##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename SRR950197_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8243759 Sequences flagged as poor quality 0 Sequence length 80 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.58059230018733 39.0 38.0 40.0 36.0 40.0 2 38.09639122152892 39.0 38.0 40.0 35.0 40.0 3 38.09396417338255 39.0 38.0 40.0 35.0 40.0 4 38.130272731165476 39.0 38.0 40.0 35.0 40.0 5 38.11658188940264 39.0 38.0 40.0 35.0 40.0 6 38.22857691497289 39.0 38.0 40.0 35.0 40.0 7 38.2326991849228 39.0 38.0 40.0 35.0 40.0 8 38.17403965836459 39.0 38.0 40.0 35.0 40.0 9 38.29074527773071 39.0 38.0 40.0 35.0 40.0 10-11 38.21886308175675 39.0 38.0 40.0 35.0 40.0 12-13 38.11784132699658 39.0 38.0 40.0 35.0 40.0 14-15 37.95844147069316 39.0 38.0 40.0 34.5 40.0 16-17 37.57683964317734 39.0 38.0 40.0 33.0 40.0 18-19 37.42357394242117 39.0 37.5 40.0 33.0 40.0 20-21 37.683973718785325 39.0 38.0 40.0 33.0 40.0 22-23 37.48530045577509 39.0 38.0 40.0 33.0 40.0 24-25 37.33763693237515 39.0 37.5 40.0 32.5 40.0 26-27 37.49075215566103 39.0 38.0 40.0 33.0 40.0 28-29 36.809354567497664 39.0 37.0 40.0 31.5 40.0 30-31 37.033270380660085 39.0 37.0 40.0 32.0 40.0 32-33 37.398174667648576 39.0 37.0 40.0 33.0 40.0 34-35 37.406447471353786 39.0 37.0 40.0 33.0 40.0 36-37 37.59104287255364 39.0 38.0 40.0 33.0 40.0 38-39 37.036067102398306 39.0 38.0 40.0 32.5 40.0 40-41 36.64048851986091 39.0 37.5 40.0 31.0 40.0 42-43 36.17642764665973 39.0 37.0 40.0 29.5 40.0 44-45 36.58455293270946 39.0 37.0 40.0 29.5 40.0 46-47 36.50561643056281 39.0 36.0 40.0 30.5 40.0 48-49 36.43991327257383 39.0 36.0 40.0 30.0 40.0 50-51 37.012911828208466 39.0 36.5 40.0 32.0 40.0 52-53 37.11676869738672 39.0 36.5 40.0 33.0 40.0 54-55 37.054109235847385 39.0 36.0 40.0 33.0 40.0 56-57 37.07576610378833 39.0 36.5 40.0 33.0 40.0 58-59 36.94315505826893 39.0 36.0 40.0 33.0 40.0 60-61 36.80030529761969 39.0 36.0 40.0 33.0 40.0 62-63 36.65226276022868 39.0 36.0 40.0 32.0 40.0 64-65 36.48122137000851 39.0 36.0 40.0 31.0 40.0 66-67 36.2845809175159 38.5 35.5 40.0 31.0 40.0 68-69 36.006666194390206 38.0 35.0 40.0 30.5 40.0 70-71 35.67598440226115 38.0 35.0 40.0 29.5 40.0 72-73 35.50695611067718 38.0 35.0 39.5 29.0 40.0 74-75 35.24676503764848 38.0 35.0 39.0 29.0 40.0 76-77 35.04099009929815 38.0 35.0 39.0 29.0 40.0 78-79 34.73865835961483 38.0 34.0 39.0 28.5 40.0 80 33.866471472540624 37.0 33.0 39.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.054813359161080655 1 2 0.04349432125513175 1 3 -0.21332670953295718 1 4 -0.16229123000150025 1 5 -0.35092234246975806 1 6 -0.4564614606647197 1 7 -0.4273009807196786 1 8 -0.062221293771635544 1 9 -0.13284964335664995 1 10-11 -0.4954591973692146 1 12-13 -0.41293651902553563 1 14-15 -0.26660294997263634 1 16-17 0.4633865018688823 1 18-19 0.39412001070189717 1 20-21 -0.3005088184095115 1 22-23 0.5676815974399005 1 24-25 -0.24827218314112542 1 26-27 0.5168342724567268 1 28-29 1.0841618290411361 1 30-31 0.6664580646334741 1 32-33 0.32901303832792905 1 34-35 0.33788909213124896 1 36-37 0.3287049813060463 1 38-39 1.1515443386305932 1 40-41 1.5140515891009088 1 42-43 2.1590504408849043 1 44-45 1.3048388195700298 1 46-47 1.3055334981106057 1 48-49 1.2159457580492656 1 50-51 0.4640505384047202 1 52-53 0.11205371352929205 1 54-55 0.03630353975553646 1 56-57 -0.45627993562197133 1 58-59 -0.29536810828818716 1 60-61 -0.026150968363126026 1 62-63 -0.1482754069438954 1 64-65 -0.08828474320220892 