##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename SRR950093_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8465707 Sequences flagged as poor quality 0 Sequence length 80 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.604028110115316 39.0 38.0 40.0 36.0 40.0 2 38.125683418998555 39.0 38.0 40.0 35.0 40.0 3 38.11627262790928 39.0 38.0 40.0 35.0 40.0 4 38.14694236405772 39.0 38.0 40.0 35.0 40.0 5 38.12963134679714 39.0 38.0 40.0 35.0 40.0 6 38.24029333876072 39.0 38.0 40.0 35.0 40.0 7 38.247370243264974 39.0 38.0 40.0 35.0 40.0 8 38.1902285302338 39.0 38.0 40.0 35.0 40.0 9 38.306620699251695 39.0 38.0 40.0 35.0 40.0 10-11 38.23157569710362 39.0 38.0 40.0 35.0 40.0 12-13 38.12967139070606 39.0 38.0 40.0 35.0 40.0 14-15 37.96911651915191 39.0 38.0 40.0 34.5 40.0 16-17 37.586324154615795 39.0 38.0 40.0 33.0 40.0 18-19 37.43204442346044 39.0 37.5 40.0 33.0 40.0 20-21 37.69371766587244 39.0 38.0 40.0 33.0 40.0 22-23 37.49211701987796 39.0 38.0 40.0 33.0 40.0 24-25 37.34851991688349 39.0 37.5 40.0 32.5 40.0 26-27 37.503311891139155 39.0 38.0 40.0 33.0 40.0 28-29 36.819058703543604 39.0 37.0 40.0 31.5 40.0 30-31 37.048965372886165 39.0 37.0 40.0 32.0 40.0 32-33 37.41658706118697 39.0 37.0 40.0 33.0 40.0 34-35 37.422047680128784 39.0 37.0 40.0 33.0 40.0 36-37 37.61172386429155 39.0 38.0 40.0 33.0 40.0 38-39 37.05385220631898 39.0 38.0 40.0 32.5 40.0 40-41 36.65466575916223 39.0 37.5 40.0 31.0 40.0 42-43 36.19097442186459 39.0 37.0 40.0 29.5 40.0 44-45 36.60525506020939 39.0 37.0 40.0 30.0 40.0 46-47 36.52699083490605 39.0 37.0 40.0 30.5 40.0 48-49 36.46380574002856 39.0 36.0 40.0 30.0 40.0 50-51 37.04086085190522 39.0 36.5 40.0 32.0 40.0 52-53 37.14996680135516 39.0 36.5 40.0 33.0 40.0 54-55 37.09047460536964 39.0 36.0 40.0 33.0 40.0 56-57 37.11559855544256 39.0 36.5 40.0 33.0 40.0 58-59 36.98260458340928 39.0 36.0 40.0 33.0 40.0 60-61 36.84264657399554 39.0 36.0 40.0 33.0 40.0 62-63 36.6983667164479 39.0 36.0 40.0 32.0 40.0 64-65 36.527950943730986 39.0 36.0 40.0 31.5 40.0 66-67 36.33412188728006 38.5 35.5 40.0 31.0 40.0 68-69 36.05845205840457 38.0 35.0 40.0 30.5 40.0 70-71 35.73039327961622 38.0 35.0 40.0 30.0 40.0 72-73 35.567845898753646 38.0 35.0 39.5 29.0 40.0 74-75 35.30722726406667 38.0 35.0 39.0 29.0 40.0 76-77 35.10449765152515 38.0 35.0 39.0 29.0 40.0 78-79 34.80599316749328 38.0 34.0 39.0 28.5 40.0 80 33.930044826734495 37.0 33.0 39.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.05432906738280252 1 2 0.030019564907973972 1 3 -0.21643437886658745 1 4 -0.16231225889536205 1 5 -0.3551840213927804 1 6 -0.4829391479851992 1 7 -0.4462148069571015 1 8 -0.05015563906606957 1 9 -0.13895294963673877 1 10-11 -0.4717933639151397 1 12-13 -0.4205342285999194 1 14-15 -0.251904171879616 1 16-17 0.45447593793523566 1 18-19 0.3593051982601594 1 20-21 -0.338813492018339 1 22-23 0.5920025255492192 1 24-25 -0.20143055642615337 1 26-27 0.5665653328021065 1 28-29 1.1580405826010605 1 30-31 0.6661996353398081 1 32-33 0.43042547596608216 1 34-35 0.40588328100812987 1 36-37 0.42366841047270043 1 38-39 1.2478725214513418 1 40-41 1.568502227024986 1 42-43 2.247012204046328 1 44-45 1.4173280998205087 1 46-47 1.3443858756609472 1 48-49 