##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename SRR950198_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7575132 Sequences flagged as poor quality 0 Sequence length 80 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.51962262835816 39.0 38.0 40.0 35.0 40.0 2 38.01454139677038 39.0 38.0 40.0 35.0 40.0 3 38.0116693676097 39.0 38.0 40.0 35.0 40.0 4 38.04797355346415 39.0 38.0 40.0 35.0 40.0 5 38.03199601010253 39.0 38.0 40.0 35.0 40.0 6 38.152233518835054 39.0 38.0 40.0 35.0 40.0 7 38.156828290252896 39.0 38.0 40.0 35.0 40.0 8 38.09478422290199 39.0 38.0 40.0 35.0 40.0 9 38.22195269996615 39.0 38.0 40.0 35.0 40.0 10-11 38.14482381297118 39.0 38.0 40.0 35.0 40.0 12-13 38.039907951439 39.0 38.0 40.0 35.0 40.0 14-15 37.86999526872931 39.0 38.0 40.0 33.0 40.0 16-17 37.489619124788845 39.0 38.0 40.0 33.0 40.0 18-19 37.326678267784644 39.0 37.5 40.0 33.0 40.0 20-21 37.59389717565318 39.0 37.5 40.0 33.0 40.0 22-23 37.40416299016307 39.0 37.5 40.0 33.0 40.0 24-25 37.25246523756945 39.0 37.0 40.0 32.5 40.0 26-27 37.40757923426285 39.0 37.5 40.0 33.0 40.0 28-29 36.719689241058774 39.0 36.5 40.0 31.0 40.0 30-31 36.94721933558385 39.0 36.5 40.0 32.0 40.0 32-33 37.3123834673772 39.0 37.0 40.0 33.0 40.0 34-35 37.318301318049635 39.0 36.5 40.0 33.0 40.0 36-37 37.502308738646406 39.0 37.5 40.0 33.0 40.0 38-39 36.93857691192708 39.0 37.0 40.0 32.5 40.0 40-41 36.544649650989584 39.0 37.0 40.0 30.5 40.0 42-43 36.08183705577672 39.0 36.5 40.0 29.0 40.0 44-45 36.48238974581565 39.0 36.0 40.0 29.5 40.0 46-47 36.407054358920746 39.0 36.0 40.0 29.5 40.0 48-49 36.33744996126801 39.0 36.0 40.0 29.5 40.0 50-51 36.90943161122473 39.0 36.0 40.0 31.5 40.0 52-53 37.00969983097324 39.0 36.0 40.0 33.0 40.0 54-55 36.94378804224138 39.0 36.0 40.0 33.0 40.0 56-57 36.960135084114704 39.0 36.0 40.0 33.0 40.0 58-59 36.824786815596084 39.0 36.0 40.0 32.5 40.0 60-61 36.67683856862165 39.0 36.0 40.0 32.0 40.0 62-63 36.52193757151691 39.0 36.0 40.0 31.5 40.0 64-65 36.34933166841185 38.5 35.0 40.0 31.0 40.0 66-67 36.14172828935522 38.0 35.0 40.0 31.0 40.0 68-69 35.85831501022028 38.0 35.0 39.5 30.0 40.0 70-71 35.51943649298784 38.0 35.0 39.0 29.0 40.0 72-73 35.33215777626053 38.0 35.0 39.0 29.0 40.0 74-75 35.06477273267318 38.0 34.5 39.0 29.0 40.0 76-77 34.84739632259874 38.0 34.5 39.0 28.5 40.0 78-79 34.52937275548466 37.5 33.5 39.0 27.0 40.0 80 33.65818958666331 36.0 33.0 39.0 25.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.016025175849804896 1 2 0.10619364244315932 1 3 -0.14985660986273075 1 4 -0.11507599087592979 1 5 -0.321419786164455 1 6 -0.4213142959065479 1 7 -0.41216768474777865 1 8 0.030616388817342965 1 9 -0.10015209719555429 1 10-11 -0.44502537767058215 1 12-13 -0.4026665360769215 1 14-15 -0.24754273340712274 1 16-17 0.46373407551450896 1 18-19 0.4099563058035329 1 20-21 -0.33138357001072904 1 22-23 0.570886666757012 1 24-25 -0.24352672672307563 1 26-27 0.5140307494185663 1 28-29 1.1350786286229138 