1 66-67 -0.05338462375996045 1 68-69 -0.08555789588667295 1 70-71 -0.22204933742256827 1 72-73 -0.008251249827758045 1 74-75 -0.14742640013661656 1 76-77 -0.1199535342294169 1 78-79 -0.1286066086954989 1 80 -0.4869858879948481 2 1 -0.05389587707302468 2 2 0.04729718262971261 2 3 0.07246009869633241 2 4 -0.08908309561703476 2 5 -0.01230048458248234 2 6 -0.7875030659094691 2 7 -0.12222248568896532 2 8 -0.006502400011633824 2 9 -0.06055975865249508 2 10-11 -0.41925954662369236 2 12-13 -1.3142500438266183 2 14-15 -1.3548864915735166 2 16-17 -0.7636696946068255 2 18-19 0.08695708401218383 2 20-21 -0.18682104353733564 2 22-23 0.15797473045492438 2 24-25 0.5432720554684138 2 26-27 0.24355663608263 2 28-29 1.0314426858136514 2 30-31 0.8195889862151944 2 32-33 0.2157889938494506 2 34-35 0.19066856113185793 2 36-37 0.20741075098300854 2 38-39 0.9794999438858127 2 40-41 1.5816037548450126 2 42-43 2.000046042930194 2 44-45 1.4446517015125977 2 46-47 1.2699223812813258 2 48-49 0.013854418059978002 2 50-51 0.48141951127905713 2 52-53 0.1441612252917679 2 54-55 0.04205050296867796 2 56-57 0.03640812011918371 2 58-59 0.002573907095055006 2 60-61 -0.0966870094362946 2 62-63 -0.06288254799419946 2 64-65 -0.04469879642077501 2 66-67 -0.12434427108620838 2 68-69 -0.0014932157720295436 2 70-71 -0.05362703607631403 2 72-73 -0.19395958919425027 2 74-75 -0.12192904396857074 2 76-77 -0.5301787353298621 2 78-79 -0.4215532034643559 2 80 -0.45284582683170527 3 1 0.0699884273520226 3 2 -0.22829219184904304 3 3 0.16857916480046953 3 4 0.06192518234503552 3 5 -0.022859459480685018 3 6 -0.25720524940741996 3 7 -0.31231514643367575 3 8 -0.023360231378589447 3 9 0.034758374650493806 3 10-11 -1.0402772087727925 3 12-13 -0.6586771835004868 3 14-15 -0.8933973802553083 3 16-17 -0.6007432830536885 3 18-19 -0.015909918363028908 3 20-21 -0.7766164423328803 3 22-23 -0.7248807991574822 3 24-25 0.34560773136754364 3 26-27 0.5558999388843375 3 28-29 1.4322069605088998 3 30-31 1.0273436880313938 3 32-33 -0.3380236292144829 3 34-35 0.21145601181626006 3 36-37 0.32619118837398986 3 38-39 1.2019919964948684 3 40-41 1.7388807129568278 3 42-43 2.3248474841915225 3 44-45 1.68493237008731 3 46-47 1.6112765057863712 3 48-49 1.6561918296375993 3 50-51 0.8176056710795905 3 52-53 0.4348305130010033 3 54-55 0.36014873228853617 3 56-57 0.19520707381474267 3 58-59 0.19324791210475922 3 60-61 0.11779153800966924 3 62-63 0.14632220157115938 3 64-65 0.11426022140125269 3 66-67 0.02945920545620595 3 68-69 0.07086297138165065 3 70-71 -1.07006063449073 3 72-73 0.02130602723088515 3 74-75 -0.2536931486969678 3 76-77 0.0214888123796797 3 78-79 -0.1450964938786612 3 80 -0.10252663483685609 4 1 0.03290273127590382 4 2 0.2745014165820052 4 3 0.19453225232319937 4 4 0.20199070844593336 4 5 0.06260757403351391 4 6 -0.11342980220529597 4 7 0.12259891279872903 4 8 -0.09154977053349 4 9 0.0317742767511362 4 10-11 -0.18900417291980176 4 12-13 -0.5770308224326044 4 14-15 -0.615141774402062 4 16-17 -0.710495803233627 4 18-19 -0.22831983064398287 4 20-21 -0.359174421408909 4 22-23 -0.1171757206712698 4 24-25 0.47535339449567005 4 26-27 0.5859162563914424 4 28-29 1.3425327985060775 4 30-31 0.8287460404838569 4 32-33 0.3797232602162737 4 34-35 0.3536075327444905 4 36-37 0.3550640000213079 4 38-39 1.2258167661007775 4 40-41 1.7764748551567138 4 42-43 2.297124295690878 4 44-45 1.6391973282763672 4 46-47 1.6237868553821997 4 48-49 1.5462692346560516 