1.3385430447265279 1 50-51 0.5225474674238484 1 52-53 0.20988463306917993 1 54-55 0.13505627804048714 1 56-57 -0.4438713729905004 1 58-59 -0.25681151660992896 1 60-61 -0.027222664355143422 1 62-63 -0.13415811719168858 1 64-65 -0.11488952217645476 1 66-67 -0.01038381689800616 1 68-69 -0.05263403544329748 1 70-71 -0.1643442782789677 1 72-73 0.07482916221867697 1 74-75 -0.08230484639998537 1 76-77 -0.13523342905448743 1 78-79 -0.13606053568974374 1 80 -0.4572882905799176 2 1 -0.039136807212784674 2 2 0.06784902999809361 2 3 0.08792476338659583 2 4 -0.04825609301989431 2 5 0.02321453418719699 2 6 -0.7759848427391347 2 7 -0.11807792470531808 2 8 -0.02154595562254258 2 9 -0.08146877348700343 2 10-11 -0.4561850286012614 2 12-13 -1.3510030835617712 2 14-15 -1.3662455117978354 2 16-17 -0.7863250613539137 2 18-19 0.06823822984213024 2 20-21 -0.24821399531434452 2 22-23 0.08525614511032131 2 24-25 0.5263813109165127 2 26-27 0.2563860384324741 2 28-29 1.0750159383443716 2 30-31 0.8502910181891892 2 32-33 0.3185372798705117 2 34-35 0.2511946401038756 2 36-37 0.2229411636373939 2 38-39 1.0069373320886967 2 40-41 1.561328145931654 2 42-43 1.9935752547478387 2 44-45 1.4313362493839605 2 46-47 1.309848591386654 2 48-49 -0.010761180547838478 2 50-51 0.4510395291600915 2 52-53 0.10015294233546967 2 54-55 -0.02578620472180404 2 56-57 0.036045611198488814 2 58-59 -0.04941251544801872 2 60-61 -0.012673445272632478 2 62-63 0.009064682684453373 2 64-65 -0.021048830440861366 2 66-67 -0.05709021846794826 2 68-69 -0.00922617784393509 2 70-71 -0.07378869271246913 2 72-73 -0.19576799381092513 2 74-75 -0.15375160977729507 2 76-77 -0.5001913237641915 2 78-79 -0.35734320216624127 2 80 -0.4157545834188454 3 1 0.020617387160257294 3 2 -0.24168840627963561 3 3 0.14930909296306538 3 4 0.03305454446526568 3 5 -0.04727618979243431 3 6 -0.25059950598347314 3 7 -0.30966569470228933 3 8 -0.054392998402008175 3 9 -0.008983808754152278 3 10-11 -1.0410875873561238 3 12-13 -0.5763369622755974 3 14-15 -0.8671566455490662 3 16-17 -0.6546201584496956 3 18-19 -0.06058787122091758 3 20-21 -0.8675431549330526 3 22-23 -0.7754669856329457 3 24-25 0.27814504123738004 3 26-27 0.4656035727063781 3 28-29 1.330241757379575 3 30-31 0.9025685460392623 3 32-33 -0.4153122502869664 3 34-35 0.1751674061133599 3 36-37 0.3195148793258298 3 38-39 1.1699902799394124 3 40-41 1.7011638005333651 3 42-43 2.304178352166275 3 44-45 1.651702807433864 3 46-47 1.5487280250068736 3 48-49 1.5467042921803156 3 50-51 0.69096574207272 3 52-53 0.31959692706463017 3 54-55 0.2596991742104464 3 56-57 0.12763859907514075 3 58-59 0.09496369552740447 3 60-61 0.04865918877185038 3 62-63 0.07689000767938126 3 64-65 0.0790477065466959 3 66-67 0.044326515259776045 3 68-69 0.07266399775987509 3 70-71 -1.0756134317694688 3 72-73 0.015776156976329503 3 74-75 -0.25124042889255094 3 76-77 0.004497456844525516 3 78-79 -0.06062874318276812 3 80 -0.15197502476558356 4 1 0.04129995437440925 4 2 0.22704806716863857 4 3 0.20150204959761453 4 4 0.23138298915943523 4 5 0.06414369046398605 4 6 -0.1040779153230531 4 7 0.09181099174061558 4 8 -0.10744932448766065 4 9 0.05945013181992209 4 10-11 -0.19335907250185613 4 12-13 -0.6111022669253074 4 14-15 -0.6154629232015836 