1 30-31 0.6797907711142983 1 32-33 0.47336055208124606 1 34-35 0.3927135027303734 1 36-37 0.47375726046811906 1 38-39 1.2829510754528215 1 40-41 1.5917747629432384 1 42-43 2.269902448225274 1 44-45 1.4102147348939909 1 46-47 1.3727172589364542 1 48-49 1.3546662747706648 1 50-51 0.5757042368327205 1 52-53 0.24529276308908976 1 54-55 0.127704032377693 1 56-57 -0.4718081797402789 1 58-59 -0.22167983011581072 1 60-61 -0.004452712221826971 1 62-63 -0.12974346675936488 1 64-65 -0.12018460398198982 1 66-67 -0.03002739951865152 1 68-69 -0.08063192846144318 1 70-71 -0.11023173877810422 1 72-73 0.11946372382124082 1 74-75 -0.1314953849685594 1 76-77 -0.06796386983143066 1 78-79 -0.09683745345447647 1 80 -0.3920713189393723 2 1 0.05420157180471108 2 2 0.14621092460593133 2 3 0.17899952147559617 2 4 0.008385452624011691 2 5 0.12672637194273761 2 6 -0.7978948110144017 2 7 -0.04090937349565138 2 8 0.122005917621415 2 9 0.017867636327004277 2 10-11 -0.3728835947257565 2 12-13 -1.3138704343004477 2 14-15 -1.3003564155603655 2 16-17 -0.7127234560047029 2 18-19 0.13355268350427707 2 20-21 -0.12075503142301613 2 22-23 0.21473539660371443 2 24-25 0.5783007737813719 2 26-27 0.27986780837715486 2 28-29 1.0454649319491338 2 30-31 0.8581974822523932 2 32-33 0.3322196409628475 2 34-35 0.2624630597986197 2 36-37 0.33650273253044816 2 38-39 1.1031497643459929 2 40-41 1.645622338440539 2 42-43 2.0748619862005313 2 44-45 1.5415443969834328 2 46-47 1.3908653025092832 2 48-49 0.04575446833767671 2 50-51 0.5706771172550873 2 52-53 0.23272430681895884 2 54-55 0.048252121887010446 2 56-57 0.12068800628419041 2 58-59 0.02941289603256081 2 60-61 -0.003332111206965749 2 62-63 0.04826588016300093 2 64-65 0.04734278382707657 2 66-67 0.023398130228919456 2 68-69 0.062142374346713325 2 70-71 -0.0036693090430830466 2 72-73 -0.05210226692479125 2 74-75 -0.028683462752880473 2 76-77 -0.4438229716899187 2 78-79 -0.28716706728346963 2 80 -0.28471736682782023 3 1 0.0527716755034362 3 2 -0.2500677602052406 3 3 0.20124534016667184 3 4 0.04592781848747762 3 5 -0.062273694344661124 3 6 -0.24647530067188228 3 7 -0.29971643809008697 3 8 -0.03286126892257357 3 9 0.026391404138941255 3 10-11 -1.0521666413245399 3 12-13 -0.5889250960205743 3 14-15 -0.8760574345185788 3 16-17 -0.7641754536472121 3 18-19 -0.08746657507566624 3 20-21 -0.8809435851915239 3 22-23 -0.8647719138877221 3 24-25 0.2569442966775739 3 26-27 0.4595262443268595 3 28-29 1.3423164364743272 3 30-31 0.9422754810389193 3 32-33 -0.45833358957443693 3 34-35 0.20497143503680348 3 36-37 0.3051413117908055 3 38-39 1.177154608419464 3 40-41 1.7056191261187266 3 42-43 2.2564888882194793 3 44-45 1.621892220090153 3 46-47 1.5130767224708777 3 48-49 1.509356177050634 3 50-51 0.6958450215461056 3 52-53 0.3133468191490323 3 54-55 0.22460942106302895 3 56-57 0.04513506184869698 3 58-59 0.006740742793049037 3 60-61 -0.0017629886212020551 3 62-63 0.07461245030317798 3 64-65 0.09517529191123231 3 