4 50-51 0.7390723688543233 4 52-53 0.33650088797646305 4 54-55 0.33406063766691574 4 56-57 0.14264108536076492 4 58-59 0.0561992042749182 4 60-61 0.03010115523822776 4 62-63 0.017018761379723912 4 64-65 -0.1684721714822075 4 66-67 0.07365098382263113 4 68-69 0.147220653413612 4 70-71 0.11190467907002244 4 72-73 -0.4064133913138832 4 74-75 -0.07198860227244808 4 76-77 0.05129436801427545 4 78-79 -0.04141089966248046 4 80 -4.7079965648322286E-4 5 1 0.006123656741642947 5 2 -0.07735384705316051 5 3 -0.3558449461763544 5 4 0.2184708917514726 5 5 0.08919899034663814 5 6 -0.035471088382713845 5 7 -1.1536367262486849 5 8 -0.021645714392064974 5 9 -0.1999199101556286 5 10-11 -0.2016594340911766 5 12-13 0.00841119069799845 5 14-15 -0.3771141225320136 5 16-17 0.39949059804699516 5 18-19 0.11303115798359897 5 20-21 -1.2343495725038949 5 22-23 0.42091900087664413 5 24-25 0.6238053168289852 5 26-27 0.7214876524057274 5 28-29 1.5230147352046188 5 30-31 0.9939149431486456 5 32-33 0.2782062878236573 5 34-35 0.4148191190997821 5 36-37 0.31079455648747256 5 38-39 1.2561181963958745 5 40-41 1.8047460589273996 5 42-43 2.288749448736354 5 44-45 1.6638879009487582 5 46-47 1.6701992777815562 5 48-49 1.5423626312356404 5 50-51 0.6709529136517318 5 52-53 0.35239362987386613 5 54-55 0.23803311309237785 5 56-57 0.09033956883321537 5 58-59 0.06982102163049575 5 60-61 0.08307182867938678 5 62-63 0.0921889506873157 5 64-65 0.03723419199301503 5 66-67 0.13336062002438354 5 68-69 0.0023533658277301583 5 70-71 0.19666997283862742 5 72-73 0.24625536591921104 5 74-75 0.23936420681781811 5 76-77 0.0328969500599996 5 78-79 0.1475562409860487 5 80 0.030780727376367167 6 1 -0.13265988829320463 6 2 -0.021300110239884873 6 3 -0.06418522603989629 6 4 0.05097643983101108 6 5 -0.12542951490772936 6 6 -0.182619149931611 6 7 0.10739460080750263 6 8 -0.1958436119947251 6 9 -0.07556746834357142 6 10-11 -0.1446532334916455 6 12-13 -0.221490414461897 6 14-15 -0.2739203578946814 6 16-17 0.3939006090164838 6 18-19 0.3513798590332584 6 20-21 0.20911227604128868 6 22-23 0.3411902562768887 6 24-25 0.8036979060632774 6 26-27 0.6166342191128109 6 28-29 1.5064205143113227 6 30-31 1.0294947457411965 6 32-33 0.37105606272356084 6 34-35 0.4150483783050021 6 36-37 0.4182024365554753 6 38-39 1.290849912660974 6 40-41 1.8134878489144555 6 42-43 2.3560992055502226 6 44-45 1.6599598836652731 6 46-47 1.68573406306502 6 48-49 1.564880755549197 6 50-51 0.6731587672160302 6 52-53 0.4235267833764027 6 54-55 0.34997228492508015 6 56-57 0.21911972425937876 6 58-59 0.14479889501818377 6 60-61 0.09300072005380855 6 62-63 -0.25397592789980195 6 64-65 -0.07127818299144906 6 66-67 0.217846433861709 6 68-69 0.2262082653770463 6 70-71 0.2856404069700105 6 72-73 0.44351092087414656 6 74-75 0.17389853459894056 6 76-77 0.12808993083338294 6 78-79 0.2304839525002791 6 80 -0.02600867103311799 7 1 0.06389994922964348 7 2 0.22395354429500003 7 3 0.2537325897844056 7 4 0.22007400913793163 7 5 0.12554208044628012 7 6 -0.001310324868427415 7 7 0.03824767192575251 7 8 0.16317217549280372 7 9 0.001212946189809827 7 10-11 -0.05390218129606694 7 12-13 -0.2812867506015806 7 14-15 -0.18969760113179746 7 16-17 0.5016186523590918 7 18-19 0.4339528098895258 7 20-21 0.025859776668767154 7 22-23 0.6377521168652649 7 24-25 0.85748251358131 7 26-27 0.3221622013330432 7 28-29 1.1772702115043572 7 30-31 0.9270963975298656 7 32-33 0.37588413681193344 7 34-35 0.36934668326625797 7 