4 16-17 -0.6201511001423299 4 18-19 -0.1451513780614846 4 20-21 -0.3154097800146829 4 22-23 -0.07714372521571278 4 24-25 0.5010512367943889 4 26-27 0.6016564187592834 4 28-29 1.3139968699043365 4 30-31 0.8034899698545246 4 32-33 0.36036747279607084 4 34-35 0.393421261529312 4 36-37 0.35612387226279907 4 38-39 1.2506856485707374 4 40-41 1.8162371790342675 4 42-43 2.312673188442517 4 44-45 1.6582908380434134 4 46-47 1.6254739419868542 4 48-49 1.6021855382526837 4 50-51 0.6887167945660906 4 52-53 0.3552385243892715 4 54-55 0.3690754751761247 4 56-57 0.1988485871017076 4 58-59 0.09630808549054137 4 60-61 0.06242903022930335 4 62-63 0.04916322073576396 4 64-65 -0.1184628690716778 4 66-67 0.012082022688822747 4 68-69 0.062124669324155946 4 70-71 0.027995391769039202 4 72-73 -0.5276295047636523 4 74-75 -0.20347549875065596 4 76-77 -0.12908178743068532 4 78-79 -0.17998118707339472 4 80 -0.15061613108946403 5 1 0.07468660920014969 5 2 0.0253562553046649 5 3 -0.20014889995727714 5 4 0.29587916545273174 5 5 0.16521495158467303 5 6 0.05446746359190513 5 7 -1.0915349994741987 5 8 0.06565912117691397 5 9 -0.1088985420823434 5 10-11 -0.16054228792936698 5 12-13 0.07974583229335508 5 14-15 -0.2735311445552142 5 16-17 0.42839558152432033 5 18-19 0.2245243096895564 5 20-21 -1.084661339660805 5 22-23 0.4445749278185218 5 24-25 0.7239315317248796 5 26-27 0.7578721961983561 5 28-29 1.5720987348564037 5 30-31 1.0350090246210257 5 32-33 0.33129529601131935 5 34-35 0.45881765851920875 5 36-37 0.42721131895513764 5 38-39 1.346518852444234 5 40-41 1.9334639644431277 5 42-43 2.350665804294323 5 44-45 1.7149560182554922 5 46-47 1.7504573329848512 5 48-49 1.6163898007203343 5 50-51 0.7835511106640141 5 52-53 0.4653773530220491 5 54-55 0.36389896189653115 5 56-57 0.19797006581786292 5 58-59 0.2464147339401066 5 60-61 0.19963946651760267 5 62-63 0.20078124082520787 5 64-65 0.14734473342939225 5 66-67 0.17794783522533208 5 68-69 0.11718032231758713 5 70-71 0.24835111183588765 5 72-73 0.3025294407789261 5 74-75 0.27081878277011384 5 76-77 0.13949048682108156 5 78-79 0.20727680889789468 5 80 0.05364286098258475 6 1 -0.14144062610646557 6 2 0.03916342514882132 6 3 -0.02375909471442128 6 4 0.03752430169510035 6 5 -0.15269996051076618 6 6 -0.21505221273422137 6 7 0.09418474282941247 6 8 -0.22418924482638403 6 9 -0.05758279796865651 6 10-11 -0.16902786192206776 6 12-13 -0.17131589876106545 6 14-15 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0.15194456868653106 7 5 0.1289399484923095 7 6 -0.05111269667614238 7 7 -0.024191011539450358 7 8 0.08099124919084488 7 9 0.03538391833790655 7 10-11 -0.037133407778817684 7 12-13 -0.2829750616392701 7 14-15 -0.21818187604993255 7 16-17 0.4872243894965891 7 18-19 0.4415396285234223 7 20-21 -0.012223136273028956 7 22-23 0.6731245952200879 7 24-25 0.8743886328399171 7 26-27 0.3304911001602946 7 28-29 1.195618969542501 7 30-31 0.9342710224206456 7 32-33 0.330640155571146 7 34-35 0.3546157867896369 7 36-37 0.40914735148979275 7 38-39 1.1538060702499138 7 40-41 1.7474640888292612 7 42-43 2.2856542613071227 7 44-45 1.6167741690563133 7 46-47 1.2476309167817163 7 48-49 1.5609716472453172 7 50-51 0.6663312796104108 7 52-53 0.31322445981873415 