66-67 0.006462978608013259 3 68-69 0.06930967485705963 3 70-71 -1.1791458803173427 3 72-73 0.04177614028800747 3 74-75 -0.29698865159957677 3 76-77 -0.06560316475374606 3 78-79 -0.1312789375810013 3 80 -0.1566480718370613 4 1 0.04143513584133274 4 2 0.2420984452853574 4 3 0.21152310479180159 4 4 0.2108412243265292 4 5 0.049454471233048025 4 6 -0.1186561089861442 4 7 0.09091758563479146 4 8 -0.1279177125292037 4 9 0.013975201474210053 4 10-11 -0.19957314514945068 4 12-13 -0.6326414984961488 4 14-15 -0.598629595277572 4 16-17 -0.8129065826594584 4 18-19 -0.24648204764088888 4 20-21 -0.33612100606115547 4 22-23 -0.0850350168278382 4 24-25 0.5032970156578074 4 26-27 0.6287856492636124 4 28-29 1.3640140842654063 4 30-31 0.8690337568014286 4 32-33 0.4162130543206928 4 34-35 0.42063262223272346 4 36-37 0.38355619335682434 4 38-39 1.2847764656075569 4 40-41 1.838229996288966 4 42-43 2.311686249022415 4 44-45 1.6493914352287575 4 46-47 1.6200370666420412 4 48-49 1.567099525626972 4 50-51 0.701333165229471 4 52-53 0.3046233066912336 4 54-55 0.28654382795445343 4 56-57 0.18834589742097307 4 58-59 0.14755277001074774 4 60-61 0.07973034152841052 4 62-63 0.05222781763680473 4 64-65 -0.12390131207226318 4 66-67 0.07697311950697383 4 68-69 0.12693267020487298 4 70-71 0.07975180704107032 4 72-73 -0.5846020161714023 4 74-75 -0.1404675198802181 4 76-77 -0.06225541247335542 4 78-79 -0.08544808436803208 4 80 -0.0832394782779673 5 1 0.05047863778554529 5 2 -0.05239651872472706 5 3 -0.29777184248779065 5 4 0.3081137229972981 5 5 0.10971428383468407 5 6 0.012412712199427745 5 7 -1.113402166150891 5 8 0.027100078090541047 5 9 -0.18010174917712618 5 10-11 -0.17584108832728162 5 12-13 0.055794453977270564 5 14-15 -0.28661022895615673 5 16-17 0.46229478475899555 5 18-19 0.18850371982966863 5 20-21 -1.2314782988625481 5 22-23 0.38134079429578094 5 24-25 0.6815033070977137 5 26-27 0.773027059801791 5 28-29 1.5397840394938314 5 30-31 0.9928200851002345 5 32-33 0.22600007566308022 5 34-35 0.4178779876888754 5 36-37 0.34712345514387266 5 38-39 1.2729844785520896 5 40-41 1.8077008143122342 5 42-43 2.3088782212519448 5 44-45 1.6307047733329014 5 46-47 1.7493974055240642 5 48-49 1.584000521848509 5 50-51 0.7218480845605271 5 52-53 0.4053403961477997 5 54-55 0.2586430951742642 5 56-57 0.08711130503043307 5 58-59 0.1284270840545787 5 60-61 0.1544300397502596 5 62-63 0.17951303007017572 5 64-65 0.1208342941250038 5 66-67 0.16461468138007973 5 68-69 0.11346918997733013 5 70-71 0.25852058228507957 5 72-73 0.3331371754731549 5 74-75 0.3324417731456748 5 76-77 0.1687677245690793 5 78-79 0.2073548350830876 5 80 0.04327159546209458 6 1 -0.17681491601751276 6 2 0.05215281109137493 6 3 -0.0307204031384245 6 4 0.10267887621782279 6 5 -0.12226616089965603 6 6 -0.1886436479696414 6 7 0.15804346328381058 6 8 -0.16321474080167775 6 9 -0.011292044085074338 6 10-11 -0.1671847796815129 6 12-13 -0.21985440598689365 6 14-15 