36-37 0.42153682736369547 7 38-39 1.1591691820618877 7 40-41 1.7667848976406688 7 42-43 2.2320108544904826 7 44-45 1.6097899945212077 7 46-47 1.2033297762294666 7 48-49 1.551812235851223 7 50-51 0.6722013172006598 7 52-53 0.32669492817050383 7 54-55 0.34131304057149237 7 56-57 0.11403823501878207 7 58-59 0.06975138521840307 7 60-61 -0.03115859949978983 7 62-63 0.1329758555976852 7 64-65 0.1737622033608659 7 66-67 0.11585221515429822 7 68-69 0.18203088170210435 7 70-71 0.3259203669987514 7 72-73 0.31320756743750877 7 74-75 0.2011932752572534 7 76-77 -0.05950087538966642 7 78-79 0.2032620341408844 7 80 0.12614745739656286 8 1 -0.7351155214953593 8 2 -0.3287248177630886 8 3 -0.23140983181792762 8 4 -0.2769944546476282 8 5 -0.20365124647776156 8 6 -0.28885651180947036 8 7 -0.22583521696802933 8 8 -0.17922503377278787 8 9 -0.27282846400893845 8 10-11 -0.18070217458623006 8 12-13 -0.15238652487835225 8 14-15 -0.30439769049149845 8 16-17 0.09034807769971565 8 18-19 0.44719024143255126 8 20-21 0.25022713832810695 8 22-23 0.7061953710120008 8 24-25 0.8242702020028858 8 26-27 0.6250474501335788 8 28-29 1.4608047352052935 8 30-31 0.9831980012865387 8 32-33 0.38446521876668527 8 34-35 0.37379081668164105 8 36-37 0.3872919167950357 8 38-39 1.2414549765261995 8 40-41 1.7659380424065247 8 42-43 2.34110499459824 8 44-45 1.6967036566996185 8 46-47 0.9968609135578816 8 48-49 1.6564392648352921 8 50-51 0.7576489491687113 8 52-53 0.4311771014877763 8 54-55 0.4869488136656628 8 56-57 0.12919959095169986 8 58-59 0.2788979809005525 8 60-61 0.2954714461166361 8 62-63 0.37454216297337695 8 64-65 0.4425648708019594 8 66-67 0.5127237171695214 8 68-69 0.5321015097606292 8 70-71 0.6694048674424025 8 72-73 0.20478624608124107 8 74-75 0.6056373737255569 8 76-77 0.5245964012189575 8 78-79 -0.04769123496669181 8 80 0.03567557040425129 9 1 0.15352156547916707 9 2 0.2129882856553209 9 3 -0.1605823870887093 9 4 0.18756964430798462 9 5 0.27318254738906944 9 6 0.042608647771480435 9 7 -0.10372429109272474 9 8 -0.1247879581650082 9 9 0.13799363564491784 9 10-11 0.09830111770956051 9 12-13 -0.0993652853080178 9 14-15 -0.1650533011968065 9 16-17 0.6697460463004461 9 18-19 0.4659589931747803 9 20-21 0.10527274061661274 9 22-23 0.5410744911178682 9 24-25 -0.9910940817976055 9 26-27 0.4252716658959983 9 28-29 1.3139659277930988 9 30-31 0.7589751143504486 9 32-33 0.49922002189200043 9 34-35 0.5038402958412576 9 36-37 0.40887053405786844 9 38-39 1.3224987817868339 9 40-41 1.8762481041387602 9 42-43 2.474103636001871 9 44-45 1.627402244517178 9 46-47 1.7743497362610512 9 48-49 1.6699266964656871 9 50-51 0.830541365656984 9 52-53 0.5226086818168199 9 54-55 0.489086735554487 9 56-57 0.18911664079486457 9 58-59 0.1687378045536576 9 60-61 0.13308997823715885 9 62-63 0.19419356257120768 9 64-65 0.11711933509008787 9 66-67 0.20953803872384213 9 68-69 0.25389242123806355 9 70-71 0.2427479414426017 9 72-73 0.19573701996696258 9 74-75 0.18479705345405506 9 76-77 0.20462806571602243 9 78-79 0.31259369137951865 9 80 0.28973306447053915 10 1 -0.36534916390161953 10 2 -0.10384363029593402 10 3 -0.050025107387504875 10 4 0.003893183598350447 10 5 -0.0681428056129576 10 6 0.0065008058196553975 10 7 -0.03792491447213564 10 8 -0.02735825628545996 10 9 -0.061895996641837314 10 10-11 -0.1430516760781515 10 12-13 -0.08279958192376569 10 14-15 -0.5400017447635008 10 16-17 0.37633465619121864 10 18-19 0.34035684090360974 10 20-21 -0.015650304269392734 