##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename SRR949624_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4463141 Sequences flagged as poor quality 0 Sequence length 78 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.06081703446071 40.0 40.0 40.0 38.0 40.0 2 38.78974717581183 40.0 39.0 40.0 36.0 40.0 3 38.4084488928313 40.0 39.0 40.0 35.0 40.0 4 38.18285126999125 40.0 38.0 40.0 35.0 40.0 5 38.08550614914474 40.0 38.0 40.0 35.0 40.0 6 38.23546063187338 40.0 39.0 40.0 35.0 40.0 7 38.20443337998957 40.0 39.0 40.0 35.0 40.0 8 38.19920970455561 40.0 39.0 40.0 35.0 40.0 9 38.19536823954251 40.0 39.0 40.0 35.0 40.0 10-11 38.511780716764264 40.0 39.5 40.0 36.0 40.0 12-13 38.82481586398458 40.0 40.0 40.0 37.5 40.0 14-15 38.82406863238244 40.0 40.0 40.0 37.0 40.0 16-17 38.91502060992471 40.0 40.0 40.0 38.0 40.0 18-19 38.8575569313181 40.0 40.0 40.0 37.5 40.0 20-21 38.819421792858435 40.0 39.0 40.0 37.0 40.0 22-23 38.76654031320095 40.0 39.0 40.0 37.0 40.0 24-25 38.67263738698823 40.0 39.0 40.0 36.0 40.0 26-27 38.53204816966347 40.0 39.0 40.0 36.0 40.0 28-29 38.4026000971065 40.0 39.0 40.0 35.5 40.0 30-31 38.34290054918722 40.0 38.5 40.0 35.0 40.0 32-33 38.28008559442778 40.0 38.0 40.0 35.0 40.0 34-35 38.18130325705596 40.0 38.0 40.0 35.0 40.0 36-37 38.06969105390128 40.0 38.0 40.0 35.0 40.0 38-39 37.953999772805744 40.0 38.0 40.0 35.0 40.0 40-41 37.8188644499468 40.0 38.0 40.0 34.0 40.0 42-43 37.623354157979776 40.0 38.0 40.0 33.0 40.0 44-45 37.56292082190547 39.5 38.0 40.0 33.0 40.0 46-47 37.61741282652733 40.0 38.0 40.0 33.0 40.0 48-49 37.85348726379023 40.0 38.0 40.0 34.0 40.0 50-51 37.86277601357429 40.0 38.0 40.0 34.5 40.0 52-53 37.7942320217981 40.0 38.0 40.0 33.5 40.0 54-55 37.62237827574795 40.0 38.0 40.0 33.0 40.0 56-57 37.51001716056024 40.0 38.0 40.0 33.0 40.0 58-59 37.43085508613777 40.0 38.0 40.0 33.0 40.0 60-61 37.282938069848115 40.0 37.0 40.0 33.0 40.0 62-63 37.07579337063292 39.5 37.0 40.0 33.0 40.0 64-65 36.90471452728023 39.0 36.5 40.0 32.0 40.0 66-67 36.6610873373707 39.0 36.0 40.0 31.5 40.0 68-69 36.43470921039689 39.0 36.0 40.0 31.0 40.0 70-71 36.21922677325229 39.0 36.0 40.0 31.0 40.0 72-73 35.91966812162107 39.0 35.0 40.0 30.0 40.0 74-75 35.6715820763897 39.0 35.0 40.0 29.0 40.0 76-77 35.345354202343145 38.0 35.0 40.0 29.0 40.0 78 34.708830619512135 38.0 34.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.26805826773403396 1 2 -0.24115723104548437 1 3 -0.4429077491781612 1 4 -1.84243657556096 1 5 -0.36832111227520414 1 6 -0.29189015372375593 1 7 -1.2440042578594657 1 8 -0.18722220287754965 1 9 -0.05561218567707016 1 10-11 -0.12367062244025107 1 12-13 -0.15871126183090212 1 14-15 -0.19035775188711312 1 16-17 -0.1689522736163127 1 18-19 -0.12503760824026244 1 20-21 -0.06370880637132359 1 22-23 -0.09232615648587483 1 24-25 -0.05027493960135132 1 26-27 -0.06555350179712605 1 28-29 -0.09275535253344458 1 30-31 -0.027016192386085436 1 32-33 -0.1070666558386435 1 34-35 -0.043167702705737554 1 36-37 -0.02580150034746964 1 38-39 0.01339801138017549 1 40-41 0.03122124499385137 1 42-43 0.11285958779347283 1 44-45 0.1580882820576619 1 46-47 0.16286035734464122 1 48-49 0.06988811249529192 1 50-51 0.06259699331041446 1 52-53 0.12278987356260274 1 54-55 0.1326964880248127 1 56-57 0.031893573910330986 1 58-59 0.14327241204231456 1 60-61 0.18961466560743645 1 62-63 0.21923971902883466 1 64-65 0.20361400110131456 1 66-67 0.37771736522669386 1 68-69 0.2891833515158311 1 70-71 0.32008277523515005 1 72-73 0.3262802534013929 1 