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72-73 0.4659658626224541 7 74-75 0.3419378743692363 7 76-77 0.011014411191979434 7 78-79 0.24429142510793866 7 80 0.17799036981217853 8 1 -0.760173846836814 8 2 -0.28387691979985163 8 3 -0.25264528375929274 8 4 -0.2892121499132898 8 5 -0.24955966822234643 8 6 -0.26048945219813646 8 7 -0.21163684847196151 8 8 -0.12394619726992318 8 9 -0.32964815450336715 8 10-11 -0.21052525902000951 8 12-13 -0.17336638751360312 8 14-15 -0.3650953270988495 8 16-17 0.0355617879921013 8 18-19 0.43423629688874144 8 20-21 0.21844383317179705 8 22-23 0.6899967152881317 8 24-25 0.7579669667569817 8 26-27 0.5454994975787386 8 28-29 1.4311259245295744 8 30-31 0.9377657214934345 8 32-33 0.3080326853661006 8 34-35 0.3435300187591892 8 36-37 0.3943307125537103 8 38-39 1.2411589975065311 8 40-41 1.7896602489850366 8 42-43 2.339221016736836 8 44-45 1.6784246626097925 8 46-47 0.9987239315758316 8 48-49 1.6008362227366106 8 50-51 0.7648700863929676 8 52-53 0.3874908441148861 8 54-55 0.41655055035640487 8 56-57 0.07930527431653189 8 58-59 0.24661572772803453 8 60-61 0.29966046943884095 8 62-63 0.3056606265952695 8 64-65 0.33571006645065893 8 66-67 0.371612979526887 8 68-69 0.45605403388820065 8 70-71 0.5736860900820133 8 72-73 0.10602327042025195 8 74-75 0.5267489604336788 8 76-77 0.542349029855778 8 78-79 -0.05961138909650998 8 80 0.11289104660706073 9 1 0.07104047441546868 9 2 0.15815642896073 9 3 -0.28333219651840835 9 4 0.08429530276838904 9 5 0.20710343243157325 9 6 -0.07216871389749002 9 7 -0.168648778487956 9 8 -0.27378806474663264 9 9 0.10511317717247692 9 10-11 0.02506422179936152 9 12-13 -0.19416595350408272 9 14-15 -0.27180695039630365 9 16-17 0.5552863203075233 9 18-19 0.37460916024413393 9 20-21 0.030563114679161174 9 22-23 0.41085221962246266 9 24-25 -1.2420066573132544 9 26-27 0.23586970553052566 9 28-29 1.1619660864599766 9 30-31 0.6622594364121568 9 32-33 0.401112564310786 9 34-35 0.34303828334671493 9 36-37 0.2940199105143009 9 38-39 1.1788433132809146 9 40-41 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40-41 1.7659400218776184 96 42-43 2.3248081004845886 96 44-45 1.5201087549310301 96 46-47 1.6802447175718456 96 48-49 1.453393377534347 96 50-51 0.6100830816419318 96 52-53 -0.6803784484459001 96 54-55 0.2897017789317289 96 56-57 -0.8895870690576331 96 58-59 -0.012040603848340936 96 60-61 0.14420880469344155 96 62-63 0.10170265124327216 96 64-65 0.21906029986061526 96 66-67 0.3365289099608262 96 68-69 0.3797459362663673 96 70-71 0.4723085832242404 96 72-73 0.2573577928462427 96 74-75 0.5367203351052439 96 76-77 0.6238078698227199 96 78-79 0.7113219256360779 96 80 0.30629246723013637 97 1 0.017998191140748077 97 2 -0.1475352550866731 97 3 0.07685346678751159 97 4 0.012134097600821292 97 5 0.07446550669765628 97 6 -0.07040713848866176 97 7 0.1629176310271916 97 8 -0.13909663577069153 97 9 0.059751379202040766 97 10-11 0.03980257401226339 97 12-13 -0.0679850882614943 97 14-15 