##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename SRR949916_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7930327 Sequences flagged as poor quality 0 Sequence length 80 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.6001539406887 39.0 38.0 40.0 36.0 40.0 2 38.12017373306296 39.0 38.0 40.0 35.0 40.0 3 38.114513815130195 39.0 38.0 40.0 35.0 40.0 4 38.14416013362374 39.0 38.0 40.0 35.0 40.0 5 38.12708971521603 39.0 38.0 40.0 35.0 40.0 6 38.23729866372471 39.0 38.0 40.0 35.0 40.0 7 38.243369409609464 39.0 38.0 40.0 35.0 40.0 8 38.18439542278647 39.0 38.0 40.0 35.0 40.0 9 38.30171580566602 39.0 38.0 40.0 35.0 40.0 10-11 38.22740310708499 39.0 38.0 40.0 35.0 40.0 12-13 38.1276529328488 39.0 38.0 40.0 35.0 40.0 14-15 37.96860066426012 39.0 38.0 40.0 34.5 40.0 16-17 37.58971710750389 39.0 38.0 40.0 33.0 40.0 18-19 37.43476120215472 39.0 37.5 40.0 33.0 40.0 20-21 37.69696281124347 39.0 38.0 40.0 33.0 40.0 22-23 37.498326739868354 39.0 38.0 40.0 33.0 40.0 24-25 37.3533214330254 39.0 37.5 40.0 32.5 40.0 26-27 37.50468372867853 39.0 38.0 40.0 33.0 40.0 28-29 36.82711885651121 39.0 37.0 40.0 31.5 40.0 30-31 37.05195914367718 39.0 37.0 40.0 32.0 40.0 32-33 37.41726885915297 39.0 37.0 40.0 33.0 40.0 34-35 37.42366531670132 39.0 37.0 40.0 33.0 40.0 36-37 37.61166620241511 39.0 38.0 40.0 33.0 40.0 38-39 37.059947275818516 39.0 38.0 40.0 32.5 40.0 40-41 36.66278017287307 39.0 37.5 40.0 31.0 40.0 42-43 36.20388894430205 39.0 37.0 40.0 29.5 40.0 44-45 36.614757184665905 39.0 37.0 40.0 30.0 40.0 46-47 36.53777788734311 39.0 37.0 40.0 30.5 40.0 48-49 36.47448686542182 39.0 36.0 40.0 30.0 40.0 50-51 37.04696029558428 39.0 36.5 40.0 32.0 40.0 52-53 37.15341385039986 39.0 36.5 40.0 33.0 40.0 54-55 37.092819060298524 39.0 36.0 40.0 33.0 40.0 56-57 37.12135489494948 39.0 36.5 40.0 33.0 40.0 58-59 36.98798687620321 39.0 36.0 40.0 33.0 40.0 60-61 36.85041707611805 39.0 36.0 40.0 33.0 40.0 62-63 36.708238891031854 39.0 36.0 40.0 32.0 40.0 64-65 36.540603243725 39.0 36.0 40.0 31.5 40.0 66-67 36.34625533095924 39.0 35.5 40.0 31.0 40.0 68-69 36.07505125576789 38.0 35.0 40.0 30.5 40.0 70-71 35.7487853275155 38.0 35.0 40.0 30.0 40.0 72-73 35.58563726312925 38.0 35.0 39.5 29.0 40.0 74-75 35.329623356010416 38.0 35.0 39.0 29.0 40.0 76-77 35.129494218838644 38.0 35.0 39.0 29.0 40.0 78-79 34.83570778355041 38.0 34.0 39.0 28.5 40.0 80 33.953531928759055 37.0 33.0 39.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.035510360680333974 1 2 0.0319170969092184 1 3 -0.18822826888725785 1 4 -0.1724412969541902 1 5 -0.33136401592531683 1 6 -0.4778871294639515 1 7 -0.452461560737639 1 8 -0.06397360355848747 1 9 -0.1346962332379107 1 10-11 -0.4570396664605241 1 12-13 -0.38164982340945386 1 14-15 -0.19405012156654777 1 16-17 0.46428597940118976 1 18-19 0.3767923921035887 1 20-21 -0.304055263784214 1 22-23 0.5839916897802055 1 24-25 -0.16609186277993615 1 26-27 0.5249253690224478 1 28-29 1.092369194298655 1 30-31 0.6415324780958045 1 32-33 0.35963676979292103 1 34-35 0.3079765504309435 1 36-37 0.35403853362562643 1 38-39 1.1675892003005046 1 40-41 1.5025244000136482 1 42-43 2.19947236836925 1 44-45 1.3367762654576225 1 46-47 1.3341977251064705 1 48-49 1.3053979633671986 1 50-51 0.4974192749132982 1 52-53 0.2003869452168061 1 54-55 0.12756128733659722 1 56-57 -0.46406545030777835 1 58-59 -0.30968859049565367 1 60-61 -0.060724940101913205 1 62-63 -0.18527862374708093 1 64-65 -0.13167046599360788 1 66-67 -0.08860393309188908 1 68-69 -0.2322300666100432 1 70-71 -0.21366113482244486 1 72-73 -0.03379738239673458 1 74-75 -0.19832677457344516 1 76-77 -0.2101817899338485 1 78-79 -0.20201308723513733 1 80 -0.49565414363620164 2 1 -0.03961302524985655 2 2 0.038661164841350626 2 3 0.0856550484411116 2 4 -0.11286286335926121 2 5 -0.0012268059543671939 2 6 -0.774941668839304 2 7 -0.11093673477321175 2 8 -0.04086560555501961 2 9 -0.06578322550279836 2 10-11 -0.41679751777400753 2 12-13 -1.2801050812714792 2 14-15 -1.3588059954790879 2 16-17 -0.7297934038440701 2 18-19 0.07156660998648334 2 20-21 -0.20529083495075895 2 22-23 0.09847365482186632 2 24-25 0.5415518670955208 2 26-27 0.25041341035058196 2 28-29 1.0060298346262542 2 30-31 0.8259394224711869 2 32-33 0.2956415758999569 2 34-35 0.30388759240216956 2 36-37 0.2875875295300432 2 38-39 1.0116067721457185 2 40-41 1.5537049230732407 2 42-43 2.0002463440332647 2 44-45 1.4292998093656095 2 46-47 1.3056314646592782 2 48-49 -8.490010239228241E-4 2 50-51 0.47832901791235827 2 52-53 0.08360064974206693 2 54-55 -0.018124829401259035 2 56-57 0.029016570651776874 2 58-59 0.026480847061691293 2 60-61 -0.03348899391000515 2 62-63 0.0015887196727888409 2 64-65 -0.059455021727430335 2 66-67 -0.05156299648346874 2 68-69 -0.08805114448640694 2 70-71 -0.08814879662635633 2 72-73 -0.1466779286044826 2 74-75 -0.16544086931821056 2 76-77 -0.5365736651523818 2 78-79 -0.37152057193615207 2 80 -0.40152329038094337 3 1 -0.011934703549968617 3 2 -0.21861705571153323 3 3 0.14545241522696273 3 4 0.02473757823374001 3 5 -0.11694501904457866 3 6 -0.24885480885235012 3 7 -0.24589131884471982 3 8 -0.012500563562056755 3 9 0.011051243421484003 3 10-11 -1.0428086871166045 3 12-13 -0.5756588688103434 3 14-15 -0.9079618034909771 3 16-17 -0.6524680481720537 3 18-19 -0.024144340872418013 3 20-21 -0.8996423146111212 3 22-23 -0.6926731918124531 3 24-25 0.30496545502325745 3 26-27 0.5577480326914213 3 28-29 1.4139426600967937 3 30-31 0.9958074379701145 3 32-33 -0.2863404735911459 3 34-35 0.30715792472249603 3 36-37 0.38051625944133605 3 38-39 1.2673294412547236 3 40-41 1.780565896828989 3 42-43 2.3669563065502857 3 44-45 1.7409648185166375 3 46-47 1.6158820347675658 3 48-49 1.6148220769471493 3 50-51 0.7854901638955454 3 52-53 0.4067521805926688 3 54-55 0.36364898986940375 3 56-57 0.1430268487906048 3 58-59 0.17403264249805517 3 60-61 0.0940765550514584 3 62-63 0.11358842363278399 3 64-65 0.09793586363261397 3 66-67 0.04196455142644595 3 68-69 0.053174091073550755 3 70-71 -1.1351057675203862 3 72-73 0.10056576936687378 3 74-75 -0.24971482350293428 3 76-77 0.01788502107758916 3 78-79 -0.10544564606178852 3 80 -0.18372043143629213 4 1 0.03631013319026977 4 2 0.2630154039278523 4 3 0.23592496449014533 4 4 0.2040130425705584 4 5 0.09286740590469833 4 6 -0.06583529681432765 4 7 0.09237150706520225 4 8 -0.07624516336053944 4 9 0.07161484272767638 4 10-11 -0.18120962936262686 4 12-13 -0.5653798296436463 4 14-15 -0.49708447490567664 4 16-17 -0.639054752749395 4 18-19 -0.16985100238617434 4 20-21 -0.22308542328686798 4 22-23 -0.04923822657157473 4 24-25 0.5496115376630186 4 26-27 0.6616688435382869 4 28-29 1.3741753230920182 4 30-31 0.8972858624685145 4 32-33 0.4082532518232682 