005155597494692078 0.0 0.0 0.0 0.0 30-34 0.007998987628128316 0.0 0.0 0.0 0.0 35-39 0.011717267033391087 0.0 0.0 0.0 0.0 40-44 0.017685261709064946 0.0 0.0 0.0 0.0 45-49 0.025309296792124746 0.0 0.0 0.0 0.0 50-54 0.03983870791352969 0.0 0.0 0.0 0.0 55-59 0.06192966202714969 0.0 0.0 1.5623022711188115E-4 0.0 60-64 0.09326944558579306 0.0 0.0 1.5623022711188115E-4 0.0 65-69 0.1416695699450538 0.0 0.0 1.8747627253425737E-4 0.0 70-74 0.2205970806819762 0.0 0.0 3.124604542237623E-4 0.0 75-79 0.33805096542468843 0.0 0.0 3.124604542237623E-4 0.0 80-84 0.5060921977062278 0.0 0.0 3.124604542237623E-4 0.0 85-89 0.754342028587007 0.0 0.0 3.124604542237623E-4 0.0 90-94 1.0930804070109876 0.0 0.0 3.124604542237623E-4 0.0 95-99 1.588423965091918 0.0 0.0 3.124604542237623E-4 0.0 100-104 2.3071455018974163 0.0 0.0 3.124604542237623E-4 0.0 105-109 3.2937393861089452 0.0 0.0 3.124604542237623E-4 0.0 110-114 4.622508713740918 0.0 0.0 3.124604542237623E-4 0.0 115-119 6.3641945316295905 0.0 0.0 3.124604542237623E-4 0.0 120-124 8.492081470938835 0.0 0.0 3.124604542237623E-4 0.0 125-129 11.134028349537012 0.0 0.0 3.124604542237623E-4 0.0 130-134 14.389803790457771 0.0 0.0 3.124604542237623E-4 0.0 135-139 17.98422387166624 0.0 0.0 3.124604542237623E-4 0.0 140 20.14385679312462 0.0 0.0 3.124604542237623E-4 0.0 >>END_MODULE