0.32547517224566747 97 16-17 0.8092071703729005 97 18-19 0.6693885482073298 97 20-21 0.32861854106092636 97 22-23 0.7880056798696273 97 24-25 0.9219332366903785 97 26-27 0.7546741886117374 97 28-29 1.5962393672598552 97 30-31 1.0396476494668576 97 32-33 0.14777177307070133 97 34-35 0.35765586741703714 97 36-37 0.42134249699745396 97 38-39 1.2834175949344555 97 40-41 1.7738155288310296 97 42-43 2.399443460699743 97 44-45 1.6373088672893985 97 46-47 1.6808244579321467 97 48-49 1.558192753997126 97 50-51 0.733415754707714 97 52-53 0.409385099625446 97 54-55 0.4036061977225529 97 56-57 0.13910322878378167 97 58-59 0.2314593833136982 97 60-61 0.210464929434373 97 62-63 0.2994201594760426 97 64-65 0.33825551519701946 97 66-67 0.349613937130826 97 68-69 0.42021135777557816 97 70-71 0.41394931586631145 97 72-73 0.38798718688626366 97 74-75 0.4096038333074006 97 76-77 0.6139197885592012 97 78-79 0.6901926661637958 97 80 0.11681188392962838 98 1 -0.27900510751597807 98 2 -1.1542649342386468 98 3 -0.35988797477729406 98 4 -0.04660854549414495 98 5 -0.17080871110497498 98 6 -0.10992017953189759 98 7 -0.036026650787142955 98 8 -0.3445566589871163 98 9 -0.2024936502995942 98 10-11 -0.007595678341139944 98 12-13 0.0976606324283864 98 14-15 0.20138382943085986 98 16-17 0.9509799115517126 98 18-19 0.720955656874267 98 20-21 0.22066326105134948 98 22-23 0.8261024357381928 98 24-25 0.8181999659007602 98 26-27 0.6501707018304259 98 28-29 1.423270819840738 98 30-31 0.9510350929832185 98 32-33 0.1007805174716907 98 34-35 -0.027016650849553514 98 36-37 0.3011285561635617 98 38-39 1.2007533683362297 98 40-41 1.6831007417448873 98 42-43 2.2613014315694215 98 44-45 1.4691375123112564 98 46-47 1.5941184053781612 98 48-49 1.5228444058543786 98 50-51 0.21398835969665697 98 52-53 -0.14785832927517362 98 54-55 0.1346692065070414 98 56-57 0.11478872703021636 98 58-59 0.058046538043392104 98 60-61 0.08434244243964173 98 62-63 0.14111960916535793 98 64-65 0.23294918360183203 98 66-67 0.38993258317657364 98 68-69 0.32483800448253675 98 70-71 0.5806021353296202 98 72-73 0.5783756857507285 98 74-75 0.5785684264093334 98 76-77 0.38215780440607716 98 78-79 0.7140997085615268 98 80 0.28492954629965084 99 1 0.060884493355999325 99 2 0.15470739678705314 99 3 -0.15582919768155534 99 4 -0.10098148309033661 99 5 0.06035410737050029 99 6 0.04479673387479011 99 7 -0.3536212273333703 99 8 -0.6744493635839888 99 9 -0.10829454573644881 99 10-11 0.07675413253326013 99 12-13 0.16000944803352013 99 14-15 0.2900103960329474 99 16-17 1.0816347929228272 99 18-19 0.8361544082970553 99 20-21 0.3256922652994234 99 22-23 0.8832978155259781 99 24-25 0.7610503324518945 99 26-27 0.6692682174452713 99 28-29 1.4476826471709927 99 30-31 0.7968613085222458 99 32-33 0.16198807265902815 99 34-35 -0.06720615372202587 99 36-37 0.22280976237614425 99 38-39 1.1189406463633915 99 40-41 1.5329088056865103 99 42-43 2.2015149302677486 99 44-45 1.2384907339227098 99 46-47 