4 34-35 0.4752072270095553 4 36-37 0.3955604502277552 4 38-39 1.295516307780602 4 40-41 1.861134649043123 4 42-43 2.377645415971365 4 44-45 1.7051879486321582 4 46-47 1.6971672712262276 4 48-49 1.6096542163701173 4 50-51 0.7647829732885612 4 52-53 0.43228764594166336 4 54-55 0.4233443701954158 4 56-57 0.18588450430951298 4 58-59 0.19752636009108926 4 60-61 0.10510851688313494 4 62-63 0.13032286024051842 4 64-65 -0.028553395774892465 4 66-67 0.05697764073278222 4 68-69 0.13547044769158134 4 70-71 0.14646421311020674 4 72-73 -0.3828080971083949 4 74-75 -0.003693834604696633 4 76-77 0.06639509455254 4 78-79 -0.008084947106631546 4 80 0.06383023499112994 5 1 0.08017880108237563 5 2 0.005653581340112623 5 3 -0.20797108421015764 5 4 0.25184967661266455 5 5 0.18009059875308964 5 6 0.034835758090210334 5 7 -1.0914634905599527 5 8 0.011579290519357244 5 9 -0.0940395145905839 5 10-11 -0.15936732486798633 5 12-13 0.05690569254792166 5 14-15 -0.26097408125203714 5 16-17 0.49498924206432804 5 18-19 0.22964900698340074 5 20-21 -1.1238668506794411 5 22-23 0.4510637852629813 5 24-25 0.7372290027480375 5 26-27 0.7972791734758644 5 28-29 1.5451516459430223 5 30-31 1.085124828235216 5 32-33 0.35504091967798246 5 34-35 0.47628475068412257 5 36-37 0.39404735800047774 5 38-39 1.3159959887857084 5 40-41 1.8547631297143568 5 42-43 2.3936280930350264 5 44-45 1.7414918204672958 5 46-47 1.7728537113911145 5 48-49 1.6256089454809342 5 50-51 0.7543863567566049 5 52-53 0.410441299810536 5 54-55 0.29291335721747913 5 56-57 0.14287210059291766 5 58-59 0.17738928269096732 5 60-61 0.13676433135386645 5 62-63 0.18258829469480276 5 64-65 0.1724274370454566 5 66-67 0.1614968232458054 5 68-69 0.062411275882389816 5 70-71 0.24318042804761575 5 72-73 0.3313894643976383 5 74-75 0.2750442472086121 5 76-77 0.1424491993920327 5 78-79 0.19700324812536252 5 80 0.09849256656780625 6 1 -0.21197173177559847 6 2 0.03066421849864298 6 3 0.005112786485256038 6 4 0.03457082921434562 6 5 -0.1727436879805211 6 6 -0.23125575682372812 6 7 0.13553094323215475 6 8 -0.20679696627298938 6 9 -0.02850557690927502 6 10-11 -0.1477591077255198 6 12-13 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3 0.16671492443156666 7 4 0.110273587842741 7 5 0.06473411155815967 7 6 -0.060989853939808825 7 7 -0.023492660000457022 7 8 0.09961326567296425 7 9 0.013672623447796184 7 10-11 -0.09570676350574558 7 12-13 -0.3309594820480797 7 14-15 -0.274921463192328 7 16-17 0.48733012961859856 7 18-19 0.418675285149142 7 20-21 -0.024812177236206878 7 22-23 0.5945535069646937 7 24-25 0.8203665493145991 7 26-27 0.30367046958708244 7 28-29 1.1560369635046044 7 30-31 0.9013507358129189 7 32-33 0.3525499795770415 7 34-35 0.38035801767387767 7 36-37 0.41640643496252494 7 38-39 1.1437074908448892 7 40-41 1.766080252989731 7 42-43 2.301494597693349 7 44-45 1.6308640658518314 7 46-47 1.1953050873152833 7 48-49 1.5170684524466154 7 50-51 0.663825579581399 7 52-53 0.29982238303354336 7 54-55 0.3512086387063036 7 56-57 0.10576550449625444 7 58-59 0.08552356163034602 7 60-61 0.022236630619730136 7 62-63 0.10591219323405454 7 64-65 0.21644581969196253 7 66-67 0.14684956935278137 7 68-69 0.211595758404151 7 70-71 0.33140241401247295 7 72-73 0.3238942438776675 7 74-75 0.21289502813699102 7 76-77 -0.13199533418985254 7 78-79 0.11950962862766801 7 80 0.028810834030451815 