74-75 0.2613049808564085 1 76-77 0.4417846590524235 1 78 0.1998831131688732 2 1 -0.12391002100834925 2 2 -0.28346199218044177 2 3 -0.5665578275537229 2 4 0.1073763623246009 2 5 -0.9633381297314543 2 6 0.053989283588784076 2 7 -0.5111891462428488 2 8 -0.42318604908247437 2 9 -0.3281779966115437 2 10-11 -0.5180671306141207 2 12-13 -0.3630722917117737 2 14-15 -0.3002415886234573 2 16-17 -0.4363089242807021 2 18-19 -0.22525945582505358 2 20-21 -0.21666889112661636 2 22-23 -0.21870963670723853 2 24-25 -0.21875749923461285 2 26-27 -0.2450948820730403 2 28-29 -0.28686693739411595 2 30-31 -0.26093873770101794 2 32-33 -0.30266167502117725 2 34-35 -0.40756246438918 2 36-37 -0.5284338655573748 2 38-39 -0.2662158265974739 2 40-41 -0.23328689422885418 2 42-43 -0.25162718939764517 2 44-45 -0.3358235389400619 2 46-47 -0.3966819755624087 2 48-49 -0.7363665604491132 2 50-51 -0.38398638067813806 2 52-53 -0.30360024620911474 2 54-55 -0.4038084093652685 2 56-57 -0.29023792382789537 2 58-59 -0.24906330960463663 2 60-61 -0.25899160616955896 2 62-63 -0.15395533364012692 2 64-65 -0.11528143752758524 2 66-67 -0.2689939644442987 2 68-69 -0.13981423515194535 2 70-71 -0.13917961402147938 2 72-73 -0.16426776931164255 2 74-75 -0.1871822120283042 2 76-77 -0.21639072034014362 2 78 -0.29130937894304765 3 1 0.03976864244997813 3 2 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0.2884882442176746 97 4 0.47558026200461256 97 5 0.4204498593484871 97 6 0.460664885923066 97 7 0.4502100475810167 97 8 0.2789299789395443 97 9 0.2797024954694791 97 10-11 0.13064216593716083 97 12-13 0.013131131694208875 97 14-15 0.0010178137746876814 97 16-17 -0.041435562875548726 97 18-19 -0.05711704825080233 97 20-21 -0.05034865770483066 97 22-23 -0.13434548220177334 97 24-25 -0.04284029080010043 97 26-27 0.04195303646854143 97 28-29 0.06027557814868345 97 30-31 0.030327245208489728 97 32-33 -0.07512642509035317 97 34-35 -0.009844319986896721 97 36-37 -0.021577125083794613 97 38-39 -0.08140857913862476 97 40-41 -0.06310602723972636 97 42-43 -0.10972804218538812 97 44-45 -0.06668310216056028 97 46-47 0.07076642685522927 97 48-49 0.01468869290917496 97 50-51 0.15096970855991998 97 52-53 0.16006436217386977 97 54-55 0.28477325246247176 97 56-57 0.2618503706932884 97 58-59 0.1905218535827018 97 60-61 0.06366836382533592 97 62-63 -0.31681412223949224 97 64-65 0.03597247223658684 97 66-67 0.10833245890155041 97 68-69 0.1287099903538973 97 70-71 0.2796584452707904 97 72-73 0.37084244954340306 97 74-75 0.28217751232585186 97 76-77 0.7137853683441477 97 78 0.668684708181182 98 1 0.12737261789365562 98 2 0.2280676549011602 98 3 0.44953118679745074 98 4 0.4711165932833836 98 5 0.2764394630283249 98 6 0.5615448855012559 98 7 0.5754682260072954 98 8 0.6294178876331813 98 9 0.5892146299154319 98 10-11 0.2833808384810439 98 12-13 0.044380387736104865 98 14-15 0.011275907519568307 98 16-17 -0.1343055802735762 98 18-19 -0.1703904710875861 98 20-21 -0.11095945349754288 98 22-23 -0.1523092842562761 98 24-25 -0.05150996908903949 98 26-27 -0.004505499281322045 98 28-29 -0.056662592282386015 98 30-31 0.01580941713634587 98 32-33 -0.021319740276304344 98 34-35 -0.017506069859429374 98 36-37 -0.03965820289595001 98 38-39 0.04528952270370468 98 40-41 -0.14530680669211904 98 42-43 -0.13101339247801747 98 44-45 -0.17861855879225885 98 46-47 0.11537617179963178 98 48-49 -0.09897094903547554 98 50-51 0.16636199197483847 98 52-53 0.22471482049999025 98 54-55 0.3207315336088925 98 56-57 0.35284834984344826 98 58-59 0.2911834789895522 