10 22-23 0.3488304595727243 10 24-25 0.6888780071951786 10 26-27 0.5645877958383636 10 28-29 1.420647070565792 10 30-31 1.0389850225900688 10 32-33 0.463457725598694 10 34-35 0.44345958432889176 10 36-37 0.4834827020478727 10 38-39 1.4291096264390717 10 40-41 1.9346072660696265 10 42-43 2.5071836542733337 10 44-45 1.7916031701818653 10 46-47 1.6445383958609625 10 48-49 1.7863723925950907 10 50-51 0.7358075110494866 10 52-53 0.5941187311229044 10 54-55 0.47573217507727605 10 56-57 0.3926025946056768 10 58-59 0.25859747701076685 10 60-61 -0.3007454173535109 10 62-63 0.3126147265710486 10 64-65 -0.540979895090075 10 66-67 0.38062660159246064 10 68-69 0.5165186757219544 10 70-71 0.6739774028374157 10 72-73 0.5398849113592235 10 74-75 0.7537341176594481 10 76-77 0.7005621272209979 10 78-79 0.7462663903168192 10 80 0.4430611581924069 11 1 -0.004457137938750577 11 2 0.2844110100611772 11 3 0.2790897770573437 11 4 0.19869741038106525 11 5 0.12721158696287205 11 6 0.07238702644000483 11 7 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42-43 2.331992912061061 96 44-45 1.5339378021086176 96 46-47 1.6544256294373483 96 48-49 1.4439990521528117 96 50-51 0.5738882514220336 96 52-53 -0.674543328276684 96 54-55 0.24443266016042742 96 56-57 -0.8156864804568187 96 58-59 0.013682253264036603 96 60-61 0.1542275087201972 96 62-63 0.13909360069983734 96 64-65 0.23758275676356533 96 66-67 0.37951348018183495 96 68-69 0.43490643086023795 96 70-71 0.6316547156284642 96 72-73 0.43136949178752104 96 74-75 0.632115743894623 96 76-77 0.7133496711429999 96 78-79 0.7972375991400185 96 80 0.32034799447538376 97 1 -0.04645155826244718 97 2 -0.21395618591020593 97 3 -0.06516961156417977 97 4 -0.06136696888763993 97 5 -0.059634485588112796 97 6 -0.1345539080722915 97 7 0.03024543914194311 97 8 -0.24866876057370746 97 9 -0.06935611723072554 97 10-11 -0.08258042224595386 97 12-13 -0.17727370020250532 97 14-15 0.19945351220517438 97 16-17 0.6827190328240533 97 18-19 0.5226222865653654 97 20-21 0.18563553711966563 97 22-23 0.6946992895789208 97 24-25 0.8160157572614963 97 26-27 0.6547959745942933 97 28-29 1.454279899753125 97 30-31 0.9369156897161162 97 32-33 0.10086773312198005 97 34-35 0.25223523434384276 97 36-37 0.3353596067543876 97 38-39 1.2061885608922296 97 40-41 1.7820122963073075 97 42-43 2.3449050551527506 97 44-45 1.598881091833114 97 46-47 1.675117913029517 97 48-49 1.513674852598676 97 50-51 0.616873990030598 97 52-53 0.28835318847848157 97 54-55 0.2790327191569375 97 56-57 0.05803942663713002 97 58-59 0.12380965929256149 97 60-61 0.15941047728850322 97 62-63 0.12270555859188903 97 64-65 0.20059353678473713 97 66-67 0.28259608721985074 97 68-69 0.34214518736843047 97 70-71 0.35649206019951407 97 72-73 0.33611600555643406 97 74-75 0.3852829305542187 97 76-77 0.4320449630584804 97 78-79 0.5555098907572926 97 80 -0.01537273853189447 98 1 -0.301050586499521 98 2 -1.2025196259536344 98 3 -0.3860338699232031 98 4 -0.16614833815277308 98 5 -0.25947381611330655 98 6 -0.17230586684631533 98 7 -0.08851689429105392 98 8 -0.43573087666964483 98 9 -0.18552289819655954 98 10-11 -0.046647006770633936 98 12-13 0.0738759477028168 98 14-15 0.16910203058544226 98 16-17 0.9241428584957987 98 18-19 0.7311654951171533 98 20-21 0.2114458485155808 98 22-23 0.807750457302717 98 24-25 0.8180007153851605 98 26-27 0.6148871659117887 98 28-29 1.462506543877403 98 30-31 1.0153717528378223 98 32-33 0.1424453579906313 