8 1 -0.6932325615001318 8 2 -0.30428956678355235 8 3 -0.2195329232025287 8 4 -0.2439706531426964 8 5 -0.21118595414943542 8 6 -0.24757631990414097 8 7 -0.19421140620745803 8 8 -0.18033849310113226 8 9 -0.2942240907612117 8 10-11 -0.17954759431840728 8 12-13 -0.11529463270625939 8 14-15 -0.22652461006384783 8 16-17 0.15398403152397577 8 18-19 0.4788572702151015 8 20-21 0.25485634955690983 8 22-23 0.6904278932561141 8 24-25 0.8137221089045852 8 26-27 0.6172610887918708 8 28-29 1.4194986650491472 8 30-31 1.000158452820429 8 32-33 0.3821056021636693 8 34-35 0.39290101356150586 8 36-37 0.48537581170731414 8 38-39 1.2938101475267274 8 40-41 1.836532470695218 8 42-43 2.399746701143144 8 44-45 1.7116908415122722 8 46-47 1.0667786487873983 8 48-49 1.6516405947915729 8 50-51 0.7973116814635901 8 52-53 0.47151502266788725 8 54-55 0.48557335189447315 8 56-57 0.23984608566990318 8 58-59 0.34305293501043366 8 60-61 0.38694566149333554 8 62-63 0.35027034732071627 8 64-65 0.4506223801558136 8 66-67 0.44655273543645535 8 68-69 0.5995387406226911 8 70-71 0.6833272757273221 8 72-73 0.2565862933185983 8 74-75 0.6337735517254117 8 76-77 0.5586359181859493 8 78-79 0.021029959556564393 8 80 0.19823170331083872 9 1 0.14415134038713973 9 2 0.17250796808879443 9 3 -0.24566693412276663 9 4 0.14920165497278504 9 5 0.2742101831999193 9 6 0.02133418647638763 9 7 -0.017625450514550778 9 8 -0.1524417569878196 9 9 0.1723428206252393 9 10-11 0.12374166083860416 9 12-13 -0.1363357690551581 9 14-15 -0.1496124439738935 9 16-17 0.6661401761689376 9 18-19 0.4858876362371163 9 20-21 0.1347064142442136 9 22-23 0.5396701004074558 9 24-25 -0.9494899971529378 9 26-27 0.37728526393581774 9 28-29 1.3193064834422685 9 30-31 0.7086752255289142 9 32-33 0.4735219924769112 9 34-35 0.4746292269788057 9 36-37 0.4067872347770489 9 38-39 1.2793955508619277 9 40-41 1.8618031528332537 9 42-43 2.444505132036845 9 44-45 1.6305330409912244 9 46-47 1.7987631354604687 9 48-49 1.717357103885746 9 50-51 0.7731283506903281 9 52-53 0.49000115727248783 9 54-55 0.5037978501251459 9 56-57 0.27380734915072225 9 58-59 0.2083497329046935 9 60-61 0.10453378482080211 9 62-63 0.12564804600336998 9 64-65 0.14688009221212894 9 66-67 0.13933796564704437 9 68-69 0.18363588900139405 9 70-71 0.19468944802972032 9 72-73 0.18516911097106004 9 74-75 0.11830652417155108 9 76-77 0.20593643430929376 9 78-79 0.2602984256801264 9 80 0.22085395842856315 10 1 -0.3304885876227175 10 2 -0.07466820794193296 10 3 0.021572258115675425 10 4 0.04242765375400381 10 5 -0.05305174908998822 10 6 0.030334744582482642 10 7 -0.014073125988794288 10 8 0.023044408633857927 10 9 -0.06587628383623212 10 10-11 -0.09477405039092446 10 12-13 -0.08788043336681994 10 14-15 -0.49404755905786146 10 16-17 0.4802967432366074 10 18-19 0.3836143038909441 10 20-21 0.06236980869817188 10 22-23 0.42007698423961415 10 24-25 0.7846187994053793 10 26-27 0.6506147369968005 10 28-29 1.4580382381546357 10 30-31 1.09236748690045 10 32-33 0.49672727913462467 10 34-35 0.42589387091127406 10 36-37 0.4390502314187046 10 38-39 1.3816225163378917 10 40-41 1.9299005718004594 10 42-43 2.4880340942436376 10 44-45 1.7821113423990127 10 46-47 1.6821779752498287 10 48-49 1.7702142025633378 10 50-51 0.7060881945407473 10 52-53 0.6266256052302452 10 54-55 0.5301677424494784 10 56-57 0.38521063803808175 10 58-59 