1.3672374365883044 99 48-49 1.2180301573743222 99 50-51 0.42714803870551066 99 52-53 0.1880352945252568 99 54-55 0.021010067595184978 99 56-57 -0.01973956330002835 99 58-59 -0.13686346004427463 99 60-61 -1.0094860938669044 99 62-63 -0.45853048862031187 99 64-65 -0.24087311928872168 99 66-67 -1.367937085427485 99 68-69 -2.103654182439911 99 70-71 -9.527932940122056 99 72-73 -3.46599410840755 99 74-75 -3.516956970674997 99 76-77 -0.46448776701227956 99 78-79 -0.4984916771966539 99 80 -0.6372396488641598 100 1 -0.4261591965584586 100 2 -0.9384816274450856 100 3 -0.3229878880435848 100 4 -0.33856789200951454 100 5 -0.2454994516326252 100 6 -0.10358122644851875 100 7 -0.19658100411377433 100 8 -0.5532663126585717 100 9 -0.2293500732334195 100 10-11 -0.12512945004467468 100 12-13 0.022530083358262232 100 14-15 0.1990826488131816 100 16-17 0.9181327540292017 100 18-19 0.6393653492727509 100 20-21 0.15309747458947953 100 22-23 0.7083094218224488 100 24-25 0.5139148910264453 100 26-27 0.35514195969182083 100 28-29 1.315492084197892 100 30-31 0.8173451276148285 100 32-33 0.09828430975127844 100 34-35 0.1631318821630785 100 36-37 -0.06169598378281904 100 38-39 0.8532103695772335 100 40-41 1.3159246228368175 100 42-43 2.0018773848049847 100 44-45 1.240037117740954 100 46-47 1.2133613321271568 100 48-49 1.255829118575278 100 50-51 0.4368921663287537 100 52-53 0.274728144597681 100 54-55 0.1691157254665825 100 56-57 0.04321373392808425 100 58-59 -0.26428766881338817 100 60-61 -0.061513888935067484 100 62-63 -0.2327028973025591 100 64-65 0.01259068945233821 100 66-67 0.2540191077781344 100 68-69 0.3106842488884567 100 70-71 0.41072264073593345 100 72-73 0.1456745433651676 100 74-75 0.35421868172316806 100 76-77 0.09511566184853137 100 78-79 -0.5330913289146082 100 80 -1.2406671964229403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2592.0 5 207.0 6 239.0 7 468.0 8 724.0 9 1270.0 10 1836.0 11 2998.0 12 4159.0 13 5502.0 14 6085.0 15 5911.0 16 6259.0 17 6693.0 18 7509.0 19 8351.0 20 9943.0 21 11451.0 22 13954.0 23 17440.0 24 21663.0 25 27965.0 26 36701.0 27 50842.0 28 70328.0 29 98326.0 30 131544.0 31 168851.0 32 211956.0 33 261404.0 34 325640.0 35 427696.0 36 603236.0 37 988572.0 38 1513228.0 39 2523582.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.64107744587932 13.963306561194422 11.538416844077975 48.85719914884829 2 25.21130886414068 21.70206423747226 33.780296338677154 19.30633055970991 3 23.297285073786924 27.2072809906849 23.69290901283055 25.80252492269762 4 26.134487117289268 31.845440474186727 18.227515899182517 23.79255650934149 5 26.408477106405538 33.48230763503527 21.100899627887674 19.008315630671518 6 19.077819370012296 35.7654361666569 22.997579448120508 22.1591650152103 7 18.377145111134695 15.318742960518708 41.943902759714284 24.36020916863231 8 20.237495531431005 20.442534862758826 27.350704911808798 31.96926469400137 9 21.99643517763123 19.663802558160043 30.630410136747454 