7 54-55 0.3130087480550898 7 56-57 0.06928903605333403 7 58-59 0.008369551701228772 7 60-61 -0.06422314535915774 7 62-63 0.041489067605013474 7 64-65 0.13737892194215817 7 66-67 0.049096306035359305 7 68-69 0.1205135428598183 7 70-71 0.27938756570808465 7 72-73 0.24120555862310766 7 74-75 0.13332269257008278 7 76-77 -0.1564606676454119 7 78-79 0.05698117153921345 7 80 -0.003939031060134823 8 1 -0.6783002456056408 8 2 -0.30369143684757205 8 3 -0.2643021756478845 8 4 -0.2881776442962476 8 5 -0.25473218081955196 8 6 -0.2541563944661078 8 7 -0.18932371978523577 8 8 -0.17033714613273787 8 9 -0.3615488953253774 8 10-11 -0.20550968612432285 8 12-13 -0.15416430290402872 8 14-15 -0.33786644295086177 8 16-17 0.1607555813978152 8 18-19 0.4615424018883729 8 20-21 0.2703235963822621 8 22-23 0.7016294255952857 8 24-25 0.791931496883528 8 26-27 0.6032012120562982 8 28-29 1.4184245528283057 8 30-31 0.970941264475286 8 32-33 0.4257567778915643 8 34-35 0.3765742319847476 8 36-37 0.4551480841403759 8 38-39 1.2475744765504828 8 40-41 1.8231155997129207 8 42-43 2.4001510674675615 8 44-45 1.6771201433962801 8 46-47 1.0262215431862174 8 48-49 1.628018689931018 8 50-51 0.764938145193085 8 52-53 0.4244313241345239 8 54-55 0.43995919651877813 8 56-57 0.08880050646394011 8 58-59 0.265381582761691 8 60-61 0.27461054612598446 8 62-63 0.2914531170509136 8 64-65 0.372509198065913 8 66-67 0.3856307967543202 8 68-69 0.43164431204893106 8 70-71 0.667952980493439 8 72-73 0.16122073524850578 8 74-75 0.5702811035387398 8 76-77 0.4982847952797229 8 78-79 -0.09413300445773842 8 80 0.08227415237968927 9 1 0.12241551970997477 9 2 0.15922329952728376 9 3 -0.21773301771203535 9 4 0.12789098681906808 9 5 0.2184354247528475 9 6 -0.05492145950838534 9 7 -0.11971328149321181 9 8 -0.18227951036920587 9 9 0.09617424237453776 9 10-11 0.03593112883623206 9 12-13 -0.1278552962512478 9 14-15 -0.2457233531899874 9 16-17 0.6365541089689728 9 18-19 0.40303605793283026 9 20-21 0.03235148846868441 9 22-23 0.4955706288251278 9 24-25 -1.0568586001628475 9 26-27 0.3240125738474049 9 28-29 1.236996729567558 9 30-31 0.6939132420607024 9 32-33 0.5095913509714478 9 34-35 0.4421972106377794 9 36-37 0.405769714517632 9 38-39 1.2834190045290725 9 40-41 1.8498122744366583 9 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0.7763528074914277 10 26-27 0.6170887693010982 10 28-29 1.4720278966742413 10 30-31 1.1381781883918762 10 32-33 0.4825242369569054 10 34-35 0.4338139307039697 10 36-37 0.48253356386575774 10 38-39 1.397513859252527 10 40-41 1.9359267392129595 10 42-43 2.485237969873367 10 44-45 1.8048516332255957 10 46-47 1.6551783560612137 10 48-49 1.8094239117353297 10 50-51 0.6890614173591061 10 52-53 0.589856043682552 10 54-55 0.48435834530437916 10 56-57 0.4019146242040321 10 58-59 0.29260132434576036 10 60-61 -0.2292463805556153 10 62-63 0.3248712022278113 10 64-65 -0.5087496751941103 10 66-67 0.4168471587803353 10 68-69 0.5513666199259362 10 70-71 0.6946409321591958 10 72-73 0.555220349570007 10 74-75 0.7731520371229621 10 76-77 0.781883103449104 10 78-79 0.7380079962382595 10 80 0.5115566347099758 11 1 -0.05836944581201209 11 2 0.23957483947084768 11 3 0.2391173890787428 11 4 0.09154755591136166 11 