0.32356422259567097 10 60-61 -0.22914431743827635 10 62-63 0.35505173498996356 10 64-65 -0.5281113274263589 10 66-67 0.4361041121461966 10 68-69 0.5868296603894905 10 70-71 0.6885998096875525 10 72-73 0.5723294164371211 10 74-75 0.7611079011759259 10 76-77 0.7511053168450559 10 78-79 0.7401547673570192 10 80 0.4366212613346434 11 1 -0.014311208945343878 11 2 0.260643564869838 11 3 0.27085441364783236 11 4 0.1693711556975046 11 5 0.12657227428403672 11 6 0.05239089163863042 11 7 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0.2581239350925486 97 22-23 0.7612233608481205 97 24-25 0.8953597973615501 97 26-27 0.710493118641871 97 28-29 1.5371105293578609 97 30-31 1.0213953440613253 97 32-33 0.1565300242029295 97 34-35 0.39030309992255496 97 36-37 0.4051959039989157 97 38-39 1.272114497230227 97 40-41 1.8258655912812003 97 42-43 2.3614693420570987 97 44-45 1.6682159526692217 97 46-47 1.7077477552122602 97 48-49 1.5299485273449989 97 50-51 0.6618793567664412 97 52-53 0.361011918965076 97 54-55 0.34028228334867805 97 56-57 0.11523978396500922 97 58-59 0.17018247614588944 97 60-61 0.1642766253648844 97 62-63 0.225025261554471 97 64-65 0.20851822465168368 97 66-67 0.28981256433827696 97 68-69 0.3429055936156189 97 70-71 0.35220336419691023 97 72-73 0.3282846932925949 97 74-75 0.38639453221912845 97 76-77 0.48973537238782683 97 78-79 0.6520620219913269 97 80 0.13874599904185914 98 1 -0.3440596938783571 98 2 -1.1411975511188999 98 3 -0.3894641878834264 98 4 -0.12819518475108538 98 5 -0.16752122578485995 98 6 -0.10956619703198811 98 7 -0.0885891999336863 98 8 -0.4397420000444683 98 9 -0.1960277100553256 98 10-11 -0.06267936390806028 98 12-13 0.06624030330005581 98 14-15 0.18694584647420243 98 16-17 0.8883974225312699 98 18-19 0.6713883712673834 98 20-21 0.20060572067090732 98 22-23 0.7538808420768603 98 24-25 0.8016646182910421 98 26-27 0.6063265827240301 98 28-29 1.4246018768623898 98 30-31 0.9311710351448994 98 32-33 0.071673807228116 98 34-35 -0.00392197933515348 98 36-37 0.3631571056793632 98 38-39 1.23458303632939 98 40-41 1.744172135292139 98 42-43 2.2875194704370543 98 44-45 1.5215020072290386 98 46-47 1.6009941701479633 98 48-49 1.5277284226269856 98 50-51 0.3083158207358565 98 52-53 -0.1612483941771501 98 54-55 0.13529891083855716 98 56-57 0.08215021428894431 98 58-59 0.09625921233811141 98 60-61 0.0909966731003422 98 62-63 0.1995857045294258 98 64-65 0.2953489573311501 98 66-67 0.35843424889856124 98 68-69 0.3494819687360078 98 70-71 0.47342801678618684 98 72-73 0.519981162144667 98 74-75 0.5502105669536732 98 76-77 0.34435449748440305 98 78-79 0.6438677250737115 98 80 0.28488232524816937 99 1 0.01634445256447492 99 2 0.08591329266716485 99 3 -0.18895883463142127 99 4 -0.138875137789924 99 5 0.04234470208435681 99 6 -0.02190316818709448 99 7 -0.4147564294466477 99 8 -0.6725513604148432 99 9 -0.11448188599354836 99 10-11 0.030381611049961066 99 12-13 0.08288921826787998 99 14-15 0.20471003787666575 99 16-17 0.9764637195949746 99 18-19 0.7511623953749691 99 20-21 0.265882921192258 99 22-23 0.8097179512629182 99 24-25 0.6480336214761451 99 26-27 0.6124831033502076 99 28-29 1.4274697889051922 99 30-31 0.7882248930843332 99 32-33 0.17539703458567857 99 34-35 -0.055618908989075067 99 36-37 0.27213660595627687 99 38-39 1.0833286226579517 99 40-41 1.5467157511991587 99 42-43 