27.70935212746128 10-11 24.68722355408783 29.269909172097858 20.504920568080856 25.537946705733454 12-13 23.03111078489432 23.20916222687668 27.374813499777822 26.38491348845118 14-15 23.412931946268394 24.805495138566563 26.367395314035452 25.41417760112959 16-17 24.556479500217094 24.819498123398294 24.734938507317832 25.889083869066788 18-19 24.124785654687265 24.97241786133163 24.834063279548136 26.06873320443297 20-21 24.0827476393725 25.612291007681883 25.289218334979868 25.015743017965757 22-23 23.90090364101906 25.369293894812657 25.069959176949 25.659843287219285 24-25 23.775527857173174 25.49998636987358 24.986232912260334 25.73825286069291 26-27 23.98339577398699 25.67579172150397 25.372150542183856 24.968661962325182 28-29 24.17852904681897 24.54620595477908 24.78863297353336 26.48663202486859 30-31 23.830703411875508 25.09442241146592 25.169998650565837 25.904875526092724 32-33 23.923700441587055 25.9635843316693 25.37024937150692 24.742465855236727 34-35 24.212871434182343 25.659529805499353 25.155141741561355 24.972457018756952 36-37 24.056727658776502 25.710183178306888 25.224480371923537 25.008608790993076 38-39 24.216840119848744 24.96977278813611 25.19725990992796 25.616127182087194 40-41 24.13889868994451 24.64562555141252 24.853030924650106 26.362444833992853 42-43 24.007628427352486 24.675947638271847 25.03164867828148 26.284775256094186 44-45 24.042766200864012 25.193424744168034 25.157972924663497 25.605836130304453 46-47 24.306331492036616 24.951969330090005 24.95365948149852 25.788039696374867 48-49 23.932634318705013 25.13803942327365 25.06886373954979 25.86046251847155 50-51 24.248984191478858 25.830205936576476 25.276881624438204 24.643928247506462 52-53 24.33724587241516 25.52654528000304 25.250609494329602 24.885599353252193 54-55 24.145567412411893 25.549701823579383 25.340979319231437 24.96375144477728 56-57 24.203094836419023 25.66892998067419 25.3981217281379 24.72985345476889 58-59 24.411090692676883 25.54728954031329 25.352711931609328 24.688907835400503 60-61 24.113147177593483 25.49288676803888 25.42449868770425 24.969467366663384 62-63 24.255128491490314 25.70014621527387 25.415946547202083 24.628778746033735 64-65 24.399958970876582 25.605420491090094 25.21372965424779 24.780890883785535 66-67 24.34101925558603 25.466657223480034 25.274099383026805 24.918224137907135 68-69 24.37621210775101 25.70042683090233 25.329905185798108 24.593455875548557 70-71 24.393763065323082 25.536365894954898 25.283311216309514 24.786559823412503 72-73 24.331844183239173 25.520815031600296 25.32892920429503 24.8184115808655 74-75 24.35678348575312 25.756257448715086 25.340838945116733 24.546120120415065 76-77 24.58692703157164 25.583200965342773 25.298997387983803 24.530874615101787 78-79 24.309710908125275 25.613894166109585 25.41950161311205 24.656893312653086 80 24.72288271676322 25.686007847784037 25.202372711128994 24.38873672432375 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 