98 60-61 0.4287153228601426 98 62-63 0.4453775036323009 98 64-65 0.34357356306091447 98 66-67 0.543122823691462 98 68-69 0.5646578141456331 98 70-71 0.5454957254766057 98 72-73 0.7219872782337191 98 74-75 0.8260383860494187 98 76-77 0.6986027512956667 98 78 0.8758327215314452 99 1 0.008667903528952081 99 2 -0.04522163943195778 99 3 -0.3824643754334147 99 4 0.49269269570828556 99 5 0.47992879239439645 99 6 0.33682974402677246 99 7 0.3360458343000232 99 8 0.4193261212994557 99 9 0.11944381330823717 99 10-11 0.15148737397093015 99 12-13 -0.07282422570780511 99 14-15 -0.1964764185136687 99 16-17 -0.41116835367921567 99 18-19 -0.3356585526331628 99 20-21 -0.24280030972963118 99 22-23 -0.19255273386854554 99 24-25 -0.1517567886126443 99 26-27 -0.09645384352305086 99 28-29 -0.09599691121997722 99 30-31 -0.37868646629531355 99 32-33 -0.13719331720962913 99 34-35 -0.4263923377061758 99 36-37 -0.4394718181615218 99 38-39 -0.14288550162419256 99 40-41 -0.30181152996217975 99 42-43 -0.41009206892278627 99 44-45 -0.10344637064293494 99 46-47 -0.03622325127469139 99 48-49 -0.12355150982559593 99 50-51 0.0526986492542747 99 52-53 0.0675995705135719 99 54-55 0.04123359939592319 99 56-57 0.2808056284694018 99 58-59 0.14105763692854367 99 60-61 0.22126911345134204 99 62-63 0.3111018570477313 99 64-65 0.18592901338247714 99 66-67 0.3270147456531092 99 68-69 0.4888133110339581 99 70-71 0.2703285000022717 99 72-73 0.576974031029934 99 74-75 0.6821438395584707 99 76-77 0.851908157649504 99 78 0.8064869688260572 100 1 -0.06255959483868878 100 2 -0.0627180552039519 100 3 0.3639298640867352 100 4 0.5164703597133453 100 5 0.06665680854840161 100 6 0.27795596229638164 100 7 0.25177542511324447 100 8 0.4353182069522461 100 9 0.3410068852646688 100 10-11 0.03618655442006258 100 12-13 -0.2284359145898378 100 14-15 -0.15714310815864252 100 16-17 -0.16934862311401133 100 18-19 -0.2810231640842602 100 20-21 -0.1815459887113775 100 22-23 -0.16012178302379 100 24-25 -0.11062607795568624 100 26-27 -0.06802102059204884 100 28-29 -0.09034268020437963 100 30-31 -0.08322989644992873 100 32-33 -0.07877086549698475 100 34-35 -0.24795974714004387 100 36-37 -0.19207505980627104 100 38-39 -0.09921020208652465 100 40-41 -0.12061747664374423 100 42-43 -0.27697372472380977 100 44-45 -0.010479919481767297 100 46-47 0.06007513773972306 100 48-49 -0.055870092895645485 100 50-51 -0.1595050371368103 100 52-53 0.0115719485278305 100 54-55 0.1215818639789461 100 56-57 0.13066944329629138 100 58-59 0.13260015621289512 100 60-61 0.22465575386594594 100 62-63 0.2586276553565767 100 64-65 0.28076164484483 100 66-67 0.2757759311166694 100 68-69 0.31931662107142245 100 70-71 0.4032830657007267 100 72-73 0.42515085759202265 100 74-75 0.5543951762373425 100 76-77 0.7886378778125049 100 78 0.6558321646437975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 7868.0 5 1002.0 6 906.0 7 1119.0 8 1182.0 9 1387.0 10 1603.0 11 1702.0 12 2100.0 13 2203.0 14 2822.0 15 3106.0 16 3604.0 17 4345.0 18 4925.0 19 6245.0 20 7427.0 21 9313.0 22 9648.0 23 10224.0 24 11442.0 25 13277.0 26 15334.0 27 17581.0 28 21867.0 29 26678.0 30 33778.0 31 42905.0 32 56482.0 33 74404.0 34 100801.0 35 147407.0 36 226691.0 37 390198.0 38 839794.0 39 2360470.0 40 1301.