2.1463544552059943 99 44-45 1.2987943676671634 99 46-47 1.3741800843876888 99 48-49 1.2675263698293833 99 50-51 0.3848445687866615 99 52-53 0.1484190482540768 99 54-55 0.025623196762424527 99 56-57 -0.12525957967901746 99 58-59 -0.20940305197532183 99 60-61 -0.9928447536801173 99 62-63 -0.41630994611714556 99 64-65 -0.2508132547313551 99 66-67 -1.3123841707951698 99 68-69 -2.0883472929396945 99 70-71 -9.540224043789483 99 72-73 -3.361480685999503 99 74-75 -3.4207015980489643 99 76-77 -0.5212885616353944 99 78-79 -0.5391032387295027 99 80 -0.7052292806865808 100 1 -0.3947764714526514 100 2 -0.9609818764965681 100 3 -0.3659343374108701 100 4 -0.29033227334033995 100 5 -0.14879941158828558 100 6 -0.10573766729141454 100 7 -0.10719349566567615 100 8 -0.5033034165073786 100 9 -0.1823909273066775 100 10-11 -0.07827195467848469 100 12-13 0.03500808715426729 100 14-15 0.23381325128204367 100 16-17 0.892615559665586 100 18-19 0.6646988691367071 100 20-21 0.19940193555563468 100 22-23 0.7548987071290512 100 24-25 0.6425311369810771 100 26-27 0.4417335283442796 100 28-29 1.3633506951281475 100 30-31 0.8766138194737465 100 32-33 0.09985169330162336 100 34-35 0.20486939531309645 100 36-37 0.012696419008150883 100 38-39 0.9058501936339383 100 40-41 1.3695105656741546 100 42-43 2.095234574649126 100 44-45 1.2819892985291403 100 46-47 1.2967437919896057 100 48-49 1.3112442430144 100 50-51 0.44669959004092163 100 52-53 0.29328413377023566 100 54-55 0.23980714233672984 100 56-57 0.0805865110133368 100 58-59 -0.2244476227793939 100 60-61 -0.08029036095204845 100 62-63 -0.19335336830500438 100 64-65 0.018955960342879052 100 66-67 0.23001043145107758 100 68-69 0.3469090152533809 100 70-71 0.45416541350927275 100 72-73 0.16246583592001684 100 74-75 0.437405463807913 100 76-77 0.19484230798883573 100 78-79 -0.46842907498763964 100 80 -1.2149158383199818 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3537.0 5 312.0 6 374.0 7 564.0 8 773.0 9 1364.0 10 1920.0 11 2910.0 12 3972.0 13 5154.0 14 5571.0 15 5547.0 16 6248.0 17 6618.0 18 7281.0 19 8213.0 20 9529.0 21 11487.0 22 13535.0 23 16995.0 24 21199.0 25 27460.0 26 36027.0 27 49802.0 28 69608.0 29 96870.0 30 129485.0 31 167249.0 32 210191.0 33 259223.0 34 320852.0 35 422000.0 36 595013.0 37 982526.0 38 1556473.0 39 2874439.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.959235649668393 13.71661261903083 11.246142255126621 50.07800947617416 2 24.52817459797051 21.518716148667938 35.066524897531785 18.88658435582977 3 23.15523021036247 26.961663046800684 23.882061638936776 26.001045103900072 4 26.28510749533653 31.64149533224106 18.32462806032243 23.748769112099982 5 26.445529925815165 33.43161007106012 21.359926267857553 18.762933735267158 6 18.99241985860104 35.94049778779614 23.43371212813797 21.63337022546485 7 18.194861321607544 15.561552505968542 42.34782500141545 23.89576117100846 8 20.05860540176969 20.71596795441096 27.77891756544213 31.446509078377222 9 22.031033524847086 19.59158808962102 31.03724474413224 27.340133641399657 10-11 24.47852744488852 29.645595899093568 20.74634872737013 25.129527928647775 12-13 22.95326703528061 23.414999127391727 27.525652413392372 26.10608142393529 14-15 23.408360084016714 25.017776694454085 26.422586357409976 25.15127686411922 16-17 24.454443756298215 25.02605978179831 24.92101993064974 