12.0 1 11.0 2 8.0 3 10.0 4 14.0 5 14.0 6 21.0 7 38.0 8 60.5 9 83.0 10 93.0 11 110.5 12 153.0 13 235.0 14 379.5 15 467.0 16 550.5 17 781.0 18 1059.0 19 1436.5 20 1683.0 21 2062.5 22 3056.5 23 4293.5 24 6077.0 25 7238.0 26 8812.5 27 12465.5 28 17321.0 29 25177.5 30 30257.0 31 35485.5 32 46588.5 33 59964.0 34 76951.5 35 86438.0 36 100634.0 37 127100.0 38 155513.5 39 189126.5 40 206596.0 41 227898.0 42 268447.0 43 313654.5 44 363500.0 45 387385.0 46 407476.0 47 445597.5 48 477781.0 49 496603.5 50 501273.0 51 498863.0 52 489945.5 53 466824.5 54 428907.0 55 407603.0 56 381102.0 57 325398.0 58 270269.0 59 220001.5 60 195660.0 61 172421.0 62 129264.5 63 95620.5 64 69328.0 65 56762.0 66 47691.5 67 32887.0 68 22904.0 69 15838.0 70 13021.0 71 11423.5 72 7765.0 73 4922.0 74 3235.5 75 2331.0 76 1688.0 77 836.0 78 496.0 79 298.0 80 231.0 81 193.0 82 133.5 83 90.5 84 65.5 85 62.0 86 49.0 87 28.0 88 17.5 89 14.5 90 14.0 91 14.0 92 11.0 93 8.0 94 10.0 95 12.0 96 9.0 97 4.5 98 4.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030811344277565066 2 0.0010956904777368896 3 1.1880981083893985E-4 4 7.524621353132856E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.009933820295144693 12-13 9.7688066689795E-4 14-15 0.0 16-17 3.960327027964661E-5 18-19 4.026332478430739E-4 20-21 0.015042642161219104 22-23 0.0 24-25 6.600545046607769E-6 26-27 9.570790317581264E-4 28-29 0.003630299775634273 30-31 0.06986676931834324 32-33 9.042746713852644E-4 34-35 0.008013061686581831 36-37 3.4982888747021174E-4 38-39 0.005511455113917487 40-41 0.011016309682788366 42-43 0.01790067816640027 44-45 0.056678880315220906 46-47 0.02436921231207588 48-49 0.021933611189877616 50-51 1.1220926579233207E-4 52-53 0.0 54-55 3.300272523303884E-5 56-57 4.026332478430739E-4 58-59 7.260599551268546E-5 60-61 0.038362367810884354 62-63 0.0 64-65 3.3002725233038845E-4 66-67 7.260599551268546E-5 68-69 1.3201090093215538E-5 70-71 0.05334560506668398 72-73 0.06951694043087302 74-75 0.0 76-77 0.024600231388707153 78-79 0.07804484463109027 80 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 80 7575132.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.88410552430732 #Duplication Level Percentage of deduplicated Percentage of total 1 76.20454279199444 42.58622710819411 2 12.531564717222713 14.006305700839208 3 4.443992360699797 7.4504561410368915 4 2.1277172361579844 4.756222982053613 5 1.2054703807182956 3.3683316981244076 6 0.7606084486197323 2.5503553683206883 7 0.5049592334859012 1.975343656271961 8 0.37959734012351815 1.6970766249767253 9 0.28169200366336733 1.4167895092269467 >10 1.460759871513752 14.365306839391897 >50 0.07131121314489208 2.7184572153781006 >100 0.026524267174301095 2.5200177211357233 >500 9.510456462766972E-4 0.3540874965523573 >1k 3.090898350399266E-4 0.23502193849743314 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.05323999634593826 0.0 0.0 0.0 0.0 2 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