5 0.07461538812608381 11 6 -0.034947040509308636 11 7 0.005270184063498107 11 8 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2.2543129397175505 96 44-45 1.4679035691591409 96 46-47 1.6068993764886272 96 48-49 1.3704179181822767 96 50-51 0.4944895693288913 96 52-53 -0.810378465774825 96 54-55 0.12724926933423575 96 56-57 -0.9987305172361118 96 58-59 -0.13814973488636184 96 60-61 -0.029752608188324814 96 62-63 -0.025607902871257693 96 64-65 0.06059882914525616 96 66-67 0.18823143879782123 96 68-69 0.31424517861146484 96 70-71 0.4561531985701919 96 72-73 0.24865551664358065 96 74-75 0.4453219177094425 96 76-77 0.5899250544131078 96 78-79 0.6647056559687243 96 80 0.2208247414968767 97 1 -0.022779182129482933 97 2 -0.03078817631482167 97 3 0.10041921822785582 97 4 0.02491529416732874 97 5 0.09057340860766772 97 6 -0.011750755539317481 97 7 0.1659993454052966 97 8 -0.10952420622039227 97 9 0.06616754456886298 97 10-11 0.07058408336864375 97 12-13 -0.020711622280906283 97 14-15 0.3188348130504579 97 16-17 0.824272007810464 97 18-19 0.6816158866179549 97 20-21 0.3058049294097387 97 22-23 0.7675033194005962 97 24-25 0.8653224589861779 97 26-27 0.7151477374278485 97 28-29 1.5602550610641472 97 30-31 1.0385631622003189 97 32-33 0.2424637887211034 97 34-35 0.39570860822978915 97 36-37 0.44878366668594083 97 38-39 1.282856629137953 97 40-41 1.8645240788285236 97 42-43 2.4307090292522773 97 44-45 1.7372065865502435 97 46-47 1.7494189086419567 97 48-49 1.6365154372457695 97 50-51 0.7805064898147336 97 52-53 0.430519469590827 97 54-55 0.40830580519448034 97 56-57 0.1471328337084259 97 58-59 0.22256480562646885 97 60-61 0.22032916017823112 97 62-63 0.2660816941998192 97 64-65 0.3013781127375097 97 66-67 0.4244639399368637 97 68-69 0.42760418620733986 97 70-71 0.47924735907260185 97 72-73 0.5042643099626716 97 74-75 0.4918496866034445 97 76-77 0.6206667005705455 97 78-79 0.7002768389143554 97 80 0.1726746562289847 98 1 -0.4131144630173722 98 2 -1.1900986686631327 98 3 -0.4338263692165327 98 4 -0.18308866613189423 98 5 -0.2750614615825242 98 6 -0.19804909808075877 98 7 -0.1156399006710771 98 8 -0.44439464087545133 98 9 -0.20217230777553397 98 10-11 -0.07546282906474033 98 12-13 0.04080386444476147 98 14-15 0.12570984360831972 98 16-17 0.8207224674007492 98 18-19 0.6780748412945314 98 20-21 0.1632385577283273 98 22-23 0.7748062211753961 98 24-25 0.7439011515985072 98 26-27 0.620974572122023 98 28-29 1.4307778450078175 98 30-31 0.9460049800265509 98 32-33 0.059606506170865714 98 34-35 -0.04599682618081147 98 36-37 0.3523391353213512 98 38-39 1.2019406877112644 98 40-41 1.714296422738954 98 42-43 2.280345329550528 98 44-45 1.5471464486731605 98 46-47 1.6097569397376361 98 48-49 1.5639755286749022 98 50-51 0.2902902012507127 98 52-53 -0.04328339551453553 98 54-55 0.1598490384609974 98 56-57 0.17173020328746702 98 58-59 0.13291524321508774 98 60-61 0.16365196123430792 98 62-63 0.21747030467260942 98 64-65 0.33335361755342774 98 66-67 0.4516072074281823 98 68-69 0.48272085613351123 98 70-71 0.5863211990960764 98 72-73 0.6417120560145975 98 74-75 0.6548019429961514 98 76-77 0.3401109141333407 98 78-79 0.7165961441507491 98 80 0.2638510117758486 99 1 0.04976336512451951 