25.598476531253738 18-19 24.062531839921235 25.07628340732805 24.99706190033764 25.864122852413075 20-21 24.02209370876907 25.749527575485292 25.494837366595686 24.733541349149952 22-23 23.797600023302948 25.55600796789338 25.231878836774325 25.414513172029352 24-25 23.74581779540743 25.65476808207278 25.09629174181594 25.503122380703847 26-27 23.78669709196193 25.94536977332411 25.60084693490181 24.66708619981215 28-29 24.104536701312487 24.630617409181298 24.947204036490533 26.317641853015683 30-31 23.700906638912127 25.117318180559174 25.307041223403836 25.874733957124864 32-33 23.823120312378137 26.09842387901381 25.531450070271966 24.547005738336086 34-35 24.046278019328014 25.98845540158764 25.30973970269301 24.65552687639133 36-37 23.968480135147463 25.86282827491846 25.399321248923666 24.769370341010408 38-39 24.0302044904712 25.235582049439458 25.292550121026412 25.441663339062927 40-41 23.966768822086756 24.905197996171474 24.97867750999726 26.149355671744505 42-43 23.899972045385482 24.849855090668992 25.124923957205542 26.12524890673999 44-45 23.864479624749766 25.477394755321054 25.238163520182717 25.419962099746467 46-47 24.16150228157046 25.20881050645586 25.034179254241117 25.595507957732565 48-49 23.86162429794408 25.22157632471404 25.22677901621455 25.69002036112733 50-51 24.111078192459615 25.96462805079768 25.513536742857966 24.410757013884744 52-53 24.294540439505205 25.692326432440932 25.34387926248186 24.669253865572 54-55 23.898770119994428 25.787916802668843 25.547787975690532 24.765525101646194 56-57 24.133561285836727 25.80449911964316 25.601839878330058 24.460099716190054 58-59 24.259971959533306 25.688728122033343 25.473390149419483 24.577909769013868 60-61 23.981036872880328 25.719371881104035 25.599073691959813 24.700517554055818 62-63 24.051785512978913 25.849750691207397 25.707896497571475 24.390567298242217 64-65 24.196212014171703 25.898428050002337 25.375743531825584 24.52961640400037 66-67 24.078251778978274 25.744671608330783 25.59494255247689 24.582134060214056 68-69 24.19807511041477 25.93189434228736 25.49474327975209 24.37528726754578 70-71 24.216734856402862 25.744638732342125 25.50599147809139 24.532634933163628 72-73 24.13952813725783 25.706883377421107 25.50778964633102 24.645798838990043 74-75 24.180219806825114 25.84711828402536 25.59137220949401 24.38128969965551 76-77 24.295211269026794 25.838481192590105 25.543701281113 24.322606257270092 78-79 24.0648852047786 25.7928740430787 25.69572278278765 24.446517969355046 80 24.526138707773338 25.939043875492146 25.37518314188053 24.15963427485399 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8.0 1 7.5 2 5.0 3 2.5 4 5.0 5 8.0 6 13.5 7 19.5 8 23.5 9 35.0 10 43.0 11 61.5 12 94.0 13 150.5 14 246.0 15 299.0 16 350.0 17 497.5 18 781.0 19 1202.0 20 1436.0 21 1852.5 22 2865.5 23 4358.0 24 6860.5 25 8467.0 26 10276.0 27 15106.5 28 21255.0 29 29042.5 30 33703.0 31 38766.5 32 51169.0 33 68231.0 34 90784.0 35 103614.0 36 116566.0 37 146050.0 38 177451.0 39 208625.5 40 224931.0 41 243795.0 42 286834.0 43 338193.0 44 387431.0 45 409485.0 46 434844.0 47 480026.0 48 509293.5 49 521477.0 50 524216.0 51 521606.0 52 509594.0 53 482261.0 54 443547.0 55 422764.0 56 392195.0 57 329634.0 58 268440.0 59 213203.5 60 187169.0 61 166941.5 62 125616.5 63 90345.0 64 64086.5 65 52002.0 66 43303.0 67 29034.5 68 19623.0 69 13124.0 70 10467.0 71 8541.5 72 5553.5 73 3610.0 74 2129.5 75 1530.0 76 1209.0 77 766.0 78 504.5 79 277.5 80 190.0 81 156.0 82 107.0 83 83.0 84 57.0 85 40.0 86 32.0 87 21.0 88 15.5 89 12.5 90 12.0 91 11.0 92 7.5 93 7.0 94 6.0 95 3.0 96 5.0 97 5.5 98 3.