98 34-35 0.013356255920008664 98 36-37 0.40781662668106833 98 38-39 1.2349369048984187 98 40-41 1.7148963138937816 98 42-43 2.2954647696306765 98 44-45 1.564329745122528 98 46-47 1.671845419880917 98 48-49 1.561996637615529 98 50-51 0.3124017781344861 98 52-53 -0.017689280009534514 98 54-55 0.24859482613531014 98 56-57 0.2740477892678115 98 58-59 0.1781940985728383 98 60-61 0.2143618364914488 98 62-63 0.23862893267448726 98 64-65 0.36546016067674714 98 66-67 0.4499863632314103 98 68-69 0.4322930444812414 98 70-71 0.5553156875552219 98 72-73 0.5455582060240047 98 74-75 0.6054336405659768 98 76-77 0.40554887593215483 98 78-79 0.6496902414337384 98 80 0.23113167691224135 99 1 0.022310371532356044 99 2 0.08648889765042611 99 3 -0.19704728082410838 99 4 -0.16077908162947807 99 5 0.0697854182024713 99 6 0.0026526001471296468 99 7 -0.35016303594640164 99 8 -0.6441253343750901 99 9 -0.09602383403350956 99 10-11 0.03922272670031646 99 12-13 0.110623031149089 99 14-15 0.22993807523582177 99 16-17 0.9544228016136742 99 18-19 0.7679557662915073 99 20-21 0.2315735083922874 99 22-23 0.823315505917023 99 24-25 0.6778708119377868 99 26-27 0.5798986270621214 99 28-29 1.4012645257679495 99 30-31 0.7747170193242994 99 32-33 0.13183324098624638 99 34-35 -0.048427702378390336 99 36-37 0.31341515406454334 99 38-39 1.1683466731855248 99 40-41 1.6198001793585277 99 42-43 2.2054661596471306 99 44-45 1.3282125816708472 99 46-47 1.4014552775985365 99 48-49 1.2659595772935504 99 50-51 0.45435632059189146 99 52-53 0.1790393594623012 99 54-55 0.052847723648405065 99 56-57 -0.08197159777195395 99 58-59 -0.19289844781086884 99 60-61 -0.9717536848317181 99 62-63 -0.42548959087265104 99 64-65 -0.20830627968680915 99 66-67 -1.23944771848101 99 68-69 -2.0537475030970853 99 70-71 -9.601833398270315 99 72-73 -3.491718193118608 99 74-75 -3.5116247224444663 99 76-77 -0.41162429881051565 99 78-79 -0.43153226834007796 99 80 -0.6896671803275041 100 1 -0.34750616011518076 100 2 -0.885766005981651 100 3 -0.291476343168263 100 4 -0.21389890528066502 100 5 -0.12832486985521285 100 6 -0.07450963946050138 100 7 -0.13019154509285613 100 8 -0.5430502500610999 100 9 -0.17011836798273094 100 10-11 -0.07144446109461455 100 12-13 0.028096980676309613 100 14-15 0.22525909758534368 100 16-17 0.9257433255645466 100 18-19 0.6639129350028554 100 20-21 0.2111712312559817 100 22-23 0.7533722173600168 100 24-25 0.5713255917140785 100 26-27 0.4562069188596496 100 28-29 1.386322441364392 100 30-31 0.8747455036747951 100 32-33 0.17917240901385156 100 34-35 0.2567840349097281 100 36-37 0.027038409940495 100 38-39 0.9279509421806935 100 40-41 1.3677385167837102 100 42-43 2.0879913732565143 100 44-45 1.3269861986533442 100 46-47 1.3322095859727838 100 48-49 1.377830596187131 100 50-51 0.5144601970361435 100 52-53 0.3795897986141199 100 54-55 0.2780431390007507 100 56-57 0.08973269313663934 100 58-59 -0.17504887679634606 100 60-61 -0.00789661242164641 100 62-63 -0.23603041229437594 100 64-65 0.011977144249897265 100 66-67 0.2462041655619558 100 68-69 0.3183013089753146 100 70-71 0.5054202547159079 100 72-73 0.3182996940616718 100 74-75 0.47677752245710536 100 76-77 0.31658246100007403 100 78-79 -0.2990873347265932 100 80 -0.9733705178317251 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2851.0 5 236.0 6 304.0 7 493.0 8 833.0 9 1382.0 10 1911.0 11 3094.0 12 4176.0 13 5218.0 14 5743.0 15 5901.0 16 6725.0 17 6984.0 18 7877.0 19 8849.0 20 10235.0 21 12474.0 22 14689.0 23 18123.0 24 22498.0 25 29145.0 26 38250.0 27 52468.0 28 74020.0 29 102711.0 30 136510.0 31 176428.0 32 223303.0 33 273178.0 34 340082.0 35 443728.0 36 626183.0 37 1031815.0 38 1620577.0 39 2934756.