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.88374253355879 12.076214554919556 9.17755829588023 49.86248461564142 2 25.83312084727936 17.164630908363314 33.35129568692895 23.65095255742837 3 24.328674016675826 20.70582630124979 22.05101538616287 32.914484295911514 4 29.336654391460186 25.205709041328383 18.452062137796723 27.005574429414708 5 28.929964557231973 29.140691907712807 21.130341688705915 20.79900184634931 6 22.929583086148078 33.68889546541194 22.25024786402526 21.131273584414725 7 20.431169346150433 20.185326182410105 37.4620665492148 21.92143792222466 8 20.464412221564793 23.303311525003025 29.16277959482438 27.069496658607804 9 22.259260138960048 20.98565949471746 31.615639622276586 25.139440744045906 10-11 24.325414862267287 29.417462092653945 22.470425688099084 23.786697356979687 12-13 23.953765947144998 24.07207602833046 26.163767185758203 25.810390838766338 14-15 23.833747968482704 25.112753356958972 26.02078577756265 25.03271289699568 16-17 24.75223826498881 25.040517891504688 25.110974502509098 25.096269340997406 18-19 24.473288091438945 25.353223937483847 25.09514210071825 25.07834587035896 20-21 24.28388871422844 25.592620663988725 25.397940140684778 24.72555048109806 22-23 24.367493723057184 25.432065472348025 25.32745444054728 24.872986364047513 24-25 24.053193060476822 25.64242335281409 25.406116399466537 24.89826718724255 26-27 24.125382967090502 25.788589681194345 25.467875304936786 24.618152046778363 28-29 24.56867669618882 25.475491782142974 25.17079003299923 24.785041488668973 30-31 24.21247551075952 25.389927190198456 25.348675050552988 25.048922248489035 32-33 24.157562860965857 25.654737139124006 25.39602341094146 24.791676588968677 34-35 24.384063288522455 25.681081839191304 25.109457626246968 24.825397246039273 36-37 24.174608039613922 25.58241192906763 25.224897235287784 25.01808279603066 38-39 24.182680261897463 25.66335044227847 25.350344747953677 24.80362454787039 40-41 24.592381303423053 25.491235804501667 25.167287432338036 24.749095459737244 42-43 24.305704154739832 25.33889389924882 25.441549638974003 24.913852307037345 44-45 24.385385886382004 25.46009015195594 25.4191160644529 24.735407897209157 46-47 24.3486280761782 25.498221310479813 25.256449962507904 24.89670065083408 48-49 24.12867286999618 25.413248118503024 25.38820668248927 25.069872329011528 50-51 24.344288357391722 25.571348001029843 25.385501508798352 24.698862132780082 52-53 24.404341642767882 25.60424018450704 25.257012282768297 24.734405889956776 54-55 24.25636486872546 25.43386418252399 25.326805550109512 24.982965398641042 56-57 24.26179284019215 25.576542463814782 25.449686652814812 24.711978043178252 58-59 24.388751857763 25.608172644653425 25.231617459638993 24.771458037944583 60-61 24.17076128880461 25.60719077456563 25.311246369179834 24.910801567449926 62-63 24.212818950011393 25.786839266305854 25.385417132416972 24.61492465126578 64-65 24.36913173161489 25.78877864710106 25.19000571081747 24.652083910466573 66-67 24.248518374531653 25.656005134516 25.205894472785868 24.88958201816648 68-69 24.308056842821426 25.867855067281866 25.238710371623647 24.58537771827306 70-71 24.43874046896631 25.748058376908894 25.180331999167937 24.632869154956854 72-73 24.329704185913066 25.637822332229216 25.230477665585337 24.801995816272377 74-75 24.371637796546345 25.81403428550935 25.30309143400219 24.511236483942117 76-77 24.581135639255635 25.687295093129475 25.21824322484059 24.513326042774295 78 24.35917719036963 25.68958508591438 25.31109480722094 24.64014291649505 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 68.0 1 50.5 2 33.0 3 37.0 4 38.5 5 36.0 6 44.5 7 61.5 8 74.0 9 78.0 10 99.5 11 131.0 12 172.0 13 277.0 14 351.0 15 395.0 16 513.5 17 735.5 18 883.0 19 1014.0 20 1377.0 21 1940.0 22 2689.0 23 