99 2 0.1130782872244751 99 3 -0.14997822699897512 99 4 -0.10399670830546626 99 5 0.07375978224639113 99 6 0.005154970947046422 99 7 -0.36161120381376577 99 8 -0.6626141145466136 99 9 -0.07128166955205728 99 10-11 0.05116676321582503 99 12-13 0.13002755874017424 99 14-15 0.24961187939258167 99 16-17 0.9901214473610978 99 18-19 0.7348150457501106 99 20-21 0.20560251261719031 99 22-23 0.8332537549503769 99 24-25 0.6698144839195876 99 26-27 0.6312394424574563 99 28-29 1.4518514818042618 99 30-31 0.7904892103055516 99 32-33 0.17815488848670924 99 34-35 -0.06147828404746747 99 36-37 0.25348048237619736 99 38-39 1.0949785813795998 99 40-41 1.5850528563628785 99 42-43 2.2099260679692208 99 44-45 1.2802415690943434 99 46-47 1.3527968115400313 99 48-49 1.2371043560164665 99 50-51 0.39163602808352493 99 52-53 0.13645200637031962 99 54-55 0.01237743859819318 99 56-57 -0.168071632445141 99 58-59 -0.23604741602621715 99 60-61 -1.058876626432422 99 62-63 -0.5313810890649933 99 64-65 -0.38317588235371147 99 66-67 -1.505831350941591 99 68-69 -2.2492988550347164 99 70-71 -9.88508311880156 99 72-73 -3.5880996945856474 99 74-75 -3.669748182739376 99 76-77 -0.5087406973804818 99 78-79 -0.5431520097420375 99 80 -0.7075336034226325 100 1 -0.38877693140301517 100 2 -0.896649950263857 100 3 -0.3536272851390123 100 4 -0.22483240900464807 100 5 -0.1369507728713515 100 6 -0.06040177549984094 100 7 -0.07231107141100068 100 8 -0.5183313988394858 100 9 -0.18587349260937458 100 10-11 -0.03423622247392899 100 12-13 0.09311577495144974 100 14-15 0.26550167059374274 100 16-17 0.9292443638619972 100 18-19 0.6534778750605383 100 20-21 0.21400188301895895 100 22-23 0.7607158480563498 100 24-25 0.6012261562338708 100 26-27 0.43189661449100925 100 28-29 1.3653223256598963 100 30-31 0.8908682642351877 100 32-33 0.12573911497453594 100 34-35 0.22888621131771458 100 36-37 -0.025522043889466772 100 38-39 0.9114532177199024 100 40-41 1.3897960416095287 100 42-43 2.0532717006772714 100 44-45 1.2445387720357317 100 46-47 1.3093620557185304 100 48-49 1.3175757414772207 100 50-51 0.48067452998423477 100 52-53 0.3538201662581244 100 54-55 0.2628832164999366 100 56-57 0.11360885022345713 100 58-59 -0.16395226068406288 100 60-61 -0.007390018748218097 100 62-63 -0.18654804770911682 100 64-65 0.10118628173901811 100 66-67 0.3138742549415028 100 68-69 0.4006120530738073 100 70-71 0.4573173708357814 100 72-73 0.19787435534601627 100 74-75 0.456841985217288 100 76-77 0.23644505496823598 100 78-79 -0.39355078154658685 100 80 -1.1894289917143013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3042.0 5 272.0 6 274.0 7 566.0 8 799.0 9 1477.0 10 1980.0 11 3140.0 12 4368.0 13 5419.0 14 6002.0 15 5918.0 16 6439.0 17 6901.0 18 7838.0 19 8714.0 20 10230.0 21 12443.0 22 14562.0 23 17866.0 24 22577.0 25 29072.0 26 38281.0 27 52594.0 28 74651.0 29 104344.0 30 139791.0 31 179671.0 32 226367.0 33 277204.0 34 345011.0 35 451872.0 36 637524.0 37 1056021.0 38 1671309.0 39 3041164.