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.675812521780664 13.84634817799617 12.787043625389138 46.69079567483403 2 26.029328171236187 21.77792485344527 32.40999951720579 19.782747458112752 3 23.736557691962442 27.029586542431915 23.621100499433027 25.61275526617262 4 26.282083941071033 31.68124509321556 18.203867007096825 23.83280395861658 5 26.454497274847554 33.486750401121625 21.098409111668598 18.960343212362226 6 19.105810832170132 35.873076833032115 22.886768038706613 22.13434429609114 7 18.363940527616105 15.348180362866017 42.13503815431771 24.15284095520017 8 20.565108708296787 20.576960097935906 26.93575831122671 31.9221728825406 9 22.33959047080343 19.59684896174185 30.269419569397893 27.794140998056832 10-11 24.784001272363028 29.296219348203895 20.479270084832088 25.440509294600993 12-13 23.13238182155961 23.184512612285143 27.375506728571803 26.307598837583445 14-15 23.488465638066323 24.79018370139156 26.408668666806008 25.312681993736106 16-17 24.58392911242234 24.74519052749914 24.84330719335206 25.82757316672646 18-19 24.171194752280943 24.93961474254264 24.899396317251384 25.989794187925035 20-21 24.172020499463784 25.560301509519096 25.296666607825006 24.971011383192117 22-23 24.01150979789681 25.346556103835642 25.01019862419559 25.63173547407196 24-25 23.888998938469697 25.45355219627357 24.994780900315014 25.662667964941722 26-27 24.052050877855905 25.71832431327571 25.29487188040756 24.934752928460824 28-29 24.297045182401114 24.4684660663438 24.810901451127336 26.42358730012775 30-31 23.870532777646744 25.08779764740785 25.22288542441913 25.818784150526277 32-33 23.954013989624297 25.934894860681773 25.454575449971923 24.656515699722007 34-35 24.262424072107333 25.659622856715142 25.187567469303573 24.890385601873955 36-37 24.129537815676507 25.68866327887736 25.243178932239847 24.938619973206283 38-39 24.285746779724448 24.952617451007804 25.15472759469762 25.606908174570126 40-41 24.2174988582437 24.634020777083375 24.88573793063957 26.26274243403336 42-43 24.05887690242433 24.66892540456517 25.042029061732023 26.230168631278477 44-45 24.130482513765795 25.186807849079283 25.115112959807046 25.567596677347876 46-47 24.33937622031033 24.970194458559558 24.97051599198035 25.719913329149758 48-49 23.996954604189543 25.087345834420255 25.09992780834633 25.815771753043883 50-51 24.310260929141304 25.767225030982555 25.300574623094835 24.621939416781306 52-53 24.384137139380226 25.565843203325084 25.275071723955055 24.774947933339632 54-55 24.17004477812182 25.564366396975746 25.41317321541947 24.852415609482957 56-57 24.28509182024223 25.648809347629154 25.438121594678904 24.627977237449713 58-59 24.458913042463 25.56809225312232 25.31762382269193 24.65537088172275 60-61 24.174385643994547 25.534556194247983 25.430346814882032 24.860711346875437 62-63 24.28701059110356 25.741996202339017 25.440859287665933 24.530133918891487 64-65 24.42248942483333 25.647730664426092 25.23841926527497 24.691360645465608 66-67 24.340465784184353 25.533496361821108 25.361651097403627 24.764386756590913 68-69 24.42723613207816 25.67493818047722 25.36661387923343 24.531211808211182 70-71 24.47227738117677 25.508637303641237 25.3195682798542 