3107.0 24 3779.0 25 5605.5 26 8203.5 27 9647.0 28 11049.0 29 14487.0 30 19686.0 31 26254.0 32 29659.0 33 33721.0 34 43258.5 35 55434.5 36 62135.0 37 70055.0 38 89408.5 39 110263.5 40 131073.5 41 142462.0 42 154265.0 43 182896.5 44 216655.5 45 233586.0 46 246436.0 47 269960.0 48 288426.0 49 300845.0 50 305472.0 51 303490.0 52 294888.5 53 278615.0 54 255264.0 55 241567.0 56 225401.0 57 191777.0 58 158327.5 59 142336.0 60 126022.0 61 97124.0 62 72965.0 63 52993.5 64 44597.0 65 37439.5 66 25240.0 67 17136.0 68 14074.0 69 11770.0 70 8020.0 71 5467.5 72 3447.5 73 2534.0 74 2111.5 75 1383.0 76 881.0 77 685.0 78 569.5 79 369.5 80 237.0 81 180.0 82 171.0 83 137.0 84 93.0 85 71.0 86 59.0 87 54.0 88 44.0 89 33.0 90 23.0 91 19.0 92 21.5 93 22.0 94 16.0 95 12.0 96 11.0 97 7.5 98 5.0 99 3.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 8.962298076623616E-5 2 0.002307791754730581 3 0.20767885218056073 4 0.5645799673369046 5 0.8193332901649311 6 0.9719388206646395 7 1.005502626961595 8 0.9763527524673767 9 0.951034260400915 10-11 0.7703543311761828 12-13 0.5084087641416661 14-15 0.38682398785967104 16-17 0.35228553164688275 18-19 0.3520950828127545 20-21 0.33222118682784163 22-23 0.2985229460597369 24-25 0.3243903788833918 26-27 0.39775799151315183 28-29 0.4211159808753522 30-31 0.3878658550110785 32-33 0.26638190458244543 34-35 0.2328068954128942 36-37 0.17789041394838298 38-39 0.06718362695688977 40-41 0.005343770228186831 42-43 0.0037193537017988004 44-45 0.012648043160635078 46-47 0.019425781081081686 48-49 0.012043088040462984 50-51 0.0071250269709157744 52-53 0.004974075432526107 54-55 0.0037193537017988004 56-57 0.004481149038311808 58-59 0.010048976718414229 60-61 0.010721149074161 62-63 0.028108007342810812 64-65 0.0321074328595041 66-67 0.03521062856853503 68-69 0.05026728933726271 70-71 0.04313105949375115 72-73 0.04052079017893452 74-75 0.03990463218616665 76-77 0.04751138267870094 78 0.015123878004302352 >>END_MODULE >>Sequence Length Distribution pass #Length Count 78 4463141.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.42253566687393 #Duplication Level Percentage of deduplicated Percentage of total 1 82.81112105547182 59.14580247198249 2 10.803827621746654 15.432735273059162 3 3.187346717442247 6.829451538276371 4 1.2389561770925102 3.5395756699233427 5 0.612112009875606 2.185929592873117 6 0.339833817990122 1.4563075791725655 7 0.22456367833525095 1.1227235127768707 8 0.152408728152118 0.8708334257910032 9 0.1096303014568864 0.7047066704377147 >10 0.48295695041936354 5.910581321346229 >50 0.02731982976694112 1.3340055334656427 >100 0.0095441354633198 1.1564096988037393 >500 3.158139893052261E-4 0.14760800782939762 >1k 6.316279786104522E-5 0.16332970426232704 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.10053457867452541 2.240574519155904E-5 0.0 0.0 0.0 2 0.10098269357835658 2.240574519155904E-5 0.0 0.0 0.0 3 0.10264071872253196 2.240574519155904E-5 0.0 0.0 2.240574519155904E-5 4 0.10947447100595746 2.240574519155904E-5 0.0 0.0 4.481149038311808E-5 5 0.11245443511643481 2.240574519155904E-5 0.0 0.0 4.481149038311808E-5 6 0.11763016225568496 4.481149038311808E-5 0.0 0.0 4.481149038311808E-5 7 0.11980351953926618 4.481149038311808E-5 0.0 0.0 4.481149038311808E-5 8 0.12403820538047083 4.481149038311808E-5 0.0 0.0 4.481149038311808E-5 9 0.12726463268805535 4.481149038311808E-5 0.0 0.0 4.481149038311808E-5 10 0.1325972000436464 4.481149038311808E-5 0.0 0.0 4.481149038311808E-5 11 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