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03081840130930288 2 7.944186916882494E-4 3 1.8914730754482129E-4 4 9.583463582270946E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.00962129304377991 12-13 9.961758197360587E-4 14-15 0.0 16-17 4.41343717604583E-5 18-19 3.9090443559263073E-4 20-21 0.01617209479508222 22-23 0.0 24-25 0.0 26-27 0.0010529200119995053 28-29 0.0034046515358067833 30-31 0.07083566667553558 32-33 8.385530634487077E-4 34-35 0.008127029314175822 36-37 3.341602433291843E-4 38-39 0.0056302848545841805 40-41 0.011424497375707205 42-43 0.017697883075943777 44-45 0.05702791322476362 46-47 0.024040622788946784 48-49 0.021922172944444786 50-51 4.41343717604583E-5 52-53 0.0 54-55 6.3049102514940426E-6 56-57 2.5219641005976175E-4 58-59 3.152455125747022E-5 60-61 0.03710439683004244 62-63 1.2609820502988085E-5 64-65 2.2067185880229152E-4 66-67 3.152455125747022E-5 68-69 2.521964100597617E-5 70-71 0.05552103967465654 72-73 0.06830109275443497 74-75 0.0 76-77 0.023574059430336226 78-79 0.0767433675811855 80 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 80 7930327.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.38338825408633 #Duplication Level Percentage of deduplicated Percentage of total 1 76.50213863615363 43.13449784990189 2 12.699549707681873 14.320872836405913 3 4.408627756462844 7.457201113811583 4 2.0221954992772395 4.560729358456582 5 1.139915022472755 3.2136135644373445 6 0.7088650575711009 2.3980928256471983 7 0.4981098051042636 1.9659582974052676 8 0.33654485974914927 1.5180431593722652 9 0.24693146932964274 1.253054961662873 >10 1.3259909544056578 13.136953728860167 >50 0.0774391638549893 2.9758975929881766 >100 0.032027419793323256 3.178997028679379 >500 0.0011472575044280343 0.44605619177561384 >1k 5.173906392518586E-4 0.4400314905957509 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.011020983119611588 0.0 0.0 1.2609820502988087E-5 0.0 2 0.011638864324258003 1.2609820502988087E-5 0.0 1.2609820502988087E-5 1.2609820502988087E-5 3 0.013845582912280918 1.2609820502988087E-5 0.0 1.2609820502988087E-5 2.5219641005976174E-5 4 0.018864291472470176 1.2609820502988087E-5 0.0 1.2609820502988087E-5 2.5219641005976174E-5 5 0.01977219854868532 1.2609820502988087E-5 0.0 1.2609820502988087E-5 2.5219641005976174E-5 6 0.023731682186623578 1.2609820502988087E-5 0.0 1.2609820502988087E-5 2.5219641005976174E-5 7 0.024412612493784933 1.2609820502988087E-5 0.0 1.2609820502988087E-5 3.782946150896426E-5 8 0.02596362041565247 1.2609820502988087E-5 0.0 1.2609820502988087E-5 5.043928201195235E-5 9 0.027728995286070802 1.2609820502988087E-5 0.0 1.2609820502988087E-5 5.043928201195235E-5 10 0.03185240659054791 1.2609820502988087E-5 0.0 1.2609820502988087E-5 6.304910251494042E-5 11 0.03426088230661863 1.2609820502988087E-5 0.0 1.2609820502988087E-5 1.008785640239047E-4 12 0.03663152856118039 1.2609820502988087E-5 0.0 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69 0.14600911160409905 3.782946150896426E-5 2.5219641005976174E-5 1.5131784603585703E-4 9.961758197360587E-4 >>END_MODULE