24.699517035327798 72-73 24.340256119561225 25.539110670037957 25.406396233740796 24.71423697666002 74-75 24.39410831879001 25.751947624863853 25.383705418850795 24.470238637495346 76-77 24.581111702069897 25.606654043592858 25.356210738559014 24.456023515778224 78-79 24.32610659280914 25.608741601693417 25.464549945701265 24.60060185979618 80 24.767972959908217 25.695583774343717 25.216773076456988 24.319670189291074 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11.0 1 8.5 2 7.5 3 9.5 4 16.0 5 22.0 6 26.0 7 38.0 8 54.0 9 76.0 10 90.0 11 106.0 12 143.0 13 208.5 14 337.0 15 421.0 16 497.0 17 694.0 18 973.5 19 1375.5 20 1619.0 21 2010.0 22 2969.5 23 4161.0 24 5978.0 25 7172.0 26 8925.0 27 12796.0 28 17777.0 29 25783.5 30 30927.0 31 36359.5 32 48219.5 33 63049.5 34 81671.0 35 91890.0 36 107072.0 37 135579.0 38 166669.0 39 203454.5 40 222475.0 41 245938.5 42 292385.0 43 344281.0 44 398340.0 45 423486.0 46 447167.0 47 490692.0 48 526468.0 49 547332.5 50 552265.0 51 550581.5 52 541359.5 53 513573.0 54 470492.0 55 447659.0 56 417575.5 57 355343.5 58 293391.0 59 236380.5 60 209174.0 61 184627.5 62 138401.0 63 101176.5 64 72241.5 65 58851.0 66 49652.0 67 33790.0 68 22731.0 69 15341.0 70 12347.0 71 10472.0 72 6807.0 73 4243.5 74 2628.5 75 1787.0 76 1321.5 77 684.5 78 406.5 79 251.5 80 203.0 81 165.5 82 111.5 83 89.0 84 64.0 85 45.0 86 39.0 87 35.5 88 32.0 89 20.5 90 15.0 91 16.0 92 12.0 93 8.0 94 7.0 95 5.0 96 5.5 97 8.0 98 9.5 99 7.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030362362606670086 2 9.82561474686487E-4 3 1.3343427434014021E-4 4 8.612575889227232E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.010050026935527834 12-13 8.551923946345351E-4 14-15 0.0 16-17 1.8195582864564574E-5 18-19 3.0325971440940956E-4 20-21 0.01576343995500111 22-23 0.0 24-25 0.0 26-27 0.00114025652617938 28-29 0.003711898904371174 30-31 0.07013790674860824 32-33 9.461703089573579E-4 34-35 0.00798786087754385 36-37 4.245636001731734E-4 38-39 0.005840782099525229 40-41 0.011833194056255162 42-43 0.0187475155447897 44-45 0.05674595776028873 46-47 0.024297168318481898 48-49 0.021943872934664876 50-51 9.097791432282288E-5 52-53 0.0 54-55 1.2130388576376383E-5 56-57 5.216067087841845E-4 58-59 6.671713717007011E-5 60-61 0.03806515935266909 62-63 6.065194288188192E-6 64-65 4.306287944613616E-4 66-67 6.671713717007011E-5 68-69 4.852155430550553E-5 70-71 0.05385892527911114 72-73 0.06999840727997993 74-75 0.0 76-77 0.02393932185547879 78-79 0.07834411462052687 80 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 80 8243759.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.07608824740172 #Duplication Level Percentage of deduplicated Percentage of total 1 76.07531449652666 43.42081363652595 2 12.805922582459786 14.618239348117386 3 4.4741332859272624 7.660980787746656 4 2.11291806270028 4.823883912248411 5 1.1951519575130287 3.410729929803427 6 0.7684189765818914 2.631500959101969 7 0.48837898686548176 1.9512333506757618 8 0.361648286615534 1.651317561711188 9 0.2617332921640306 1.3444841232753466 >10 1.3684718794291622 13.694576783406303 >50 0.06687998400265766 2.5919837484352954 >100 0.020344368519599382 1.9073285654230128 >500 5.769905861256914E-4 0.21789028862450313 >1k 1.068501085417947E-4 0.07503700490472141 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.011293391764606413 0.0 0.0 0.0 0.0 2 0.011984823913459867 0.0 0.0 0.0 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