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.47934353814563 13.727625425584533 9.9669197900619 51.82611124620794 2 24.797933007490403 21.27700314908525 35.43563724059382 18.489426602830527 3 22.778737073428758 27.009538015866845 23.864163893319184 26.347561017385207 4 26.26531473591837 31.56157120664863 18.230641319756696 23.942472737676304 5 26.676614250883002 33.36263586727015 21.16914747935406 18.791602402492785 6 19.0901362402455 35.60891015954131 23.393651587516555 21.907302012696636 7 18.40303473767755 15.385436798131568 42.04831327141372 24.163215192777166 8 20.05683636346025 20.42470876915537 27.811427917361186 31.70702695002319 9 22.02223629993337 19.347385871020577 31.09736729608053 27.53301053296553 10-11 24.6818633819568 29.171166387227693 20.644173527614544 25.502796703200964 12-13 23.088962716869695 23.105972683647103 27.328360280546327 26.47670431893688 14-15 23.559001037952292 24.723694075403273 26.228411873928543 25.48889301271589 16-17 24.713770096011512 24.718878944909896 24.680293846813793 25.8870571122648 18-19 24.265030680064374 24.807609882092706 24.78445166195519 26.142907775887725 20-21 24.239705891387366 25.529957624655676 25.31326068988592 24.917075794071046 22-23 24.013971898625833 25.293091291725545 25.077568831522278 25.61536797812634 24-25 23.871691992175016 25.40024123206721 24.951253333005738 25.776813442752033 26-27 23.94052459144588 25.701595388829627 25.48060144231669 24.8772785774078 28-29 24.26975637519057 24.411504059940977 24.781350992050694 26.537388572817754 30-31 23.860479463466753 24.9113570802363 25.12745365688282 26.100709799414123 32-33 24.046933447614812 25.87114723777167 25.318507847067096 24.763411467546426 34-35 24.25598275816445 25.737224559692436 25.104843572208928 24.901949109934186 36-37 24.154740508119293 25.588578670594213 25.226144252383108 25.030536568903383 38-39 24.2094856465597 25.009702959782228 25.117538562731028 25.663272830927042 40-41 24.171473787786194 24.64897452348867 24.810651861336122 26.36889982738902 42-43 24.07463614277851 24.598513274235913 24.976060664668186 26.350789918317393 44-45 24.048942494637128 25.210478728747955 25.095863939628526 25.64471483698639 46-47 24.356353112185054 24.949544388620602 24.878481478538607 25.815621020655744 48-49 24.035636846890956 24.98354171825879 25.037447612332205 25.94337382251805 50-51 24.33367573471201 25.737741884957387 25.304274969411857 24.624307410918746 52-53 24.514703851668855 25.458842362486678 25.18300597929978 24.843447806544685 54-55 24.096921154597148 25.464273150992963 25.41072179922147 25.028083895188423 56-57 24.33362084728969 25.546081612383936 25.410162165431327 24.71013537489505 58-59 24.48192719280693 25.425209864462513 25.297972926521627 24.794890016208935 60-61 24.18735068930264 25.48695383518409 25.40408165291067 24.9216138226026 62-63 24.256055888542793 25.582619714019145 25.573400164534405 24.587924232903656 64-65 24.388625459177636 25.67602382230933 25.1429714194185 24.792379299094534 66-67 24.304452247433233 25.446300850183484 25.389483352945803 24.85976354943748 68-69 24.412861987698488 25.686276235335615 25.31321813639749 24.58764364056841 70-71 24.423424347625065 25.485205223585126 25.305927247344982 24.78544318144482 72-73 24.32882879475125 25.451578791086842 25.311415306620848 24.90817710754106 74-75 24.402905746678925 25.639447479105996 25.403601849201728 24.55404492501335 76-77 24.54205057792421 25.618477033498422 25.310341763775053 24.529130624802317 78-79 24.254968296623872 25.521306446739324 25.5162408577053 24.70748439893151 80 24.747998011270646 25.688403815534837 25.201817166599316 24.361781006595194 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4.0 1 4.0 2 3.5 3 5.0 4 10.0 5 13.0 6 12.0 7 12.5 8 17.0 9 24.5 10 29.0 11 32.0 12 57.0 13 105.0 14 178.0 15 225.0 16 270.5 17 390.5 18 624.5 19 920.0 20 1056.0 21 1369.5 22 2167.0 23 3364.5 24 5329.0 25 6580.0 26 8220.5 27 12295.5 28 17430.0 29 24402.0 30 28674.0 31 33237.5 32 44260.0 33 59718.5 34 81676.5 35 94635.0 36 107636.0 37 137983.0 38 170893.0 39 203912.0 40 221367.0 41 243555.5 42 291992.0 43 349056.0 44 407387.0 45 434902.0 46 464990.0 47 518277.0 48 553384.0 49 570038.0 50 574784.0 51 572833.0 52 563107.5 53 537847.0 54 495032.5 55 469704.0 56 436865.0 57 367061.0 58 299077.0 59 238597.0 60 209136.0 61 185650.0 62 139009.5 63 99344.5 64 70511.5 65 58189.0 66 48923.5 67 33322.5 68 22344.5 69 14779.0 70 11856.0 71 9823.0 72 6353.0 73 3934.0 74 2295.0 75 1638.0 76 1311.0 77 840.0 78 568.0 79 336.5 80 233.0 81 196.0 82 130.5 83 96.5 84 73.0 85 55.0 86 45.5 87 31.0 88 24.0 89 22.0 90 22.0 91 19.0 92 16.0 93 12.0 94 7.5 95 7.0 96 6.5 97 5.5 98 6.5 99 6.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03119644939282685 2 8.741148258497489E-4 3 5.906181255741546E-5 4 0.001015863175987546 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.009798354703275225 12-13 9.686137259416136E-4 14-15 0.0 16-17 4.134326879019083E-5 18-19 3.6027705660023437E-4 20-21 0.01595850175301366 22-23 0.0 24-25 0.0 26-27 0.00106901880728922 28-29 0.0036972694660942084 30-31 0.07162426008837774 32-33 7.087417506889855E-4 34-35 0.008445839195710412 36-37 4.42963594180616E-4 38-39 0.005982961612066187 40-41 0.011906861411574958 42-43 0.01894702946841888 44-45 0.05619140846712507 46-47 0.02456380784262909 48-49 0.022242678609122664 50-51 1.0631126260334785E-4 52-53 0.0 54-55 1.1812362511483093E-5 56-57 3.6618323785597587E-4 58-59 5.906181255741546E-5 60-61 0.03871501813138584 62-63 5.906181255741547E-6 64-65 3.425585128330097E-4 66-67 5.906181255741546E-5 68-69 1.771854376722464E-5 70-71 0.053687187614690664 72-73 0.07020087040574402 74-75 0.0 76-77 0.024262592598586274 78-79 0.07880027031410372 80 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 80 8465707.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.45503654269618 #Duplication Level Percentage of deduplicated Percentage of total 1 75.56834107675886 41.15076774805832 2 12.693752359880852 13.824774972424954 3 4.6917032390227975 7.664606139854173 4 2.210221454563545 4.814307603028357 5 1.2440025191111377 3.387110131870155 6 0.792642844510516 2.5898037037876076 7 0.5276665398713078 2.011387049773508 8 0.37813052447404955 1.647288922251459 9 0.29451178589996657 1.4433885057093654 >10 1.4775713465880782 14.291267824922885 >50 0.08580157972906358 3.1789453577190643 >100 0.03390874836459897 3.1261079447107667 >500 0.0012440116229147182 0.45274703420882106 >1k 5.019696022287459E-4 0.4174970616805795 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.013584216888205557 0.0 0.0 0.0 0.0 2 0.014411082264009374 0.0 0.0 0.0 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